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Protein

Cytochrome c oxidase subunit 1

Gene

MT-CO1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Cytochrome c oxidase is the component of the respiratory chain that catalyzes the reduction of oxygen to water. Subunits 1-3 form the functional core of the enzyme complex. CO I is the catalytic subunit of the enzyme. Electrons originating in cytochrome c are transferred via the copper A center of subunit 2 and heme A of subunit 1 to the bimetallic center formed by heme A3 and copper B.

Catalytic activityi

4 ferrocytochrome c + O2 + 4 H+ = 4 ferricytochrome c + 2 H2O.

Pathwayi: oxidative phosphorylation

This protein is involved in the pathway oxidative phosphorylation, which is part of Energy metabolism.
View all proteins of this organism that are known to be involved in the pathway oxidative phosphorylation and in Energy metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi61Iron (heme A axial ligand)1 Publication1
Metal bindingi240Copper B1 Publication1
Binding sitei244OxygenCurated1
Metal bindingi290Copper B1 Publication1
Metal bindingi291Copper B1 Publication1
Metal bindingi368Magnesium; shared with chain II1 Publication1
Metal bindingi376Iron (heme A3 axial ligand)1 Publication1
Metal bindingi378Iron (heme A axial ligand)1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Respiratory chain, Transport
LigandCopper, Heme, Iron, Metal-binding

Enzyme and pathway databases

BRENDAi1.9.3.1 908
SABIO-RKiP00396
UniPathwayi
UPA00705

Protein family/group databases

TCDBi3.D.4.7.1 the proton-translocating cytochrome oxidase (cox) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c oxidase subunit 1 (EC:1.9.3.1)
Alternative name(s):
Cytochrome c oxidase polypeptide I
Gene namesi
Name:MT-CO1
Synonyms:COI, COXI, MTCO1
Encoded oniMitochondrion
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Mitochondrion

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 11Mitochondrial matrix1 PublicationAdd BLAST11
Transmembranei12 – 40Helical; Name=IAdd BLAST29
Topological domaini41 – 50Mitochondrial intermembrane1 Publication10
Transmembranei51 – 86Helical; Name=IIAdd BLAST36
Topological domaini87 – 94Mitochondrial matrix1 Publication8
Transmembranei95 – 117Helical; Name=IIIAdd BLAST23
Topological domaini118 – 140Mitochondrial intermembrane1 PublicationAdd BLAST23
Transmembranei141 – 170Helical; Name=IVAdd BLAST30
Topological domaini171 – 182Mitochondrial matrix1 PublicationAdd BLAST12
Transmembranei183 – 212Helical; Name=VAdd BLAST30
Topological domaini213 – 227Mitochondrial intermembrane1 PublicationAdd BLAST15
Transmembranei228 – 261Helical; Name=VIAdd BLAST34
Topological domaini262 – 269Mitochondrial matrix1 Publication8
Transmembranei270 – 286Helical; Name=VIIAdd BLAST17
Topological domaini287 – 298Mitochondrial intermembrane1 PublicationAdd BLAST12
Transmembranei299 – 327Helical; Name=VIIIAdd BLAST29
Topological domaini328 – 335Mitochondrial matrix8
Transmembranei336 – 357Helical; Name=IXAdd BLAST22
Topological domaini358 – 370Mitochondrial intermembraneAdd BLAST13
Transmembranei371 – 400Helical; Name=XAdd BLAST30
Topological domaini401 – 406Mitochondrial matrix1 Publication6
Transmembranei407 – 433Helical; Name=XIAdd BLAST27
Topological domaini434 – 446Mitochondrial intermembraneAdd BLAST13
Transmembranei447 – 478Helical; Name=XIIAdd BLAST32
Topological domaini479 – 514Mitochondrial matrix1 PublicationAdd BLAST36

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001832941 – 514Cytochrome c oxidase subunit 1Add BLAST514

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-formylmethionine1 Publication1
Cross-linki240 ↔ 2441'-histidyl-3'-tyrosine (His-Tyr)1 Publication

Post-translational modificationi

His-240 and Tyr-244 are involved in the formation of a copper-coordinated covalent cross-link at the active site of the catalytic subunit I.

