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Protein

Alkaline phosphatase

Gene

phoA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+Note: Binds 1 Mg2+ ion.
  • Zn2+Note: Binds 2 Zn2+ ions.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi73Magnesium1
Metal bindingi73Zinc 21
Active sitei124Phosphoserine intermediate1
Metal bindingi175Magnesium1
Metal bindingi177Magnesium1
Metal bindingi344Magnesium1
Metal bindingi349Zinc 11
Metal bindingi353Zinc 11
Metal bindingi391Zinc 21
Metal bindingi392Zinc 21
Metal bindingi434Zinc 11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMagnesium, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciEcoCyc:ALKAPHOSPHA-MONOMER
MetaCyc:ALKAPHOSPHA-MONOMER
BRENDAi3.1.3.1 2026
SABIO-RKiP00634

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase (EC:3.1.3.1)
Short name:
APase
Gene namesi
Name:phoA
Ordered Locus Names:b0383, JW0374
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10727 phoA

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4217
DrugBankiDB03498 Mercaptomethyl Phosphonate
DB02823 Phosphonoacetic Acid
DB04522 Phosphonoserine
DB04031 Serine Vanadate

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 21Add BLAST21
ChainiPRO_000002401222 – 471Alkaline phosphataseAdd BLAST450

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi190 ↔ 200
Disulfide bondi308 ↔ 358

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP00634
PRIDEiP00634

Interactioni

Subunit structurei

Isozymes 1 and 3 are a dimer of identical chains, isozyme 2 is a dimer of heterogeneous chains, one of each of the subunits from isozymes 1 and 3.

Binary interactionsi

Protein-protein interaction databases

BioGridi4259827, 50 interactors
DIPiDIP-10496N
IntActiP00634, 7 interactors
STRINGi316385.ECDH10B_0340

Structurei

Secondary structure

1471
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00634
SMRiP00634
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00634

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105G6Z Bacteria
COG1785 LUCA
HOGENOMiHOG000099117
InParanoidiP00634
KOiK01077
OMAiEHTGTQL
PhylomeDBiP00634

Family and domain databases

CDDicd16012 ALP, 1 hit
Gene3Di3.40.720.10, 1 hit
InterProiView protein in InterPro
IPR017849 Alkaline_Pase-like_a/b/a
IPR001952 Alkaline_phosphatase
IPR018299 Alkaline_phosphatase_AS
IPR017850 Alkaline_phosphatase_core_sf
PANTHERiPTHR11596 PTHR11596, 2 hits
PfamiView protein in Pfam
PF00245 Alk_phosphatase, 1 hit
PRINTSiPR00113 ALKPHPHTASE
SMARTiView protein in SMART
SM00098 alkPPc, 1 hit
SUPFAMiSSF53649 SSF53649, 1 hit
PROSITEiView protein in PROSITE
PS00123 ALKALINE_PHOSPHATASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00634-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKQSTIALAL LPLLFTPVTK ARTPEMPVLE NRAAQGDITA PGGARRLTGD
60 70 80 90 100
QTAALRDSLS DKPAKNIILL IGDGMGDSEI TAARNYAEGA GGFFKGIDAL
110 120 130 140 150
PLTGQYTHYA LNKKTGKPDY VTDSAASATA WSTGVKTYNG ALGVDIHEKD
160 170 180 190 200
HPTILEMAKA AGLATGNVST AELQDATPAA LVAHVTSRKC YGPSATSEKC
210 220 230 240 250
PGNALEKGGK GSITEQLLNA RADVTLGGGA KTFAETATAG EWQGKTLREQ
260 270 280 290 300
AQARGYQLVS DAASLNSVTE ANQQKPLLGL FADGNMPVRW LGPKATYHGN
310 320 330 340 350
IDKPAVTCTP NPQRNDSVPT LAQMTDKAIE LLSKNEKGFF LQVEGASIDK
360 370 380 390 400
QDHAANPCGQ IGETVDLDEA VQRALEFAKK EGNTLVIVTA DHAHASQIVA
410 420 430 440 450
PDTKAPGLTQ ALNTKDGAVM VMSYGNSEED SQEHTGSQLR IAAYGPHAAN
460 470
VVGLTDQTDL FYTMKAALGL K
Length:471
Mass (Da):49,439
Last modified:August 13, 1987 - v1
Checksum:i8A8DE1F29D9D9253
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10L → V in AAA23431 (PubMed:3912261).Curated1
Sequence conflicti78 – 80SEI → WGS in AAA24359 (PubMed:6273802).Curated3
Sequence conflicti198E → Q AA sequence (PubMed:7022451).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti22Missing in isozyme 3. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04586 Genomic DNA Translation: CAA28257.1
M13345 Genomic DNA Translation: AAA83893.1
M29664 Genomic DNA Translation: AAA24364.1
M29665 Genomic DNA Translation: AAA24365.1
U73857 Genomic DNA Translation: AAB18107.1
U00096 Genomic DNA Translation: AAC73486.2
AP009048 Genomic DNA Translation: BAE76164.1
M33536 Genomic DNA Translation: AAA24372.1
J01659 Genomic DNA Translation: AAA24359.2
J01660 Genomic DNA Translation: AAA24360.1
J01661 Genomic DNA Translation: AAA24361.1
J05005 Genomic DNA Translation: AAA24362.1
M14399 mRNA Translation: AAA23431.1
M13763 Genomic DNA Translation: AAA24358.1
PIRiA00776 PAECA
RefSeqiNP_414917.2, NC_000913.3
WP_000814403.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73486; AAC73486; b0383
BAE76164; BAE76164; BAE76164
GeneIDi945041
KEGGiecj:JW0374
eco:b0383
PATRICifig|1411691.4.peg.1895

Similar proteinsi

Entry informationi

Entry nameiPPB_ECOLI
AccessioniPrimary (citable) accession number: P00634
Secondary accession number(s): P77801
, P78051, Q2MC42, Q47041
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: August 13, 1987
Last modified: March 28, 2018
This is version 191 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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