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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

Hprt1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine-guanine phosphoribosyltransferase (Hprt1), Hypoxanthine phosphoribosyltransferase (Hprt)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69GMPBy similarity1
Active sitei138Proton acceptorBy similarity1
Binding sitei166GMPBy similarity1
Metal bindingi194MagnesiumBy similarity1
Binding sitei194GMP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 142GMPBy similarity9
Nucleotide bindingi186 – 188GMPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
Biological processPurine salvage
LigandMagnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-74217 Purine salvage
UniPathwayi
UPA00591;UER00648

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Alternative name(s):
HPRT B
Gene namesi
Name:Hprt1
Synonyms:Hprt
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:96217 Hprt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3243916

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001395881 – 218Hypoxanthine-guanine phosphoribosyltransferaseAdd BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103N6-acetyllysineCombined sources1
Cross-linki115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateBy similarity
Cross-linki115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei142PhosphothreonineBy similarity1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP00493
MaxQBiP00493
PaxDbiP00493
PeptideAtlasiP00493
PRIDEiP00493

PTM databases

iPTMnetiP00493
PhosphoSitePlusiP00493
SwissPalmiP00493

Expressioni

Gene expression databases

BgeeiENSMUSG00000025630 Expressed in 336 organ(s), highest expression level in morula
CleanExiMM_HPRT1
GenevisibleiP00493 MM

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200411, 1 interactor
DIPiDIP-6034N
MINTiP00493
STRINGi10090.ENSMUSP00000026723

Structurei

3D structure databases

ProteinModelPortaliP00493
SMRiP00493
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3367 Eukaryota
COG0634 LUCA
GeneTreeiENSGT00390000017323
HOGENOMiHOG000236521
HOVERGENiHBG000242
InParanoidiP00493
KOiK00760
OMAiTMDWMAV
OrthoDBiEOG091G0K6K
PhylomeDBiP00493
TreeFamiTF313367

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
InterProiView protein in InterPro
IPR005904 Hxn_phspho_trans
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01203 HGPRTase, 1 hit
PROSITEiView protein in PROSITE
PS00103 PUR_PYR_PR_TRANSFER, 1 hit

Sequencei

Sequence statusi: Complete.

P00493-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPTRSPSVVI SDDEPGYDLD LFCIPNHYAE DLEKVFIPHG LIMDRTERLA
60 70 80 90 100
RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI
110 120 130 140 150
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV
160 170 180 190 200
KQYSPKMVKV ASLLVKRTSR SVGYRPDFVG FEIPDKFVVG YALDYNEYFR
210
DLNHVCVISE TGKAKYKA
Length:218
Mass (Da):24,570
Last modified:January 23, 2007 - v3
Checksum:i925CC0D4A6626E05
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti201D → N in AAA96232 (PubMed:6294614).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00423 mRNA Translation: AAA96232.1
K01515
, K01507, K01508, K01509, K01510, K01511, K01512, K01513, K01514 Genomic DNA Translation: AAA96271.1
AK002286 mRNA Translation: BAB21989.1
AK088114 mRNA Translation: BAC40153.1
AK146626 mRNA Translation: BAE27315.1
BC083145 mRNA Translation: AAH83145.1
CCDSiCCDS40972.1
PIRiI49756 RTMSG
RefSeqiNP_038584.2, NM_013556.2
UniGeneiMm.299381

Genome annotation databases

EnsembliENSMUST00000026723; ENSMUSP00000026723; ENSMUSG00000025630
GeneIDi15452
KEGGimmu:15452
UCSCiuc009ter.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J00423 mRNA Translation: AAA96232.1
K01515
, K01507, K01508, K01509, K01510, K01511, K01512, K01513, K01514 Genomic DNA Translation: AAA96271.1
AK002286 mRNA Translation: BAB21989.1
AK088114 mRNA Translation: BAC40153.1
AK146626 mRNA Translation: BAE27315.1
BC083145 mRNA Translation: AAH83145.1
CCDSiCCDS40972.1
PIRiI49756 RTMSG
RefSeqiNP_038584.2, NM_013556.2
UniGeneiMm.299381

3D structure databases

ProteinModelPortaliP00493
SMRiP00493
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200411, 1 interactor
DIPiDIP-6034N
MINTiP00493
STRINGi10090.ENSMUSP00000026723

Chemistry databases

ChEMBLiCHEMBL3243916

PTM databases

iPTMnetiP00493
PhosphoSitePlusiP00493
SwissPalmiP00493

Proteomic databases

EPDiP00493
MaxQBiP00493
PaxDbiP00493
PeptideAtlasiP00493
PRIDEiP00493

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000026723; ENSMUSP00000026723; ENSMUSG00000025630
GeneIDi15452
KEGGimmu:15452
UCSCiuc009ter.1 mouse

Organism-specific databases

CTDi15452
MGIiMGI:96217 Hprt

Phylogenomic databases

eggNOGiKOG3367 Eukaryota
COG0634 LUCA
GeneTreeiENSGT00390000017323
HOGENOMiHOG000236521
HOVERGENiHBG000242
InParanoidiP00493
KOiK00760
OMAiTMDWMAV
OrthoDBiEOG091G0K6K
PhylomeDBiP00493
TreeFamiTF313367

Enzyme and pathway databases

UniPathwayi
UPA00591;UER00648

ReactomeiR-MMU-74217 Purine salvage

Miscellaneous databases

ChiTaRSiHprt mouse
PROiPR:P00493
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000025630 Expressed in 336 organ(s), highest expression level in morula
CleanExiMM_HPRT1
GenevisibleiP00493 MM

Family and domain databases

CDDicd06223 PRTases_typeI, 1 hit
InterProiView protein in InterPro
IPR005904 Hxn_phspho_trans
IPR000836 PRibTrfase_dom
IPR029057 PRTase-like
PfamiView protein in Pfam
PF00156 Pribosyltran, 1 hit
SUPFAMiSSF53271 SSF53271, 1 hit
TIGRFAMsiTIGR01203 HGPRTase, 1 hit
PROSITEiView protein in PROSITE
PS00103 PUR_PYR_PR_TRANSFER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiHPRT_MOUSE
AccessioniPrimary (citable) accession number: P00493
Secondary accession number(s): Q545Y2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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