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Protein

Glyceraldehyde-3-phosphate dehydrogenase 2

Gene

TDH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

There are three genes for G3PDH in yeast.
Present with 121000 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3), Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1), Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase 1 (GPM1), Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 3 (GPM3)
  4. Enolase-related protein 3 (ERR3), Enolase-related protein 1 (ERR1), Enolase-related protein 2 (ERR2), Enolase 2 (ENO2), Enolase 1 (ENO1)
  5. Pyruvate kinase 1 (CDC19), Pyruvate kinase 2 (PYK2)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33NADBy similarity1
Binding sitei78NAD; via carbonyl oxygenBy similarity1
Active sitei150NucleophilePROSITE-ProRule annotation1
Sitei177Activates thiol group during catalysisBy similarity1
Binding sitei180Glyceraldehyde 3-phosphateBy similarity1
Binding sitei232Glyceraldehyde 3-phosphateBy similarity1
Binding sitei314NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi11 – 12NADBy similarity2

GO - Molecular functioni

GO - Biological processi

  • apoptotic process Source: SGD
  • gluconeogenesis Source: SGD
  • glycolytic process Source: SGD
  • reactive oxygen species metabolic process Source: SGD

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD

Enzyme and pathway databases

BioCyciYEAST:YJR009C-MONOMER
ReactomeiR-SCE-70171 Glycolysis
R-SCE-70263 Gluconeogenesis
SABIO-RKiP00358
UniPathwayi
UPA00109;UER00184

Protein family/group databases

MoonDBiP00358 Curated

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 2 (EC:1.2.1.12)
Short name:
GAPDH 2
Gene namesi
Name:TDH2
Synonyms:GPD2
Ordered Locus Names:YJR009C
ORF Names:J1433
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

SGDiS000003769 TDH2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001455902 – 332Glyceraldehyde-3-phosphate dehydrogenase 2Add BLAST331

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki46Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki63Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei302PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00358
PaxDbiP00358
PRIDEiP00358
TopDownProteomicsiP00358

2D gel databases

SWISS-2DPAGEiP00358

PTM databases

CarbonylDBiP00358
iPTMnetiP00358

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
UBI4P0CG632EBI-7212,EBI-7000452

Protein-protein interaction databases

BioGridi33765, 125 interactors
DIPiDIP-1951N
IntActiP00358, 61 interactors
MINTiP00358
STRINGi4932.YJR009C

Structurei

3D structure databases

ProteinModelPortaliP00358
SMRiP00358
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni149 – 151Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni209 – 210Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00930000152363
HOGENOMiHOG000071678
InParanoidiP00358
KOiK00134
OMAiKYDPSSM
OrthoDBiEOG092C2MZH

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00358-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVRVAINGFG RIGRLVMRIA LQRKNVEVVA LNDPFISNDY SAYMFKYDST
60 70 80 90 100
HGRYAGEVSH DDKHIIVDGH KIATFQERDP ANLPWASLNI DIAIDSTGVF
110 120 130 140 150
KELDTAQKHI DAGAKKVVIT APSSTAPMFV MGVNEEKYTS DLKIVSNASC
160 170 180 190 200
TTNCLAPLAK VINDAFGIEE GLMTTVHSMT ATQKTVDGPS HKDWRGGRTA
210 220 230 240 250
SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLNKE
260 270 280 290 300
TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSNS SIFDAAAGIQ
310 320 330
LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA
Length:332
Mass (Da):35,847
Last modified:January 23, 2007 - v3
Checksum:i9A99DCB26672541E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti77E → A AA sequence (PubMed:7737086).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60157 Genomic DNA Translation: CAA42725.1
V01301 Genomic DNA Translation: CAA24608.1 Sequence problems.
X87611 Genomic DNA Translation: CAA60931.1
Z49509 Genomic DNA Translation: CAA89531.1
BK006943 Genomic DNA Translation: DAA08800.1
PIRiS57024 DEBYG1
RefSeqiNP_012542.1, NM_001181666.1

Genome annotation databases

EnsemblFungiiYJR009C; YJR009C; YJR009C
GeneIDi853465
KEGGisce:YJR009C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60157 Genomic DNA Translation: CAA42725.1
V01301 Genomic DNA Translation: CAA24608.1 Sequence problems.
X87611 Genomic DNA Translation: CAA60931.1
Z49509 Genomic DNA Translation: CAA89531.1
BK006943 Genomic DNA Translation: DAA08800.1
PIRiS57024 DEBYG1
RefSeqiNP_012542.1, NM_001181666.1

3D structure databases

ProteinModelPortaliP00358
SMRiP00358
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33765, 125 interactors
DIPiDIP-1951N
IntActiP00358, 61 interactors
MINTiP00358
STRINGi4932.YJR009C

Protein family/group databases

MoonDBiP00358 Curated

PTM databases

CarbonylDBiP00358
iPTMnetiP00358

2D gel databases

SWISS-2DPAGEiP00358

Proteomic databases

MaxQBiP00358
PaxDbiP00358
PRIDEiP00358
TopDownProteomicsiP00358

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR009C; YJR009C; YJR009C
GeneIDi853465
KEGGisce:YJR009C

Organism-specific databases

SGDiS000003769 TDH2

Phylogenomic databases

GeneTreeiENSGT00930000152363
HOGENOMiHOG000071678
InParanoidiP00358
KOiK00134
OMAiKYDPSSM
OrthoDBiEOG092C2MZH

Enzyme and pathway databases

UniPathwayi
UPA00109;UER00184

BioCyciYEAST:YJR009C-MONOMER
ReactomeiR-SCE-70171 Glycolysis
R-SCE-70263 Gluconeogenesis
SABIO-RKiP00358

Miscellaneous databases

PROiPR:P00358

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020830 GlycerAld_3-P_DH_AS
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR10836 PTHR10836, 1 hit
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit
PROSITEiView protein in PROSITE
PS00071 GAPDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiG3P2_YEAST
AccessioniPrimary (citable) accession number: P00358
Secondary accession number(s): D6VWI4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: November 7, 2018
This is version 195 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names
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Main funding by: National Institutes of Health

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