Keywords - PTMi

Formylation

Proteomic databases

PaxDbiP00396
PRIDEiP00396

PTM databases

iPTMnetiP00396

Interactioni

Subunit structurei

As a newly synthesized protein, rapidly incorporates into a multi-subunit assembly intermediate in the inner membrane, called MITRAC (mitochondrial translation regulation assembly intermediate of cytochrome c oxidase) complex, whose core components are COA3/MITRAC12 and COX14. Within the MITRAC complex, interacts with COA3 and with SMIM20/MITRAC7; the interaction with SMIM20 stabilizes the newly synthesized MT-CO1 and prevents its premature turnover. Interacts with TMEM177 in a COX20-dependent manner.By similarity

Protein-protein interaction databases

CORUMiP00396
DIPiDIP-60937N
IntActiP00396, 13 interactors
MINTiP00396
STRINGi9913.ENSBTAP00000053147

Structurei

Secondary structure

1514
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00396
SMRiP00396
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00396

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4769 Eukaryota
COG0843 LUCA
HOGENOMiHOG000085274
HOVERGENiHBG003841
InParanoidiP00396

Family and domain databases

CDDicd01663 Cyt_c_Oxidase_I, 1 hit
Gene3Di1.20.210.10, 1 hit
InterProiView protein in InterPro
IPR023616 Cyt_c_oxase-like_su1_dom
IPR036927 Cyt_c_oxase-like_su1_sf
IPR000883 Cyt_C_Oxase_1
IPR023615 Cyt_c_Oxase_su1_BS
IPR033944 Cyt_c_oxase_su1_dom
PANTHERiPTHR10422 PTHR10422, 1 hit
PfamiView protein in Pfam
PF00115 COX1, 1 hit
PRINTSiPR01165 CYCOXIDASEI
SUPFAMiSSF81442 SSF81442, 1 hit
PROSITEiView protein in PROSITE
PS50855 COX1, 1 hit
PS00077 COX1_CUB, 1 hit

Sequencei

Sequence statusi: Complete.

P00396-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MFINRWLFST NHKDIGTLYL LFGAWAGMVG TALSLLIRAE LGQPGTLLGD
60 70 80 90 100
DQIYNVVVTA HAFVMIFFMV MPIMIGGFGN WLVPLMIGAP DMAFPRMNNM
110 120 130 140 150
SFWLLPPSFL LLLASSMVEA GAGTGWTVYP PLAGNLAHAG ASVDLTIFSL
160 170 180 190 200
HLAGVSSILG AINFITTIIN MKPPAMSQYQ TPLFVWSVMI TAVLLLLSLP
210 220 230 240 250
VLAAGITMLL TDRNLNTTFF DPAGGGDPIL YQHLFWFFGH PEVYILILPG
260 270 280 290 300
FGMISHIVTY YSGKKEPFGY MGMVWAMMSI GFLGFIVWAH HMFTVGMDVD
310 320 330 340 350
TRAYFTSATM IIAIPTGVKV FSWLATLHGG NIKWSPAMMW ALGFIFLFTV
360 370 380 390 400
GGLTGIVLAN SSLDIVLHDT YYVVAHFHYV LSMGAVFAIM GGFVHWFPLF
410 420 430 440 450
SGYTLNDTWA KIHFAIMFVG VNMTFFPQHF LGLSGMPRRY SDYPDAYTMW
460 470 480 490 500
NTISSMGSFI SLTAVMLMVF IIWEAFASKR EVLTVDLTTT NLEWLNGCPP
510
PYHTFEEPTY VNLK
Length:514
Mass (Da):57,032
Last modified:July 21, 1986 - v1
Checksum:i0D7C807D7FA3996C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA Translation: CAA23999.1
AF490528 Genomic DNA Translation: AAM08330.1
AF490529 Genomic DNA Translation: AAM08343.1
AF493541 Genomic DNA Translation: AAM12791.1
AF493542 Genomic DNA Translation: AAM12804.1
PIRiA00464 ODBO1

