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UniProtKB - P00573 (RPOL_BPT7)

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Protein

T7 RNA polymerase

Gene

1

Organism
Enterobacteria phage T7 (Bacteriophage T7)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Highly processive DNA-dependent RNA polymerase that catalyzes the transcription of class II and class III viral genes. Recognizes a specific promoter sequence and enters first into an "abortive phase" where very short transcripts are synthesized and released before proceeding to the processive transcription of long RNA chains. Unwinds the double-stranded DNA to expose the coding strand for templating. Participates in the initiation of viral DNA replication presumably by making primers accessible to the DNA polymerase, thus facilitating the DNA opening. Plays also a role in viral DNA packaging, probably by pausing the transcription at the right end of concatemer junction to allow packaging complex recruitment and beginning of the packaging process.5 Publications

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei5371 Publication1
Active sitei6312 Publications1
Active sitei8121 Publication1

GO - Molecular functioni

View the complete GO annotation on QuickGO ...

GO - Biological processi

  • DNA-templated viral transcription Source: UniProtKB
  • transcription, DNA-templated Source: InterPro
View the complete GO annotation on QuickGO ...

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTranscription, Viral transcription

Enzyme and pathway databases

BRENDAi2.7.7.6 736

Names & Taxonomyi

Protein namesi
Recommended name:
T7 RNA polymerase
Alternative name(s):
DNA-directed RNA polymerase (EC:2.7.7.61 Publication)
Gene namesi
Ordered Locus Names:1
OrganismiEnterobacteria phage T7 (Bacteriophage T7)
Taxonomic identifieri10760 [NCBI]
Taxonomic lineageiViruses › dsDNA viruses, no RNA stage › Caudovirales › Podoviridae › Autographivirinae › T7virus ›
Virus hostiEscherichia coli [TaxID: 562]
Proteomesi
  • UP000000840 Componenti: Genome

Subcellular locationi

Keywords - Cellular componenti

DNA-directed RNA polymerase

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi172K → L or G: No change in activity. 1 Publication1
Mutagenesisi563P → A or T: Inactivated. 1
Mutagenesisi571Y → S: Inactivated. 1
Mutagenesisi631K → G: Partially inactivated. 1 Publication1
Mutagenesisi631K → L: Partially inactivated. 1 Publication1
Mutagenesisi631K → R: Partially inactivated. 1 Publication1
Mutagenesisi636T → P: Inactivated. 1
Mutagenesisi639Y → D: Inactivated. 1
Mutagenesisi646F → C: Inactivated. 1

Chemistry databases

ChEMBLiCHEMBL1075072

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000877491 – 883T7 RNA polymeraseAdd BLAST883

Proteomic databases

PRIDEiP00573

Miscellaneous databases

PMAP-CutDBiP00573

Interactioni

Subunit structurei

Monomer. Interacts with T7 lysozyme; this interaction inhibits transcriptional function of T7 RNA polymerase.

