UniProtKB - P00360 (G3P1_YEAST)
Protein
Glyceraldehyde-3-phosphate dehydrogenase 1
Gene
TDH1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Miscellaneous
There are three genes for G3PDH in yeast.
Present with 120000 molecules/cell in log phase SD medium.1 Publication
Catalytic activityi
- D-glyceraldehyde 3-phosphate + NAD+ + phosphate = (2R)-3-phospho-glyceroyl phosphate + H+ + NADHPROSITE-ProRule annotationEC:1.2.1.12PROSITE-ProRule annotation
: glycolysis Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Proteins known to be involved in the 5 steps of the subpathway in this organism are:
- Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2), Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3), Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1)
- Phosphoglycerate kinase (PGK1)
- Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 1 (GPM1), Phosphoglycerate mutase 3 (GPM3)
- Enolase 2 (ENO2), Enolase-related protein 2 (ERR2), Enolase-related protein 1 (ERR1), Enolase-related protein 3 (ERR3), Enolase 1 (ENO1)
- Pyruvate kinase 1 (CDC19), Pyruvate kinase 2 (PYK2)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 33 | NADBy similarity | 1 | |
Binding sitei | 78 | NAD; via carbonyl oxygenBy similarity | 1 | |
Active sitei | 150 | NucleophilePROSITE-ProRule annotation | 1 | |
Sitei | 177 | Activates thiol group during catalysisBy similarity | 1 | |
Binding sitei | 180 | Glyceraldehyde 3-phosphateBy similarity | 1 | |
Binding sitei | 232 | Glyceraldehyde 3-phosphateBy similarity | 1 | |
Binding sitei | 314 | NADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 11 – 12 | NADBy similarity | 2 |
GO - Molecular functioni
- glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity Source: SGD
- melatonin binding Source: SGD
- NAD binding Source: InterPro
- NADP binding Source: InterPro
GO - Biological processi
- gluconeogenesis Source: SGD
- glycolytic process Source: SGD
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Glycolysis |
Ligand | NAD |
Enzyme and pathway databases
Reactomei | R-SCE-70171, Glycolysis R-SCE-70263, Gluconeogenesis |
SABIO-RKi | P00360 |
UniPathwayi | UPA00109;UER00184 |
Protein family/group databases
MoonDBi | P00360, Curated |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:TDH1 Synonyms:GPD1, SSS2 Ordered Locus Names:YJL052W ORF Names:J1154 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
EuPathDBi | FungiDB:YJL052W |
SGDi | S000003588, TDH1 |
Subcellular locationi
Other locations
- Cytoplasm 1 Publication
Cell Wall
- fungal-type cell wall Source: SGD
Cytosol
- cytosol Source: SGD
Mitochondrion
- mitochondrion Source: SGD
Plasma Membrane
- plasma membrane Source: SGD
Other locations
- lipid droplet Source: SGD
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000145589 | 1 – 332 | Glyceraldehyde-3-phosphate dehydrogenase 1Add BLAST | 332 |
Proteomic databases
MaxQBi | P00360 |
PaxDbi | P00360 |
PRIDEi | P00360 |
TopDownProteomicsi | P00360 |
2D gel databases
SWISS-2DPAGEi | P00360 |
PTM databases
iPTMneti | P00360 |
Interactioni
Subunit structurei
Homotetramer.
Protein-protein interaction databases
BioGRIDi | 33703, 79 interactors |
DIPi | DIP-4304N |
IntActi | P00360, 67 interactors |
MINTi | P00360 |
STRINGi | 4932.YJL052W |
Miscellaneous databases
RNActi | P00360, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 149 – 151 | Glyceraldehyde 3-phosphate bindingBy similarity | 3 | |
Regioni | 209 – 210 | Glyceraldehyde 3-phosphate bindingBy similarity | 2 |
Sequence similaritiesi
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG0657, Eukaryota |
GeneTreei | ENSGT00940000153298 |
HOGENOMi | CLU_030140_0_3_1 |
InParanoidi | P00360 |
OMAi | HETYKGE |
Family and domain databases
InterProi | View protein in InterPro IPR020831, GlycerAld/Erythrose_P_DH IPR020830, GlycerAld_3-P_DH_AS IPR020829, GlycerAld_3-P_DH_cat IPR020828, GlycerAld_3-P_DH_NAD(P)-bd IPR006424, Glyceraldehyde-3-P_DH_1 IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR10836, PTHR10836, 1 hit |
Pfami | View protein in Pfam PF02800, Gp_dh_C, 1 hit PF00044, Gp_dh_N, 1 hit |
PIRSFi | PIRSF000149, GAP_DH, 1 hit |
PRINTSi | PR00078, G3PDHDRGNASE |
SMARTi | View protein in SMART SM00846, Gp_dh_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01534, GAPDH-I, 1 hit |
PROSITEi | View protein in PROSITE PS00071, GAPDH, 1 hit |
i Sequence
Sequence statusi: Complete.
