Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-lactate dehydrogenase A chain

Gene

ldha

Organism
Squalus acanthias (Spiny dogfish)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase B chain (ldhb), L-lactate dehydrogenase A chain (ldha)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NAD1 Publication1
Binding sitei107SubstrateBy similarity1
Binding sitei139NAD or substrateBy similarity1
Binding sitei170Substrate1
Active sitei194Proton acceptor1
Binding sitei249SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 58NAD1 PublicationAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

SABIO-RKiP00341
UniPathwayi
UPA00554;UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Alternative name(s):
LDH-M
Gene namesi
Name:ldha
OrganismiSqualus acanthias (Spiny dogfish)
Taxonomic identifieri7797 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataChondrichthyesElasmobranchiiSqualimorphiiSqualiformesSqualidaeSqualus

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001684552 – 333L-lactate dehydrogenase A chainAdd BLAST332

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PRIDEiP00341

PTM databases

iPTMnetiP00341

Interactioni

Subunit structurei

Homotetramer.1 Publication

Structurei

Secondary structure

1333
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00341
SMRiP00341
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00341

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

HOVERGENiHBG000462

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00341-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATLKDKLIG HLATSQEPRS YNKITVVGVG AVGMACAISI LMKDLADEVA
60 70 80 90 100
LVDVMEDKLK GEMMDLQHGS LFLHTAKIVS GKDYSVSAGS KLVVITAGAR
110 120 130 140 150
QQEGESRLNL VQRNVNIFKF IIPDIVKHSP DCIILVVSNP VDVLTYVAWK
160 170 180 190 200
LSGLPMHRII GSGCNLDSAR FRYLMGERLG VHSSSCHGWV IGEHGDSSVP
210 220 230 240 250
VWSGMNVAGV SLKELHPELG TDKDKENWKK LHKDVVDSAY EVIKLKGYTS
260 270 280 290 300
WAIGLSVADL AETIMKNLCR VHPVSTMVKD FYGIKNDVFL SLPCVLDNHG
310 320 330
ISNIVKMKLK PDEEQQLQKS ATTLWDIQKD LKF
Length:333
Mass (Da):36,695
Last modified:January 23, 2007 - v4
Checksum:i20C3B5F84B6C0117
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti124D → N AA sequence (PubMed:838743).Curated1
Sequence conflicti184S → C AA sequence (PubMed:838743).Curated1
Sequence conflicti198 – 200SVP → VPS AA sequence (PubMed:838743).Curated3
Sequence conflicti206 – 211NVAGVS → WNA AA sequence (PubMed:838743).Curated6
Sequence conflicti222D → N AA sequence (PubMed:838743).Curated1
Sequence conflicti226 – 227EN → QD AA sequence (PubMed:838743).Curated2
Sequence conflicti286 – 287ND → DN AA sequence (PubMed:838743).Curated2
Sequence conflicti297 – 298DN → ND AA sequence (PubMed:838743).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38893 mRNA Translation: AAA91038.1
PIRiA00350 DEDFLM

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U38893 mRNA Translation: AAA91038.1
PIRiA00350 DEDFLM

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1LDMX-ray2.10A2-333[»]
3LDHX-ray3.00A4-333[»]
6LDHX-ray2.00A2-333[»]
8LDHX-ray2.80A2-333[»]
ProteinModelPortaliP00341
SMRiP00341
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiP00341

Proteomic databases

PRIDEiP00341

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG000462

Enzyme and pathway databases

UniPathwayi
UPA00554;UER00611

SABIO-RKiP00341

Miscellaneous databases

EvolutionaryTraceiP00341

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLDHA_SQUAC
AccessioniPrimary (citable) accession number: P00341
Secondary accession number(s): Q92114
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 125 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again