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UniProtKB - P00552 (KKA2_KLEPN)
Protein
Aminoglycoside 3'-phosphotransferase
Gene
neo
Organism
Klebsiella pneumoniae
Status
Functioni
Resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin and gentamicin B.
Miscellaneous
This enzyme is encoded by the kanamycin and neomycin resistance transposon Tn5. Tn5 was originally isolated from K.pneumoniae, but has been transferred to a number of bacteria including E.coli.
Catalytic activityi
- EC:2.7.1.95
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 190 | Proton acceptor1 Publication | 1 | |
Metal bindingi | 195 | Magnesium | 1 | |
Metal bindingi | 208 | Magnesium | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kanamycin kinase activity Source: UniProtKB-EC
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Antibiotic resistance |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)Alternative name(s): APH(3')-II Short name: APH(3')II Kanamycin kinase, type II Neomycin-kanamycin phosphotransferase type II |
Gene namesi | Name:neo Synonyms:kan, nptII |
Organismi | Klebsiella pneumoniae |
Taxonomic identifieri | 573 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Klebsiella/Raoultella group › Klebsiella |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204804 | 1 – 264 | Aminoglycoside 3'-phosphotransferaseAdd BLAST | 264 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
AlphaFoldDBi | P00552 |
BMRBi | P00552 |
SMRi | P00552 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00552 |
Family & Domainsi
Sequence similaritiesi
Belongs to the aminoglycoside phosphotransferase family.Curated
Family and domain databases
CDDi | cd05150, APH, 1 hit |
InterProi | View protein in InterPro IPR002575, Aminoglycoside_PTrfase IPR024165, Kan/Strep_kinase IPR011009, Kinase-like_dom_sf |
Pfami | View protein in Pfam PF01636, APH, 1 hit |
PIRSFi | PIRSF000706, Kanamycin_kin, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
i Sequence
Sequence statusi: Complete.
P00552-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIEQDGLHAG SPAAWVERLF GYDWAQQTIG CSDAAVFRLS AQGRPVLFVK
60 70 80 90 100
TDLSGALNEL QDEAARLSWL ATTGVPCAAV LDVVTEAGRD WLLLGEVPGQ
110 120 130 140 150
DLLSSHLAPA EKVSIMADAM RRLHTLDPAT CPFDHQAKHR IERARTRMEA
160 170 180 190 200
GLVDQDDLDE EHQGLAPAEL FARLKARMPD GEDLVVTHGD ACLPNIMVEN
210 220 230 240 250
GRFSGFIDCG RLGVADRYQD IALATRDIAE ELGGEWADRF LVLYGIAAPD
260
SQRIAFYRLL DEFF
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00004 Unassigned DNA Translation: AAA73390.1 V00618 Genomic DNA Translation: CAA23892.1 U32991 Genomic DNA Translation: AAA85506.1 X01702 Genomic DNA Translation: CAA25852.1 U66885 Genomic DNA Translation: AAC48873.1 |
PIRi | A00663, PKECT5 |
RefSeqi | WP_000572405.1, NZ_VKSL01000036.1 YP_008531444.1, NC_022333.1 YP_788126.1, NC_008460.1 |
Genome annotation databases
GeneIDi | 61686716 |
KEGGi | ag:CAA23892 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U00004 Unassigned DNA Translation: AAA73390.1 V00618 Genomic DNA Translation: CAA23892.1 U32991 Genomic DNA Translation: AAA85506.1 X01702 Genomic DNA Translation: CAA25852.1 U66885 Genomic DNA Translation: AAC48873.1 |
PIRi | A00663, PKECT5 |
RefSeqi | WP_000572405.1, NZ_VKSL01000036.1 YP_008531444.1, NC_022333.1 YP_788126.1, NC_008460.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1ND4 | X-ray | 2.10 | A/B | 1-264 | [»] | |
AlphaFoldDBi | P00552 | |||||
BMRBi | P00552 | |||||
SMRi | P00552 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Genome annotation databases
GeneIDi | 61686716 |
KEGGi | ag:CAA23892 |
Miscellaneous databases
EvolutionaryTracei | P00552 |
Family and domain databases
CDDi | cd05150, APH, 1 hit |
InterProi | View protein in InterPro IPR002575, Aminoglycoside_PTrfase IPR024165, Kan/Strep_kinase IPR011009, Kinase-like_dom_sf |
Pfami | View protein in Pfam PF01636, APH, 1 hit |
PIRSFi | PIRSF000706, Kanamycin_kin, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KKA2_KLEPN | |
Accessioni | P00552Primary (citable) accession number: P00552 Secondary accession number(s): Q6LCR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | May 25, 2022 | |
This is version 106 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Transposable elementDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families