TDH1

Functionⁱ

Miscellaneous

There are three genes for G3PDH in yeast.

Present with 120000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityⁱ

D-glyceraldehyde 3-phosphate + phosphate + NAD⁺ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayⁱ: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3), Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1), Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2)
Phosphoglycerate kinase (PGK1)
Phosphoglycerate mutase 1 (GPM1), Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 3 (GPM3)
Enolase-related protein 3 (ERR3), Enolase-related protein 1 (ERR1), Enolase-related protein 2 (ERR2), Enolase 2 (ENO2), Enolase 1 (ENO1)
Pyruvate kinase 1 (CDC19), Pyruvate kinase 2 (PYK2)

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	33	NADBy similarity	1
Binding siteⁱ	78	NAD; via carbonyl oxygenBy similarity	1
Active siteⁱ	150	NucleophilePROSITE-ProRule annotation	1
Siteⁱ	177	Activates thiol group during catalysisBy similarity	1
Binding siteⁱ	180	Glyceraldehyde 3-phosphateBy similarity	1
Binding siteⁱ	232	Glyceraldehyde 3-phosphateBy similarity	1
Binding siteⁱ	314	NADBy similarity	1

Regions

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Nucleotide bindingⁱ	11 – 12	NADBy similarity		2

GO - Molecular functionⁱ

glyceraldehyde-3-phosphate dehydrogenase (NAD+) (phosphorylating) activity
Source: SGD
Inferred from direct assayⁱ
- 3905788
NAD binding Source: InterPro
NADP binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

gluconeogenesis
Source: SGD
Inferred from expression patternⁱ
- 3905788
glycolytic process
Source: SGD
Inferred from expression patternⁱ
- 3905788

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Glycolysis
Ligand	NAD

Enzyme and pathway databases

BioCycⁱ	YEAST:YJL052W-MONOMER
SABIO-RKⁱ	P00360
UniPathwayⁱ	UPA00109;UER00184

Protein family/group databases

MoonDBⁱ	P00360 Curated

MoonDBⁱ

P00360 Curated

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Glyceraldehyde-3-phosphate dehydrogenase 1 (EC:1.2.1.12) Short name: GAPDH 1
Gene namesⁱ	Name:TDH1 Synonyms:GPD1, SSS2 Ordered Locus Names:YJL052W ORF Names:J1154
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Chromosome X

Organism-specific databases

Saccharomyces Genome Database More...SGDⁱ	S000003588 TDH1

SGDⁱ

S000003588 TDH1

Keywords - Cellular componentⁱ

Cytoplasm

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000145589	1 – 332	Glyceraldehyde-3-phosphate dehydrogenase 1Add BLAST		332

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	P00360
PaxDbⁱ	P00360
PRIDEⁱ	P00360
TopDownProteomicsⁱ	P00360

2D gel databases

SWISS-2DPAGEⁱ	P00360

SWISS-2DPAGEⁱ

P00360

PTM databases

iPTMnetⁱ	P00360

iPTMnetⁱ

P00360

Interactionⁱ

Subunit structureⁱ

Homotetramer.

Protein-protein interaction databases

BioGridⁱ	33703, 76 interactors
Database of interacting proteins More...DIPⁱ	DIP-4304N
IntActⁱ	P00360, 67 interactors
Molecular INTeraction database More...MINTⁱ	P00360
STRINGⁱ	4932.YJL052W

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	P00360
SMRⁱ	P00360
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	149 – 151	Glyceraldehyde 3-phosphate bindingBy similarity		3
Regionⁱ	209 – 210	Glyceraldehyde 3-phosphate bindingBy similarity		2

Sequence similaritiesⁱ

Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.Curated

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00760000119172
HOGENOMⁱ	HOG000071678
InParanoidⁱ	P00360
KEGG Orthology (KO) More...KOⁱ	K00134
OMAⁱ	FTLENMV
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2MZH

Family and domain databases

InterProⁱ	View protein in InterPro IPR020831 GlycerAld/Erythrose_P_DH IPR020830 GlycerAld_3-P_DH_AS IPR020829 GlycerAld_3-P_DH_cat IPR020828 GlycerAld_3-P_DH_NAD(P)-bd IPR006424 Glyceraldehyde-3-P_DH_1 IPR036291 NAD(P)-bd_dom_sf
PANTHERⁱ	PTHR10836 PTHR10836, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02800 Gp_dh_C, 1 hit PF00044 Gp_dh_N, 1 hit
PIRSFⁱ	PIRSF000149 GAP_DH, 1 hit
PRINTSⁱ	PR00078 G3PDHDRGNASE
SMARTⁱ	View protein in SMART SM00846 Gp_dh_N, 1 hit
SUPFAMⁱ	SSF51735 SSF51735, 1 hit
TIGRFAMsⁱ	TIGR01534 GAPDH-I, 1 hit
PROSITEⁱ	View protein in PROSITE PS00071 GAPDH, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P00360-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MIRIAINGFG RIGRLVLRLA LQRKDIEVVA VNDPFISNDY AAYMVKYDST 
        60         70         80         90        100
HGRYKGTVSH DDKHIIIDGV KIATYQERDP ANLPWGSLKI DVAVDSTGVF 
       110        120        130        140        150
KELDTAQKHI DAGAKKVVIT APSSSAPMFV VGVNHTKYTP DKKIVSNASC 
       160        170        180        190        200
TTNCLAPLAK VINDAFGIEE GLMTTVHSMT ATQKTVDGPS HKDWRGGRTA 
       210        220        230        240        250
SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLEKE 
       260        270        280        290        300
ATYDQIKKAV KAAAEGPMKG VLGYTEDAVV SSDFLGDTHA SIFDASAGIQ 
       310        320        330 
LSPKFVKLIS WYDNEYGYSA RVVDLIEYVA KA