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA Translation: CAA23999.1
AF490528 Genomic DNA Translation: AAM08330.1
AF490529 Genomic DNA Translation: AAM08343.1
AF493541 Genomic DNA Translation: AAM12791.1
AF493542 Genomic DNA Translation: AAM12804.1
PIRiA00464 ODBO1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1OCCX-ray2.80A/N1-514[»]
1OCOX-ray2.80A/N1-514[»]
1OCRX-ray2.35A/N1-514[»]
1OCZX-ray2.90A/N1-514[»]
1V54X-ray1.80A/N1-514[»]
1V55X-ray1.90A/N1-514[»]
2DYRX-ray1.80A/N1-514[»]
2DYSX-ray2.20A/N1-514[»]
2EIJX-ray1.90A/N1-514[»]
2EIKX-ray2.10A/N1-514[»]
2EILX-ray2.10A/N1-514[»]
2EIMX-ray2.60A/N1-514[»]
2EINX-ray2.70A/N1-514[»]
2OCCX-ray2.30A/N1-514[»]
2Y69X-ray1.95A/N1-514[»]
2YBBelectron microscopy19.00L1-514[»]
2ZXWX-ray2.50A/N1-514[»]
3ABKX-ray2.00A/N1-514[»]
3ABLX-ray2.10A/N1-514[»]
3ABMX-ray1.95A/N1-514[»]
3AG1X-ray2.20A/N1-514[»]
3AG2X-ray1.80A/N1-514[»]
3AG3X-ray1.80A/N1-514[»]
3AG4X-ray2.05A/N1-514[»]
3ASNX-ray3.00A/N1-514[»]
3ASOX-ray2.30A/N1-514[»]
3WG7X-ray1.90A/N1-514[»]
3X2QX-ray2.00A/N1-514[»]
5B1AX-ray1.50A/N1-514[»]
5B1BX-ray1.60A/N1-514[»]
5B3SX-ray1.68A/N2-514[»]
5GPNelectron microscopy5.40y1-514[»]
5IY5X-ray2.00A/N1-514[»]
5LUFelectron microscopy9.10x1-514[»]
5W97X-ray2.30A/a1-514[»]
5WAUX-ray1.95A/a1-514[»]
5X19X-ray2.20A/N1-514[»]
5X1BX-ray2.40A/N1-514[»]
5X1FX-ray2.20A/N1-514[»]
5XDQX-ray1.77A/N1-514[»]
5XDXX-ray1.99A/N1-514[»]
5XTHelectron microscopy3.90x1-514[»]
5XTIelectron microscopy17.40Bx/x1-514[»]
5Z84X-ray1.85A/N1-514[»]
5Z85X-ray1.85A/N1-514[»]
5Z86X-ray1.85A/N1-514[»]
5ZCOX-ray1.90A/N1-514[»]
5ZCPX-ray1.65A/N1-514[»]
5ZCQX-ray1.65A/N1-514[»]
ProteinModelPortaliP00396
SMRiP00396
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiP00396
DIPiDIP-60937N
IntActiP00396, 13 interactors
MINTiP00396
STRINGi9913.ENSBTAP00000053147

Protein family/group databases

TCDBi3.D.4.7.1 the proton-translocating cytochrome oxidase (cox) superfamily

PTM databases

iPTMnetiP00396

Proteomic databases

PaxDbiP00396
PRIDEiP00396

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG4769 Eukaryota
COG0843 LUCA
HOGENOMiHOG000085274
HOVERGENiHBG003841
InParanoidiP00396

Enzyme and pathway databases

UniPathwayi
UPA00705

BRENDAi1.9.3.1 908
SABIO-RKiP00396

Miscellaneous databases

EvolutionaryTraceiP00396

Family and domain databases

CDDicd01663 Cyt_c_Oxidase_I, 1 hit
Gene3Di1.20.210.10, 1 hit
InterProiView protein in InterPro
IPR023616 Cyt_c_oxase-like_su1_dom
IPR036927 Cyt_c_oxase-like_su1_sf
IPR000883 Cyt_C_Oxase_1
IPR023615 Cyt_c_Oxase_su1_BS
IPR033944 Cyt_c_oxase_su1_dom
PANTHERiPTHR10422 PTHR10422, 1 hit
PfamiView protein in Pfam
PF00115 COX1, 1 hit
PRINTSiPR01165 CYCOXIDASEI
SUPFAMiSSF81442 SSF81442, 1 hit
PROSITEiView protein in PROSITE
PS50855 COX1, 1 hit
PS00077 COX1_CUB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCOX1_BOVIN
AccessioniPrimary (citable) accession number: P00396
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: October 10, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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