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Protein

Alcohol dehydrogenase 1

Gene

ADH1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

This isozyme preferentially catalyzes the conversion of primary unbranched alcohols to their corresponding aldehydes. Also also shows activity toward secondary alcohols.

Miscellaneous

PubMed:320000 sequence has several conflicting residues and reports microheterogeneities at additional postitions. Analysis of the sequence suggests that the sequenced protein was a mixture of at least 3 of the different isoforms of alcohol dehydrogenases found in yeast.

Catalytic activityi

A primary alcohol + NAD+ = an aldehyde + NADH.
A secondary alcohol + NAD+ = a ketone + NADH.

Cofactori

Zn2+Note: Binds 2 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi44Zinc 1; catalytic1
Metal bindingi67Zinc 1; catalytic1
Metal bindingi98Zinc 21
Metal bindingi101Zinc 21
Metal bindingi104Zinc 21
Metal bindingi112Zinc 21
Metal bindingi154Zinc 1; catalytic1
Binding sitei202NADBy similarity1
Binding sitei207NADBy similarity1
Binding sitei341NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi178 – 184NADBy similarity7
Nucleotide bindingi269 – 271NADBy similarity3

GO - Molecular functioni

  • alcohol dehydrogenase (NAD) activity Source: SGD
  • identical protein binding Source: IntAct
  • methylglyoxal reductase (NADH-dependent) activity Source: SGD
  • zinc ion binding Source: InterPro

GO - Biological processi

  • amino acid catabolic process to alcohol via Ehrlich pathway Source: SGD
  • ethanol biosynthetic process involved in glucose fermentation to ethanol Source: SGD
  • ethanol metabolic process Source: GO_Central
  • NADH oxidation Source: SGD

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:YOL086C-MONOMER
YEAST:YOL086C-MONOMER
BRENDAi1.1.1.1 984
SABIO-RKiP00330

Protein family/group databases

MoonDBiP00330 Curated

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase 1 (EC:1.1.1.1)
Alternative name(s):
Alcohol dehydrogenase I
YADH-1
Gene namesi
Name:ADH1
Synonyms:ADC1
Ordered Locus Names:YOL086C
ORF Names:O0947
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

SGDiS000005446 ADH1

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001607302 – 348Alcohol dehydrogenase 1Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserine1 Publication1
Modified residuei213PhosphoserineCombined sources1
Modified residuei223PhosphothreonineCombined sources1
Cross-linki226Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Cross-linki234Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei279PhosphoserineCombined sources1
Cross-linki287Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei316PhosphoserineCombined sources1
Cross-linki319Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP00330
PaxDbiP00330
PRIDEiP00330
TopDownProteomicsiP00330

2D gel databases

COMPLUYEAST-2DPAGEiP00330
SWISS-2DPAGEiP00330

PTM databases

iPTMnetiP00330
SwissPalmiP00330

Interactioni

Subunit structurei

Homotetramer.

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-2218,EBI-2218

GO - Molecular functioni

Protein-protein interaction databases

BioGridi34317, 213 interactors
DIPiDIP-1143N
IntActiP00330, 215 interactors
MINTiP00330
STRINGi4932.YOL086C

Structurei

Secondary structure

1348
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00330
SMRiP00330
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00330

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00550000075207
HOGENOMiHOG000294685
InParanoidiP00330
KOiK13953
OMAiAYCKSEV
OrthoDBiEOG092C2Q8E

