UniProtKB - P00507 (AATM_RAT)
Protein
Aspartate aminotransferase, mitochondrial
Gene
Got2
Organism
Rattus norvegicus (Rat)
Status
Functioni
Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). As a member of the malate-aspartate shuttle, it has a key role in the intracellular NAD(H) redox balance. Is important for metabolite exchange between mitochondria and cytosol, and for amino acid metabolism. Facilitates cellular uptake of long-chain free fatty acids (By similarity).By similarity1 Publication
Miscellaneous
In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.
Catalytic activityi
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 65 | Substrate; via amide nitrogenBy similarity | 1 | |
Binding sitei | 162 | SubstrateBy similarity | 1 | |
Binding sitei | 215 | SubstrateBy similarity | 1 | |
Binding sitei | 407 | SubstrateBy similarity | 1 |
GO - Molecular functioni
- amino acid binding Source: RGD
- carboxylic acid binding Source: RGD
- enzyme binding Source: RGD
- identical protein binding Source: RGD
- kynurenine-oxoglutarate transaminase activity Source: UniProtKB-EC
- L-aspartate:2-oxoglutarate aminotransferase activity Source: RGD
- phospholipid binding Source: RGD
- pyridoxal phosphate binding Source: RGD
GO - Biological processi
- 2-oxoglutarate metabolic process Source: UniProtKB
- aspartate biosynthetic process Source: RGD
- aspartate catabolic process Source: RGD
- aspartate metabolic process Source: RGD
- cellular amino acid metabolic process Source: RGD
- dicarboxylic acid metabolic process Source: RGD
- fatty acid transport Source: RGD
- female pregnancy Source: RGD
- glutamate catabolic process to 2-oxoglutarate Source: RGD
- glutamate catabolic process to aspartate Source: RGD
- glutamate metabolic process Source: UniProtKB
- lactation Source: RGD
- oxaloacetate metabolic process Source: RGD
- response to ethanol Source: RGD
- response to insulin Source: RGD
- response to morphine Source: RGD
- response to muscle activity Source: RGD
Keywordsi
Molecular function | Aminotransferase, Transferase |
Biological process | Lipid transport, Transport |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-15069 |
BRENDAi | 2.6.1.1, 5301 |
Reactomei | R-RNO-389661, Glyoxylate metabolism and glycine degradation R-RNO-70263, Gluconeogenesis R-RNO-8963693, Aspartate and asparagine metabolism R-RNO-8964539, Glutamate and glutamine metabolism |
SABIO-RKi | P00507 |
Names & Taxonomyi
Protein namesi | Recommended name: Aspartate aminotransferase, mitochondrial (EC:2.6.1.1, EC:2.6.1.7)Short name: mAspAT Alternative name(s): Fatty acid-binding protein Short name: FABP-1 Glutamate oxaloacetate transaminase 2 Kynurenine aminotransferase 4 Kynurenine aminotransferase IV Kynurenine--oxoglutarate transaminase 4 Kynurenine--oxoglutarate transaminase IV Plasma membrane-associated fatty acid-binding protein Short name: FABPpm Transaminase A |
Gene namesi | Name:Got2 Synonyms:Maat |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 2722, Got2 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix 1 Publication
Plasma membrane
- Cell membrane 1 Publication
Note: Located in the mitochondria of liver, pancreas, spleen, heart, pituitary gland and submandibular gland cells. In kidney, located in the mitochondria, on the cell surface of regions with protrusions in distal tubules, on the apical cell surface of protrusions along the microvilli in cortical collecting ducts, in condensing vacuoles, on the cell surface at cell boundaries of adjoining kidney cells and on the cell surface of endothelial cells lining capillaries in the glomerulus. Also located at the cell surface of endothelial cells lining arterioles and on the cell surface of lymphocytes.
