UniProtKB - P00528 (SRC64_DROME)
Protein
Tyrosine-protein kinase Src64B
Gene
Src64B
Organism
Drosophila melanogaster (Fruit fly)
Status
Functioni
May play a role in the development of neural tissue and smooth muscle.1 Publication
Catalytic activityi
- EC:2.7.10.2PROSITE-ProRule annotation
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 312 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 404 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 290 – 298 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- non-membrane spanning protein tyrosine kinase activity Source: FlyBase
- signaling receptor binding Source: FlyBase
GO - Biological processi
- actin cytoskeleton organization Source: FlyBase
- activation of MAPKKK activity Source: FlyBase
- adherens junction organization Source: FlyBase
- axon guidance Source: FlyBase
- cell differentiation Source: GO_Central
- cellularization Source: FlyBase
- epithelial cell-cell adhesion Source: FlyBase
- female germline ring canal formation Source: FlyBase
- female germline ring canal formation, actin assembly Source: FlyBase
- female germline ring canal stabilization Source: FlyBase
- filtration diaphragm assembly Source: FlyBase
- germarium-derived egg chamber formation Source: FlyBase
- germarium-derived female germ-line cyst encapsulation Source: FlyBase
- long-term memory Source: FlyBase
- mushroom body development Source: FlyBase
- negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
- oocyte karyosome formation Source: FlyBase
- oogenesis Source: FlyBase
- open tracheal system development Source: FlyBase
- ovarian fusome organization Source: FlyBase
- ovarian nurse cell to oocyte transport Source: FlyBase
- parallel actin filament bundle assembly Source: FlyBase
- peptidyl-tyrosine autophosphorylation Source: GO_Central
- peptidyl-tyrosine phosphorylation Source: FlyBase
- positive regulation of epidermal growth factor receptor signaling pathway Source: FlyBase
- positive regulation of fibroblast growth factor receptor signaling pathway Source: FlyBase
- positive regulation of sevenless signaling pathway Source: FlyBase
- positive regulation of torso signaling pathway Source: FlyBase
- regulation of actin polymerization or depolymerization Source: FlyBase
- regulation of cell population proliferation Source: GO_Central
- regulation of synaptic plasticity Source: FlyBase
- salivary gland morphogenesis Source: FlyBase
- transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Keywordsi
Molecular function | Developmental protein, Kinase, Transferase, Tyrosine-protein kinase |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.10.2 1994 |
SignaLinki | P00528 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Src64B Synonyms:Src1 ORF Names:CG7524 |
Organismi | Drosophila melanogaster (Fruit fly) |
Taxonomic identifieri | 7227 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Ecdysozoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Holometabola › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora › |
Proteomesi |
|
Organism-specific databases
FlyBasei | FBgn0262733 Src64B |
Subcellular locationi
Cytosol
- cytosol Source: FlyBase
Extracellular region or secreted
- germline ring canal Source: FlyBase
Plasma Membrane
- extrinsic component of cytoplasmic side of plasma membrane Source: GO_Central
- plasma membrane Source: FlyBase
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000088140 | 1 – 552 | Tyrosine-protein kinase Src64BAdd BLAST | 552 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 434 | Phosphotyrosine; by autocatalysisBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | P00528 |
PRIDEi | P00528 |
PTM databases
iPTMneti | P00528 |
Expressioni
Tissue specificityi
After the first 8 hours of development, accumulates almost exclusively in neural tissues such as the brain, ventral nerve chord, and eye-antennal disks, and in differentiating smooth muscle.1 Publication
Developmental stagei
Abundant in embryos and pupae, rare in larvae and adults.1 Publication
Gene expression databases
Bgeei | FBgn0262733 Expressed in 40 organ(s), highest expression level in embryo |
ExpressionAtlasi | P00528 baseline and differential |
Genevisiblei | P00528 DM |
Interactioni
Binary interactionsi
GO - Molecular functioni
