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Protein

Aspartate aminotransferase, mitochondrial

Gene

GOT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids.1 Publication

Miscellaneous

In eukaryotes there are cytoplasmic, mitochondrial and chloroplastic isozymes.

Catalytic activityi

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate.1 Publication
L-kynurenine + 2-oxoglutarate = 4-(2-aminophenyl)-2,4-dioxobutanoate + L-glutamate.

Cofactori

pH dependencei

Optimum pH is 8.5.1 Publication

Temperature dependencei

Optimum temperature is 47.5 degrees Celsius.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65Substrate; via amide nitrogenBy similarity1
Binding sitei162SubstrateBy similarity1
Binding sitei215SubstrateBy similarity1
Binding sitei407SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase
Biological processLipid transport, Transport
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:HS04858-MONOMER
ReactomeiR-HSA-1614558 Degradation of cysteine and homocysteine
R-HSA-389661 Glyoxylate metabolism and glycine degradation
R-HSA-70263 Gluconeogenesis
R-HSA-70614 Amino acid synthesis and interconversion (transamination)

Protein family/group databases

TCDBi9.A.70.1.1 the aspartate amino transferase (aat) family

Names & Taxonomyi

Protein namesi
Recommended name:
Aspartate aminotransferase, mitochondrial (EC:2.6.1.11 Publication, EC:2.6.1.7)
Short name:
mAspAT
Alternative name(s):
Fatty acid-binding protein
Short name:
FABP-1
Glutamate oxaloacetate transaminase 2
Kynurenine aminotransferase 4
Kynurenine aminotransferase IV
Kynurenine--oxoglutarate transaminase 4
Kynurenine--oxoglutarate transaminase IV
Plasma membrane-associated fatty acid-binding protein
Short name:
FABPpm
Transaminase A
Gene namesi
Name:GOT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

EuPathDBiHostDB:ENSG00000125166.12
HGNCiHGNC:4433 GOT2
MIMi138150 gene
neXtProtiNX_P00505

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi2806
OpenTargetsiENSG00000125166
PharmGKBiPA28818

Chemistry databases

DrugBankiDB02783 4'-Deoxy-4'-Acetylyamino-Pyridoxal-5'-Phosphate
DB00128 L-Aspartic Acid
DB00142 L-Glutamic Acid
DB00114 Pyridoxal Phosphate

Polymorphism and mutation databases

BioMutaiGOT2
DMDMi308153643

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 29MitochondrionCombined sources1 PublicationAdd BLAST29
ChainiPRO_000000121530 – 430Aspartate aminotransferase, mitochondrialAdd BLAST401

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei48PhosphothreonineCombined sources1
Modified residuei59N6-acetyllysineBy similarity1
Modified residuei73N6-acetyllysine; alternateCombined sources1
Modified residuei73N6-succinyllysine; alternateBy similarity1
Modified residuei82N6-acetyllysineBy similarity1
Modified residuei90N6-acetyllysine; alternateCombined sources1
Modified residuei90N6-succinyllysine; alternateBy similarity1
Modified residuei96Nitrated tyrosine; alternateBy similarity1
Modified residuei96Phosphotyrosine; alternateCombined sources1
Modified residuei107N6-acetyllysine; alternateBy similarity1
Modified residuei107N6-succinyllysine; alternateBy similarity1
Modified residuei122N6-acetyllysine; alternateBy similarity1
Modified residuei122N6-succinyllysine; alternateBy similarity1
Modified residuei143PhosphoserineCombined sources1
Modified residuei159N6-acetyllysine; alternateCombined sources1
Modified residuei159N6-succinyllysine; alternateBy similarity1
Modified residuei185N6-acetyllysine; alternateBy similarity1
Modified residuei185N6-succinyllysine; alternateBy similarity1
Modified residuei227N6-succinyllysineBy similarity1
Modified residuei234N6-acetyllysineCombined sources1
Modified residuei279N6-(pyridoxal phosphate)lysine; alternateBy similarity1
Modified residuei279N6-acetyllysine; alternateBy similarity1
Modified residuei296N6-acetyllysine; alternateCombined sources1
Modified residuei296N6-succinyllysine; alternateBy similarity1
Modified residuei302N6-acetyllysineBy similarity1
Modified residuei309N6-acetyllysine; alternateBy similarity1
Modified residuei309N6-succinyllysine; alternateBy similarity1
Modified residuei313Asymmetric dimethylarginineBy similarity1
Modified residuei333PhosphothreonineCombined sources1
Modified residuei338N6-acetyllysine; alternateBy similarity1
Modified residuei338N6-succinyllysine; alternateBy similarity1
Modified residuei345N6-acetyllysineBy similarity1
Modified residuei363N6-acetyllysine; alternateBy similarity1
Modified residuei363N6-succinyllysine; alternateBy similarity1
Modified residuei364N6-acetyllysineBy similarity1
Modified residuei387N6-acetyllysineBy similarity1
Modified residuei396N6-acetyllysine; alternateCombined sources1
Modified residuei396N6-succinyllysine; alternateBy similarity1
Modified residuei404N6-acetyllysine; alternateCombined sources1
Modified residuei404N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation, Methylation, Nitration, Phosphoprotein

