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Protein

Type-2 restriction enzyme PstI

Gene

pstIR

Organism
Providencia stuartii
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Recognizes the double-stranded sequence CTGCAG and cleaves after A-5.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium

Protein family/group databases

REBASEi1536 PstI

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme PstI (EC:3.1.21.4)
Short name:
R.PstI
Alternative name(s):
Endonuclease PstI
Type II restriction enzyme PstI
Gene namesi
Name:pstIR
OrganismiProvidencia stuartii
Taxonomic identifieri588 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesMorganellaceaeProvidencia

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5713

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000773541 – 326Type-2 restriction enzyme PstIAdd BLAST326

Interactioni

Subunit structurei

Homodimer.

Chemistry databases

BindingDBiP00640

Structurei

3D structure databases

ProteinModelPortaliP00640
SMRiP00640
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

InterProiView protein in InterPro
IPR009528 Restrct_endonuc_II_BsuBI_C
PfamiView protein in Pfam
PF06616 BsuBI_PstI_RE, 1 hit

Sequencei

Sequence statusi: Complete.

P00640-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKELKLKEAK EILKALGLPP QQYNDRSGWV LLALANIKPE DSWKEAKAPL
60 70 80 90 100
LPTVSIMEFI RTEYGKDYKP NSRETIRRQT LHQFEQARIV DRNRDLPSRA
110 120 130 140 150
TNSKDNNYSL NQVIIDILHN YPNGNWKELI QQFLTHVPSL QELYERALAR
160 170 180 190 200
DRIPIKLLDG TQISLSPGEH NQLHADIVHE FCPRFVGDMG KILYIGDTAS
210 220 230 240 250
SRNEGGKLMV LDSEYLKKLG VPPMSHDKLP DVVVYDEKRK WLFLIEAVTS
260 270 280 290 300
HGPISPKRWL ELEAALSSCT VGKVYVTAFP TRTEFRKNAA NIAWETEVWI
310 320
ADNPDHMVHF NGDRFLGPHD KKPELS
Length:326
Mass (Da):37,414
Last modified:July 21, 1986 - v1
Checksum:i475841183B414504
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02081 Genomic DNA Translation: AAA25673.1
PIRiA00783 NDOFS

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K02081 Genomic DNA Translation: AAA25673.1
PIRiA00783 NDOFS

3D structure databases

ProteinModelPortaliP00640
SMRiP00640
ModBaseiSearch...
MobiDBiSearch...

Chemistry databases

BindingDBiP00640
ChEMBLiCHEMBL5713

Protein family/group databases

REBASEi1536 PstI

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiView protein in InterPro
IPR009528 Restrct_endonuc_II_BsuBI_C
PfamiView protein in Pfam
PF06616 BsuBI_PstI_RE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiT2PS_PROST
AccessioniPrimary (citable) accession number: P00640
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 12, 2018
This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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