Length:332

Mass (Da):35,750

Last modified:January 23, 2007 - v3

Checksum:ⁱ4C116C86EAF3DB70

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	248	E → A in CAA24609 (PubMed:6833300).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V01302 Genomic DNA Translation: CAA24609.1 Z49327 Genomic DNA Translation: CAA89343.1 AY693001 Genomic DNA Translation: AAT93020.1 BK006943 Genomic DNA Translation: DAA08747.1
PIRⁱ	S56824 DEBYG3
NCBI Reference Sequences More...RefSeqⁱ	NP_012483.3, NM_001181485.3

Genome annotation databases

EnsemblFungiⁱ	YJL052W; YJL052W; YJL052W
GeneIDⁱ	853395
KEGGⁱ	sce:YJL052W

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P00360	Glyceraldehyde-3-phosphate dehydrogenase		YEASK		332	UniRef100_P00360
Glyceraldehyde-3-phosphate dehydrogenase		YEASC		332

Protein	Similar proteins		Species	Score	Length	Source
P00360	Glyceraldehyde-3-phosphate dehydrogenase		YEASK		332	UniRef90_P00360
Glyceraldehyde-3-phosphate dehydrogenase		YEASC		332
Glyceraldehyde-3-phosphate dehydrogenase		SACEU		332
Glyceraldehyde-3-phosphate dehydrogenase		YEASB		332
Glyceraldehyde-3-phosphate dehydrogenase		YEAS1		332
+13

Protein	Similar proteins		Species	Score	Length	Source
P00360	Glyceraldehyde-3-phosphate dehydrogenase		RHIMI		336	UniRef50_P00360
Glyceraldehyde-3-phosphate dehydrogenase 2		SCHPO		335
Glyceraldehyde-3-phosphate dehydrogenase		BUCAP		332
Glyceraldehyde-3-phosphate dehydrogenase		ZYGRO		333
Glyceraldehyde-3-phosphate dehydrogenase		BUCAI		332
+2459

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V01302 Genomic DNA Translation: CAA24609.1 Z49327 Genomic DNA Translation: CAA89343.1 AY693001 Genomic DNA Translation: AAT93020.1 BK006943 Genomic DNA Translation: DAA08747.1
PIRⁱ	S56824 DEBYG3
RefSeqⁱ	NP_012483.3, NM_001181485.3

3D structure databases

ProteinModelPortalⁱ	P00360
SMRⁱ	P00360
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	33703, 76 interactors
DIPⁱ	DIP-4304N
IntActⁱ	P00360, 67 interactors
MINTⁱ	P00360
STRINGⁱ	4932.YJL052W

Protein family/group databases

MoonDBⁱ	P00360 Curated

MoonDBⁱ

P00360 Curated

PTM databases

iPTMnetⁱ	P00360

iPTMnetⁱ

P00360

2D gel databases

SWISS-2DPAGEⁱ	P00360

SWISS-2DPAGEⁱ

P00360

Proteomic databases

MaxQBⁱ	P00360
PaxDbⁱ	P00360
PRIDEⁱ	P00360
TopDownProteomicsⁱ	P00360

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	YJL052W; YJL052W; YJL052W
GeneIDⁱ	853395
KEGGⁱ	sce:YJL052W

Organism-specific databases

SGDⁱ	S000003588 TDH1

Phylogenomic databases

GeneTreeⁱ	ENSGT00760000119172
HOGENOMⁱ	HOG000071678
InParanoidⁱ	P00360
KOⁱ	K00134
OMAⁱ	FTLENMV
OrthoDBⁱ	EOG092C2MZH

Enzyme and pathway databases

UniPathwayⁱ	UPA00109;UER00184
BioCycⁱ	YEAST:YJL052W-MONOMER
SABIO-RKⁱ	P00360

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:P00360

PROⁱ

PR:P00360

Family and domain databases

InterProⁱ	View protein in InterPro IPR020831 GlycerAld/Erythrose_P_DH IPR020830 GlycerAld_3-P_DH_AS IPR020829 GlycerAld_3-P_DH_cat IPR020828 GlycerAld_3-P_DH_NAD(P)-bd IPR006424 Glyceraldehyde-3-P_DH_1 IPR036291 NAD(P)-bd_dom_sf
PANTHERⁱ	PTHR10836 PTHR10836, 1 hit
Pfamⁱ	View protein in Pfam PF02800 Gp_dh_C, 1 hit PF00044 Gp_dh_N, 1 hit
PIRSFⁱ	PIRSF000149 GAP_DH, 1 hit
PRINTSⁱ	PR00078 G3PDHDRGNASE
SMARTⁱ	View protein in SMART SM00846 Gp_dh_N, 1 hit
SUPFAMⁱ	SSF51735 SSF51735, 1 hit
TIGRFAMsⁱ	TIGR01534 GAPDH-I, 1 hit
PROSITEⁱ	View protein in PROSITE PS00071 GAPDH, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	G3P1_YEAST
Accessionⁱ	P00360Primary (citable) accession number: P00360 Secondary accession number(s): D6VWD1
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	January 23, 2007
	Last modified:	November 7, 2018
	This is version 194 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Direct protein sequencing, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
PATHWAY comments
Index of metabolic and biosynthesis pathways
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - P00360 (G3P1_YEAST)

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Glyceraldehyde-3-phosphate dehydrogenase 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolysis

Sites

Regions

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cell Wall

Cytosol

Mitochondrion

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

2D gel databases

PTM databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