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Protein

Alcohol dehydrogenase

Gene

Adh

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.PROSITE-ProRule annotation
A secondary alcohol + NAD+ = a ketone + NADH.PROSITE-ProRule annotation

Enzyme regulationi

Inhibited by 2,2,2-trifluoroethanol and pyrazole.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei65NAD1 Publication1
Binding sitei140Substrate1 Publication1
Active sitei153Proton acceptor1 Publication1
Binding sitei157NAD1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 41NAD1 PublicationAdd BLAST30

GO - Molecular functioni

  • acetaldehyde dehydrogenase (acetylating) activity Source: FlyBase
  • alcohol dehydrogenase (NAD) activity Source: FlyBase
  • protein homodimerization activity Source: FlyBase

GO - Biological processi

  • acetaldehyde metabolic process Source: FlyBase
  • alcohol catabolic process Source: UniProtKB
  • alcohol metabolic process Source: FlyBase
  • behavioral response to ethanol Source: FlyBase
  • ethanol metabolic process Source: FlyBase
  • ethanol oxidation Source: FlyBase
  • oxidation-reduction process Source: FlyBase

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

ReactomeiR-DME-2142700 Synthesis of Lipoxins (LX)
R-DME-9018676 Biosynthesis of D-series resolvins
R-DME-9018896 Biosynthesis of E-series 18(S)-resolvins
SABIO-RKiP00334

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase (EC:1.1.1.1)
Gene namesi
Name:Adh
ORF Names:CG3481
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0000055 Adh

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi15G → A: 31% decrease in activity. 1 Publication1
Mutagenesisi15G → D in Adh-N11; loss of activity. 1 Publication1
Mutagenesisi15G → V: Complete loss of activity. 1 Publication1
Mutagenesisi130G → C: Complete loss of activity. 1 Publication1
Mutagenesisi133G → I: Complete loss of activity. 1 Publication1
Mutagenesisi136C → A: No decrease in activity. 1 Publication1
Mutagenesisi153Y → C: Retains only 0.25% activity. 3 Publications1
Mutagenesisi153Y → F, H, E or Q: Complete loss of activity. 3 Publications1
Mutagenesisi157K → I: Complete loss of activity. 2 Publications1
Mutagenesisi157K → R: Retains only 2.2% activity. 2 Publications1
Mutagenesisi184G → L: Complete loss of activity. 1 Publication1
Mutagenesisi219C → A: No decrease in activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00000544742 – 256Alcohol dehydrogenaseAdd BLAST255

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP00334
PeptideAtlasiP00334
PRIDEiP00334

PTM databases

iPTMnetiP00334

Expressioni

Gene expression databases

BgeeiFBgn0000055
ExpressionAtlasiP00334 baseline and differential
GenevisibleiP00334 DM

Interactioni

Subunit structurei

Homodimer.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi533474, 61 interactors
IntActiP00334, 26 interactors
STRINGi7227.FBpp0100048

Structurei

Secondary structure

1256
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi9 – 13Combined sources5
Turni14 – 16Combined sources3
Helixi18 – 27Combined sources10
Beta strandi33 – 40Combined sources8
Helixi44 – 53Combined sources10
Beta strandi57 – 63Combined sources7
Helixi70 – 84Combined sources15
Beta strandi89 – 92Combined sources4
Helixi102 – 109Combined sources8
Helixi111 – 124Combined sources14
Helixi126 – 128Combined sources3
Beta strandi133 – 138Combined sources6
Helixi141 – 143Combined sources3
Helixi151 – 174Combined sources24
Beta strandi175 – 183Combined sources9
Beta strandi185 – 188Combined sources4
Turni189 – 192Combined sources4
Helixi197 – 199Combined sources3
Helixi204 – 209Combined sources6
Helixi216 – 229Combined sources14
Beta strandi235 – 239Combined sources5
Beta strandi242 – 245Combined sources4

3D structure databases

ProteinModelPortaliP00334
SMRiP00334
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00334

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4169 Eukaryota
COG1028 LUCA
InParanoidiP00334
KOiK00001
OrthoDBiEOG091G0OC9
PhylomeDBiP00334

Family and domain databases

InterProiView protein in InterPro
IPR002425 ADH_Drosophila-type
IPR002424 ADH_insect
IPR036291 NAD(P)-bd_dom_sf
IPR020904 Sc_DH/Rdtase_CS
IPR002347 SDR_fam
PfamiView protein in Pfam
PF00106 adh_short, 1 hit
PRINTSiPR01168 ALCDHDRGNASE
PR01167 INSADHFAMILY
PR00080 SDRFAMILY
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00061 ADH_SHORT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFTLTNKNV IFVAGLGGIG LDTSKELLKR DLKNLVILDR IENPAAIAEL
60 70 80 90 100
KAINPKVTVT FYPYDVTVPI AETTKLLKTI FAQLKTVDVL INGAGILDDH
110 120 130 140 150
QIERTIAVNY TGLVNTTTAI LDFWDKRKGG PGGIICNIGS VTGFNAIYQV
160 170 180 190 200
PVYSGTKAAV VNFTSSLAKL APITGVTAYT VNPGITRTTL VHKFNSWLDV
210 220 230 240 250
EPQVAEKLLA HPTQPSLACA ENFVKAIELN QNGAIWKLDL GTLEAIQWTK

