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Protein

Basic phospholipase A2 notexin

Gene
N/A
Organism
Notechis scutatus scutatus (Mainland tiger snake) (Common tiger snake)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Snake venom phospholipase A2 (PLA2) that inhibits neuromuscular transmission by blocking acetylcholine release from the nerve termini. Is directly toxic to skeletal muscle upon local application in vivo (dystrophic effect). Also has direct nephrotoxicity in experimental mice; a single subcutaneous dose (1.38 µg/kg) produces renal tubular and glomerular damage within 24 hours (PubMed:7580101). PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.

Miscellaneous

Activity and lethal neurotoxicity are lost upon modification with p-bromophenacyl bromide.

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28Calcium; via carbonyl oxygenBy similarity1
Metal bindingi30Calcium; via carbonyl oxygenBy similarity1
Metal bindingi32Calcium; via carbonyl oxygenBy similarity1
Active sitei481 Publication1
Metal bindingi49CalciumBy similarity1
Active sitei931 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Myotoxin, Neurotoxin, Presynaptic neurotoxin, Toxin
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Basic phospholipase A2 notexin1 Publication (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
Phosphatidylcholine 2-acylhydrolase
OrganismiNotechis scutatus scutatus (Mainland tiger snake) (Common tiger snake)
Taxonomic identifieri70142 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeAcanthophiinaeNotechis

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

LD50 is 0.025 mg/kg by intravenous injection.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001616751 – 119Basic phospholipase A2 notexinAdd BLAST119

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi11 ↔ 71Combined sources1 Publication
Disulfide bondi27 ↔ 118Combined sources1 Publication
Disulfide bondi29 ↔ 45Combined sources1 Publication
Disulfide bondi44 ↔ 99Combined sources1 Publication
Disulfide bondi51 ↔ 92Combined sources1 Publication
Disulfide bondi60 ↔ 85Combined sources1 Publication
Disulfide bondi78 ↔ 90Combined sources1 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Monomer.

Structurei

Secondary structure

1119
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00608
SMRiP00608
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00608

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG008137

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit

Sequencei

Sequence statusi: Complete.

P00608-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
NLVQFSYLIQ CANHGKRPTW HYMDYGCYCG AGGSGTPVDE LDRCCKIHDD
60 70 80 90 100
CYDEAGKKGC FPKMSAYDYY CGENGPYCRN IKKKCLRFVC DCDVEAAFCF
110
AKAPYNNANW NIDTKKRCQ
Length:119
Mass (Da):13,593
Last modified:July 21, 1986 - v1
Checksum:i23D8BF780221D852
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti16K → R in variant S. 1

Sequence databases

PIRiA00749 PSNOAT
S08258 PSNOAS

Similar proteinsi

Cross-referencesi

Sequence databases

PIRiA00749 PSNOAT
S08258 PSNOAS

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AE7X-ray2.00A1-119[»]
4E4CX-ray1.80A1-119[»]
ProteinModelPortaliP00608
SMRiP00608
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137

Miscellaneous databases

EvolutionaryTraceiP00608

Family and domain databases

CDDicd00125 PLA2c, 1 hit
Gene3Di1.20.90.10, 1 hit
InterProiView protein in InterPro
IPR001211 PLipase_A2
IPR033112 PLipase_A2_Asp_AS
IPR016090 PLipase_A2_dom
IPR036444 PLipase_A2_dom_sf
IPR033113 PLipase_A2_His_AS
PANTHERiPTHR11716 PTHR11716, 1 hit
PfamiView protein in Pfam
PF00068 Phospholip_A2_1, 1 hit
PRINTSiPR00389 PHPHLIPASEA2
SMARTiView protein in SMART
SM00085 PA2c, 1 hit
SUPFAMiSSF48619 SSF48619, 1 hit
PROSITEiView protein in PROSITE
PS00119 PA2_ASP, 1 hit
PS00118 PA2_HIS, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPA2B_NOTSC
AccessioniPrimary (citable) accession number: P00608
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 22, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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