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Protein

Ornithine carbamoyltransferase, mitochondrial

Gene

Otc

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Activity regulationi

Negatively regulated by lysine acetylation.By similarity

Pathwayi: urea cycle

This protein is involved in step 1 of the subpathway that synthesizes L-citrulline from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in this subpathway in this organism are:
  1. Ornithine carbamoyltransferase, mitochondrial (Otc)
This subpathway is part of the pathway urea cycle, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-citrulline from L-ornithine and carbamoyl phosphate, the pathway urea cycle and in Nitrogen metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei141Carbamoyl phosphateBy similarity1
Binding sitei141OrnithineBy similarity1
Binding sitei168Carbamoyl phosphateBy similarity1
Binding sitei199OrnithineBy similarity1
Active sitei303By similarity1
Binding sitei330Carbamoyl phosphateBy similarity1
Binding sitei330OrnithineBy similarity1

GO - Molecular functioni

  • amino acid binding Source: RGD
  • ornithine carbamoyltransferase activity Source: RGD
  • phosphate ion binding Source: RGD
  • phospholipid binding Source: RGD

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Arginine biosynthesis, Urea cycle

Enzyme and pathway databases

ReactomeiR-RNO-1268020 Mitochondrial protein import
R-RNO-70635 Urea cycle
SABIO-RKiP00481
UniPathwayi
UPA00158;UER00271

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase, mitochondrial (EC:2.1.3.3)
Alternative name(s):
Ornithine transcarbamylase
Short name:
OTCase
Gene namesi
Name:Otc
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi3236 Otc

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi92R → L: Strong decrease in activity. 1 Publication1
Mutagenesisi303C → S: Increases Km for ornithine 5-fold and decreases Kcat 20-fold. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32Mitochondrion1 PublicationAdd BLAST32
ChainiPRO_000002033933 – 354Ornithine carbamoyltransferase, mitochondrialAdd BLAST322

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei70N6-acetyllysine; alternateBy similarity1
Modified residuei70N6-succinyllysine; alternateBy similarity1
Modified residuei80N6-succinyllysineBy similarity1
Modified residuei88N6-acetyllysine; alternateBy similarity1
Modified residuei88N6-succinyllysine; alternateBy similarity1
Modified residuei133PhosphoserineBy similarity1
Modified residuei144N6-acetyllysine; alternateBy similarity1
Modified residuei144N6-succinyllysine; alternateBy similarity1
Modified residuei221N6-acetyllysine; alternateBy similarity1
Modified residuei221N6-succinyllysine; alternateBy similarity1
Modified residuei231N6-acetyllysine; alternateBy similarity1
Modified residuei231N6-succinyllysine; alternateBy similarity1
Modified residuei238N6-acetyllysine; alternateBy similarity1
Modified residuei238N6-succinyllysine; alternateBy similarity1
Modified residuei274N6-succinyllysineBy similarity1
Modified residuei289N6-succinyllysineBy similarity1
Modified residuei292N6-acetyllysine; alternateBy similarity1
Modified residuei292N6-succinyllysine; alternateBy similarity1
Modified residuei307N6-acetyllysine; alternateBy similarity1
Modified residuei307N6-succinyllysine; alternateBy similarity1

Post-translational modificationi

Acetylation at Lys-88 negatively regulates ornithine carbamoyltransferase activity in response to nutrient signals.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00481
PRIDEiP00481

PTM databases

iPTMnetiP00481
PhosphoSitePlusiP00481

Expressioni

Gene expression databases

BgeeiENSRNOG00000003370 Expressed in 6 organ(s), highest expression level in liver
GenevisibleiP00481 RN

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004686

Structurei

3D structure databases

ProteinModelPortaliP00481
SMRiP00481
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni90 – 94Ornithine and carbamoyl phosphate bindingBy similarity5
Regioni168 – 171Ornithine and carbamoyl phosphate bindingBy similarity4
Regioni263 – 267Ornithine bindingBy similarity5
Regioni302 – 305Ornithine bindingBy similarity4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1504 Eukaryota
COG0078 LUCA
GeneTreeiENSGT00510000047417
HOGENOMiHOG000022686
HOVERGENiHBG007881
InParanoidiP00481
KOiK00611
OMAiDGNNVCN
OrthoDBiEOG091G0QB1
PhylomeDBiP00481
TreeFamiTF352580

