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UniProtKB - P00551 (KKA1_ECOLX)
Protein
Aminoglycoside 3'-phosphotransferase
Gene
aphA1
Organism
Escherichia coli
Status
Functioni
Resistance to kanamycin and structurally-related aminoglycosides, including amikacin.
Miscellaneous
This enzyme is encoded by the kanamycin resistance transposon Tn903.
Caution
PubMed:2823223 strain is not known and has been termed 'X' in this entry.Curated
Was originally (Ref. 4) termed APH(3')IIa whereas it is APH(3')Ia.Curated
Catalytic activityi
- EC:2.7.1.95
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 198 | Proton acceptorBy similarity | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kanamycin kinase activity Source: UniProtKB-EC
GO - Biological processi
- response to antibiotic Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Antibiotic resistance |
Ligand | ATP-binding, Nucleotide-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Aminoglycoside 3'-phosphotransferase (EC:2.7.1.95)Alternative name(s): APH(3')-I Short name: APH(3')I Kanamycin kinase, type I Neomycin-kanamycin phosphotransferase type I |
Gene namesi | Name:aphA1 Synonyms:nptI |
Encoded oni | Plasmid Rts11 Publication Plasmid pIP15181 Publication |
Organismi | Escherichia coli |
Taxonomic identifieri | 562 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Enterobacteriaceae › Escherichia |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000204802 | 1 – 271 | Aminoglycoside 3'-phosphotransferaseAdd BLAST | 271 |
Family & Domainsi
Sequence similaritiesi
Belongs to the aminoglycoside phosphotransferase family.Curated
Family and domain databases
CDDi | cd05150, APH, 1 hit |
InterProi | View protein in InterPro IPR002575, Aminoglycoside_PTrfase IPR024165, Kan/Strep_kinase IPR011009, Kinase-like_dom_sf |
Pfami | View protein in Pfam PF01636, APH, 1 hit |
PIRSFi | PIRSF000706, Kanamycin_kin, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
i Sequence
Sequence statusi: Complete.
P00551-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSHIQRETSC SRPRLNSNLD ADLYGYKWAR DNVGQSGATI YRLYGKPDAP
60 70 80 90 100
ELFLKHGKGS VANDVTDEMV RLNWLTEFMP LPTIKHFIRT PDDAWLLTTA
110 120 130 140 150
IPGKTAFQVL EEYPDSGENI VDALAVFLRR LHSIPVCNCP FNSDRVFRLA
160 170 180 190 200
QAQSRMNNGL VDASDFDDER NGWPVEQVWK EMHKLLPFSP DSVVTHGDFS
210 220 230 240 250
LDNLIFDEGK LIGCIDVGRV GIADRYQDLA ILWNCLGEFS PSLQKRLFQK
260 270
YGIDNPDMNK LQFHLMLDEF F
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 19 | L → M (PubMed:6270337).Curated | 1 | |
Sequence conflicti | 19 | L → M (Ref. 4) Curated | 1 | |
Sequence conflicti | 48 | D → N in CAA68507 (PubMed:2823223).Curated | 1 | |
Sequence conflicti | 120 | I → N in CAA68507 (PubMed:2823223).Curated | 1 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 27 | K → R in plasmid Rts1 and strain X. | 1 | |
Natural varianti | 77 | E → A in plasmid Rts1 and strain X. | 1 | |
Natural varianti | 126 | V → A in plasmid Rts1. | 1 | |
Natural varianti | 150 | A → T in plasmid Rts1. | 1 | |
Natural varianti | 247 | L → F in plasmid Rts1. | 1 | |
Natural varianti | 249 | Q → Y in plasmid Rts1. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00359 Genomic DNA Translation: CAA23656.1 Y00452 Genomic DNA Translation: CAA68507.1 M65202 Unassigned DNA Translation: AAA72095.1 AF498082 Genomic DNA Translation: AAN59785.1 S54065 Genomic DNA Translation: AAD13871.1 V00621 Genomic DNA Translation: CAA23898.1 M10596 Genomic DNA Translation: AAA27445.1 M10597 Genomic DNA Translation: AAA27446.1 V00293 Genomic DNA Translation: CAA23568.1 |
PIRi | A00662, PKECT9 |
RefSeqi | WP_000018322.1, NZ_WVVH01000102.1 WP_063841683.1, NG_047429.1 WP_063842148.1, NG_047437.1 YP_001687822.1, NC_004429.1 YP_002891173.1, NC_012690.1 YP_002995708.1, NC_012886.1 YP_008864028.1, NC_022992.1 YP_008864695.1, NC_022996.1 YP_008995202.1, NC_023277.2 YP_008997405.1, NC_023289.2 |
Genome annotation databases
KEGGi | ag:CAA23656 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00359 Genomic DNA Translation: CAA23656.1 Y00452 Genomic DNA Translation: CAA68507.1 M65202 Unassigned DNA Translation: AAA72095.1 AF498082 Genomic DNA Translation: AAN59785.1 S54065 Genomic DNA Translation: AAD13871.1 V00621 Genomic DNA Translation: CAA23898.1 M10596 Genomic DNA Translation: AAA27445.1 M10597 Genomic DNA Translation: AAA27446.1 V00293 Genomic DNA Translation: CAA23568.1 |
PIRi | A00662, PKECT9 |
RefSeqi | WP_000018322.1, NZ_WVVH01000102.1 WP_063841683.1, NG_047429.1 WP_063842148.1, NG_047437.1 YP_001687822.1, NC_004429.1 YP_002891173.1, NC_012690.1 YP_002995708.1, NC_012886.1 YP_008864028.1, NC_022992.1 YP_008864695.1, NC_022996.1 YP_008995202.1, NC_023277.2 YP_008997405.1, NC_023289.2 |
3D structure databases
AlphaFoldDBi | P00551 |
SMRi | P00551 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | P00551, 2 interactors |
MINTi | P00551 |
Chemistry databases
ChEMBLi | CHEMBL3751655 |
DrugBanki | DB01172, Kanamycin |
Genome annotation databases
KEGGi | ag:CAA23656 |
Family and domain databases
CDDi | cd05150, APH, 1 hit |
InterProi | View protein in InterPro IPR002575, Aminoglycoside_PTrfase IPR024165, Kan/Strep_kinase IPR011009, Kinase-like_dom_sf |
Pfami | View protein in Pfam PF01636, APH, 1 hit |
PIRSFi | PIRSF000706, Kanamycin_kin, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KKA1_ECOLX | |
Accessioni | P00551Primary (citable) accession number: P00551 Secondary accession number(s): Q53299, Q8GNQ0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 123 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Plasmid, Transposable elementDocuments
- SIMILARITY comments
Index of protein domains and families