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Protein

Homoserine kinase

Gene

thrB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score:

Annotation score:4 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Catalyzes the ATP-dependent phosphorylation of L-homoserine to L-homoserine phosphate. Is also able to phosphorylate the hydroxy group on gamma-carbon of L-homoserine analogs when the functional group at the alpha-position is a carboxyl, an ester, or even a hydroxymethyl group. Neither L-threonine nor L-serine are substrates of the enzyme.1 Publication

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the ‘Function’ section describes biophysical and chemical properties, such as maximal absorption, kinetic parameters, pH dependence, redox potentials and temperature dependence.<p><a href='/help/biophysicochemical_properties' target='_top'>More...</a></p>Kineticsi

The catalytic efficiency is 500-fold higher with L-homoserine than with D-homoserine as substrate.
  1. KM=0.14 mM for L-homoserine1 Publication
  2. KM=31.8 mM for D-homoserine1 Publication
  3. KM=0.13 mM for ATP1 Publication

    <p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-threonine biosynthesis

    This protein is involved in step 4 of the subpathway that synthesizes L-threonine from L-aspartate.
    Proteins known to be involved in the 5 steps of the subpathway in this organism are:
    1. Bifunctional aspartokinase/homoserine dehydrogenase 1 (thrA), Bifunctional aspartokinase/homoserine dehydrogenase 2 (metL)
    2. Aspartate-semialdehyde dehydrogenase (asd)
    3. Bifunctional aspartokinase/homoserine dehydrogenase 1 (thrA), Bifunctional aspartokinase/homoserine dehydrogenase 2 (metL)
    4. Homoserine kinase (thrB)
    5. Threonine synthase (thrC)
    This subpathway is part of the pathway L-threonine biosynthesis, which is itself part of Amino-acid biosynthesis.
    View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-threonine from L-aspartate, the pathway L-threonine biosynthesis and in Amino-acid biosynthesis.

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘Function’ section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi91 – 101ATPSequence analysisAdd BLAST11

    <p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

    • ATP binding Source: UniProtKB-UniRule
    • homoserine kinase activity Source: EcoCyc

    GO - Biological processi

    • threonine biosynthetic process Source: EcoCyc

    <p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

    Molecular functionKinase, Transferase
    Biological processAmino-acid biosynthesis, Threonine biosynthesis
    LigandATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyc Collection of Pathway/Genome Databases

    More...
    BioCyci
    EcoCyc:HOMOSERKIN-MONOMER
    MetaCyc:HOMOSERKIN-MONOMER

    UniPathway: a resource for the exploration and annotation of metabolic pathways

    More...
    UniPathwayi
    UPA00050;UER00064

    <p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
    Recommended name:
    Homoserine kinase (EC:2.7.1.39)
    Short name:
    HK
    Short name:
    HSK
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
    Name:thrB
    Ordered Locus Names:b0003, JW0002
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiEscherichia coli (strain K12)
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri83333 [NCBI]
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
    <p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
    • UP000000318 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome
    • UP000000625 Componenti: Chromosome

    Organism-specific databases

    Escherichia coli strain K12 genome database

    More...
    EcoGenei
    EG10999 thrB

    <p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Cytoplasm

    <p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the <a href="http://www.uniprot.org/manual/pathology_and_biotech_section">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi139H → L: 35-fold decrease in kinase activity. 1 Publication1
    Mutagenesisi203H → L: 2-fold decrease in kinase activity but nearly no change in substrates affinity. 1 Publication1
    Mutagenesisi206H → Q: 3500-fold decrease in kinase activity. 1 Publication1
    Mutagenesisi235R → H: 250-fold decrease in kinase activity but no change in substrates affinity. 1 Publication1
    Mutagenesisi235R → L: 26200-fold decrease in catalytic efficiency. 1 Publication1

    <p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    <p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001565671 – 310Homoserine kinaseAdd BLAST310

    Proteomic databases

    PaxDb, a database of protein abundance averages across all three domains of life

    More...
    PaxDbi
    P00547

    PRoteomics IDEntifications database

    More...
    PRIDEi
    P00547

    2D gel databases

    Two-dimensional polyacrylamide gel electrophoresis database from the Geneva University Hospital

    More...
    SWISS-2DPAGEi
    P00547

    <p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

    Protein-protein interaction databases

    The Biological General Repository for Interaction Datasets (BioGrid)

    More...
    BioGridi
    4261934, 9 interactors

    Protein interaction database and analysis system

    More...
    IntActi
    P00547, 2 interactors

    STRING: functional protein association networks

    More...
    STRINGi
    316385.ECDH10B_0003

    Chemistry databases

    BindingDB database of measured binding affinities

    More...
    BindingDBi
    P00547

    <p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

    3D structure databases

    Protein Model Portal of the PSI-Nature Structural Biology Knowledgebase

    More...
    ProteinModelPortali
    P00547

    SWISS-MODEL Repository - a database of annotated 3D protein structure models

    More...
    SMRi
    P00547

    Database of comparative protein structure models

    More...
    ModBasei
    Search...

