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UniProtKB - P00582 (DPO1_ECOLI)
Protein
DNA polymerase I
Gene
polA
Organism
Escherichia coli (strain K12)
Status
Functioni
In addition to polymerase activity, this DNA polymerase exhibits 3'-5' and 5'-3' exonuclease activity. It is able to utilize nicked circular duplex DNA as a template and can unwind the parental DNA strand from its template.
Catalytic activityi
- EC:2.7.7.7
GO - Molecular functioni
- 3'-5' exonuclease activity Source: EcoCyc
- 5'-3' exonuclease activity Source: EcoCyc
- DNA binding Source: EcoCyc
- DNA-directed DNA polymerase activity Source: EcoCyc
GO - Biological processi
- base-excision repair Source: EcoCyc
- DNA-dependent DNA replication Source: EcoCyc
- DNA repair Source: EcoCyc
- DNA replication Source: EcoCyc
- double-strand break repair Source: GO_Central
Keywordsi
Molecular function | DNA-binding, DNA-directed DNA polymerase, Exonuclease, Hydrolase, Nuclease, Nucleotidyltransferase, Transferase |
Biological process | DNA damage, DNA repair, DNA replication |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10746-MONOMER |
BRENDAi | 2.7.7.7, 2026 |
SABIO-RKi | P00582 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:polA Synonyms:resA Ordered Locus Names:b3863, JW3835 |
Organismi | Escherichia coli (strain K12) |
Taxonomic identifieri | 83333 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia › |
Proteomesi |
|
Subcellular locationi
Pathology & Biotechi
Chemistry databases
ChEMBLi | CHEMBL4298 |
DrugBanki | DB00548, Azelaic acid DB08432, THYMIDINE-5'-THIOPHOSPHATE |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101239 | 1 – 928 | DNA polymerase IAdd BLAST | 928 |
Proteomic databases
jPOSTi | P00582 |
PaxDbi | P00582 |
PRIDEi | P00582 |
2D gel databases
SWISS-2DPAGEi | P00582 |
Interactioni
Subunit structurei
Single-chain monomer with multiple functions.
Protein-protein interaction databases
BioGRIDi | 4262182, 125 interactors |
DIPi | DIP-10524N |
IntActi | P00582, 33 interactors |
STRINGi | 511145.b3863 |
Chemistry databases
BindingDBi | P00582 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00582 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00582 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 323 | 5'-3' exonucleaseAdd BLAST | 323 | |
Domaini | 324 – 517 | 3'-5' exonucleaseAdd BLAST | 194 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 324 – 928 | Klenow fragmentAdd BLAST | 605 | |
Regioni | 521 – 928 | PolymeraseAdd BLAST | 408 |
Sequence similaritiesi
Belongs to the DNA polymerase type-A family.Curated
Phylogenomic databases
eggNOGi | COG0258, Bacteria COG0749, Bacteria |
HOGENOMi | CLU_004675_0_1_6 |
InParanoidi | P00582 |
PhylomeDBi | P00582 |
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 1 hit |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit PF01612, DNA_pol_A_exo1, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00475, 53EXOc, 1 hit SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
i Sequence
Sequence statusi: Complete.
