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Protein

Glyceraldehyde-3-phosphate dehydrogenase 1

Gene

TDH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

There are three genes for G3PDH in yeast.
Present with 120000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3), Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1), Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase 1 (GPM1), Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 3 (GPM3)
  4. Enolase-related protein 3 (ERR3), Enolase-related protein 1 (ERR1), Enolase-related protein 2 (ERR2), Enolase 2 (ENO2), Enolase 1 (ENO1)
  5. Pyruvate kinase 1 (CDC19), Pyruvate kinase 2 (PYK2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NADBy similarity1
Binding sitei78NAD; via carbonyl oxygenBy similarity1
Active sitei150NucleophilePROSITE-ProRule annotation1
Sitei177Activates thiol group during catalysisBy similarity1
Binding sitei180Glyceraldehyde 3-phosphateBy similarity1
Binding sitei232Glyceraldehyde 3-phosphateBy similarity1
Binding sitei314NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADBy similarity2

GO - Molecular functioni

GO - Biological processi

  • gluconeogenesis Source: SGD
  • glycolytic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD

Enzyme and pathway databases

BioCyciYEAST:YJL052W-MONOMER
SABIO-RKiP00360
UniPathwayi
UPA00109;UER00184

Protein family/group databases

MoonDBiP00360 Curated

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 1 (EC:1.2.1.12)
Short name:
GAPDH 1
Gene namesi
Name:TDH1
Synonyms:GPD1, SSS2
Ordered Locus Names:YJL052W
ORF Names:J1154
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

SGDiS000003588 TDH1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001455891 – 332Glyceraldehyde-3-phosphate dehydrogenase 1Add BLAST332

Proteomic databases

MaxQBiP00360
PaxDbiP00360
PRIDEiP00360
TopDownProteomicsiP00360

2D gel databases

SWISS-2DPAGEiP00360

PTM databases

iPTMnetiP00360

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi33703, 76 interactors
DIPiDIP-4304N
IntActiP00360, 67 interactors
MINTiP00360
STRINGi4932.YJL052W

Structurei

3D structure databases

ProteinModelPortaliP00360
SMRiP00360
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni149 – 151Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni209 – 210Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00760000119172
HOGENOMiHOG000071678
InParanoidiP00360
KOiK00134
OMAiFTLENMV
OrthoDBiEOG092C2MZH

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

P00360-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MIRIAINGFG RIGRLVLRLA LQRKDIEVVA VNDPFISNDY AAYMVKYDST
60 70 80 90 100
HGRYKGTVSH DDKHIIIDGV KIATYQERDP ANLPWGSLKI DVAVDSTGVF
110 120 130 140 150
KELDTAQKHI DAGAKKVVIT APSSSAPMFV VGVNHTKYTP DKKIVSNASC
160 170 180 190 200
TTNCLAPLAK VINDAFGIEE GLMTTVHSMT ATQKTVDGPS HKDWRGGRTA
210 220 230 240 250
SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLEKE
260 270 280 290 300
ATYDQIKKAV KAAAEGPMKG VLGYTEDAVV SSDFLGDTHA SIFDASAGIQ
310 320 330
LSPKFVKLIS WYDNEYGYSA RVVDLIEYVA KA
Length:332
Mass (Da):35,750
Last modified:January 23, 2007 - v3
Checksum:i4C116C86EAF3DB70
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti248E → A in CAA24609 (PubMed:6833300).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01302 Genomic DNA Translation: CAA24609.1
Z49327 Genomic DNA Translation: CAA89343.1
AY693001 Genomic DNA Translation: AAT93020.1
BK006943 Genomic DNA Translation: DAA08747.1
PIRiS56824 DEBYG3
RefSeqiNP_012483.3, NM_001181485.3

Genome annotation databases

EnsemblFungiiYJL052W; YJL052W; YJL052W
GeneIDi853395
KEGGisce:YJL052W

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01302 Genomic DNA Translation: CAA24609.1
Z49327 Genomic DNA Translation: CAA89343.1
AY693001 Genomic DNA Translation: AAT93020.1
BK006943 Genomic DNA Translation: DAA08747.1
PIRiS56824 DEBYG3
RefSeqiNP_012483.3, NM_001181485.3

3D structure databases

ProteinModelPortaliP00360
SMRiP00360
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33703, 76 interactors
DIPiDIP-4304N
IntActiP00360, 67 interactors
MINTiP00360
STRINGi4932.YJL052W

Protein family/group databases

MoonDBiP00360 Curated

PTM databases

iPTMnetiP00360

2D gel databases

SWISS-2DPAGEiP00360

Proteomic databases

MaxQBiP00360
PaxDbiP00360
PRIDEiP00360
TopDownProteomicsiP00360

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJL052W; YJL052W; YJL052W
GeneIDi853395
KEGGisce:YJL052W

Organism-specific databases

SGDiS000003588 TDH1

Phylogenomic databases

GeneTreeiENSGT00760000119172
HOGENOMiHOG000071678
InParanoidiP00360
KOiK00134
OMAiFTLENMV
OrthoDBiEOG092C2MZH

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00184

BioCyciYEAST:YJL052W-MONOMER
SABIO-RKiP00360

Miscellaneous databases

PROiPR:P00360

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiG3P1_YEAST
AccessioniPrimary (citable) accession number: P00360
Secondary accession number(s): D6VWD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 194 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names
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Main funding by: National Institutes of Health

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