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Protein

Adenylate kinase isoenzyme 1

Gene

AK1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the reversible transfer of the terminal phosphate group between ATP and AMP. Also displays broad nucleoside diphosphate kinase activity. Plays an important role in cellular energy homeostasis and in adenine nucleotide metabolism.UniRule annotation

Catalytic activityi

ATP + AMP = 2 ADP.UniRule annotation
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei39AMPUniRule annotation1
Binding sitei44AMPUniRule annotation1
Binding sitei101AMPUniRule annotation1
Binding sitei132ATPUniRule annotation1
Binding sitei138AMPUniRule annotation1
Binding sitei149AMPUniRule annotation1
Binding sitei177ATP; via carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi18 – 23ATPUniRule annotation6
Nucleotide bindingi65 – 67AMPUniRule annotation3
Nucleotide bindingi94 – 97AMPUniRule annotation4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.4.3 908
ReactomeiR-BTA-499943 Interconversion of nucleotide di- and triphosphates

Names & Taxonomyi

Protein namesi
Recommended name:
Adenylate kinase isoenzyme 1UniRule annotation (EC:2.7.4.3UniRule annotation, EC:2.7.4.6UniRule annotation)
Short name:
AK 1UniRule annotation
Alternative name(s):
ATP-AMP transphosphorylase 1UniRule annotation
ATP:AMP phosphotransferaseUniRule annotation
Adenylate monophosphate kinaseUniRule annotation
MyokinaseUniRule annotation
Gene namesi
Name:AK1UniRule annotation
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 11

Organism-specific databases

VGNCiVGNC:25770 AK1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001589091 – 194Adenylate kinase isoenzyme 1Add BLAST194

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei38PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00570
PeptideAtlasiP00570
PRIDEiP00570

Expressioni

Gene expression databases

BgeeiENSBTAG00000006305 Expressed in 9 organ(s), highest expression level in skeletal muscle tissue

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000050234

Structurei

3D structure databases

ProteinModelPortaliP00570
SMRiP00570
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni38 – 67NMPbindUniRule annotationAdd BLAST30
Regioni131 – 141LIDUniRule annotationAdd BLAST11

Domaini

Consists of three domains, a large central CORE domain and two small peripheral domains, NMPbind and LID, which undergo movements during catalysis. The LID domain closes over the site of phosphoryl transfer upon ATP binding. Assembling and dissambling the active center during each catalytic cycle provides an effective means to prevent ATP hydrolysis.UniRule annotation

Sequence similaritiesi

Belongs to the adenylate kinase family. AK1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiKOG3079 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00390000016215
HOGENOMiHOG000238771
HOVERGENiHBG108060
InParanoidiP00570
KOiK00939
OMAiYPREKNQ
OrthoDBiEOG091G0OQ7
TreeFamiTF354283

Family and domain databases

CDDicd01428 ADK, 1 hit
HAMAPiMF_00235 Adenylate_kinase_Adk, 1 hit
MF_03171 Adenylate_kinase_AK1, 1 hit
InterProiView protein in InterPro
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR028582 AK1
IPR006267 AK1/5
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01360 aden_kin_iso1, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 1 hit

Sequencei

Sequence statusi: Complete.

P00570-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEEKLKKTKI IFVVGGPGSG KGTQCEKIVQ KYGYTHLSTG DLLRAEVSSG
60 70 80 90 100
SARGKMLSEI MEKGQLVPLE TVLDMLRDAM VAKVDTSKGF LIDGYPREVQ
110 120 130 140 150
QGEEFERRIA QPTLLLYVDA GPETMTKRLL KRGETSGRVD DNEETIKKRL
160 170 180 190
ETYYKATEPV IAFYEKRGIV RKVNAEGSVD NVFSQVCTHL DALK
Length:194
Mass (Da):21,664
Last modified:June 27, 2006 - v2
Checksum:i8F6EA93FBE3D3798
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti8T → A AA sequence (PubMed:6089869).Curated1
Sequence conflicti85D → N AA sequence (PubMed:6089869).Curated1
Sequence conflicti98E → Q AA sequence (PubMed:6089869).Curated1
Sequence conflicti126T → Q AA sequence (PubMed:6089869).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020951 mRNA Translation: AAX08968.1
BC114796 mRNA Translation: AAI14797.1
PIRiA00681 KIBOA
RefSeqiNP_001013600.1, NM_001013582.1
XP_005213229.1, XM_005213172.1
UniGeneiBt.4224

Genome annotation databases

EnsembliENSBTAT00000054038; ENSBTAP00000050234; ENSBTAG00000006305
GeneIDi280715
KEGGibta:280715

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT020951 mRNA Translation: AAX08968.1
BC114796 mRNA Translation: AAI14797.1
PIRiA00681 KIBOA
RefSeqiNP_001013600.1, NM_001013582.1
XP_005213229.1, XM_005213172.1
UniGeneiBt.4224

3D structure databases

ProteinModelPortaliP00570
SMRiP00570
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000050234

Proteomic databases

PaxDbiP00570
PeptideAtlasiP00570
PRIDEiP00570

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000054038; ENSBTAP00000050234; ENSBTAG00000006305
GeneIDi280715
KEGGibta:280715

Organism-specific databases

CTDi203
VGNCiVGNC:25770 AK1

Phylogenomic databases

eggNOGiKOG3079 Eukaryota
COG0563 LUCA
GeneTreeiENSGT00390000016215
HOGENOMiHOG000238771
HOVERGENiHBG108060
InParanoidiP00570
KOiK00939
OMAiYPREKNQ
OrthoDBiEOG091G0OQ7
TreeFamiTF354283

Enzyme and pathway databases

BRENDAi2.7.4.3 908
ReactomeiR-BTA-499943 Interconversion of nucleotide di- and triphosphates

Gene expression databases

BgeeiENSBTAG00000006305 Expressed in 9 organ(s), highest expression level in skeletal muscle tissue

Family and domain databases

CDDicd01428 ADK, 1 hit
HAMAPiMF_00235 Adenylate_kinase_Adk, 1 hit
MF_03171 Adenylate_kinase_AK1, 1 hit
InterProiView protein in InterPro
IPR000850 Adenylat/UMP-CMP_kin
IPR033690 Adenylat_kinase_CS
IPR028582 AK1
IPR006267 AK1/5
IPR027417 P-loop_NTPase
PANTHERiPTHR23359 PTHR23359, 1 hit
PRINTSiPR00094 ADENYLTKNASE
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR01360 aden_kin_iso1, 1 hit
PROSITEiView protein in PROSITE
PS00113 ADENYLATE_KINASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKAD1_BOVIN
AccessioniPrimary (citable) accession number: P00570
Secondary accession number(s): Q5E9G9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: June 27, 2006
Last modified: October 10, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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