P00582-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVQIPQNPLI LVDGSSYLYR AYHAFPPLTN SAGEPTGAMY GVLNMLRSLI
60 70 80 90 100
MQYKPTHAAV VFDAKGKTFR DELFEHYKSH RPPMPDDLRA QIEPLHAMVK
110 120 130 140 150
AMGLPLLAVS GVEADDVIGT LAREAEKAGR PVLISTGDKD MAQLVTPNIT
160 170 180 190 200
LINTMTNTIL GPEEVVNKYG VPPELIIDFL ALMGDSSDNI PGVPGVGEKT
210 220 230 240 250
AQALLQGLGG LDTLYAEPEK IAGLSFRGAK TMAAKLEQNK EVAYLSYQLA
260 270 280 290 300
TIKTDVELEL TCEQLEVQQP AAEELLGLFK KYEFKRWTAD VEAGKWLQAK
310 320 330 340 350
GAKPAAKPQE TSVADEAPEV TATVISYDNY VTILDEETLK AWIAKLEKAP
360 370 380 390 400
VFAFDTETDS LDNISANLVG LSFAIEPGVA AYIPVAHDYL DAPDQISRER
410 420 430 440 450
ALELLKPLLE DEKALKVGQN LKYDRGILAN YGIELRGIAF DTMLESYILN
460 470 480 490 500
SVAGRHDMDS LAERWLKHKT ITFEEIAGKG KNQLTFNQIA LEEAGRYAAE
510 520 530 540 550
DADVTLQLHL KMWPDLQKHK GPLNVFENIE MPLVPVLSRI ERNGVKIDPK
560 570 580 590 600
VLHNHSEELT LRLAELEKKA HEIAGEEFNL SSTKQLQTIL FEKQGIKPLK
610 620 630 640 650
KTPGGAPSTS EEVLEELALD YPLPKVILEY RGLAKLKSTY TDKLPLMINP
660 670 680 690 700
KTGRVHTSYH QAVTATGRLS STDPNLQNIP VRNEEGRRIR QAFIAPEDYV
710 720 730 740 750
IVSADYSQIE LRIMAHLSRD KGLLTAFAEG KDIHRATAAE VFGLPLETVT
760 770 780 790 800
SEQRRSAKAI NFGLIYGMSA FGLARQLNIP RKEAQKYMDL YFERYPGVLE
810 820 830 840 850
YMERTRAQAK EQGYVETLDG RRLYLPDIKS SNGARRAAAE RAAINAPMQG
860 870 880 890 900
TAADIIKRAM IAVDAWLQAE QPRVRMIMQV HDELVFEVHK DDVDAVAKQI
910 920
HQLMENCTRL DVPLLVEVGS GENWDQAH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00317 Genomic DNA Translation: CAA23607.1 L19201 Genomic DNA Translation: AAB02998.1 U00096 Genomic DNA Translation: AAC76861.1 AP009048 Genomic DNA Translation: BAE77445.1 J01663 Genomic DNA Translation: AAA24402.1 J01664 Genomic DNA Translation: AAA24404.1 |
PIRi | A92360, DJECI |
RefSeqi | NP_418300.1, NC_000913.3 WP_000250006.1, NZ_SSZK01000026.1 |
Genome annotation databases
EnsemblBacteriai | AAC76861; AAC76861; b3863 BAE77445; BAE77445; BAE77445 |
GeneIDi | 66672231 948356 |
KEGGi | ecj:JW3835 eco:b3863 |
PATRICi | fig|1411691.4.peg.2851 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00317 Genomic DNA Translation: CAA23607.1 L19201 Genomic DNA Translation: AAB02998.1 U00096 Genomic DNA Translation: AAC76861.1 AP009048 Genomic DNA Translation: BAE77445.1 J01663 Genomic DNA Translation: AAA24402.1 J01664 Genomic DNA Translation: AAA24404.1 |
PIRi | A92360, DJECI |
RefSeqi | NP_418300.1, NC_000913.3 WP_000250006.1, NZ_SSZK01000026.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1D8Y | X-ray | 2.08 | A | 324-928 | [»] | |
1D9D | X-ray | 2.18 | A | 324-928 | [»] | |
1D9F | X-ray | 3.00 | A | 324-928 | [»] | |
1DPI | X-ray | 2.80 | A | 324-928 | [»] | |
1KFD | X-ray | 3.90 | A | 324-928 | [»] | |
1KFS | X-ray | 2.10 | A | 324-928 | [»] | |
