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Protein

cAMP-dependent protein kinase type I-alpha regulatory subunit

Gene

PRKAR1A

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei201cAMP 11
Binding sitei210cAMP 11
Binding sitei325cAMP 21
Binding sitei334cAMP 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi136 – 253cAMP 1Add BLAST118
Nucleotide bindingi254 – 380cAMP 2Add BLAST127

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandcAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-BTA-163615 PKA activation
R-BTA-164378 PKA activation in glucagon signalling
R-BTA-180024 DARPP-32 events
R-BTA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-BTA-5610787 Hedgehog 'off' state
R-BTA-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type I-alpha regulatory subunit
Cleaved into the following chain:
Gene namesi
Name:PRKAR1A
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 19

Organism-specific databases

VGNCiVGNC:33324 PRKAR1A

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004232161 – 380cAMP-dependent protein kinase type I-alpha regulatory subunitAdd BLAST380
Initiator methionineiRemoved; alternate1 Publication
ChainiPRO_00002053762 – 380cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processedAdd BLAST379

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylalanine; in cAMP-dependent protein kinase type I-alpha regulatory subunit, N-terminally processed1 Publication1
Modified residuei3PhosphoserineBy similarity1
Disulfide bondi17Interchain (with C-37)1 Publication
Disulfide bondi38Interchain (with C-16)1 Publication
Modified residuei76PhosphoserineBy similarity1
Modified residuei82PhosphoserineBy similarity1
Modified residuei100PhosphoserineBy similarity1
Modified residuei257PhosphoserineBy similarity1

Post-translational modificationi

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity. The physiological significance of the in vitro phosphorylation of a proximal serine is unclear.

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiP00514
PRIDEiP00514

PTM databases

iPTMnetiP00514

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

BgeeiENSBTAG00000008621 Expressed in 9 organ(s), highest expression level in testis

Interactioni

Subunit structurei

The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. PRKAR1A also interacts with RFC2; the complex may be involved in cell survival. Interacts with AKAP4. Interacts with RARA; the interaction occurs in the presence of cAMP or FSH and regulates RARA transcriptional activity (By similarity). Interacts with the phosphorylated form of PJA2 (By similarity). Interacts with CBFA2T3 (By similarity). Interacts with PRKX; regulates this cAMP-dependent protein kinase (By similarity). Interacts with smAKAP; this interaction may target PRKAR1A to the plasma membrane (By similarity). Interacts with AICDA (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-36644N
IntActiP00514, 3 interactors
MINTiP00514
STRINGi9913.ENSBTAP00000011371

Chemistry databases

BindingDBiP00514

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00245
ProteinModelPortaliP00514
SMRiP00514
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00514

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 135Dimerization and phosphorylationAdd BLAST134

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi95 – 99Pseudophosphorylation motif5

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196669
HOVERGENiHBG002025
InParanoidiP00514
KOiK04739
OMAiVQLCTVR
OrthoDBiEOG091G0F1K
TreeFamiTF314920

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00514-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASGTTASEE ERSLRECELY VQKHNIQALL KDSIVQLCTA RPERPMAFLR
60 70 80 90 100
EYFEKLEKEE AKQIQNLQKA GSRADSREDE ISPPPPNPVV KGRRRRGAIS
110 120 130 140 150
AEVYTEEDAA SYVRKVIPKD YKTMAALAKA IEKNVLFSHL DDNERSDIFD
160 170 180 190 200
AMFPVSFIAG ETVIQQGDEG DNFYVIDQGE MDVYVNNEWA TSVGEGGSFG
210 220 230 240 250
ELALIYGTPR AATVKAKTNV KLWGIDRDSY RRILMGSTLR KRKMYEEFLS
260 270 280 290 300
KVSILESLDK WERLTVADAL EPVQFEDGQK IVVQGEPGDE FFIILEGSAA
310 320 330 340 350
VLQRRSENEE FVEVGRLGPS DYFGEIALLM NRPRAATVVA RGPLKCVKLD
360 370 380
RPRFERVLGP CSDILKRNIQ QYNSFVSLSV
Length:380
Mass (Da):42,893
Last modified:April 17, 2007 - v2
Checksum:iB086A291809422F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti230Y → N in AAA30708 (PubMed:6190178).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT030573 mRNA Translation: ABQ13013.1
BC118242 mRNA Translation: AAI18243.1
K00833 mRNA Translation: AAA30708.1
PIRiA00617 OKBO1R
I45957
RefSeqiNP_001069826.1, NM_001076358.1
UniGeneiBt.10903

Genome annotation databases

EnsembliENSBTAT00000011371; ENSBTAP00000011371; ENSBTAG00000008621
GeneIDi615074
KEGGibta:615074

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT030573 mRNA Translation: ABQ13013.1
BC118242 mRNA Translation: AAI18243.1
K00833 mRNA Translation: AAA30708.1
PIRiA00617 OKBO1R
I45957
RefSeqiNP_001069826.1, NM_001076358.1
UniGeneiBt.10903

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1APKmodel-A2-380[»]
1BPKmodel-A2-380[»]
1NE4X-ray2.40A95-377[»]
1NE6X-ray2.30A95-377[»]
1PVKmodel-A114-245[»]
1RGSX-ray2.80A93-380[»]
1RL3X-ray2.70A/B93-380[»]
2EZWNMR-A/B13-62[»]
2QCSX-ray2.20B91-380[»]
3FHIX-ray2.00B92-245[»]
3IIAX-ray2.70A92-245[»]
3IM3X-ray2.00A13-62[»]
3IM4X-ray2.28A/B13-62[»]
3PLQX-ray2.30A92-245[»]
3PNAX-ray1.50A/B92-245[»]
3PVBX-ray3.30B85-244[»]
4JV4X-ray2.95A93-380[»]
4MX3X-ray3.88A/B2-380[»]
4X6RX-ray2.40B91-380[»]
5HVZX-ray2.00A/B13-62[»]
5JR7X-ray3.56B/D92-366[»]
DisProtiDP00245
ProteinModelPortaliP00514
SMRiP00514
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-36644N
IntActiP00514, 3 interactors
MINTiP00514
STRINGi9913.ENSBTAP00000011371

Chemistry databases

BindingDBiP00514

PTM databases

iPTMnetiP00514

Proteomic databases

PaxDbiP00514
PRIDEiP00514

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011371; ENSBTAP00000011371; ENSBTAG00000008621
GeneIDi615074
KEGGibta:615074

Organism-specific databases

CTDi5573
VGNCiVGNC:33324 PRKAR1A

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196669
HOVERGENiHBG002025
InParanoidiP00514
KOiK04739
OMAiVQLCTVR
OrthoDBiEOG091G0F1K
TreeFamiTF314920

Enzyme and pathway databases

ReactomeiR-BTA-163615 PKA activation
R-BTA-164378 PKA activation in glucagon signalling
R-BTA-180024 DARPP-32 events
R-BTA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-BTA-5610787 Hedgehog 'off' state
R-BTA-983231 Factors involved in megakaryocyte development and platelet production

Miscellaneous databases

EvolutionaryTraceiP00514
PROiPR:P00514

Gene expression databases

BgeeiENSBTAG00000008621 Expressed in 9 organ(s), highest expression level in testis

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiKAP0_BOVIN
AccessioniPrimary (citable) accession number: P00514
Secondary accession number(s): A5D9F4, Q17QP8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 17, 2007
Last modified: October 10, 2018
This is version 170 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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Main funding by: National Institutes of Health

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