- signaling receptor binding Source: FlyBase
Protein-protein interaction databases
BioGridi | 71867, 66 interactors |
DIPi | DIP-17438N |
IntActi | P00528, 9 interactors |
STRINGi | 7227.FBpp0293527 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 95 – 156 | SH3PROSITE-ProRule annotationAdd BLAST | 62 | |
Domaini | 162 – 259 | SH2PROSITE-ProRule annotationAdd BLAST | 98 | |
Domaini | 284 – 537 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 254 |
Sequence similaritiesi
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.PROSITE-ProRule annotation
Keywords - Domaini
SH2 domain, SH3 domainPhylogenomic databases
eggNOGi | KOG0197 Eukaryota COG0515 LUCA |
GeneTreei | ENSGT00940000164033 |
InParanoidi | P00528 |
KOi | K05704 |
OMAi | GKWRNNI |
OrthoDBi | 539311at2759 |
PhylomeDBi | P00528 |
Family and domain databases
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR000980 SH2 IPR036860 SH2_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF07714 Pkinase_Tyr, 1 hit PF00017 SH2, 1 hit PF00018 SH3_1, 1 hit |
PRINTSi | PR00401 SH2DOMAIN PR00452 SH3DOMAIN PR00109 TYRKINASE |
SMARTi | View protein in SMART SM00252 SH2, 1 hit SM00326 SH3, 1 hit SM00219 TyrKc, 1 hit |
SUPFAMi | SSF50044 SSF50044, 1 hit SSF55550 SSF55550, 1 hit SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit PS50001 SH2, 1 hit PS50002 SH3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
P00528-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGNKCCSKRQ DQELALAYPT GGYKKSDYTF GQTHINSSGG GNMGGVLGQK
60 70 80 90 100
HNNGGSLDSR YTPDPNHRGP LKIGGKGGVD IIRPRTTPTG VPGVVLKRVV
110 120 130 140 150
VALYDYKSRD ESDLSFMKGD RMEVIDDTES DWWRVVNLTT RQEGLIPLNF
160 170 180 190 200
VAEERSVNSE DWFFENVLRK EADKLLLAEE NPRGTFLVRP SEHNPNGYSL
210 220 230 240 250
SVKDWEDGRG YHVKHYRIKP LDNGGYYIAT NQTFPSLQAL VMAYSKNALG
260 270 280 290 300
LCHILSRPCP KPQPQMWDLG PELRDKYEIP RSEIQLLRKL GRGNFGEVFY
310 320 330 340 350
GKWRNSIDVA VKTLREGTMS TAAFLQEAAI MKKFRHNRLV ALYAVCSQEE
360 370 380 390 400
PIYIVQEYMS KGSLLDFLRE GDGRYLHFED LIYIATQVAS GMEYLESKQL
410 420 430 440 450
IHRDLAARNV LIGENNVAKI CDFGLARVIA DDEYCPKQGS RFPVKWTAPE
460 470 480 490 500
AIIYGKFSIK SDVWSYGILL MELFTYGQVP YPGMHSREVI ENIERGFRMP
510 520 530 540 550
KPTNHYFPDN IYQLLLQCWD AVPEKRPTFE FLNHYFESFS VTSEVPYREV
QD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketM9MSK3 | M9MSK3_DROME | Tyrosine-protein kinase | Src64B c-Src, c-src, C-src1, D-src, D-Src64B | 552 | Annotation score: | ||
X2JCI2 | X2JCI2_DROME | Tyrosine-protein kinase | Src64B c-Src, c-src, C-src1, D-src, D-Src64B | 553 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 102 | A → S in AAA28913 (PubMed:2996778).Curated | 1 | |
Sequence conflicti | 261 – 272 | KPQPQ…DLGPE → ASLPQTAAPDVGFGPQ in AAA28489 (PubMed:6317185).CuratedAdd BLAST | 12 | |
Sequence conflicti | 286 – 287 | LL → VV in AAA28489 (PubMed:6317185).Curated | 2 | |
Sequence conflicti | 290 | L → V in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 293 | G → R in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 316 | E → A in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 366 | D → N in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 373 | G → D in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 384 – 385 | IA → MH in AAA28489 (PubMed:6317185).Curated | 2 | |
Sequence conflicti | 389 – 390 | AS → TT in AAA28489 (PubMed:6317185).Curated | 2 | |
Sequence conflicti | 393 | E → K in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 400 | L → V in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 406 – 407 | AA → TT in AAA28489 (PubMed:6317185).Curated | 2 | |
Sequence conflicti | 435 | C → R in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 460 | K → E in CAA05754 (PubMed:9731193).Curated | 1 | |
Sequence conflicti | 471 | M → T in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 484 | M → L in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 507 | F → L in AAA28489 (PubMed:6317185).Curated | 1 | |
Sequence conflicti | 536 | F → L in AAA28489 (PubMed:6317185).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11917 mRNA Translation: AAA28913.1 AE014296 Genomic DNA Translation: AAF47922.1 AE014296 Genomic DNA Translation: AAX52734.1 AE014296 Genomic DNA Translation: AAX52735.1 AE014296 Genomic DNA Translation: AAX52736.1 AE014296 Genomic DNA Translation: AAX52737.1 AY051781 mRNA Translation: AAK93205.1 K01043 Genomic DNA Translation: AAA28489.1 AJ002919 Genomic DNA Translation: CAA05754.1 |