1KLN | X-ray | 3.20 | A | 324-928 | [»] | |
1KRP | X-ray | 2.20 | A | 324-928 | [»] | |
1KSP | X-ray | 2.30 | A | 324-928 | [»] | |
1QSL | X-ray | 2.20 | A | 324-928 | [»] | |
2KFN | X-ray | 2.03 | A | 324-928 | [»] | |
2KFZ | X-ray | 2.03 | A | 324-928 | [»] | |
2KZM | X-ray | 2.60 | A | 324-928 | [»] | |
2KZZ | X-ray | 2.25 | A | 324-928 | [»] | |
SMRi | P00582 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 4262182, 125 interactors |
DIPi | DIP-10524N |
IntActi | P00582, 33 interactors |
STRINGi | 511145.b3863 |
Chemistry databases
BindingDBi | P00582 |
ChEMBLi | CHEMBL4298 |
DrugBanki | DB00548, Azelaic acid DB08432, THYMIDINE-5'-THIOPHOSPHATE |
2D gel databases
SWISS-2DPAGEi | P00582 |
Proteomic databases
jPOSTi | P00582 |
PaxDbi | P00582 |
PRIDEi | P00582 |
Genome annotation databases
EnsemblBacteriai | AAC76861; AAC76861; b3863 BAE77445; BAE77445; BAE77445 |
GeneIDi | 66672231 948356 |
KEGGi | ecj:JW3835 eco:b3863 |
PATRICi | fig|1411691.4.peg.2851 |
Organism-specific databases
EchoBASEi | EB0739 |
Phylogenomic databases
eggNOGi | COG0258, Bacteria COG0749, Bacteria |
HOGENOMi | CLU_004675_0_1_6 |
InParanoidi | P00582 |
PhylomeDBi | P00582 |
Enzyme and pathway databases
BioCyci | EcoCyc:EG10746-MONOMER |
BRENDAi | 2.7.7.7, 2026 |
SABIO-RKi | P00582 |
Miscellaneous databases
EvolutionaryTracei | P00582 |
PROi | PR:P00582 |
Family and domain databases
CDDi | cd09898, H3TH_53EXO, 1 hit |
Gene3Di | 3.30.420.10, 1 hit |
InterProi | View protein in InterPro IPR002562, 3'-5'_exonuclease_dom IPR020046, 5-3_exonucl_a-hlix_arch_N IPR002421, 5-3_exonuclease IPR036279, 5-3_exonuclease_C_sf IPR019760, DNA-dir_DNA_pol_A_CS IPR001098, DNA-dir_DNA_pol_A_palm_dom IPR043502, DNA/RNA_pol_sf IPR020045, DNA_polI_H3TH IPR018320, DNA_polymerase_1 IPR002298, DNA_polymerase_A IPR008918, HhH2 IPR029060, PIN-like_dom_sf IPR012337, RNaseH-like_sf IPR036397, RNaseH_sf |
PANTHERi | PTHR10133, PTHR10133, 1 hit |
Pfami | View protein in Pfam PF01367, 5_3_exonuc, 1 hit PF02739, 5_3_exonuc_N, 1 hit PF00476, DNA_pol_A, 1 hit PF01612, DNA_pol_A_exo1, 1 hit |
PRINTSi | PR00868, DNAPOLI |
SMARTi | View protein in SMART SM00474, 35EXOc, 1 hit SM00475, 53EXOc, 1 hit SM00279, HhH2, 1 hit SM00482, POLAc, 1 hit |
SUPFAMi | SSF47807, SSF47807, 1 hit SSF53098, SSF53098, 1 hit SSF56672, SSF56672, 1 hit SSF88723, SSF88723, 1 hit |
TIGRFAMsi | TIGR00593, pola, 1 hit |
PROSITEi | View protein in PROSITE PS00447, DNA_POLYMERASE_A, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DPO1_ECOLI | |
Accessioni | P00582Primary (citable) accession number: P00582 Secondary accession number(s): Q2M8G1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 211 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families