UniProtKB - P00573 (RPOL_BPT7)
Protein
T7 RNA polymerase
Gene
1
Organism
Escherichia phage T7 (Bacteriophage T7)
Status
Functioni
Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an 'abortive phase' where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Plays also a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process.5 Publications
Catalytic activityi
- a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1)PROSITE-ProRule annotation1 PublicationEC:2.7.7.6PROSITE-ProRule annotation1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 537 | 1 Publication | 1 | |
Active sitei | 631 | 2 Publications | 1 | |
Active sitei | 812 | 1 Publication | 1 |
GO - Molecular functioni
- DNA binding Source: InterPro
- DNA-directed 5'-3' RNA polymerase activity Source: UniProtKB-KW
GO - Biological processi
- DNA-templated viral transcription Source: UniProtKB
- transcription, DNA-templated Source: InterPro
Keywordsi
Molecular function | Nucleotidyltransferase, Transferase |
Biological process | Transcription, Viral transcription |
Enzyme and pathway databases
BRENDAi | 2.7.7.6, 736 |
Names & Taxonomyi
Protein namesi | Recommended name: T7 RNA polymeraseAlternative name(s): DNA-directed RNA polymerase (EC:2.7.7.61 Publication) |
Gene namesi | Ordered Locus Names:1 |
Organismi | Escherichia phage T7 (Bacteriophage T7) |
Taxonomic identifieri | 10760 [NCBI] |
Taxonomic lineagei | Viruses › Duplodnaviria › Heunggongvirae › Uroviricota › Caudoviricetes › Caudovirales › Autographiviridae › Studiervirinae › Teseptimavirus › |
Virus hosti | Escherichia coli [TaxID: 562] |
Proteomesi |
|
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 172 | K → L or G: No change in activity. 1 Publication | 1 | |
Mutagenesisi | 563 | P → A or T: Inactivated. | 1 | |
Mutagenesisi | 571 | Y → S: Inactivated. | 1 | |
Mutagenesisi | 631 | K → G: Partially inactivated. 1 Publication | 1 | |
Mutagenesisi | 631 | K → L: Partially inactivated. 1 Publication | 1 | |
Mutagenesisi | 631 | K → R: Partially inactivated. 1 Publication | 1 | |
Mutagenesisi | 636 | T → P: Inactivated. | 1 | |
Mutagenesisi | 639 | Y → D: Inactivated. | 1 | |
Mutagenesisi | 646 | F → C: Inactivated. | 1 |
Chemistry databases
ChEMBLi | CHEMBL1075072 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087749 | 1 – 883 | T7 RNA polymeraseAdd BLAST | 883 |
Interactioni
Subunit structurei
Monomer.
Interacts with T7 lysozyme; this interaction inhibits transcriptional function of T7 RNA polymerase.
Protein-protein interaction databases
DIPi | DIP-6091N |
IntActi | P00573, 1 interactor |
MINTi | P00573 |
Chemistry databases
BindingDBi | P00573 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00573 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00573 |
Family & Domainsi
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 380 – 383 | Poly-Ala | 4 |
Sequence similaritiesi
Belongs to the phage and mitochondrial RNA polymerase family.Curated
Family and domain databases
Gene3Di | 1.10.1320.10, 1 hit 1.10.287.260, 1 hit |
InterProi | View protein in InterPro IPR024075, DNA-dir_RNA_pol_helix_hairp_sf IPR002092, DNA-dir_Rpol_phage-type IPR043502, DNA/RNA_pol_sf IPR037159, RNA_POL_N_sf IPR029262, RPOL_N |
PANTHERi | PTHR10102, PTHR10102, 1 hit |
Pfami | View protein in Pfam PF00940, RNA_pol, 1 hit PF14700, RPOL_N, 1 hit |
SMARTi | View protein in SMART SM01311, RPOL_N, 1 hit |
SUPFAMi | SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00900, RNA_POL_PHAGE_1, 1 hit PS00489, RNA_POL_PHAGE_2, 1 hit |
i Sequence
Sequence statusi: Complete.