Mitochondrion
- mitochondrial inner membrane Source: RGD
- mitochondrial matrix Source: Reactome
- mitochondrion Source: RGD
Plasma Membrane
- plasma membrane Source: RGD
- sarcolemma Source: RGD
- T-tubule Source: RGD
Other locations
- cell surface Source: RGD
- perikaryon Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
Cell membrane, Membrane, MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 29 | Mitochondrion4 PublicationsAdd BLAST | 29 | |
ChainiPRO_0000001218 | 30 – 430 | Aspartate aminotransferase, mitochondrialAdd BLAST | 401 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 48 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 59 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 73 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 73 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 82 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 90 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 90 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 96 | 3'-nitrotyrosine; alternateBy similarity | 1 | |
Modified residuei | 96 | Phosphotyrosine; alternateBy similarity | 1 | |
Modified residuei | 107 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 107 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 122 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 122 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 143 | PhosphoserineBy similarity | 1 | |
Modified residuei | 159 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 159 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 185 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 185 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 227 | N6-succinyllysineBy similarity | 1 | |
Modified residuei | 234 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 279 | N6-(pyridoxal phosphate)lysine; alternate | 1 | |
Modified residuei | 279 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 296 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 296 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 302 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 309 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 309 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 313 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 338 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 338 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 345 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 363 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 363 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 364 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 387 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 396 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 396 | N6-succinyllysine; alternateBy similarity | 1 | |
Modified residuei | 404 | N6-acetyllysine; alternateBy similarity | 1 | |
Modified residuei | 404 | N6-succinyllysine; alternateBy similarity | 1 |
Keywords - PTMi
Acetylation, Methylation, Nitration, PhosphoproteinProteomic databases
jPOSTi | P00507 |
PaxDbi | P00507 |
PRIDEi | P00507 |
PTM databases
CarbonylDBi | P00507 |
iPTMneti | P00507 |
PhosphoSitePlusi | P00507 |
Expressioni
Tissue specificityi
Expressed in all tissues tested: liver, pancreas, kidney, heart, spleen, arterioles, and lymphocytes.1 Publication
Gene expression databases
Bgeei | ENSRNOG00000011782, Expressed in heart and 21 other tissues |
Genevisiblei | P00507, RN |
Interactioni
Subunit structurei
Homodimer.
GO - Molecular functioni
- enzyme binding Source: RGD
- identical protein binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 247750, 3 interactors |
IntActi | P00507, 4 interactors |
STRINGi | 10116.ENSRNOP00000015956 |
Family & Domainsi
Sequence similaritiesi
Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1411, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P00507 |
OMAi | VGACTIV |
OrthoDBi | 1104596at2759 |
PhylomeDBi | P00507 |
TreeFami | TF300641 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00507-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MALLHSGRVL SGMAAAFHPG LAAAASARAS SWWTHVEMGP PDPILGVTEA
60 70 80 90 100
FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAGK NLDKEYLPIG
110 120 130 140 150
GLADFCKASA ELALGENSEV LKSGRFVTVQ TISGTGALRV GASFLQRFFK
160 170 180 190 200
FSRDVFLPKP SWGNHTPIFR DAGMQLQGYR YYDPKTCGFD FSGALEDISK
210 220 230 240 250
IPEQSVLLLH ACAHNPTGVD PRPEQWKEMA AVVKKKNLFA FFDMAYQGFA
260 270 280 290 300
SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTVVCKDAEE
310 320 330 340 350
AKRVESQLKI LIRPLYSNPP LNGARIAATI LTSPDLRKQW LQEVKGMADR