HWDSGI
Length:256
Mass (Da):27,761
Last modified:January 23, 2007 - v2
Checksum:iEB4DF91029007284
GO

Polymorphismi

Virtually all natural populations of this species are polymorphic for 2 electrophoretically distinguishable alleles, Adh-S and Adh-F (PubMed:6789320, PubMed:6410283, PubMed:1673107, PubMed:1683848, Ref. 12, PubMed:12537569, Ref. 18, PubMed:6821373, PubMed:6780981). The sequence of the Adh-S allele is shown (PubMed:6789320, PubMed:6410283, PubMed:1673107, Ref. 12, PubMed:12537569, Ref. 18, PubMed:6821373). Other naturally occurring alleles include Adh-JA-F, Adh-AF-S, Adh-F-CHD, Adh-71K, Adh-UF and Adh-F' (PubMed:3021568, PubMed:3137352, PubMed:2124644, PubMed:6789328, PubMed:115502, PubMed:6821373). Artificially induced mutations include Adh-NB, Adh-NLA248, Adh-N4 and Adh-N11 (PubMed:6818527, PubMed:3928896, PubMed:3108863, PubMed:6821373, PubMed:2419573).18 Publications

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti9N → V in allele Adh-UF. 1
Natural varianti46A → D in allele Adh-UF. 1
Natural varianti46A → V in strain: NC16. 1
Natural varianti52A → E in allele Adh-F'. 1
Natural varianti71A → S in strain: NC16. 1
Natural varianti193K → T in allele Adh-F, allele Adh-F-CHD, allele Adh-71K, allele Adh-JA-F and in strain Berkeley. 1
Natural varianti215P → S in allele Adh-F-CHD and allele Adh-71K. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17827 Genomic DNA Translation: AAA28341.1
M17828 Genomic DNA Translation: AAA28342.1
M19547 Genomic DNA Translation: AAA70210.1
M17830 Genomic DNA Translation: AAA28343.1
M17831 Genomic DNA Translation: AAA28344.1
M17832 Genomic DNA Translation: AAA28345.1
M17833 Genomic DNA Translation: AAA28346.1
M17834 Genomic DNA Translation: AAA28347.1
M17835 Genomic DNA Translation: AAA28348.1
M17836 Genomic DNA Translation: AAA28349.1
M17837 Genomic DNA Translation: AAA70212.1
Z00030 Genomic DNA Translation: CAA77330.1
M22210 Genomic DNA Translation: AAA28338.1
M57239 Genomic DNA Translation: AAA28330.1
X78384 Genomic DNA Translation: CAA55151.1
M36580 Genomic DNA Translation: AAA28331.1
X60791 Genomic DNA Translation: CAA43204.1
X60792 Genomic DNA Translation: CAA43205.1
X60793 Genomic DNA Translation: CAA43206.1
U20765 Genomic DNA Translation: AAA88817.1
X98338 mRNA Translation: CAA66981.1
AF175211 Genomic DNA Translation: AAF00229.1
AF175212 Genomic DNA Translation: AAF00230.1
AF175213 Genomic DNA Translation: AAF00231.1
AF175214 Genomic DNA Translation: AAF00232.1
AF175215 Genomic DNA Translation: AAF00233.1
AF175216 Genomic DNA Translation: AAF00234.1
AF175217 Genomic DNA Translation: AAF00235.1
AF175218 Genomic DNA Translation: AAF00236.1
AF175219 Genomic DNA Translation: AAF00237.1
AF175220 Genomic DNA Translation: AAF00238.1
AE014134 Genomic DNA Translation: AAO41197.1
AE014134 Genomic DNA Translation: AAO41199.1
AE014134 Genomic DNA Translation: AAO41200.1
AE014134 Genomic DNA Translation: AAO41201.1
AY060227 mRNA Translation: AAL25266.1
BT012435 mRNA Translation: AAS93706.1
M17845 Genomic DNA Translation: AAA28363.1
PIRiA93309 DEFFA
RefSeqiNP_001027266.1, NM_001032095.2
NP_001027267.1, NM_001032096.2
NP_001027268.1, NM_001032097.2
NP_001027269.1, NM_001032098.2
NP_001027270.1, NM_001032099.2
UniGeneiDm.6818

Genome annotation databases

GeneIDi3771877
KEGGidme:Dmel_CG3481

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiADH_DROME
AccessioniPrimary (citable) accession number: P00334
Secondary accession number(s): A4V0Q1
, Q27332, Q27579, Q27596, Q2MGK2, Q53XD5, Q95TC8, Q9NKC0, Q9VJS3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 193 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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