P00573-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNTINIAKND FSDIELAAIP FNTLADHYGE RLAREQLALE HESYEMGEAR
60 70 80 90 100
FRKMFERQLK AGEVADNAAA KPLITTLLPK MIARINDWFE EVKAKRGKRP
110 120 130 140 150
TAFQFLQEIK PEAVAYITIK TTLACLTSAD NTTVQAVASA IGRAIEDEAR
160 170 180 190 200
FGRIRDLEAK HFKKNVEEQL NKRVGHVYKK AFMQVVEADM LSKGLLGGEA
210 220 230 240 250
WSSWHKEDSI HVGVRCIEML IESTGMVSLH RQNAGVVGQD SETIELAPEY
260 270 280 290 300
AEAIATRAGA LAGISPMFQP CVVPPKPWTG ITGGGYWANG RRPLALVRTH
310 320 330 340 350
SKKALMRYED VYMPEVYKAI NIAQNTAWKI NKKVLAVANV ITKWKHCPVE
360 370 380 390 400
DIPAIEREEL PMKPEDIDMN PEALTAWKRA AAAVYRKDKA RKSRRISLEF
410 420 430 440 450
MLEQANKFAN HKAIWFPYNM DWRGRVYAVS MFNPQGNDMT KGLLTLAKGK
460 470 480 490 500
PIGKEGYYWL KIHGANCAGV DKVPFPERIK FIEENHENIM ACAKSPLENT
510 520 530 540 550
WWAEQDSPFC FLAFCFEYAG VQHHGLSYNC SLPLAFDGSC SGIQHFSAML
560 570 580 590 600
RDEVGGRAVN LLPSETVQDI YGIVAKKVNE ILQADAINGT DNEVVTVTDE
610 620 630 640 650
NTGEISEKVK LGTKALAGQW LAYGVTRSVT KRSVMTLAYG SKEFGFRQQV
660 670 680 690 700
LEDTIQPAID SGKGLMFTQP NQAAGYMAKL IWESVSVTVV AAVEAMNWLK
710 720 730 740 750
SAAKLLAAEV KDKKTGEILR KRCAVHWVTP DGFPVWQEYK KPIQTRLNLM
760 770 780 790 800
FLGQFRLQPT INTNKDSEID AHKQESGIAP NFVHSQDGSH LRKTVVWAHE
810 820 830 840 850
KYGIESFALI HDSFGTIPAD AANLFKAVRE TMVDTYESCD VLADFYDQFA
860 870 880
DQLHESQLDK MPALPAKGNL NLRDILESDF AFA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 388 – 424 | DKARK…MDWRG → TRLASLAVSALSSCLSKPIS LLTIRPSGSLTTWTGAV in CAA24333 (PubMed:7310873).CuratedAdd BLAST | 37 | |
Sequence conflicti | 389 | K → R in AAA32569 (PubMed:6093820).Curated | 1 | |
Sequence conflicti | 443 | L → R in CAA24333 (PubMed:7310873).Curated | 1 | |
Sequence conflicti | 474 | P → S in CAA24333 (PubMed:7310873).Curated | 1 | |
Sequence conflicti | 623 | Y → H in AAA32569 (PubMed:6093820).Curated | 1 | |
Sequence conflicti | 665 | L → P in AAA32569 (PubMed:6093820).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01146 Genomic DNA Translation: CAA24390.1 V01127 Genomic DNA Translation: CAA24333.1 M38308 Genomic DNA Translation: AAA32569.1 |
PIRi | A94615, RNBP17 |
RefSeqi | NP_041960.1, NC_001604.1 |
Genome annotation databases
GeneIDi | 1261050 |
KEGGi | vg:1261050 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01146 Genomic DNA Translation: CAA24390.1 V01127 Genomic DNA Translation: CAA24333.1 M38308 Genomic DNA Translation: AAA32569.1 |
PIRi | A94615, RNBP17 |
RefSeqi | NP_041960.1, NC_001604.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ARO | X-ray | 2.80 | P | 1-883 | [»] | |
1CEZ | X-ray | 2.40 | A | 1-883 | [»] | |
1H38 | X-ray | 2.90 | A/B/C/D | 1-883 | [»] | |
1MSW | X-ray | 2.10 | D | 1-883 | [»] | |
1QLN | X-ray | 2.40 | A | 1-883 | [»] | |
1S0V | X-ray | 3.20 | A/B/C/D | 1-883 | [»] | |
1S76 | X-ray | 2.88 | D | 1-883 | [»] | |
1S77 | X-ray | 2.69 | D | 1-883 | [»] | |
2PI4 | X-ray | 2.50 | A | 6-883 | [»] | |
2PI5 | X-ray | 2.90 | A | 6-883 | [»] | |
3E2E | X-ray | 3.00 | A | 1-883 | [»] | |
3E3J | X-ray | 6.70 | B/C | 1-883 | [»] | |
4RNP | X-ray | 3.00 | A/B/C | 1-883 | [»] | |
SMRi | P00573 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
DIPi | DIP-6091N |
IntActi | P00573, 1 interactor |
MINTi | P00573 |
Chemistry databases
BindingDBi | P00573 |
ChEMBLi | CHEMBL1075072 |
Genome annotation databases
GeneIDi | 1261050 |
KEGGi | vg:1261050 |
Enzyme and pathway databases
BRENDAi | 2.7.7.6, 736 |
Miscellaneous databases
EvolutionaryTracei | P00573 |
PROi | PR:P00573 |
Family and domain databases
Gene3Di | 1.10.1320.10, 1 hit 1.10.287.260, 1 hit |
InterProi | View protein in InterPro IPR024075, DNA-dir_RNA_pol_helix_hairp_sf IPR002092, DNA-dir_Rpol_phage-type IPR043502, DNA/RNA_pol_sf IPR037159, RNA_POL_N_sf IPR029262, RPOL_N |
PANTHERi | PTHR10102, PTHR10102, 1 hit |
Pfami | View protein in Pfam PF00940, RNA_pol, 1 hit PF14700, RPOL_N, 1 hit |
SMARTi | View protein in SMART SM01311, RPOL_N, 1 hit |
SUPFAMi | SSF56672, SSF56672, 1 hit |
PROSITEi | View protein in PROSITE PS00900, RNA_POL_PHAGE_1, 1 hit PS00489, RNA_POL_PHAGE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RPOL_BPT7 | |
Accessioni | P00573Primary (citable) accession number: P00573 Secondary accession number(s): Q38543 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 11, 2001 | |
Last modified: | April 7, 2021 | |
This is version 155 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Viral Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families