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Protein

DNA polymerase I

Gene

polA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

GO - Molecular functioni

GO - Biological processi

  • base-excision repair Source: EcoCyc
  • DNA-dependent DNA replication Source: EcoCyc
  • DNA repair Source: EcoCyc
  • DNA replication Source: EcoCyc

Keywordsi

Molecular functionDNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase
Biological processDNA damage, DNA repair, DNA replication

Enzyme and pathway databases

BioCyciEcoCyc:EG10746-MONOMER
MetaCyc:EG10746-MONOMER
BRENDAi2.7.7.7 2026
SABIO-RKiP00582

Names & Taxonomyi

Protein namesi
Recommended name:
DNA polymerase I (EC:2.7.7.7)
Short name:
POL I
Gene namesi
Name:polA
Synonyms:resA
Ordered Locus Names:b3863, JW3835
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG10746 polA

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4298
DrugBankiDB00548 Azelaic Acid
DB08432 THYMIDINE-5'-THIOPHOSPHATE

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001012391 – 928DNA polymerase IAdd BLAST928

Proteomic databases

EPDiP00582
PaxDbiP00582
PRIDEiP00582

2D gel databases

SWISS-2DPAGEiP00582

Interactioni

Subunit structurei

Single-chain monomer with multiple functions.

Protein-protein interaction databases

BioGridi4262182, 125 interactors
DIPiDIP-10524N
IntActiP00582, 33 interactors
STRINGi316385.ECDH10B_4052

Chemistry databases

BindingDBiP00582

Structurei

Secondary structure

1928
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00582
SMRiP00582
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00582

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 3235'-3' exonucleaseAdd BLAST323
Domaini324 – 5173'-5' exonucleaseAdd BLAST194

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni324 – 928Klenow fragmentAdd BLAST605
Regioni521 – 928PolymeraseAdd BLAST408

Sequence similaritiesi

Belongs to the DNA polymerase type-A family.Curated

Phylogenomic databases

eggNOGiENOG4105C2M Bacteria
COG0258 LUCA
COG0749 LUCA
HOGENOMiHOG000020998
InParanoidiP00582
KOiK02335
PhylomeDBiP00582

Family and domain databases

CDDicd09898 H3TH_53EXO, 1 hit
Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR002562 3'-5'_exonuclease_dom
IPR020046 5-3_exonucl_a-hlix_arch_N
IPR036279 5-3_exonuclease_C_sf
IPR002421 5-3_exonuclease_N
IPR019760 DNA-dir_DNA_pol_A_CS
IPR001098 DNA-dir_DNA_pol_A_palm_dom
IPR020045 DNA_polI_H3TH
IPR018320 DNA_polymerase_1
IPR002298 DNA_polymerase_A
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10133 PTHR10133, 1 hit
PfamiView protein in Pfam
PF01367 5_3_exonuc, 1 hit
PF02739 5_3_exonuc_N, 1 hit
PF00476 DNA_pol_A, 1 hit
PF01612 DNA_pol_A_exo1, 1 hit
PRINTSiPR00868 DNAPOLI
SMARTiView protein in SMART
SM00474 35EXOc, 1 hit
SM00475 53EXOc, 1 hit
SM00279 HhH2, 1 hit
SM00482 POLAc, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF53098 SSF53098, 1 hit
SSF88723 SSF88723, 1 hit
TIGRFAMsiTIGR00593 pola, 1 hit
PROSITEiView protein in PROSITE
PS00447 DNA_POLYMERASE_A, 1 hit

Sequencei

Sequence statusi: Complete.

P00582-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVQIPQNPLI LVDGSSYLYR AYHAFPPLTN SAGEPTGAMY GVLNMLRSLI
60 70 80 90 100
MQYKPTHAAV VFDAKGKTFR DELFEHYKSH RPPMPDDLRA QIEPLHAMVK
110 120 130 140 150
AMGLPLLAVS GVEADDVIGT LAREAEKAGR PVLISTGDKD MAQLVTPNIT
160 170 180 190 200
LINTMTNTIL GPEEVVNKYG VPPELIIDFL ALMGDSSDNI PGVPGVGEKT
210 220 230 240 250
AQALLQGLGG LDTLYAEPEK IAGLSFRGAK TMAAKLEQNK EVAYLSYQLA
260 270 280 290 300
TIKTDVELEL TCEQLEVQQP AAEELLGLFK KYEFKRWTAD VEAGKWLQAK
310 320 330 340 350
GAKPAAKPQE TSVADEAPEV TATVISYDNY VTILDEETLK AWIAKLEKAP
360 370 380 390 400
VFAFDTETDS LDNISANLVG LSFAIEPGVA AYIPVAHDYL DAPDQISRER
410 420 430 440 450
ALELLKPLLE DEKALKVGQN LKYDRGILAN YGIELRGIAF DTMLESYILN
460 470 480 490 500
SVAGRHDMDS LAERWLKHKT ITFEEIAGKG KNQLTFNQIA LEEAGRYAAE
510 520 530 540 550
DADVTLQLHL KMWPDLQKHK GPLNVFENIE MPLVPVLSRI ERNGVKIDPK
560 570 580 590 600
VLHNHSEELT LRLAELEKKA HEIAGEEFNL SSTKQLQTIL FEKQGIKPLK
610 620 630 640 650
KTPGGAPSTS EEVLEELALD YPLPKVILEY RGLAKLKSTY TDKLPLMINP
660 670 680 690 700
KTGRVHTSYH QAVTATGRLS STDPNLQNIP VRNEEGRRIR QAFIAPEDYV
710 720 730 740 750
IVSADYSQIE LRIMAHLSRD KGLLTAFAEG KDIHRATAAE VFGLPLETVT
760 770 780 790 800
SEQRRSAKAI NFGLIYGMSA FGLARQLNIP RKEAQKYMDL YFERYPGVLE
810 820 830 840 850
YMERTRAQAK EQGYVETLDG RRLYLPDIKS SNGARRAAAE RAAINAPMQG
860 870 880 890 900
TAADIIKRAM IAVDAWLQAE QPRVRMIMQV HDELVFEVHK DDVDAVAKQI
910 920
HQLMENCTRL DVPLLVEVGS GENWDQAH
Length:928
Mass (Da):103,118
Last modified:July 21, 1986 - v1
Checksum:iDAAE1C448A59030C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00317 Genomic DNA Translation: CAA23607.1
L19201 Genomic DNA Translation: AAB02998.1
U00096 Genomic DNA Translation: AAC76861.1
AP009048 Genomic DNA Translation: BAE77445.1
J01663 Genomic DNA Translation: AAA24402.1
J01664 Genomic DNA Translation: AAA24404.1
PIRiA92360 DJECI
RefSeqiNP_418300.1, NC_000913.3
WP_000250006.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC76861; AAC76861; b3863
BAE77445; BAE77445; BAE77445
GeneIDi948356
KEGGiecj:JW3835
eco:b3863
PATRICifig|1411691.4.peg.2851