Family and domain databases

Gene3Di3.40.50.1370, 2 hits
InterProiView protein in InterPro
IPR006132 Asp/Orn_carbamoyltranf_P-bd
IPR006130 Asp/Orn_carbamoylTrfase
IPR036901 Asp/Orn_carbamoylTrfase_sf
IPR006131 Asp_carbamoyltransf_Asp/Orn-bd
IPR002292 Orn/put_carbamltrans
PfamiView protein in Pfam
PF00185 OTCace, 1 hit
PF02729 OTCace_N, 1 hit
PRINTSiPR00100 AOTCASE
PR00102 OTCASE
SUPFAMiSSF53671 SSF53671, 1 hit
TIGRFAMsiTIGR00658 orni_carb_tr, 1 hit
PROSITEiView protein in PROSITE
PS00097 CARBAMOYLTRANSFERASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00481-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MLSNLRILLN KAALRKAHTS MVRNFRYGKP VQSQVQLKGR DLLTLKNFTG
60 70 80 90 100
EEIQYMLWLS ADLKFRIKQK GEYLPLLQGK SLGMIFEKRS TRTRLSTETG
110 120 130 140 150
FALLGGHPSF LTTQDIHLGV NESLTDTARV LSSMTDAVLA RVYKQSDLDI
160 170 180 190 200
LAKEATIPIV NGLSDLYHPI QILADYLTLQ EHYGSLKGLT LSWIGDGNNI
210 220 230 240 250
LHSIMMSAAK FGMHLQAATP KGYEPDPNIV KLAEQYAKEN GTRLSMTNDP
260 270 280 290 300
LEAARGGNVL ITDTWISMGQ EDEKKKRLQA FQGYQVTMKT AKVAASDWTF
310 320 330 340 350
LHCLPRKPEE VDDEVFYSPR SLVFPEAENR KWTIMAVMVS LLTDYSPVLQ

KPKF
Length:354
Mass (Da):39,886
Last modified:July 21, 1986 - v1
Checksum:i156B511AF7063F0C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti39G → P in AAA41772 (PubMed:3839075).Curated1
Sequence conflicti241G → S in CAA26007 (PubMed:3839075).Curated1
Sequence conflicti241G → S in AAA41772 (PubMed:3839075).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03040 mRNA Translation: AAA41768.1
M16933
, M16924, M16925, M16926, M16928, M16929, M16930, M16932 Genomic DNA Translation: AAA41769.1
X01976 mRNA Translation: CAA26007.1
K00001 mRNA Translation: AAA41772.1
M11266 mRNA Translation: AAA41767.1
X01178 mRNA Translation: CAA25618.1
PIRiA00563 OWRT
RefSeqiNP_037210.1, NM_013078.1
UniGeneiRn.2391

Genome annotation databases

EnsembliENSRNOT00000004686; ENSRNOP00000004686; ENSRNOG00000003370
GeneIDi25611
KEGGirno:25611
UCSCiRGD:3236 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
K03040 mRNA Translation: AAA41768.1
M16933
, M16924, M16925, M16926, M16928, M16929, M16930, M16932 Genomic DNA Translation: AAA41769.1
X01976 mRNA Translation: CAA26007.1
K00001 mRNA Translation: AAA41772.1
M11266 mRNA Translation: AAA41767.1
X01178 mRNA Translation: CAA25618.1
PIRiA00563 OWRT
RefSeqiNP_037210.1, NM_013078.1
UniGeneiRn.2391

3D structure databases

ProteinModelPortaliP00481
SMRiP00481
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004686

PTM databases

iPTMnetiP00481
PhosphoSitePlusiP00481

Proteomic databases

PaxDbiP00481
PRIDEiP00481

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004686; ENSRNOP00000004686; ENSRNOG00000003370
GeneIDi25611
KEGGirno:25611
UCSCiRGD:3236 rat

Organism-specific databases

CTDi5009
RGDi3236 Otc

Phylogenomic databases

eggNOGiKOG1504 Eukaryota
COG0078 LUCA
GeneTreeiENSGT00510000047417
HOGENOMiHOG000022686
HOVERGENiHBG007881
InParanoidiP00481
KOiK00611
OMAiDGNNVCN
OrthoDBiEOG091G0QB1
PhylomeDBiP00481
TreeFamiTF352580

Enzyme and pathway databases

UniPathwayi
UPA00158;UER00271

ReactomeiR-RNO-1268020 Mitochondrial protein import
R-RNO-70635 Urea cycle
SABIO-RKiP00481

Miscellaneous databases

PROiPR:P00481

Gene expression databases

BgeeiENSRNOG00000003370 Expressed in 6 organ(s), highest expression level in liver
GenevisibleiP00481 RN

Family and domain databases

Gene3Di3.40.50.1370, 2 hits
InterProiView protein in InterPro
IPR006132 Asp/Orn_carbamoyltranf_P-bd
IPR006130 Asp/Orn_carbamoylTrfase
IPR036901 Asp/Orn_carbamoylTrfase_sf
IPR006131 Asp_carbamoyltransf_Asp/Orn-bd
IPR002292 Orn/put_carbamltrans
PfamiView protein in Pfam
PF00185 OTCace, 1 hit
PF02729 OTCace_N, 1 hit
PRINTSiPR00100 AOTCASE
PR00102 OTCASE
SUPFAMiSSF53671 SSF53671, 1 hit
TIGRFAMsiTIGR00658 orni_carb_tr, 1 hit
PROSITEiView protein in PROSITE
PS00097 CARBAMOYLTRANSFERASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_RAT
AccessioniPrimary (citable) accession number: P00481
Secondary accession number(s): Q63407
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: November 7, 2018
This is version 163 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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