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Protein

NADPH--cytochrome P450 reductase

Gene

Por

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.UniRule annotation

Catalytic activityi

NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein.UniRule annotation

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei208FMNUniRule annotation3 Publications1
Binding sitei298NADPUniRule annotation4 Publications1
Binding sitei424FADUniRule annotation4 Publications1
Binding sitei478FADUniRule annotation5 Publications1
Binding sitei535NADPUniRule annotation5 Publications1
Binding sitei639NADPUniRule annotation4 Publications1
Binding sitei677FADUniRule annotation5 Publications1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi86 – 91FMNUniRule annotation5 Publications6
Nucleotide bindingi138 – 141FMNUniRule annotation5 Publications4
Nucleotide bindingi173 – 182FMNUniRule annotation5 Publications10
Nucleotide bindingi454 – 457FADUniRule annotation5 Publications4
Nucleotide bindingi472 – 474FADUniRule annotation4 Publications3
Nucleotide bindingi488 – 491FADUniRule annotation5 Publications4
Nucleotide bindingi596 – 597NADPUniRule annotation5 Publications2
Nucleotide bindingi602 – 606NADPUniRule annotation5 Publications5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandFAD, Flavoprotein, FMN, NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14290
BRENDAi1.6.2.4 5301
ReactomeiR-RNO-211897 Cytochrome P450 - arranged by substrate type
SABIO-RKiP00388

Names & Taxonomyi

Protein namesi
Recommended name:
NADPH--cytochrome P450 reductaseUniRule annotation (EC:1.6.2.4UniRule annotation)
Short name:
CPRUniRule annotation
Short name:
P450RUniRule annotation
Gene namesi
Name:PorUniRule annotation
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi68335 Por

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 22LumenalUniRule annotationAdd BLAST21
Transmembranei23 – 43HelicalUniRule annotationAdd BLAST21
Topological domaini44 – 678CytoplasmicUniRule annotationAdd BLAST635

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2817

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001676002 – 678NADPH--cytochrome P450 reductaseAdd BLAST677

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP00388
PRIDEiP00388

PTM databases

iPTMnetiP00388
PhosphoSitePlusiP00388

Expressioni

Gene expression databases

BgeeiENSRNOG00000001442 Expressed in 10 organ(s), highest expression level in liver
GenevisibleiP00388 RN

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi248088, 1 interactor
STRINGi10116.ENSRNOP00000001961

Structurei

Secondary structure

1678
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00388
SMRiP00388
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00388

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini80 – 224Flavodoxin-likeUniRule annotationAdd BLAST145
Domaini279 – 521FAD-binding FR-typeUniRule annotationAdd BLAST243

Sequence similaritiesi

Belongs to the NADPH--cytochrome P450 reductase family.UniRule annotation
In the N-terminal section; belongs to the flavodoxin family.UniRule annotation
In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1158 Eukaryota
COG0369 LUCA
GeneTreeiENSGT00840000129757
HOGENOMiHOG000282027
HOVERGENiHBG000432
InParanoidiP00388
KOiK00327
OMAiTKMDVAF
OrthoDBiEOG091G02VT
PhylomeDBiP00388
TreeFamiTF105719

Family and domain databases

Gene3Di1.20.990.10, 4 hits
3.40.50.360, 1 hit
3.40.50.80, 1 hit
HAMAPiMF_03212 NCPR, 1 hit
InterProiView protein in InterPro
IPR003097 FAD-binding_1
IPR017927 Fd_Rdtase_FAD-bd
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001709 Flavoprot_Pyr_Nucl_cyt_Rdtase
IPR029039 Flavoprotein-like_sf
IPR039261 FNR_nucleotide-bd
IPR023173 NADPH_Cyt_P450_Rdtase_alpha
IPR001433 OxRdtase_FAD/NAD-bd
IPR023208 P450R
IPR017938 Riboflavin_synthase-like_b-brl
PfamiView protein in Pfam
PF00667 FAD_binding_1, 1 hit
PF00258 Flavodoxin_1, 1 hit
PF00175 NAD_binding_1, 1 hit
PIRSFiPIRSF000208 P450R, 1 hit
PRINTSiPR00369 FLAVODOXIN
PR00371 FPNCR
SUPFAMiSSF52218 SSF52218, 1 hit
SSF52343 SSF52343, 1 hit
SSF63380 SSF63380, 1 hit
PROSITEiView protein in PROSITE
PS51384 FAD_FR, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00388-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGDSHEDTSA TMPEAVAEEV SLFSTTDMVL FSLIVGVLTY WFIFRKKKEE
60 70 80 90 100
IPEFSKIQTT APPVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK
110 120 130 140 150
DAHRYGMRGM SADPEEYDLA DLSSLPEIDK SLVVFCMATY GEGDPTDNAQ
160 170 180 190 200
DFYDWLQETD VDLTGVKFAV FGLGNKTYEH FNAMGKYVDQ RLEQLGAQRI
210 220 230 240 250
FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES SIRQYELVVH
260 270 280 290 300
EDMDVAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTANR KLNQGTERHL
310 320 330 340 350
MHLELDISDS KIRYESGDHV AVYPANDSAL VNQIGEILGA DLDVIMSLNN
360 370 380 390 400
LDEESNKKHP FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE
410 420 430 440 450
HLHKMASSSG EGKELYLSWV VEARRHILAI LQDYPSLRPP IDHLCELLPR
460 470 480 490 500
LQARYYSIAS SSKVHPNSVH ICAVAVEYEA KSGRVNKGVA TSWLRAKEPA
510 520 530 540 550
GENGGRALVP MFVRKSQFRL PFKSTTPVIM VGPGTGIAPF MGFIQERAWL
560 570 580 590 600
REQGKEVGET LLYYGCRRSD EDYLYREELA RFHKDGALTQ LNVAFSREQA
610 620 630 640 650
HKVYVQHLLK RDREHLWKLI HEGGAHIYVC GDARNMAKDV QNTFYDIVAE
660 670
FGPMEHTQAV DYVKKLMTKG RYSLDVWS
Length:678
Mass (Da):76,963
Last modified:January 23, 2007 - v3
Checksum:iAF3087E4D7A352D6
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti255V → A in AAA41683 (PubMed:2125483).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M10068 mRNA Translation: AAA41064.1
M12516 mRNA Translation: AAA41067.1
M58937
, M58932, M58933, M58934, M58935, M58936 Genomic DNA Translation: AAA41683.1
PIRiA36073 RDRTO4
RefSeqiNP_113764.1, NM_031576.1
UniGeneiRn.11359

Genome annotation databases

EnsembliENSRNOT00000001961; ENSRNOP00000001961; ENSRNOG00000001442
GeneIDi29441
KEGGirno:29441
UCSCiRGD:68335 rat

Similar proteinsi

Entry informationi

Entry nameiNCPR_RAT
AccessioniPrimary (citable) accession number: P00388
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 179 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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