Protein-protein interaction databases

DIPiDIP-6091N
IntActiP00573, 1 interactor
MINTiP00573

Chemistry databases

BindingDBiP00573

Structurei

Secondary structure

1883
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 10Combined sources4
Helixi12 – 14Combined sources3
Helixi18 – 24Combined sources7
Turni25 – 28Combined sources4
Helixi30 – 59Combined sources30
Turni60 – 62Combined sources3
Helixi64 – 66Combined sources3
Turni68 – 70Combined sources3
Helixi71 – 94Combined sources24
Turni101 – 107Combined sources7
Helixi111 – 128Combined sources18
Beta strandi129 – 131Combined sources3
Helixi134 – 156Combined sources23
Helixi162 – 165Combined sources4
Helixi168 – 172Combined sources5
Helixi176 – 189Combined sources14
Turni191 – 198Combined sources8
Turni205 – 207Combined sources3
Helixi208 – 223Combined sources16
Beta strandi227 – 230Combined sources4
Beta strandi231 – 233Combined sources3
Turni237 – 239Combined sources3
Beta strandi243 – 246Combined sources4
Helixi248 – 261Combined sources14
Beta strandi271 – 273Combined sources3
Beta strandi279 – 283Combined sources5
Beta strandi285 – 288Combined sources4
Beta strandi289 – 291Combined sources3
Beta strandi295 – 298Combined sources4
Helixi304 – 307Combined sources4
Turni308 – 310Combined sources3
Helixi314 – 324Combined sources11
Beta strandi328 – 330Combined sources3
Helixi332 – 341Combined sources10
Beta strandi344 – 346Combined sources3
Beta strandi348 – 351Combined sources4
Beta strandi365 – 368Combined sources4
Turni370 – 372Combined sources3
Helixi374 – 377Combined sources4
Helixi379 – 402Combined sources24
Turni403 – 405Combined sources3
Beta strandi406 – 410Combined sources5
Beta strandi418 – 421Combined sources4
Beta strandi422 – 424Combined sources3
Beta strandi426 – 432Combined sources7
Beta strandi434 – 436Combined sources3
Helixi438 – 443Combined sources6
Beta strandi444 – 448Combined sources5
Helixi453 – 467Combined sources15
Helixi475 – 484Combined sources10
Helixi486 – 494Combined sources9
Helixi496 – 499Combined sources4
Helixi501 – 504Combined sources4
Beta strandi505 – 507Combined sources3
Helixi508 – 524Combined sources17
Helixi525 – 527Combined sources3
Beta strandi528 – 530Combined sources3
Beta strandi534 – 537Combined sources4
Helixi541 – 550Combined sources10
Helixi555 – 558Combined sources4
Beta strandi559 – 564Combined sources6
Helixi570 – 586Combined sources17
Beta strandi594 – 596Combined sources3
Beta strandi597 – 602Combined sources6
Beta strandi605 – 610Combined sources6
Helixi613 – 621Combined sources9
Helixi627 – 635Combined sources9
Helixi636 – 639Combined sources4
Helixi643 – 653Combined sources11
Helixi655 – 660Combined sources6
Turni662 – 666Combined sources5
Helixi670 – 688Combined sources19
Helixi690 – 707Combined sources18
Turni713 – 715Combined sources3
Beta strandi718 – 720Combined sources3
Beta strandi725 – 728Combined sources4
Turni730 – 732Combined sources3
Beta strandi734 – 737Combined sources4
Beta strandi740 – 746Combined sources7
Beta strandi747 – 749Combined sources3
Beta strandi750 – 752Combined sources3
Beta strandi755 – 757Combined sources3
Beta strandi760 – 769Combined sources10
Helixi771 – 801Combined sources31
Beta strandi807 – 809Combined sources3
Beta strandi811 – 816Combined sources6
Helixi818 – 820Combined sources3
Helixi821 – 838Combined sources18
Helixi841 – 849Combined sources9
Turni852 – 854Combined sources3
Turni855 – 858Combined sources4
Beta strandi859 – 861Combined sources3
Helixi872 – 876Combined sources5
Turni879 – 881Combined sources3

3D structure databases

ProteinModelPortaliP00573
SMRiP00573
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00573

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi380 – 383Poly-Ala4

Sequence similaritiesi

Belongs to the phage and mitochondrial RNA polymerase family.Curated

Phylogenomic databases

KOiK21313
OrthoDBiVOG090000ME

Family and domain databases

Gene3Di1.10.1320.10, 1 hit
1.10.287.260, 1 hit
InterProiView protein in InterPro
IPR024075 DNA-dir_RNA_pol_helix_hairp_sf
IPR002092 DNA-dir_Rpol_phage-type
IPR037159 RNA_POL_N_sf
IPR029262 RPOL_N
PANTHERiPTHR10102 PTHR10102, 1 hit
PfamiView protein in Pfam
PF00940 RNA_pol, 1 hit
PF14700 RPOL_N, 1 hit
SMARTiView protein in SMART
SM01311 RPOL_N, 1 hit
PROSITEiView protein in PROSITE
PS00900 RNA_POL_PHAGE_1, 1 hit
PS00489 RNA_POL_PHAGE_2, 1 hit

Sequencei

Sequence statusi: Complete.