Proteomic databases

EPDiP00505
MaxQBiP00505
PaxDbiP00505
PeptideAtlasiP00505
PRIDEiP00505
ProteomicsDBi51258
TopDownProteomicsiP00505-1 [P00505-1]

2D gel databases

UCD-2DPAGEiP00505

PTM databases

iPTMnetiP00505
PhosphoSitePlusiP00505
SwissPalmiP00505

Expressioni

Inductioni

Up-regulated by long-time exposure to alcohol.1 Publication

Gene expression databases

BgeeiENSG00000125166 Expressed in 231 organ(s), highest expression level in vastus lateralis
CleanExiHS_GOT2
ExpressionAtlasiP00505 baseline and differential
GenevisibleiP00505 HS

Organism-specific databases

HPAiHPA018139

Interactioni

Subunit structurei

Homodimer.

GO - Molecular functioni

Protein-protein interaction databases

BioGridi109068, 22 interactors
IntActiP00505, 10 interactors
MINTiP00505
STRINGi9606.ENSP00000245206

Structurei

Secondary structure

1430
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00505
SMRiP00505
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1411 Eukaryota
COG1448 LUCA
GeneTreeiENSGT00390000014081
HOGENOMiHOG000185898
HOVERGENiHBG000951
InParanoidiP00505
KOiK14455
OMAiRVGACTI
OrthoDBiEOG091G06G3
PhylomeDBiP00505
TreeFamiTF300641

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR000796 Asp_trans
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11879 PTHR11879, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PRINTSiPR00799 TRANSAMINASE
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: P00505-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MALLHSGRVL PGIAAAFHPG LAAAASARAS SWWTHVEMGP PDPILGVTEA
60 70 80 90 100
FKRDTNSKKM NLGVGAYRDD NGKPYVLPSV RKAEAQIAAK NLDKEYLPIG
110 120 130 140 150
GLAEFCKASA ELALGENSEV LKSGRFVTVQ TISGTGALRI GASFLQRFFK
160 170 180 190 200
FSRDVFLPKP TWGNHTPIFR DAGMQLQGYR YYDPKTCGFD FTGAVEDISK
210 220 230 240 250
IPEQSVLLLH ACAHNPTGVD PRPEQWKEIA TVVKKRNLFA FFDMAYQGFA
260 270 280 290 300
SGDGDKDAWA VRHFIEQGIN VCLCQSYAKN MGLYGERVGA FTMVCKDADE
310 320 330 340 350
AKRVESQLKI LIRPMYSNPP LNGARIAAAI LNTPDLRKQW LQEVKVMADR
360 370 380 390 400
IIGMRTQLVS NLKKEGSTHN WQHITDQIGM FCFTGLKPEQ VERLIKEFSI
410 420 430
YMTKDGRISV AGVTSSNVGY LAHAIHQVTK
Length:430
Mass (Da):47,518
Last modified:October 5, 2010 - v3
Checksum:iF559567ABF2DB346
GO
Isoform 2 (identifier: P00505-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     83-125: Missing.

Show »
Length:387
Mass (Da):43,030
Checksum:iFF09030A2B5C4E62
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
H3BRE7H3BRE7_HUMAN
Aspartate aminotransferase, mitocho...
GOT2
59Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti110 – 111AE → EA AA sequence (PubMed:4052435).Curated2
Sequence conflicti255D → N AA sequence (PubMed:4052435).Curated1
Sequence conflicti258A → T in BAD96991 (Ref. 3) Curated1
Sequence conflicti305E → Q AA sequence (PubMed:4052435).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0554942A → S. Corresponds to variant dbSNP:rs11558171Ensembl.1
Natural variantiVAR_031710188G → S. Corresponds to variant dbSNP:rs11076256Ensembl.1
Natural variantiVAR_031711346V → G3 PublicationsCorresponds to variant dbSNP:rs30842Ensembl.1
Natural variantiVAR_031712428V → A1 PublicationCorresponds to variant dbSNP:rs17849335Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05484883 – 125Missing in isoform 2. 1 PublicationAdd BLAST43