P00360-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIRIAINGFG RIGRLVLRLA LQRKDIEVVA VNDPFISNDY AAYMVKYDST
60 70 80 90 100
HGRYKGTVSH DDKHIIIDGV KIATYQERDP ANLPWGSLKI DVAVDSTGVF
110 120 130 140 150
KELDTAQKHI DAGAKKVVIT APSSSAPMFV VGVNHTKYTP DKKIVSNASC
160 170 180 190 200
TTNCLAPLAK VINDAFGIEE GLMTTVHSMT ATQKTVDGPS HKDWRGGRTA
210 220 230 240 250
SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLEKE
260 270 280 290 300
ATYDQIKKAV KAAAEGPMKG VLGYTEDAVV SSDFLGDTHA SIFDASAGIQ
310 320 330
LSPKFVKLIS WYDNEYGYSA RVVDLIEYVA KA
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 248 | E → A in CAA24609 (PubMed:6833300).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01302 Genomic DNA Translation: CAA24609.1 Z49327 Genomic DNA Translation: CAA89343.1 AY693001 Genomic DNA Translation: AAT93020.1 BK006943 Genomic DNA Translation: DAA08747.1 |
PIRi | S56824, DEBYG3 |
RefSeqi | NP_012483.3, NM_001181485.3 |
Genome annotation databases
EnsemblFungii | YJL052W_mRNA; YJL052W; YJL052W |
GeneIDi | 853395 |
KEGGi | sce:YJL052W |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V01302 Genomic DNA Translation: CAA24609.1 Z49327 Genomic DNA Translation: CAA89343.1 AY693001 Genomic DNA Translation: AAT93020.1 BK006943 Genomic DNA Translation: DAA08747.1 |
PIRi | S56824, DEBYG3 |
RefSeqi | NP_012483.3, NM_001181485.3 |
3D structure databases
SMRi | P00360 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 33703, 79 interactors |
DIPi | DIP-4304N |
IntActi | P00360, 67 interactors |
MINTi | P00360 |
STRINGi | 4932.YJL052W |
Protein family/group databases
MoonDBi | P00360, Curated |
PTM databases
iPTMneti | P00360 |
2D gel databases
SWISS-2DPAGEi | P00360 |
Proteomic databases
MaxQBi | P00360 |
PaxDbi | P00360 |
PRIDEi | P00360 |
TopDownProteomicsi | P00360 |
Genome annotation databases
EnsemblFungii | YJL052W_mRNA; YJL052W; YJL052W |
GeneIDi | 853395 |
KEGGi | sce:YJL052W |
Organism-specific databases
EuPathDBi | FungiDB:YJL052W |
SGDi | S000003588, TDH1 |
Phylogenomic databases
eggNOGi | KOG0657, Eukaryota |
GeneTreei | ENSGT00940000153298 |
HOGENOMi | CLU_030140_0_3_1 |
InParanoidi | P00360 |
OMAi | HETYKGE |
Enzyme and pathway databases
UniPathwayi | UPA00109;UER00184 |
Reactomei | R-SCE-70171, Glycolysis R-SCE-70263, Gluconeogenesis |
SABIO-RKi | P00360 |
Miscellaneous databases
ChiTaRSi | GPD1, yeast |
PROi | PR:P00360 |
RNActi | P00360, protein |
Family and domain databases
InterProi | View protein in InterPro IPR020831, GlycerAld/Erythrose_P_DH IPR020830, GlycerAld_3-P_DH_AS IPR020829, GlycerAld_3-P_DH_cat IPR020828, GlycerAld_3-P_DH_NAD(P)-bd IPR006424, Glyceraldehyde-3-P_DH_1 IPR036291, NAD(P)-bd_dom_sf |
PANTHERi | PTHR10836, PTHR10836, 1 hit |
Pfami | View protein in Pfam PF02800, Gp_dh_C, 1 hit PF00044, Gp_dh_N, 1 hit |
PIRSFi | PIRSF000149, GAP_DH, 1 hit |
PRINTSi | PR00078, G3PDHDRGNASE |
SMARTi | View protein in SMART SM00846, Gp_dh_N, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
TIGRFAMsi | TIGR01534, GAPDH-I, 1 hit |
PROSITEi | View protein in PROSITE PS00071, GAPDH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | G3P1_YEAST | |
Accessioni | P00360Primary (citable) accession number: P00360 Secondary accession number(s): D6VWD1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 208 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families - Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names