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Protein

L-lactate dehydrogenase

Gene

ldh

Organism
Bacillus megaterium
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of lactate to pyruvate.UniRule annotation

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.UniRule annotation

Enzyme regulationi

Allosterically activated by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh), L-lactate dehydrogenase (ldh)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei20NAD; via amide nitrogenUniRule annotation1
Binding sitei41NADUniRule annotation1
Binding sitei46NADUniRule annotation1
Binding sitei71NADUniRule annotation1
Binding sitei88SubstrateUniRule annotation1
Binding sitei94SubstrateUniRule annotation1
Binding sitei149NADUniRule annotation1
Binding sitei159Allosteric activatorUniRule annotation1
Binding sitei174Allosteric activatorUniRule annotation1
Active sitei181Proton acceptorUniRule annotation1
Binding sitei235SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi85 – 86NADUniRule annotation2
Nucleotide bindingi124 – 126NADUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAllosteric enzyme, Oxidoreductase
LigandNAD

Enzyme and pathway databases

BRENDAi1.1.1.27 656
UniPathwayiUPA00554; UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenaseUniRule annotation (EC:1.1.1.27UniRule annotation)
Short name:
L-LDHUniRule annotation
Gene namesi
Name:ldhUniRule annotation
OrganismiBacillus megaterium
Taxonomic identifieri1404 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001683251 – 318L-lactate dehydrogenaseAdd BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei226PhosphotyrosineUniRule annotation1

Keywords - PTMi

Phosphoprotein

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliP00345
SMRiP00345
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni126 – 129Substrate bindingUniRule annotation4
Regioni154 – 157Substrate bindingUniRule annotation4

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4105C80 Bacteria
COG0039 LUCA

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

P00345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTQFTPKTR KVAVIGTGFV GSSYAFSMVN QGIANELVLI DMNKEKAEGE
60 70 80 90 100
ARDINHGMPF ATPMKIWAGD YKDCADADLA VITAGANQAP GETRLDLVEK
110 120 130 140 150
NVKIFECIVK DIMNSGFDGI ILVATNPVDI LAHVTQKVSG LPNGRVIGSG
160 170 180 190 200
TILDTARFRY LLSDYFEVDS RNVHAYIMGE HGDTEFPVWS HAQIGGVKLE
210 220 230 240 250
HFINTAAIEK EPDMQHLFEQ TRDAAYHIIN RKGATYYGIA MGLVRITKAI
260 270 280 290 300
LDDENSILTV SALLEGQYGI SDVYIGVPAI INKNGVRQII ELNLTPHEQQ
310
QLEHSASILK QTRDRAFV
Length:318
Mass (Da):35,035
Last modified:January 1, 1990 - v2
Checksum:i17122190576E1485
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M22305 Genomic DNA Translation: AAA22566.1
PIRiS00133 DEBSLM

Similar proteinsi

Entry informationi

Entry nameiLDH_BACME
AccessioniPrimary (citable) accession number: P00345
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 1, 1990
Last modified: May 23, 2018
This is version 113 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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