P00573-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNTINIAKND FSDIELAAIP FNTLADHYGE RLAREQLALE HESYEMGEAR 
        60         70         80         90        100
FRKMFERQLK AGEVADNAAA KPLITTLLPK MIARINDWFE EVKAKRGKRP 
       110        120        130        140        150
TAFQFLQEIK PEAVAYITIK TTLACLTSAD NTTVQAVASA IGRAIEDEAR 
       160        170        180        190        200
FGRIRDLEAK HFKKNVEEQL NKRVGHVYKK AFMQVVEADM LSKGLLGGEA 
       210        220        230        240        250
WSSWHKEDSI HVGVRCIEML IESTGMVSLH RQNAGVVGQD SETIELAPEY 
       260        270        280        290        300
AEAIATRAGA LAGISPMFQP CVVPPKPWTG ITGGGYWANG RRPLALVRTH 
       310        320        330        340        350
SKKALMRYED VYMPEVYKAI NIAQNTAWKI NKKVLAVANV ITKWKHCPVE 
       360        370        380        390        400
DIPAIEREEL PMKPEDIDMN PEALTAWKRA AAAVYRKDKA RKSRRISLEF 
       410        420        430        440        450
MLEQANKFAN HKAIWFPYNM DWRGRVYAVS MFNPQGNDMT KGLLTLAKGK 
       460        470        480        490        500
PIGKEGYYWL KIHGANCAGV DKVPFPERIK FIEENHENIM ACAKSPLENT 
       510        520        530        540        550
WWAEQDSPFC FLAFCFEYAG VQHHGLSYNC SLPLAFDGSC SGIQHFSAML 
       560        570        580        590        600
RDEVGGRAVN LLPSETVQDI YGIVAKKVNE ILQADAINGT DNEVVTVTDE 
       610        620        630        640        650
NTGEISEKVK LGTKALAGQW LAYGVTRSVT KRSVMTLAYG SKEFGFRQQV 
       660        670        680        690        700
LEDTIQPAID SGKGLMFTQP NQAAGYMAKL IWESVSVTVV AAVEAMNWLK 
       710        720        730        740        750
SAAKLLAAEV KDKKTGEILR KRCAVHWVTP DGFPVWQEYK KPIQTRLNLM 
       760        770        780        790        800
FLGQFRLQPT INTNKDSEID AHKQESGIAP NFVHSQDGSH LRKTVVWAHE 
       810        820        830        840        850
KYGIESFALI HDSFGTIPAD AANLFKAVRE TMVDTYESCD VLADFYDQFA 
       860        870        880 
DQLHESQLDK MPALPAKGNL NLRDILESDF AFA
Length:883
Mass (Da):98,855
Last modified:January 11, 2001 - v2
Checksum:i7FBDEAC457842549
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti388 – 424DKARK…MDWRG → TRLASLAVSALSSCLSKPIS LLTIRPSGSLTTWTGAV in CAA24333 (PubMed:7310873).CuratedAdd BLAST37
Sequence conflicti389K → R in AAA32569 (PubMed:6093820).Curated1
Sequence conflicti443L → R in CAA24333 (PubMed:7310873).Curated1
Sequence conflicti474P → S in CAA24333 (PubMed:7310873).Curated1
Sequence conflicti623Y → H in AAA32569 (PubMed:6093820).Curated1
Sequence conflicti665L → P in AAA32569 (PubMed:6093820).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01146 Genomic DNA Translation: CAA24390.1
V01127 Genomic DNA Translation: CAA24333.1
M38308 Genomic DNA Translation: AAA32569.1
PIRiA94615 RNBP17
RefSeqiNP_041960.1, NC_001604.1

Genome annotation databases

GeneIDi1261050
KEGGivg:1261050

Similar proteinsi

Entry informationi

Entry nameiRPOL_BPT7
AccessioniPrimary (citable) accession number: P00573
Secondary accession number(s): Q38543
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 11, 2001
Last modified: February 28, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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