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00330-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSIPETQKGV IFYESHGKLE YKDIPVPKPK ANELLINVKY SGVCHTDLHA
60 70 80 90 100
WHGDWPLPVK LPLVGGHEGA GVVVGMGENV KGWKIGDYAG IKWLNGSCMA
110 120 130 140 150
CEYCELGNES NCPHADLSGY THDGSFQQYA TADAVQAAHI PQGTDLAQVA
160 170 180 190 200
PILCAGITVY KALKSANLMA GHWVAISGAA GGLGSLAVQY AKAMGYRVLG
210 220 230 240 250
IDGGEGKEEL FRSIGGEVFI DFTKEKDIVG AVLKATDGGA HGVINVSVSE
260 270 280 290 300
AAIEASTRYV RANGTTVLVG MPAGAKCCSD VFNQVVKSIS IVGSYVGNRA
310 320 330 340
DTREALDFFA RGLVKSPIKV VGLSTLPEIY EKMEKGQIVG RYVVDTSK
Length:348
Mass (Da):36,849
Last modified:October 5, 2010 - v5
Checksum:iF14AE24B5D8D12A5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21Y → H in CAA24601 (PubMed:6277922).Curated1
Sequence conflicti21Y → H in AAA34410 (PubMed:6279086).Curated1
Sequence conflicti59V → T AA sequence (PubMed:320000).Curated1
Sequence conflicti148Q → E AA sequence (PubMed:320000).Curated1
Sequence conflicti152I → V AA sequence (PubMed:320000).Curated1
Sequence conflicti237D → N AA sequence (PubMed:320000).Curated1
Sequence conflicti314V → I AA sequence (PubMed:320000).Curated1
Sequence conflicti338I → V AA sequence (PubMed:320000).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti236T → I1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01292 Genomic DNA Translation: CAA24601.1
M38456 Genomic DNA Translation: AAA34410.1
X83121 Genomic DNA Translation: CAA58193.1
Z74828 Genomic DNA Translation: CAA99098.1
V01291 Genomic DNA Translation: CAA24600.1
BK006948 Genomic DNA Translation: DAA10699.1
PIRiS57383 DEBYA
RefSeqiNP_014555.1, NM_001183340.1

Genome annotation databases

EnsemblFungiiYOL086C; YOL086C; YOL086C
GeneIDi854068
KEGGisce:YOL086C

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V01292 Genomic DNA Translation: CAA24601.1
M38456 Genomic DNA Translation: AAA34410.1
X83121 Genomic DNA Translation: CAA58193.1
Z74828 Genomic DNA Translation: CAA99098.1
V01291 Genomic DNA Translation: CAA24600.1
BK006948 Genomic DNA Translation: DAA10699.1
PIRiS57383 DEBYA
RefSeqiNP_014555.1, NM_001183340.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4W6ZX-ray2.40A/B/C/D2-348[»]
5ENVX-ray3.00A/B2-348[»]
ProteinModelPortaliP00330
SMRiP00330
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34317, 213 interactors
DIPiDIP-1143N
IntActiP00330, 215 interactors
MINTiP00330
STRINGi4932.YOL086C

Protein family/group databases

MoonDBiP00330 Curated

PTM databases

iPTMnetiP00330
SwissPalmiP00330

2D gel databases

COMPLUYEAST-2DPAGEiP00330
SWISS-2DPAGEiP00330

Proteomic databases

MaxQBiP00330
PaxDbiP00330
PRIDEiP00330
TopDownProteomicsiP00330

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL086C; YOL086C; YOL086C
GeneIDi854068
KEGGisce:YOL086C

Organism-specific databases

SGDiS000005446 ADH1

Phylogenomic databases

GeneTreeiENSGT00550000075207
HOGENOMiHOG000294685
InParanoidiP00330
KOiK13953
OMAiAYCKSEV
OrthoDBiEOG092C2Q8E

Enzyme and pathway databases

BioCyciMetaCyc:YOL086C-MONOMER
YEAST:YOL086C-MONOMER
BRENDAi1.1.1.1 984
SABIO-RKiP00330

Miscellaneous databases

EvolutionaryTraceiP00330
PROiPR:P00330

Family and domain databases

InterProiView protein in InterPro
IPR013149 ADH_C
IPR013154 ADH_N
IPR002328 ADH_Zn_CS
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
PfamiView protein in Pfam
PF08240 ADH_N, 1 hit
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 1 hit
SSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00059 ADH_ZINC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiADH1_YEAST
AccessioniPrimary (citable) accession number: P00330
Secondary accession number(s): D6W1Y3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: October 5, 2010
Last modified: November 7, 2018
This is version 198 of the entry and version 5 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
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