UniProtKB - P00455 (FENR_SPIOL)
Protein
Ferredoxin--NADP reductase, chloroplastic
Gene
PETH
Organism
Spinacia oleracea (Spinach)
Status
Functioni
May play a key role in regulating the relative amounts of cyclic and non-cyclic electron flow to meet the demands of the plant for ATP and reducing power.
Miscellaneous
FNR is probably attached to the membrane by a specific binding protein.
Catalytic activityi
- EC:1.18.1.2
Cofactori
Kineticsi
- KM=35 µM for NADPH1 Publication
: photosynthesis Pathwayi
This protein is involved in the pathway photosynthesis, which is part of Energy metabolism.View all proteins of this organism that are known to be involved in the pathway photosynthesis and in Energy metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 151 | NADPBy similarity | 1 | |
Binding sitei | 171 | NADP | 1 | |
Binding sitei | 175 | FAD2 Publications | 1 | |
Binding sitei | 227 | FAD2 Publications | 1 | |
Binding sitei | 227 | NADP; via amide nitrogenBy similarity | 1 | |
Binding sitei | 367 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 148 – 151 | FAD2 Publications | 4 | |
Nucleotide bindingi | 169 – 171 | FAD2 Publications | 3 | |
Nucleotide bindingi | 186 – 188 | FAD2 Publications | 3 | |
Nucleotide bindingi | 259 – 260 | NADP | 2 | |
Nucleotide bindingi | 289 – 290 | NADP | 2 | |
Nucleotide bindingi | 299 – 301 | NADP | 3 | |
Nucleotide bindingi | 328 – 329 | NADPBy similarity | 2 |
GO - Molecular functioni
- ferredoxin-NADP+ reductase activity Source: UniProtKB-EC
GO - Biological processi
- photosynthesis Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Photosynthesis, Transport |
Ligand | FAD, Flavoprotein, NADP |
Enzyme and pathway databases
BRENDAi | 1.18.1.2, 5812 |
SABIO-RKi | P00455 |
UniPathwayi | UPA00091 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:PETH |
Organismi | Spinacia oleracea (Spinach) |
Taxonomic identifieri | 3562 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › Caryophyllales › Chenopodiaceae › Chenopodioideae › Anserineae › Spinacia |
Subcellular locationi
Chloroplast
- chloroplast stroma
- chloroplast thylakoid membrane Curated; Peripheral membrane protein Curated; Stromal side Curated
Note: In the vicinity of the photosystem I in the non-stacked and fringe portion of the membrane.
Chloroplast
- chloroplast stroma Source: UniProtKB-SubCell
- chloroplast thylakoid membrane Source: UniProtKB-SubCell
Keywords - Cellular componenti
Chloroplast, Membrane, Plastid, ThylakoidPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 367 | E → A: Slightly reduced activity. 1 Publication | 1 | |
Mutagenesisi | 367 | E → D or Q: Reduced activity. 1 Publication | 1 | |
Mutagenesisi | 367 | E → L: Reduces activity by 99%. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 55 | Chloroplast1 PublicationAdd BLAST | 55 | |
ChainiPRO_0000019412 | 56 – 369 | Ferredoxin--NADP reductase, chloroplasticAdd BLAST | 314 |
Proteomic databases
PRIDEi | P00455 |
Interactioni
Binary interactionsi
P00455
With | #Exp. | IntAct |
---|---|---|
PETF [P00221] | 3 | EBI-865079,EBI-864933 |
Protein-protein interaction databases
IntActi | P00455, 2 interactors |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00455 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00455 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 90 – 212 | FAD-binding FR-typePROSITE-ProRule annotationAdd BLAST | 123 |
Sequence similaritiesi
Belongs to the ferredoxin--NADP reductase type 1 family.Curated
Keywords - Domaini
Transit peptideFamily and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR015701, FNR IPR039261, FNR_nucleotide-bd IPR035442, FNR_plant_Cyanobacteria IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF501178, FNR-PetH, 1 hit PIRSF000361, Frd-NADP+_RD, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00455-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTTAVTAAVS FPSTKTTSLS ARSSSVISPD KISYKKVPLY YRNVSATGKM
60 70 80 90 100
GPIRAQIASD VEAPPPAPAK VEKHSKKMEE GITVNKFKPK TPYVGRCLLN
110 120 130 140 150
TKITGDDAPG ETWHMVFSHE GEIPYREGQS VGVIPDGEDK NGKPHKLRLY
160 170 180 190 200
SIASSALGDF GDAKSVSLCV KRLIYTNDAG ETIKGVCSNF LCDLKPGAEV
210 220 230 240 250
KLTGPVGKEM LMPKDPNATI IMLGTGTGIA PFRSFLWKMF FEKHDDYKFN
260 270 280 290 300
GLAWLFLGVP TSSSLLYKEE FEKMKEKAPD NFRLDFAVSR EQTNEKGEKM
310 320 330 340 350
YIQTRMAQYA VELWEMLKKD NTYFYMCGLK GMEKGIDDIM VSLAAAEGID
360
WIEYKRQLKK AEQWNVEVY
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 324 | F → V. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07981 mRNA Translation: CAA30791.1 M86349 mRNA Translation: AAA34029.1 X64351 Genomic DNA Translation: CAA45703.1 |
PIRi | S00438, RDSPXX |
Genome annotation databases
KEGGi | ag:CAA30791 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X07981 mRNA Translation: CAA30791.1 M86349 mRNA Translation: AAA34029.1 X64351 Genomic DNA Translation: CAA45703.1 |
PIRi | S00438, RDSPXX |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BX0 | X-ray | 1.90 | A | 56-369 | [»] | |
1BX1 | X-ray | 1.90 | A | 56-369 | [»] | |
1FNB | X-ray | 1.70 | A | 56-369 | [»] | |
1FNC | X-ray | 2.00 | A | 56-369 | [»] | |
1FND | X-ray | 1.70 | A | 56-369 | [»] | |
1FRN | X-ray | 2.00 | A | 56-369 | [»] | |
1FRQ | X-ray | 1.95 | A | 56-369 | [»] | |
SMRi | P00455 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P00455, 2 interactors |
Proteomic databases
PRIDEi | P00455 |
Genome annotation databases
KEGGi | ag:CAA30791 |
Enzyme and pathway databases
UniPathwayi | UPA00091 |
BRENDAi | 1.18.1.2, 5812 |
SABIO-RKi | P00455 |
Miscellaneous databases
EvolutionaryTracei | P00455 |
Family and domain databases
Gene3Di | 3.40.50.80, 1 hit |
InterProi | View protein in InterPro IPR017927, FAD-bd_FR_type IPR001709, Flavoprot_Pyr_Nucl_cyt_Rdtase IPR015701, FNR IPR039261, FNR_nucleotide-bd IPR035442, FNR_plant_Cyanobacteria IPR001433, OxRdtase_FAD/NAD-bd IPR017938, Riboflavin_synthase-like_b-brl |
Pfami | View protein in Pfam PF00175, NAD_binding_1, 1 hit |
PIRSFi | PIRSF501178, FNR-PetH, 1 hit PIRSF000361, Frd-NADP+_RD, 1 hit |
PRINTSi | PR00371, FPNCR |
SUPFAMi | SSF52343, SSF52343, 1 hit SSF63380, SSF63380, 1 hit |
PROSITEi | View protein in PROSITE PS51384, FAD_FR, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FENR_SPIOL | |
Accessioni | P00455Primary (citable) accession number: P00455 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | November 1, 1988 | |
Last modified: | December 2, 2020 | |
This is version 136 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencingDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families