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00317 Genomic DNA Translation: CAA23607.1
L19201 Genomic DNA Translation: AAB02998.1
U00096 Genomic DNA Translation: AAC76861.1
AP009048 Genomic DNA Translation: BAE77445.1
J01663 Genomic DNA Translation: AAA24402.1
J01664 Genomic DNA Translation: AAA24404.1
PIRiA92360 DJECI
RefSeqiNP_418300.1, NC_000913.3
WP_000250006.1, NZ_LN832404.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1D8YX-ray2.08A324-928[»]
1D9DX-ray2.18A324-928[»]
1D9FX-ray3.00A324-928[»]
1DPIX-ray2.80A324-928[»]
1KFDX-ray3.90A324-928[»]
1KFSX-ray2.10A324-928[»]
1KLNX-ray3.20A324-928[»]
1KRPX-ray2.20A324-928[»]
1KSPX-ray2.30A324-928[»]
1QSLX-ray2.20A324-928[»]
2KFNX-ray2.03A324-928[»]
2KFZX-ray2.03A324-928[»]
2KZMX-ray2.60A324-928[»]
2KZZX-ray2.25A324-928[»]
ProteinModelPortaliP00582
SMRiP00582
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4262182, 125 interactors
DIPiDIP-10524N
IntActiP00582, 33 interactors
STRINGi316385.ECDH10B_4052

Chemistry databases

BindingDBiP00582
ChEMBLiCHEMBL4298
DrugBankiDB00548 Azelaic Acid
DB08432 THYMIDINE-5'-THIOPHOSPHATE

2D gel databases

SWISS-2DPAGEiP00582

Proteomic databases

EPDiP00582
PaxDbiP00582
PRIDEiP00582

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC76861; AAC76861; b3863
BAE77445; BAE77445; BAE77445
GeneIDi948356
KEGGiecj:JW3835
eco:b3863
PATRICifig|1411691.4.peg.2851

Organism-specific databases

EchoBASEiEB0739
EcoGeneiEG10746 polA

Phylogenomic databases

eggNOGiENOG4105C2M Bacteria
COG0258 LUCA
COG0749 LUCA
HOGENOMiHOG000020998
InParanoidiP00582
KOiK02335
PhylomeDBiP00582

Enzyme and pathway databases

BioCyciEcoCyc:EG10746-MONOMER
MetaCyc:EG10746-MONOMER
BRENDAi2.7.7.7 2026
SABIO-RKiP00582

Miscellaneous databases

EvolutionaryTraceiP00582
PROiPR:P00582

Family and domain databases

CDDicd09898 H3TH_53EXO, 1 hit
Gene3Di3.30.420.10, 1 hit
InterProiView protein in InterPro
IPR002562 3'-5'_exonuclease_dom
IPR020046 5-3_exonucl_a-hlix_arch_N
IPR036279 5-3_exonuclease_C_sf
IPR002421 5-3_exonuclease_N
IPR019760 DNA-dir_DNA_pol_A_CS
IPR001098 DNA-dir_DNA_pol_A_palm_dom
IPR020045 DNA_polI_H3TH
IPR018320 DNA_polymerase_1
IPR002298 DNA_polymerase_A
IPR008918 HhH2
IPR029060 PIN-like_dom_sf
IPR012337 RNaseH-like_sf
IPR036397 RNaseH_sf
PANTHERiPTHR10133 PTHR10133, 1 hit
PfamiView protein in Pfam
PF01367 5_3_exonuc, 1 hit
PF02739 5_3_exonuc_N, 1 hit
PF00476 DNA_pol_A, 1 hit
PF01612 DNA_pol_A_exo1, 1 hit
PRINTSiPR00868 DNAPOLI
SMARTiView protein in SMART
SM00474 35EXOc, 1 hit
SM00475 53EXOc, 1 hit
SM00279 HhH2, 1 hit
SM00482 POLAc, 1 hit
SUPFAMiSSF47807 SSF47807, 1 hit
SSF53098 SSF53098, 1 hit
SSF88723 SSF88723, 1 hit
TIGRFAMsiTIGR00593 pola, 1 hit
PROSITEiView protein in PROSITE
PS00447 DNA_POLYMERASE_A, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDPO1_ECOLI
AccessioniPrimary (citable) accession number: P00582
Secondary accession number(s): Q2M8G1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 7, 2018
This is version 192 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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