UniProtKB - P00546 (CDK1_YEAST)
Cyclin-dependent kinase 1
CDC28
Functioni
Cyclin-dependent kinase that acts as a master regulator of the mitotic and meiotic cell cycles (By similarity).
Required to drive the G1-S transition (PubMed:2664468).
More than 200 substrates have been identified (PubMed:14574415).
Substrate specificity is in part regulated by the bound cyclin protein (By similarity).
Phosphorylates YTA7 during S-phase to promote transcription of histones (PubMed:22156209).
By similarity3 PublicationsMiscellaneous
Catalytic activityi
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 40 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 136 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 14 – 22 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- cyclin binding Source: GO_Central
- cyclin-dependent protein serine/threonine kinase activity Source: SGD
- histone binding Source: SGD
- protein kinase activity Source: SGD
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: UniProtKB
- protein serine kinase activity Source: RHEA
- RNA polymerase II complex binding Source: SGD
GO - Biological processi
- 7-methylguanosine mRNA capping Source: SGD
- cell division Source: UniProtKB-KW
- cellular protein catabolic process Source: SGD
- cellular response to DNA damage stimulus Source: ComplexPortal
- DNA double-strand break processing Source: SGD
- double-strand break repair via nonhomologous end joining Source: SGD
- G1/S transition of mitotic cell cycle Source: GO_Central
- G2/M transition of mitotic cell cycle Source: ComplexPortal
- meiotic DNA double-strand break processing Source: SGD
- mitotic sister chromatid biorientation Source: SGD
- mitotic spindle assembly Source: SGD
- negative regulation of DNA replication origin binding Source: SGD
- negative regulation of double-strand break repair via nonhomologous end joining Source: SGD
- negative regulation of meiotic cell cycle Source: SGD
- negative regulation of mitotic actomyosin contractile ring assembly Source: SGD
- negative regulation of mitotic cell cycle Source: SGD
- negative regulation of sister chromatid cohesion Source: SGD
- negative regulation of transcription, DNA-templated Source: SGD
- negative regulation of transcription by RNA polymerase II Source: SGD
- peptidyl-serine phosphorylation Source: SGD
- peptidyl-threonine phosphorylation Source: SGD
- phosphorylation of RNA polymerase II C-terminal domain Source: SGD
- positive regulation of double-strand break repair via homologous recombination Source: SGD
- positive regulation of glycogen catabolic process Source: SGD
- positive regulation of meiotic cell cycle Source: SGD
- positive regulation of mitotic cell cycle Source: SGD
- positive regulation of mitotic DNA damage checkpoint Source: SGD
- positive regulation of mitotic spindle pole body separation Source: SGD
- positive regulation of nuclear cell cycle DNA replication Source: SGD
- positive regulation of transcription, DNA-templated Source: SGD
- positive regulation of transcription by RNA polymerase II Source: SGD
- positive regulation of trehalose catabolic process Source: SGD
- positive regulation of triglyceride catabolic process Source: SGD
- post-Golgi vesicle-mediated transport Source: SGD
- protein localization to nuclear periphery Source: SGD
- protein localization to nucleus Source: SGD
- protein localization to spindle microtubule Source: SGD
- protein phosphorylation Source: SGD
- regulation of budding cell apical bud growth Source: SGD
- regulation of chromatin organization Source: UniProtKB
- regulation of filamentous growth Source: SGD
- regulation of G2/M transition of mitotic cell cycle Source: GO_Central
- regulation of gene expression Source: GO_Central
- regulation of nucleosome density Source: SGD
- regulation of protein localization Source: SGD
- regulation of protein localization to chromatin Source: SGD
- regulation of spindle assembly Source: SGD
- regulation of telomere maintenance via telomerase Source: SGD
- regulation of transcription involved in G1/S transition of mitotic cell cycle Source: ComplexPortal
- signal transduction Source: GO_Central
- synaptonemal complex assembly Source: SGD
- telomere maintenance Source: SGD
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Cell cycle, Cell division, Mitosis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 984 |
Reactomei | R-SCE-3214858, RMTs methylate histone arginines R-SCE-6804757, Regulation of TP53 Degradation R-SCE-68949, Orc1 removal from chromatin R-SCE-68962, Activation of the pre-replicative complex R-SCE-69017, CDK-mediated phosphorylation and removal of Cdc6 R-SCE-69202, Cyclin E associated events during G1/S transition R-SCE-69231, Cyclin D associated events in G1 R-SCE-69656, Cyclin A:Cdk2-associated events at S phase entry R-SCE-75815, Ubiquitin-dependent degradation of Cyclin D |
Protein family/group databases
MoonDBi | P00546, Predicted |
Names & Taxonomyi
Protein namesi | Recommended name: Cyclin-dependent kinase 11 Publication (EC:2.7.11.22By similarity)Short name: CDK11 Publication Alternative name(s): Cell division control protein 28 Cell division protein kinase 1 |
Gene namesi | Ordered Locus Names:YBR160WImported ORF Names:YBR1211 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000000364, CDC28 |
VEuPathDBi | FungiDB:YBR160W |
Subcellular locationi
Endoplasmic reticulum
- endoplasmic reticulum Source: SGD
Nucleus
- nucleus Source: SGD
Other locations
- cellular bud neck Source: SGD
- cyclin-dependent protein kinase holoenzyme complex Source: SGD
- cytoplasm Source: SGD
- cytoplasmic stress granule Source: SGD
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedCombined sources | |||
ChainiPRO_0000085722 | 2 – 298 | Cyclin-dependent kinase 1Add BLAST | 297 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineCombined sources | 1 | |
Modified residuei | 19 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 169 | PhosphothreonineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
MaxQBi | P00546 |
PaxDbi | P00546 |
PRIDEi | P00546 |
PTM databases
iPTMneti | P00546 |
Interactioni
Subunit structurei
Forms a stable but non-covalent complex with the CKS1 protein and with a cyclin.