RefSeqi | NP_001014561.1, NM_001014561.2 NP_001014562.1, NM_001014562.3 NP_001014563.1, NM_001014563.3 NP_001014564.1, NM_001014564.3 NP_001189051.1, NM_001202122.1 NP_001246628.1, NM_001259699.2 NP_001246629.1, NM_001259700.2 NP_524934.2, NM_080195.4 |
Genome annotation databases
EnsemblMetazoai | FBtr0073321; FBpp0073177; FBgn0262733 FBtr0100504; FBpp0099944; FBgn0262733 FBtr0100505; FBpp0099946; FBgn0262733 FBtr0100507; FBpp0099947; FBgn0262733 FBtr0100508; FBpp0099948; FBgn0262733 FBtr0302594; FBpp0291750; FBgn0262733 FBtr0304989; FBpp0293526; FBgn0262733 FBtr0304990; FBpp0293527; FBgn0262733 |
GeneIDi | 48973 |
KEGGi | dme:Dmel_CG7524 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M11917 mRNA Translation: AAA28913.1 AE014296 Genomic DNA Translation: AAF47922.1 AE014296 Genomic DNA Translation: AAX52734.1 AE014296 Genomic DNA Translation: AAX52735.1 AE014296 Genomic DNA Translation: AAX52736.1 AE014296 Genomic DNA Translation: AAX52737.1 AY051781 mRNA Translation: AAK93205.1 K01043 Genomic DNA Translation: AAA28489.1 AJ002919 Genomic DNA Translation: CAA05754.1 |
RefSeqi | NP_001014561.1, NM_001014561.2 NP_001014562.1, NM_001014562.3 NP_001014563.1, NM_001014563.3 NP_001014564.1, NM_001014564.3 NP_001189051.1, NM_001202122.1 NP_001246628.1, NM_001259699.2 NP_001246629.1, NM_001259700.2 NP_524934.2, NM_080195.4 |
3D structure databases
SMRi | P00528 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 71867, 66 interactors |
DIPi | DIP-17438N |
IntActi | P00528, 9 interactors |
STRINGi | 7227.FBpp0293527 |
PTM databases
iPTMneti | P00528 |
Proteomic databases
PaxDbi | P00528 |
PRIDEi | P00528 |
Genome annotation databases
EnsemblMetazoai | FBtr0073321; FBpp0073177; FBgn0262733 FBtr0100504; FBpp0099944; FBgn0262733 FBtr0100505; FBpp0099946; FBgn0262733 FBtr0100507; FBpp0099947; FBgn0262733 FBtr0100508; FBpp0099948; FBgn0262733 FBtr0302594; FBpp0291750; FBgn0262733 FBtr0304989; FBpp0293526; FBgn0262733 FBtr0304990; FBpp0293527; FBgn0262733 |
GeneIDi | 48973 |
KEGGi | dme:Dmel_CG7524 |
Organism-specific databases
CTDi | 48973 |
FlyBasei | FBgn0262733 Src64B |
Phylogenomic databases
eggNOGi | KOG0197 Eukaryota COG0515 LUCA |
GeneTreei | ENSGT00940000164033 |
InParanoidi | P00528 |
KOi | K05704 |
OMAi | GKWRNNI |
OrthoDBi | 539311at2759 |
PhylomeDBi | P00528 |
Enzyme and pathway databases
BRENDAi | 2.7.10.2 1994 |
SignaLinki | P00528 |
Miscellaneous databases
ChiTaRSi | Src64B fly |
GenomeRNAii | 48973 |
PROi | PR:P00528 |
Gene expression databases
Bgeei | FBgn0262733 Expressed in 40 organ(s), highest expression level in embryo |
ExpressionAtlasi | P00528 baseline and differential |
Genevisiblei | P00528 DM |
Family and domain databases
Gene3Di | 3.30.505.10, 1 hit |
InterProi | View protein in InterPro IPR011009 Kinase-like_dom_sf IPR000719 Prot_kinase_dom IPR017441 Protein_kinase_ATP_BS IPR001245 Ser-Thr/Tyr_kinase_cat_dom IPR000980 SH2 IPR036860 SH2_dom_sf IPR036028 SH3-like_dom_sf IPR001452 SH3_domain IPR008266 Tyr_kinase_AS IPR020635 Tyr_kinase_cat_dom |
Pfami | View protein in Pfam PF07714 Pkinase_Tyr, 1 hit PF00017 SH2, 1 hit PF00018 SH3_1, 1 hit |
PRINTSi | PR00401 SH2DOMAIN PR00452 SH3DOMAIN PR00109 TYRKINASE |
SMARTi | View protein in SMART SM00252 SH2, 1 hit SM00326 SH3, 1 hit SM00219 TyrKc, 1 hit |
SUPFAMi | SSF50044 SSF50044, 1 hit SSF55550 SSF55550, 1 hit SSF56112 SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107 PROTEIN_KINASE_ATP, 1 hit PS50011 PROTEIN_KINASE_DOM, 1 hit PS00109 PROTEIN_KINASE_TYR, 1 hit PS50001 SH2, 1 hit PS50002 SH3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SRC64_DROME | |
Accessioni | P00528Primary (citable) accession number: P00528 Secondary accession number(s): A4V1H7, O18372, Q9VZA2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | June 1, 2001 | |
Last modified: | October 16, 2019 | |
This is version 214 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Drosophila annotation project |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - Drosophila
Drosophila: entries, gene names and cross-references to FlyBase