    MobiDB: a database of protein disorder and mobility annotations

    More...
    MobiDBi
    Search...

    <p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

    <p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

    Phylogenomic databases

    evolutionary genealogy of genes: Non-supervised Orthologous Groups

    More...
    eggNOGi
    ENOG4105D5I Bacteria
    COG0083 LUCA

    The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

    More...
    HOGENOMi
    HOG000247198

    InParanoid: Eukaryotic Ortholog Groups

    More...
    InParanoidi
    P00547

    KEGG Orthology (KO)

    More...
    KOi
    K00872

    Database for complete collections of gene phylogenies

    More...
    PhylomeDBi
    P00547

    Family and domain databases

    Gene3D Structural and Functional Annotation of Protein Families

    More...
    Gene3Di
    3.30.230.10, 1 hit
    3.30.70.890, 1 hit

    HAMAP database of protein families

    More...
    HAMAPi
    MF_00384 Homoser_kinase, 1 hit

    Integrated resource of protein families, domains and functional sites

    More...
    InterProi
    View protein in InterPro
    IPR013750 GHMP_kinase_C_dom
    IPR036554 GHMP_kinase_C_sf
    IPR006204 GHMP_kinase_N_dom
    IPR006203 GHMP_knse_ATP-bd_CS
    IPR000870 Homoserine_kinase
    IPR020568 Ribosomal_S5_D2-typ_fold
    IPR014721 Ribosomal_S5_D2-typ_fold_subgr

    The PANTHER Classification System

    More...
    PANTHERi
    PTHR20861:SF1 PTHR20861:SF1, 1 hit

    Pfam protein domain database

    More...
    Pfami
    View protein in Pfam
    PF08544 GHMP_kinases_C, 1 hit
    PF00288 GHMP_kinases_N, 1 hit

    PIRSF; a whole-protein classification database

    More...
    PIRSFi
    PIRSF000676 Homoser_kin, 1 hit

    Superfamily database of structural and functional annotation

    More...
    SUPFAMi
    SSF54211 SSF54211, 1 hit
    SSF55060 SSF55060, 1 hit

    TIGRFAMs; a protein family database

    More...
    TIGRFAMsi
    TIGR00191 thrB, 1 hit

    PROSITE; a protein domain and family database

    More...
    PROSITEi
    View protein in PROSITE
    PS00627 GHMP_KINASES_ATP, 1 hit

    <p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

    <p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

    P00547-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MVKVYAPASS ANMSVGFDVL GAAVTPVDGA LLGDVVTVEA AETFSLNNLG
    60 70 80 90 100
    RFADKLPSEP RENIVYQCWE RFCQELGKQI PVAMTLEKNM PIGSGLGSSA
    110 120 130 140 150
    CSVVAALMAM NEHCGKPLND TRLLALMGEL EGRISGSIHY DNVAPCFLGG
    160 170 180 190 200
    MQLMIEENDI ISQQVPGFDE WLWVLAYPGI KVSTAEARAI LPAQYRRQDC
    210 220 230 240 250
    IAHGRHLAGF IHACYSRQPE LAAKLMKDVI AEPYRERLLP GFRQARQAVA
    260 270 280 290 300
    EIGAVASGIS GSGPTLFALC DKPETAQRVA DWLGKNYLQN QEGFVHICRL
    310
    DTAGARVLEN
    Length:310
    Mass (Da):33,624
    Last modified:April 1, 1993 - v2
    <p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i0F225F9F1B634BE8
    GO

    Sequence databases

    Select the link destinations:

    EMBL nucleotide sequence database

    More...
    EMBLi

    GenBank nucleotide sequence database

    More...
    GenBanki

    DNA Data Bank of Japan; a nucleotide sequence database

    More...
    DDBJi
    Links Updated
    L13601 Genomic DNA Translation: AAA20618.1
    U14003 Genomic DNA Translation: AAA97302.1
    U00096 Genomic DNA Translation: AAC73114.1
    AP009048 Genomic DNA Translation: BAB96580.2

