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Protein

L-lactate dehydrogenase B chain

Gene

LDHB

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase C chain (LDHC), L-lactate dehydrogenase A chain (LDHA), L-lactate dehydrogenase B chain (LDHB), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase, L-lactate dehydrogenase (LDHA), L-lactate dehydrogenase (LDHC), L-lactate dehydrogenase (LDHB), L-lactate dehydrogenase (LDHAL6B), L-lactate dehydrogenase, L-lactate dehydrogenase (LDHA), L-lactate dehydrogenase (LDHA), L-lactate dehydrogenase (LDHA), L-lactate dehydrogenase (LDHA), L-lactate dehydrogenase, L-lactate dehydrogenase (LDHA)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NADBy similarity1
Binding sitei107SubstrateBy similarity1
Binding sitei139NAD or substrateBy similarity1
Binding sitei170SubstrateBy similarity1
Active sitei194Proton acceptorBy similarity1
Binding sitei249SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 58NADBy similarityAdd BLAST29

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

SABIO-RKiP00336
UniPathwayi
UPA00554;UER00611

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase B chain (EC:1.1.1.27)
Short name:
LDH-B
Alternative name(s):
LDH heart subunit
Short name:
LDH-H
Gene namesi
Name:LDHB
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3823

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001684622 – 334L-lactate dehydrogenase B chainAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanine1 Publication1
Modified residuei7N6-acetyllysineBy similarity1
Modified residuei44PhosphoserineBy similarity1
Modified residuei58N6-acetyllysineBy similarity1
Modified residuei119N6-acetyllysineBy similarity1
Modified residuei240PhosphotyrosineBy similarity1
Modified residuei329N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00336
PeptideAtlasiP00336
PRIDEiP00336

PTM databases

iPTMnetiP00336

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000024847

Chemistry databases

BindingDBiP00336

Structurei

Secondary structure

1334
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00336
SMRiP00336
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00336

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495 Eukaryota
COG0039 LUCA
HOGENOMiHOG000213793
HOVERGENiHBG000462
InParanoidiP00336
KOiK00016

Family and domain databases

Gene3Di3.90.110.10, 1 hit
HAMAPiMF_00488 Lactate_dehydrog, 1 hit
InterProiView protein in InterPro
IPR001557 L-lactate/malate_DH
IPR011304 L-lactate_DH
IPR018177 L-lactate_DH_AS
IPR022383 Lactate/malate_DH_C
IPR001236 Lactate/malate_DH_N
IPR015955 Lactate_DH/Glyco_Ohase_4_C
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02866 Ldh_1_C, 1 hit
PF00056 Ldh_1_N, 1 hit
PIRSFiPIRSF000102 Lac_mal_DH, 1 hit
PRINTSiPR00086 LLDHDRGNASE
SUPFAMiSSF51735 SSF51735, 1 hit
SSF56327 SSF56327, 1 hit
TIGRFAMsiTIGR01771 L-LDH-NAD, 1 hit
PROSITEiView protein in PROSITE
PS00064 L_LDH, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.iShow all

P00336-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATLKEKLIA PVAEEETTIP NNKITVVGVG QVGMACAISI LGKSLTDELA
60 70 80 90 100
LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR
110 120 130 140 150
QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCIIIVVSNP VDILTYVTWK
160 170 180 190 200
LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG VHPSSCHGWI LGEHGDSSVA
210 220 230 240 250
VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVESAY EVIKLKGYTN
260 270 280 290 300
WAIGLSVADL IESMLKNLSR IHPVSTMVQG MYGIENEVFL SLPCVLNARG
310 320 330
LTSVINQKLK DDEVAQLKNS ADTLWGIQKD LKDL
Length:334
Mass (Da):36,612
Last modified:January 23, 2007 - v3
Checksum:iA0A2389BAB8FFD73
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F1SR05F1SR05_PIG
L-lactate dehydrogenase
LDHB
377Annotation score:
A0A287B094A0A287B094_PIG
L-lactate dehydrogenase
LDHB
332Annotation score:
A0A287AQS1A0A287AQS1_PIG
L-lactate dehydrogenase B chain
LDHB
319Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti14 – 15EE → QQ AA sequence (PubMed:838465).Curated2
Sequence conflicti81D → N AA sequence (PubMed:838465).Curated1
Sequence conflicti131D → N AA sequence (PubMed:838465).Curated1
Sequence conflicti214E → Q AA sequence (PubMed:838465).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07180 mRNA Translation: AAA50438.1
PIRiA91671 DEPGLH
RefSeqiNP_001106758.1, NM_001113287.1
UniGeneiSsc.48909

Genome annotation databases

GeneIDi100621540
KEGGissc:100621540

Similar proteinsi

Entry informationi

Entry nameiLDHB_PIG
AccessioniPrimary (citable) accession number: P00336
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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