Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Aminoglycoside 3'-phosphotransferase

Gene

neo

Organism
Klebsiella pneumoniae
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Resistance to kanamycin, neomycin, paromomycin, ribostamycin, butirosin and gentamicin B.

Miscellaneous

This enzyme is encoded by the kanamycin and neomycin resistance transposon Tn5. Tn5 was originally isolated from K.pneumoniae, but has been transferred to a number of bacteria including E.coli.

Catalytic activityi

ATP + kanamycin = ADP + kanamycin 3'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei190Proton acceptor1 Publication1
Metal bindingi195Magnesium1
Metal bindingi208Magnesium1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processAntibiotic resistance
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)
Alternative name(s):
APH(3')-II
Short name:
APH(3')II
Kanamycin kinase, type II
Neomycin-kanamycin phosphotransferase type II
Gene namesi
Name:neo
Synonyms:kan, nptII
OrganismiKlebsiella pneumoniae
Taxonomic identifieri573 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeKlebsiella

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002048041 – 264Aminoglycoside 3'-phosphotransferaseAdd BLAST264

Structurei

Secondary structure

1264
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00552
SMRiP00552
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00552

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

KOiK19300

Family and domain databases

CDDicd05150 APH, 1 hit
InterProiView protein in InterPro
IPR002575 Aminoglycoside_PTrfase
IPR024165 Kan/Strep_kinase
IPR011009 Kinase-like_dom_sf
PfamiView protein in Pfam
PF01636 APH, 1 hit
PIRSFiPIRSF000706 Kanamycin_kin, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit

Sequencei

Sequence statusi: Complete.

P00552-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIEQDGLHAG SPAAWVERLF GYDWAQQTIG CSDAAVFRLS AQGRPVLFVK
60 70 80 90 100
TDLSGALNEL QDEAARLSWL ATTGVPCAAV LDVVTEAGRD WLLLGEVPGQ
110 120 130 140 150
DLLSSHLAPA EKVSIMADAM RRLHTLDPAT CPFDHQAKHR IERARTRMEA
160 170 180 190 200
GLVDQDDLDE EHQGLAPAEL FARLKARMPD GEDLVVTHGD ACLPNIMVEN
210 220 230 240 250
GRFSGFIDCG RLGVADRYQD IALATRDIAE ELGGEWADRF LVLYGIAAPD
260
SQRIAFYRLL DEFF
Length:264
Mass (Da):29,048
Last modified:July 21, 1986 - v1
Checksum:iCB0507C1C30863BC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00004 Unassigned DNA Translation: AAA73390.1
V00618 Genomic DNA Translation: CAA23892.1
U32991 Genomic DNA Translation: AAA85506.1
X01702 Genomic DNA Translation: CAA25852.1
U66885 Genomic DNA Translation: AAC48873.1
PIRiA00663 PKECT5
RefSeqiWP_000572405.1, NZ_PWBH01000180.1

Genome annotation databases

KEGGiag:CAA23892

Similar proteinsi

Entry informationi

Entry nameiKKA2_KLEPN
AccessioniPrimary (citable) accession number: P00552
Secondary accession number(s): Q6LCR6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: July 18, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Transposable element
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again