    Protein sequence database of the Protein Information Resource

    More...
    PIRi
    S56630 KIECM

    NCBI Reference Sequences

    More...
    RefSeqi
    NP_414544.1, NC_000913.3
    WP_000241662.1, NZ_LN832404.1

    Genome annotation databases

    Ensembl bacterial and archaeal genome annotation project

    More...
    EnsemblBacteriai
    AAC73114; AAC73114; b0003
    BAB96580; BAB96580; BAB96580

    Database of genes from NCBI RefSeq genomes

    More...
    GeneIDi
    947498

    KEGG: Kyoto Encyclopedia of Genes and Genomes

    More...
    KEGGi
    ecj:JW0002
    eco:b0003

    Pathosystems Resource Integration Center (PATRIC)

    More...
    PATRICi
    fig|1411691.4.peg.2280

    <p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

    <p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L13601 Genomic DNA Translation: AAA20618.1
    U14003 Genomic DNA Translation: AAA97302.1
    U00096 Genomic DNA Translation: AAC73114.1
    AP009048 Genomic DNA Translation: BAB96580.2
    PIRiS56630 KIECM
    RefSeqiNP_414544.1, NC_000913.3
    WP_000241662.1, NZ_LN832404.1

    3D structure databases

    ProteinModelPortaliP00547
    SMRiP00547
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi4261934, 9 interactors
    IntActiP00547, 2 interactors
    STRINGi316385.ECDH10B_0003

    Chemistry databases

    BindingDBiP00547

    2D gel databases

    SWISS-2DPAGEiP00547

    Proteomic databases

    PaxDbiP00547
    PRIDEiP00547

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsemblBacteriaiAAC73114; AAC73114; b0003
    BAB96580; BAB96580; BAB96580
    GeneIDi947498
    KEGGiecj:JW0002
    eco:b0003
    PATRICifig|1411691.4.peg.2280

    Organism-specific databases

    EchoBASE - an integrated post-genomic database for E. coli

    More...
    EchoBASEi
    EB0992
    EcoGeneiEG10999 thrB

    Phylogenomic databases

    eggNOGiENOG4105D5I Bacteria
    COG0083 LUCA
    HOGENOMiHOG000247198
    InParanoidiP00547
    KOiK00872
    PhylomeDBiP00547

    Enzyme and pathway databases

    UniPathwayi
    UPA00050;UER00064

    BioCyciEcoCyc:HOMOSERKIN-MONOMER
    MetaCyc:HOMOSERKIN-MONOMER

    Miscellaneous databases

    Protein Ontology

    More...
    PROi
    PR:P00547

    Family and domain databases

    Gene3Di3.30.230.10, 1 hit
    3.30.70.890, 1 hit
    HAMAPiMF_00384 Homoser_kinase, 1 hit
    InterProiView protein in InterPro
    IPR013750 GHMP_kinase_C_dom
    IPR036554 GHMP_kinase_C_sf
    IPR006204 GHMP_kinase_N_dom
    IPR006203 GHMP_knse_ATP-bd_CS
    IPR000870 Homoserine_kinase
    IPR020568 Ribosomal_S5_D2-typ_fold
    IPR014721 Ribosomal_S5_D2-typ_fold_subgr
    PANTHERiPTHR20861:SF1 PTHR20861:SF1, 1 hit
    PfamiView protein in Pfam
    PF08544 GHMP_kinases_C, 1 hit
    PF00288 GHMP_kinases_N, 1 hit
    PIRSFiPIRSF000676 Homoser_kin, 1 hit
    SUPFAMiSSF54211 SSF54211, 1 hit
    SSF55060 SSF55060, 1 hit
    TIGRFAMsiTIGR00191 thrB, 1 hit
    PROSITEiView protein in PROSITE
    PS00627 GHMP_KINASES_ATP, 1 hit

    ProtoNet; Automatic hierarchical classification of proteins

    More...
    ProtoNeti
    Search...

    <p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

    <p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiKHSE_ECOLI
    <p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P00547
    <p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
    Last sequence update: April 1, 1993
    Last modified: December 5, 2018
    This is version 155 of the entry and version 2 of the sequence. See complete history.
    <p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    <p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
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