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22632 mRNA Translation: AAA35568.1
AK295993 mRNA Translation: BAG58768.1
AK223271 mRNA Translation: BAD96991.1
AC012183 Genomic DNA No translation available.
BC000525 mRNA Translation: AAH00525.1
CCDSiCCDS10801.1 [P00505-1]
CCDS67045.1 [P00505-2]
PIRiA31873 XNHUDM
RefSeqiNP_001273149.1, NM_001286220.1 [P00505-2]
NP_002071.2, NM_002080.3 [P00505-1]
UniGeneiHs.599470

Genome annotation databases

EnsembliENST00000245206; ENSP00000245206; ENSG00000125166 [P00505-1]
ENST00000434819; ENSP00000394100; ENSG00000125166 [P00505-2]
GeneIDi2806
KEGGihsa:2806
UCSCiuc002eof.2 human [P00505-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22632 mRNA Translation: AAA35568.1
AK295993 mRNA Translation: BAG58768.1
AK223271 mRNA Translation: BAD96991.1
AC012183 Genomic DNA No translation available.
BC000525 mRNA Translation: AAH00525.1
CCDSiCCDS10801.1 [P00505-1]
CCDS67045.1 [P00505-2]
PIRiA31873 XNHUDM
RefSeqiNP_001273149.1, NM_001286220.1 [P00505-2]
NP_002071.2, NM_002080.3 [P00505-1]
UniGeneiHs.599470

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5AX8X-ray2.99A/B/C/D30-430[»]
ProteinModelPortaliP00505
SMRiP00505
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109068, 22 interactors
IntActiP00505, 10 interactors
MINTiP00505
STRINGi9606.ENSP00000245206

Chemistry databases

DrugBankiDB02783 4'-Deoxy-4'-Acetylyamino-Pyridoxal-5'-Phosphate
DB00128 L-Aspartic Acid
DB00142 L-Glutamic Acid
DB00114 Pyridoxal Phosphate

Protein family/group databases

TCDBi9.A.70.1.1 the aspartate amino transferase (aat) family

PTM databases

iPTMnetiP00505
PhosphoSitePlusiP00505
SwissPalmiP00505

Polymorphism and mutation databases

BioMutaiGOT2
DMDMi308153643

2D gel databases

UCD-2DPAGEiP00505

Proteomic databases

EPDiP00505
MaxQBiP00505
PaxDbiP00505
PeptideAtlasiP00505
PRIDEiP00505
ProteomicsDBi51258
TopDownProteomicsiP00505-1 [P00505-1]

Protocols and materials databases

DNASUi2806
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000245206; ENSP00000245206; ENSG00000125166 [P00505-1]
ENST00000434819; ENSP00000394100; ENSG00000125166 [P00505-2]
GeneIDi2806
KEGGihsa:2806
UCSCiuc002eof.2 human [P00505-1]

Organism-specific databases

CTDi2806
DisGeNETi2806
EuPathDBiHostDB:ENSG00000125166.12
GeneCardsiGOT2
H-InvDBiHIX0013095
HGNCiHGNC:4433 GOT2
HPAiHPA018139
MIMi138150 gene
neXtProtiNX_P00505
OpenTargetsiENSG00000125166
PharmGKBiPA28818
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG1411 Eukaryota
COG1448 LUCA
GeneTreeiENSGT00390000014081
HOGENOMiHOG000185898
HOVERGENiHBG000951
InParanoidiP00505
KOiK14455
OMAiRVGACTI
OrthoDBiEOG091G06G3
PhylomeDBiP00505
TreeFamiTF300641

Enzyme and pathway databases

BioCyciMetaCyc:HS04858-MONOMER
ReactomeiR-HSA-1614558 Degradation of cysteine and homocysteine
R-HSA-389661 Glyoxylate metabolism and glycine degradation
R-HSA-70263 Gluconeogenesis
R-HSA-70614 Amino acid synthesis and interconversion (transamination)

Miscellaneous databases

ChiTaRSiGOT2 human
GeneWikiiGOT2
GenomeRNAii2806
PROiPR:P00505
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000125166 Expressed in 231 organ(s), highest expression level in vastus lateralis
CleanExiHS_GOT2
ExpressionAtlasiP00505 baseline and differential
GenevisibleiP00505 HS

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 2 hits
InterProiView protein in InterPro
IPR004839 Aminotransferase_I/II
IPR000796 Asp_trans
IPR004838 NHTrfase_class1_PyrdxlP-BS
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11879 PTHR11879, 1 hit
PfamiView protein in Pfam
PF00155 Aminotran_1_2, 1 hit
PRINTSiPR00799 TRANSAMINASE
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00105 AA_TRANSFER_CLASS_1, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAATM_HUMAN
AccessioniPrimary (citable) accession number: P00505
Secondary accession number(s): B4DJA6
, E7ERW2, Q53FL3, Q9BWA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 5, 2010
Last modified: September 12, 2018
This is version 200 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  2. SIMILARITY comments
    Index of protein domains and families
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  6. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
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