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Protein

Alcohol dehydrogenase 2

Gene

ADH2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This isozyme preferentially catalyzes the conversion of ethanol to acetaldehyde. Acts on a variety of primary unbranched aliphatic alcohols.

Miscellaneous

Present with 1620 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.
A secondary alcohol + NAD+ = a ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Zinc 1; catalytic1
Metal bindingi67Zinc 1; catalytic1
Metal bindingi98Zinc 21
Metal bindingi101Zinc 21
Metal bindingi104Zinc 21
Metal bindingi112Zinc 21
Metal bindingi154Zinc 1; catalytic1
Binding sitei202NADBy similarity1
Binding sitei207NADBy similarity1
Binding sitei341NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 184NADBy similarity7
Nucleotide bindingi269 – 271NADBy similarity3

GO - Molecular functioni

  • alcohol dehydrogenase (NAD) activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
  • ethanol metabolic process Source: SGD
  • NADH oxidation Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YMR303C-MONOMER
YEAST:YMR303C-MONOMER
BRENDAi1.1.1.1 984

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 2 (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase II
YADH-2
Gene namesi
Name:ADH2
Synonyms:ADR2
Ordered Locus Names:YMR303C
ORF Names:YM9952.05C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR303C
SGDiS000004918 ADH2

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001607312 – 348Alcohol dehydrogenase 2Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei213PhosphoserineBy similarity1
Modified residuei223PhosphothreonineBy similarity1
Cross-linki226Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki234Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei279PhosphoserineBy similarity1
Cross-linki287Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei316PhosphoserineBy similarity1
Cross-linki319Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00331
PaxDbiP00331
PRIDEiP00331

PTM databases

CarbonylDBiP00331
iPTMnetiP00331

Expressioni

Inductioni

Repressed by glucose.

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

BioGridi35483, 73 interactors
DIPiDIP-1181N
IntActiP00331, 24 interactors
MINTiP00331
STRINGi4932.YMR303C

Structurei

3D structure databases

ProteinModelPortaliP00331
SMRiP00331
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075207
HOGENOMiHOG000294685
InParanoidiP00331
KOiK13953
OMAiARIPKEC
OrthoDBiEOG092C2Q8E

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00331-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIPETQKAI IFYESNGKLE HKDIPVPKPK PNELLINVKY SGVCHTDLHA
60 70 80 90 100
WHGDWPLPTK LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA
110 120 130 140 150
CEYCELGNES NCPHADLSGY THDGSFQEYA TADAVQAAHI PQGTDLAEVA
160 170 180 190 200
PILCAGITVY KALKSANLRA GHWAAISGAA GGLGSLAVQY AKAMGYRVLG
210 220 230 240 250
IDGGPGKEEL FTSLGGEVFI DFTKEKDIVS AVVKATNGGA HGIINVSVSE
260 270 280 290 300
AAIEASTRYC RANGTVVLVG LPAGAKCSSD VFNHVVKSIS IVGSYVGNRA
310 320 330 340
DTREALDFFA RGLVKSPIKV VGLSSLPEIY EKMEKGQIAG RYVVDTSK
Length:348
Mass (Da):36,732
Last modified:January 23, 2007 - v3
Checksum:i54535FC3258B10EA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti16N → H in AAA34411 (PubMed:6279086).Curated1
Sequence conflicti31P → A in AAA34411 (PubMed:6279086).Curated1
Sequence conflicti233V → K AA sequence (PubMed:7895733).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01314 Genomic DNA Translation: AAA34408.1
M38457 Genomic DNA Translation: AAA34411.1
Z49212 Genomic DNA Translation: CAA89136.1
BK006946 Genomic DNA Translation: DAA10204.1
PIRiA00340 DEBYA2
RefSeqiNP_014032.1, NM_001182812.1

Genome annotation databases

EnsemblFungiiYMR303C; YMR303C; YMR303C
GeneIDi855349
KEGGisce:YMR303C

Similar proteinsi

Entry informationi

Entry nameiADH2_YEAST
AccessioniPrimary (citable) accession number: P00331
Secondary accession number(s): D6W0D0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 181 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

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