360 370 380 390 400
IISMRTQLVS NLKKEGSSHN WQHITDQIGM FCFTGLKPEQ VERLTKEFSV
410 420 430
YMTKDGRISV AGVTSGNVGY LAHAIHQVTK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 30 | S → R in AAC13868 (PubMed:7759512).Curated | 1 | |
Sequence conflicti | 162 | W → G AA sequence (PubMed:7470110).Curated | 1 | |
Sequence conflicti | 162 | W → G AA sequence (PubMed:7309704).Curated | 1 | |
Sequence conflicti | 167 – 169 | PIF → EIA AA sequence (PubMed:7470110).Curated | 3 | |
Sequence conflicti | 167 – 169 | PIF → EIA AA sequence (PubMed:7309704).Curated | 3 | |
Sequence conflicti | 177 | Q → E AA sequence (PubMed:7470110).Curated | 1 | |
Sequence conflicti | 177 | Q → E AA sequence (PubMed:7309704).Curated | 1 | |
Sequence conflicti | 232 | V → Y AA sequence (PubMed:7470110).Curated | 1 | |
Sequence conflicti | 232 | V → Y AA sequence (PubMed:7309704).Curated | 1 | |
Sequence conflicti | 255 | D → N AA sequence (PubMed:7470110).Curated | 1 | |
Sequence conflicti | 255 | D → N AA sequence (PubMed:7309704).Curated | 1 | |
Sequence conflicti | 338 – 339 | KQ → QG AA sequence (PubMed:7470110).Curated | 2 | |
Sequence conflicti | 338 – 339 | KQ → QG AA sequence (PubMed:7309704).Curated | 2 | |
Sequence conflicti | 352 | I → G AA sequence (PubMed:7470110).Curated | 1 | |
Sequence conflicti | 352 | I → G AA sequence (PubMed:7309704).Curated | 1 | |
Sequence conflicti | 386 | L → I AA sequence (PubMed:7470110).Curated | 1 | |
Sequence conflicti | 386 | L → I AA sequence (PubMed:7309704).Curated | 1 | |
Sequence conflicti | 400 | V → I AA sequence (PubMed:7470110).Curated | 1 | |
Sequence conflicti | 400 | V → I AA sequence (PubMed:7309704).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18467 mRNA Translation: AAB54275.1 BC061792 mRNA Translation: AAH61792.1 U21158 Genomic DNA Translation: AAC13868.1 M12709 mRNA Translation: AAA41267.1 |
PIRi | A28005, XNRTDM I55427 |
RefSeqi | NP_037309.1, NM_013177.2 |
Genome annotation databases
Ensembli | ENSRNOT00000015956; ENSRNOP00000015956; ENSRNOG00000011782 |
GeneIDi | 25721 |
KEGGi | rno:25721 |
UCSCi | RGD:2722, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M18467 mRNA Translation: AAB54275.1 BC061792 mRNA Translation: AAH61792.1 U21158 Genomic DNA Translation: AAC13868.1 M12709 mRNA Translation: AAA41267.1 |
PIRi | A28005, XNRTDM I55427 |
RefSeqi | NP_037309.1, NM_013177.2 |
3D structure databases
SMRi | P00507 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 247750, 3 interactors |
IntActi | P00507, 4 interactors |
STRINGi | 10116.ENSRNOP00000015956 |
Chemistry databases
ChEMBLi | CHEMBL2351 |
PTM databases
CarbonylDBi | P00507 |
iPTMneti | P00507 |
PhosphoSitePlusi | P00507 |
Proteomic databases
jPOSTi | P00507 |
PaxDbi | P00507 |
PRIDEi | P00507 |
Genome annotation databases
Ensembli | ENSRNOT00000015956; ENSRNOP00000015956; ENSRNOG00000011782 |
GeneIDi | 25721 |
KEGGi | rno:25721 |
UCSCi | RGD:2722, rat |
Organism-specific databases
CTDi | 2806 |
RGDi | 2722, Got2 |
Phylogenomic databases
eggNOGi | KOG1411, Eukaryota |
GeneTreei | ENSGT00950000183082 |
HOGENOMi | CLU_032440_1_2_1 |
InParanoidi | P00507 |
OMAi | VGACTIV |
OrthoDBi | 1104596at2759 |
PhylomeDBi | P00507 |
TreeFami | TF300641 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER-15069 |
BRENDAi | 2.6.1.1, 5301 |
Reactomei | R-RNO-389661, Glyoxylate metabolism and glycine degradation R-RNO-70263, Gluconeogenesis R-RNO-8963693, Aspartate and asparagine metabolism R-RNO-8964539, Glutamate and glutamine metabolism |
SABIO-RKi | P00507 |
Miscellaneous databases
PROi | PR:P00507 |
Gene expression databases
Bgeei | ENSRNOG00000011782, Expressed in heart and 21 other tissues |
Genevisiblei | P00507, RN |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
InterProi | View protein in InterPro IPR004839, Aminotransferase_I/II IPR000796, Asp_trans IPR004838, NHTrfase_class1_PyrdxlP-BS IPR015424, PyrdxlP-dep_Trfase IPR015422, PyrdxlP-dep_Trfase_dom1 IPR015421, PyrdxlP-dep_Trfase_major |
PANTHERi | PTHR11879, PTHR11879, 1 hit |
Pfami | View protein in Pfam PF00155, Aminotran_1_2, 1 hit |
PRINTSi | PR00799, TRANSAMINASE |
SUPFAMi | SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS00105, AA_TRANSFER_CLASS_1, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AATM_RAT | |
Accessioni | P00507Primary (citable) accession number: P00507 Secondary accession number(s): Q64551, Q9QV50 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 1, 1989 | |
Last modified: | December 2, 2020 | |
This is version 175 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families