1 PublicationBinary interactionsi
P00546
GO - Molecular functioni
- cyclin binding Source: GO_Central
- histone binding Source: SGD
- RNA polymerase II complex binding Source: SGD
Protein-protein interaction databases
BioGRIDi | 32859, 1556 interactors |
ComplexPortali | CPX-1699, CLN1-CDC28 kinase complex CPX-1700, CLN3-CDC28 kinase complex CPX-1701, CLB2-CDC28 kinase complex CPX-1702, CLB5-CDC28 kinase complex CPX-335, CLB1-CDC28 kinase complex CPX-336, CLB3-CDC28 kinase complex CPX-337, CLB4-CDC28 kinase complex CPX-339, CLB6-CDC28 kinase complex CPX-342, CLN2-CDC28 kinase complex |
DIPi | DIP-1039N |
IntActi | P00546, 87 interactors |
MINTi | P00546 |
STRINGi | 4932.YBR160W |
Chemistry databases
BindingDBi | P00546 |
Miscellaneous databases
RNActi | P00546, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 8 – 295 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 288 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
GeneTreei | ENSGT00940000153335 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P00546 |
OMAi | NYTHEIV |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MSGELANYKR LEKVGEGTYG VVYKALDLRP GQGQRVVALK KIRLESEDEG
60 70 80 90 100
VPSTAIREIS LLKELKDDNI VRLYDIVHSD AHKLYLVFEF LDLDLKRYME
110 120 130 140 150
GIPKDQPLGA DIVKKFMMQL CKGIAYCHSH RILHRDLKPQ NLLINKDGNL
160 170 180 190 200
KLGDFGLARA FGVPLRAYTH EIVTLWYRAP EVLLGGKQYS TGVDTWSIGC
210 220 230 240 250
IFAEMCNRKP IFSGDSEIDQ IFKIFRVLGT PNEAIWPDIV YLPDFKPSFP
260 270 280 290
QWRRKDLSQV VPSLDPRGID LLDKLLAYDP INRISARRAA IHPYFQES
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00257 Genomic DNA Translation: CAA25065.1 Z36029 Genomic DNA Translation: CAA85119.1 X80224 Genomic DNA Translation: CAA56509.1 BK006936 Genomic DNA Translation: DAA07275.1 |
PIRi | A00657, TVBY8 |
RefSeqi | NP_009718.3, NM_001178508.3 |
Genome annotation databases
EnsemblFungii | YBR160W_mRNA; YBR160W; YBR160W |
GeneIDi | 852457 |
KEGGi | sce:YBR160W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00257 Genomic DNA Translation: CAA25065.1 Z36029 Genomic DNA Translation: CAA85119.1 X80224 Genomic DNA Translation: CAA56509.1 BK006936 Genomic DNA Translation: DAA07275.1 |
PIRi | A00657, TVBY8 |
RefSeqi | NP_009718.3, NM_001178508.3 |
3D structure databases
SMRi | P00546 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 32859, 1556 interactors |
ComplexPortali | CPX-1699, CLN1-CDC28 kinase complex CPX-1700, CLN3-CDC28 kinase complex CPX-1701, CLB2-CDC28 kinase complex CPX-1702, CLB5-CDC28 kinase complex CPX-335, CLB1-CDC28 kinase complex CPX-336, CLB3-CDC28 kinase complex CPX-337, CLB4-CDC28 kinase complex CPX-339, CLB6-CDC28 kinase complex CPX-342, CLN2-CDC28 kinase complex |
DIPi | DIP-1039N |
IntActi | P00546, 87 interactors |
MINTi | P00546 |
STRINGi | 4932.YBR160W |
Chemistry databases
BindingDBi | P00546 |
ChEMBLi | CHEMBL5213 |
Protein family/group databases
MoonDBi | P00546, Predicted |
PTM databases
iPTMneti | P00546 |
Proteomic databases
MaxQBi | P00546 |
PaxDbi | P00546 |
PRIDEi | P00546 |
Genome annotation databases
EnsemblFungii | YBR160W_mRNA; YBR160W; YBR160W |
GeneIDi | 852457 |
KEGGi | sce:YBR160W |
Organism-specific databases
SGDi | S000000364, CDC28 |
VEuPathDBi | FungiDB:YBR160W |
Phylogenomic databases
eggNOGi | KOG0594, Eukaryota |
GeneTreei | ENSGT00940000153335 |
HOGENOMi | CLU_000288_181_1_1 |
InParanoidi | P00546 |
OMAi | NYTHEIV |
Enzyme and pathway databases
BRENDAi | 2.7.11.22, 984 |
Reactomei | R-SCE-3214858, RMTs methylate histone arginines R-SCE-6804757, Regulation of TP53 Degradation R-SCE-68949, Orc1 removal from chromatin R-SCE-68962, Activation of the pre-replicative complex R-SCE-69017, CDK-mediated phosphorylation and removal of Cdc6 R-SCE-69202, Cyclin E associated events during G1/S transition R-SCE-69231, Cyclin D associated events in G1 R-SCE-69656, Cyclin A:Cdk2-associated events at S phase entry R-SCE-75815, Ubiquitin-dependent degradation of Cyclin D |
Miscellaneous databases
PROi | PR:P00546 |
RNActi | P00546, protein |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CDK1_YEAST | |
Accessioni | P00546Primary (citable) accession number: P00546 Secondary accession number(s): D6VQF5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | February 23, 2022 | |
This is version 228 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome II
Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names - SIMILARITY comments
Index of protein domains and families