Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

L-lactate dehydrogenase A chain

Gene

LDHA

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase (LDHBL), L-lactate dehydrogenase (LDHA), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase (LDHB)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99NADBy similarity1
Binding sitei106SubstrateBy similarity1
Binding sitei138NAD or substrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei193Proton acceptorBy similarity1
Binding sitei248SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi29 – 57NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

ReactomeiR-GGA-373920 Pyruvate metabolism
SABIO-RKiP00340
UniPathwayi
UPA00554;UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase A chain (EC:1.1.1.27)
Short name:
LDH-A
Gene namesi
Name:LDHA
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001684202 – 332L-lactate dehydrogenase A chainAdd BLAST331

Proteomic databases

PaxDbiP00340
PRIDEiP00340

PTM databases

iPTMnetiP00340

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi676481, 2 interactors
IntActiP00340, 1 interactor
STRINGi9031.ENSGALP00000010167

Structurei

3D structure databases

ProteinModelPortaliP00340
SMRiP00340
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495 Eukaryota
COG0039 LUCA
HOGENOMiHOG000213793
HOVERGENiHBG000462
InParanoidiP00340
KOiK00016
PhylomeDBiP00340

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00340-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSLKDHLIHN VHKEEHAHAH NKISVVGVGA VGMACAISIL MKDLADELTL
60 70 80 90 100
VDVVEDKLKG EMLDLQHGSL FLKTPKIISG KDYSVTAHSK LVIVTAGARQ
110 120 130 140 150
QEGESRLNLV QRNVNIFKFI IPNVVKYSPD CKLLIVSNPV DILTYVAWKI
160 170 180 190 200
SGFPKHRVIG SGCNLDSARF RHLMGERLGI HPLSCHGWIV GEHGDSSVPV
210 220 230 240 250
WSGVNVAGVS LKALHPDMGT DADKEHWKEV HKQVVDSAYE VIKLKGYTSW
260 270 280 290 300
AIGLSVADLA ETIMKNLRRV HPISTAVKGM HGIKDDVFLS VPCVLGSSGI
310 320 330
TDVVKMILKP DEEEKIKKSA DTLWGIQKEL QF
Length:332
Mass (Da):36,514
Last modified:January 23, 2007 - v3
Checksum:i5ED8279CFAD7B62B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63L → M AA sequence (Ref. 2) Curated1
Sequence conflicti78I → T AA sequence (Ref. 2) Curated1
Sequence conflicti192E → Q AA sequence (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53828 mRNA Translation: CAA37824.1
PIRiA00349 DECHLM
S12151
RefSeqiNP_990615.1, NM_205284.1
UniGeneiGga.4398

Genome annotation databases

GeneIDi396221
KEGGigga:396221

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53828 mRNA Translation: CAA37824.1
PIRiA00349 DECHLM
S12151
RefSeqiNP_990615.1, NM_205284.1
UniGeneiGga.4398

3D structure databases

ProteinModelPortaliP00340
SMRiP00340
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi676481, 2 interactors
IntActiP00340, 1 interactor
STRINGi9031.ENSGALP00000010167

PTM databases

iPTMnetiP00340

Proteomic databases

PaxDbiP00340
PRIDEiP00340

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi396221
KEGGigga:396221

Organism-specific databases

CTDi3939

Phylogenomic databases

eggNOGiKOG1495 Eukaryota
COG0039 LUCA
HOGENOMiHOG000213793
HOVERGENiHBG000462
InParanoidiP00340
KOiK00016
PhylomeDBiP00340

Enzyme and pathway databases

UniPathwayi
UPA00554;UER00611

ReactomeiR-GGA-373920 Pyruvate metabolism
SABIO-RKiP00340

Miscellaneous databases

PROiPR:P00340

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLDHA_CHICK
AccessioniPrimary (citable) accession number: P00340
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: October 25, 2017
This is version 132 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again