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Protein

Glyceraldehyde-3-phosphate dehydrogenase 3

Gene

TDH3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

There are three genes for G3PDH in yeast.
Present with 169000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3), Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1), Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase 1 (GPM1), Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 3 (GPM3)
  4. Enolase-related protein 3 (ERR3), Enolase-related protein 1 (ERR1), Enolase-related protein 2 (ERR2), Enolase 2 (ENO2), Enolase 1 (ENO1)
  5. Pyruvate kinase 1 (CDC19), Pyruvate kinase 2 (PYK2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NADBy similarity1
Binding sitei78NAD; via carbonyl oxygenBy similarity1
Active sitei150NucleophilePROSITE-ProRule annotation1
Sitei177Activates thiol group during catalysisBy similarity1
Binding sitei180Glyceraldehyde 3-phosphateBy similarity1
Binding sitei232Glyceraldehyde 3-phosphateBy similarity1
Binding sitei314NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADBy similarity2

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: SGD
  • gluconeogenesis Source: SGD
  • glycolytic process Source: SGD
  • heme transport Source: SGD
  • reactive oxygen species metabolic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD

Enzyme and pathway databases

BioCyciYEAST:YGR192C-MONOMER
ReactomeiR-SCE-70171 Glycolysis
R-SCE-70263 Gluconeogenesis
SABIO-RKiP00359
UniPathwayi
UPA00109;UER00184

Protein family/group databases

MoonDBiP00359 Curated
MoonProtiP00359

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 3 (EC:1.2.1.12)
Short name:
GAPDH 3
Gene namesi
Name:TDH3
Synonyms:GPD3
Ordered Locus Names:YGR192C
ORF Names:G7576
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

SGDiS000003424 TDH3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001455912 – 332Glyceraldehyde-3-phosphate dehydrogenase 3Add BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei302PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00359
PaxDbiP00359
PRIDEiP00359
TopDownProteomicsiP00359

2D gel databases

COMPLUYEAST-2DPAGEiP00359
SWISS-2DPAGEiP00359

PTM databases

CarbonylDBiP00359
iPTMnetiP00359

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi33445, 256 interactors
DIPiDIP-4309N
IntActiP00359, 102 interactors
MINTiP00359
STRINGi4932.YGR192C

Structurei

Secondary structure

1332
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00359
SMRiP00359
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni149 – 151Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni209 – 210Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00760000119172
HOGENOMiHOG000071678
InParanoidiP00359
KOiK00134
OMAiNCVAPMA
OrthoDBiEOG092C2MZH

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00359-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVRVAINGFG RIGRLVMRIA LSRPNVEVVA LNDPFITNDY AAYMFKYDST
60 70 80 90 100
HGRYAGEVSH DDKHIIVDGK KIATYQERDP ANLPWGSSNV DIAIDSTGVF
110 120 130 140 150
KELDTAQKHI DAGAKKVVIT APSSTAPMFV MGVNEEKYTS DLKIVSNASC
160 170 180 190 200
TTNCLAPLAK VINDAFGIEE GLMTTVHSLT ATQKTVDGPS HKDWRGGRTA
210 220 230 240 250
SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLNKE
260 270 280 290 300
TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSHS SIFDASAGIQ
310 320 330
LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA
Length:332
Mass (Da):35,747
Last modified:January 23, 2007 - v3
Checksum:i6CFFFEE7061BC36F
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti136E → V in CAA24607 (PubMed:385592).Curated1
Sequence conflicti136E → V in AAA88714 (PubMed:385592).Curated1
Sequence conflicti248N → D in CAA24607 (PubMed:385592).Curated1
Sequence conflicti248N → D in AAA88714 (PubMed:385592).Curated1
Sequence conflicti287D → G in AAS56157 (PubMed:17322287).Curated1
Sequence conflicti329V → I in CAA24607 (PubMed:385592).Curated1
Sequence conflicti329V → I in AAA88714 (PubMed:385592).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01300 Genomic DNA Translation: CAA24607.1
J01324 Genomic DNA Translation: AAA88714.1
X82408 Genomic DNA Translation: CAA57803.1
Z72977 Genomic DNA Translation: CAA97218.1
AY557831 Genomic DNA Translation: AAS56157.1
BK006941 Genomic DNA Translation: DAA08285.1
PIRiS55870 DEBYG2
RefSeqiNP_011708.3, NM_001181321.3

Genome annotation databases

EnsemblFungiiYGR192C; YGR192C; YGR192C
GeneIDi853106
KEGGisce:YGR192C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01300 Genomic DNA Translation: CAA24607.1
J01324 Genomic DNA Translation: AAA88714.1
X82408 Genomic DNA Translation: CAA57803.1
Z72977 Genomic DNA Translation: CAA97218.1
AY557831 Genomic DNA Translation: AAS56157.1
BK006941 Genomic DNA Translation: DAA08285.1
PIRiS55870 DEBYG2
RefSeqiNP_011708.3, NM_001181321.3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3PYMX-ray2.00A/B1-332[»]
4IQ8X-ray2.49A1-332[»]
ProteinModelPortaliP00359
SMRiP00359
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33445, 256 interactors
DIPiDIP-4309N
IntActiP00359, 102 interactors
MINTiP00359
STRINGi4932.YGR192C

Protein family/group databases

MoonDBiP00359 Curated
MoonProtiP00359

PTM databases

CarbonylDBiP00359
iPTMnetiP00359

2D gel databases

COMPLUYEAST-2DPAGEiP00359
SWISS-2DPAGEiP00359

Proteomic databases

MaxQBiP00359
PaxDbiP00359
PRIDEiP00359
TopDownProteomicsiP00359

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR192C; YGR192C; YGR192C
GeneIDi853106
KEGGisce:YGR192C

Organism-specific databases

SGDiS000003424 TDH3

Phylogenomic databases

GeneTreeiENSGT00760000119172
HOGENOMiHOG000071678
InParanoidiP00359
KOiK00134
OMAiNCVAPMA
OrthoDBiEOG092C2MZH

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00184

BioCyciYEAST:YGR192C-MONOMER
ReactomeiR-SCE-70171 Glycolysis
R-SCE-70263 Gluconeogenesis
SABIO-RKiP00359

Miscellaneous databases

PROiPR:P00359

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiG3P3_YEAST
AccessioniPrimary (citable) accession number: P00359
Secondary accession number(s): D6VUX4, Q6Q5P9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 200 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names
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Main funding by: National Institutes of Health

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