UniProtKB - P00474 (MTPS_PROST)
Protein
Modification methylase PstI
Gene
pstIM
Organism
Providencia stuartii
Status
Functioni
This methylase recognizes the double-stranded sequence CTGCAG, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the PstI endonuclease.
Catalytic activityi
- a 2'-deoxyadenosine in DNA + S-adenosyl-L-methionine = an N6-methyl-2'-deoxyadenosine in DNA + H+ + S-adenosyl-L-homocysteineEC:2.1.1.72
GO - Molecular functioni
- DNA binding Source: InterPro
- site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC
GO - Biological processi
- DNA restriction-modification system Source: UniProtKB-KW
Keywordsi
Molecular function | Methyltransferase, Transferase |
Biological process | Restriction system |
Ligand | S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Modification methylase PstI (EC:2.1.1.72)Short name: M.PstI Alternative name(s): Adenine-specific methyltransferase PstI |
Gene namesi | Name:pstIM |
Organismi | Providencia stuartii |
Taxonomic identifieri | 588 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacterales › Morganellaceae › Providencia |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000087978 | 1 – 507 | Modification methylase PstIAdd BLAST | 507 |
Interactioni
Subunit structurei
Monomer.
Family & Domainsi
Sequence similaritiesi
Belongs to the N(4)/N(6)-methyltransferase family.Curated
Family and domain databases
InterProi | View protein in InterPro IPR011639, RM_methylase_Eco57I IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF07669, Eco57I, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
i Sequence
Sequence statusi: Complete.
P00474-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEAATQLPI SLNILVDSIR EAANSTLDET LRSKLGQFMS SSAVSELMAN
60 70 80 90 100
LFESYVGEHE ILDAGAGVGS LTAAFVQNAT LNGAKSISST CYEISEVMVY
110 120 130 140 150
NLIQVLDLCK IRAMEFEVNW QQKIIESDFI QASVEQLLIE NYSPKYNKAI
160 170 180 190 200
LNPPYLKIAA KGRERALLQK VGIEASNLYS AFVALAIKQL KSGGELVAIT
210 220 230 240 250
PRSFCNGPYF NDFRKQMLDE CSLNKIHVFN SRKSAFKADN VLQENIIYHL
260 270 280 290 300
TKGETQRKVV TVYSSTCAND INPTIFEVPF DEIVKSNNPD LFIHIVTNEQ
310 320 330 340 350
ERELANKAGG LPCSLSDLGI QVSTGKVVDF RTRENLSMEY ISNSVPLIFP
360 370 380 390 400
QHLQRCSIVW PITKAKKPNA LIVNEATNNL MVPNGIYVLT RRLTAKEEKR
410 420 430 440 450
RIVASIYYPD IANVDTVGFD NKINYFHANG KPLDISLAKG LWVFLNSTLI
460 470 480 490 500
DKYFRQMNGH TQVNATDLRA LRYPTREQLE DIANQVDFGE FEQTKIDEII
NQSLQLM
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02081 Genomic DNA Translation: AAA25672.1 |
PIRi | A00553, XYOFS |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | K02081 Genomic DNA Translation: AAA25672.1 |
PIRi | A00553, XYOFS |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Miscellaneous databases
PROi | PR:P00474 |
Family and domain databases
InterProi | View protein in InterPro IPR011639, RM_methylase_Eco57I IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF07669, Eco57I, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS00092, N6_MTASE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MTPS_PROST | |
Accessioni | P00474Primary (citable) accession number: P00474 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | December 2, 2020 | |
This is version 84 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Documents
- SIMILARITY comments
Index of protein domains and families - Restriction enzymes and methylases
Classification of restriction enzymes and methylases and list of entries