UniProtKB - P00325 (ADH1B_HUMAN)
Protein
All-trans-retinol dehydrogenase [NAD(+)] ADH1B
Gene
ADH1B
Organism
Homo sapiens (Human)
Status
Functioni
Catalyzes the NAD-dependent oxidation of all-trans-retinol and its derivatives such as all-trans-4-hydroxyretinol and may participate in retinoid metabolism (PubMed:15369820, PubMed:16787387). In vitro can also catalyzes the NADH-dependent reduction of all-trans-retinal and its derivatives such as all-trans-4-oxoretinal (PubMed:15369820, PubMed:16787387). Catalyzes in the oxidative direction with higher efficiency (PubMed:16787387). Has the same affinity for all-trans-4-hydroxyretinol and all-trans-4-oxoretinal (PubMed:15369820).2 Publications
Miscellaneous
There are 7 different ADH's isozymes in human: three belongs to class-I: alpha, beta, and gamma, one to class-II: pi, one to class-III: chi, one to class-IV: ADH7 and one to class-V: ADH6.
Catalytic activityi
- EC:1.1.1.1051 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forward1 Publication direction.
Cofactori
Zn2+Note: Binds 2 Zn2+ ions per subunit.
Kineticsi
- KM=4 µM for all-trans-4-hydroxyretinol (in allele ADH1B*1)1 Publication
- KM=25 µM for all-trans-4-oxoretinal (in allele ADH1B*1)1 Publication
- KM=11 µM for all-trans-4-hydroxyretinol (in allele ADH1B*2)1 Publication
- KM=27 µM for all-trans-4-oxoretinal (in allele ADH1B*2)1 Publication
- KM=24 µM for all-trans-3,4-didehydroretinol(in allele ADH1B*2)1 Publication
- KM=25 µM for all-trans-3,4-didehydroretinal(in allele ADH1B*2)1 Publication
- KM=0.4 µM for all-trans-retinaldehyde (in allele ADH1B*2)1 Publication
- KM=0.3 µM for all-trans-retinol (in allele ADH1B*2)1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 47 | Zinc 1; catalytic | 1 | |
Metal bindingi | 68 | Zinc 1; catalytic | 1 | |
Metal bindingi | 98 | Zinc 2 | 1 | |
Metal bindingi | 101 | Zinc 2 | 1 | |
Metal bindingi | 104 | Zinc 2 | 1 | |
Metal bindingi | 112 | Zinc 2 | 1 | |
Metal bindingi | 175 | Zinc 1; catalytic | 1 | |
Binding sitei | 224 | NAD | 1 | |
Binding sitei | 229 | NAD | 1 | |
Binding sitei | 370 | NAD | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 200 – 205 | NAD | 6 | |
Nucleotide bindingi | 293 – 295 | NAD | 3 |
GO - Molecular functioni
- alcohol dehydrogenase activity, zinc-dependent Source: UniProtKB
- retinol dehydrogenase activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- ethanol oxidation Source: UniProtKB
- retinoic acid metabolic process Source: GO_Central
- retinoid metabolic process Source: UniProtKB
- retinol metabolic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Ligand | Metal-binding, NAD, Zinc |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER66-321 |
PathwayCommonsi | P00325 |
Reactomei | R-HSA-71384, Ethanol oxidation |
SABIO-RKi | P00325 |
Chemistry databases
SwissLipidsi | SLP:000001880 |
Names & Taxonomyi
Protein namesi | Recommended name: All-trans-retinol dehydrogenase [NAD(+)] ADH1BCurated (EC:1.1.1.1051 Publication)Alternative name(s): Alcohol dehydrogenase 1B Alcohol dehydrogenase subunit beta |
Gene namesi | |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:250, ADH1B |
MIMi | 103720, gene+phenotype |
neXtProti | NX_P00325 |
VEuPathDBi | HostDB:ENSG00000196616.12 |
Subcellular locationi
Other locations
Cytosol
- cytosol Source: HPA
Nucleus
- nucleoplasm Source: HPA
Plasma Membrane
- plasma membrane Source: HPA
Keywords - Cellular componenti
CytoplasmPathology & Biotechi
Organism-specific databases
DisGeNETi | 125 |
MalaCardsi | ADH1B |
MIMi | 103720, gene+phenotype |
PharmGKBi | PA24571 |
Miscellaneous databases
Pharosi | P00325, Tclin |
Chemistry databases
ChEMBLi | CHEMBL3284 |
DrugBanki | DB02721, 4-Iodopyrazole DB03703, Cyclohexanol DB00898, Ethanol DB01213, Fomepizole DB09462, Glycerin DB02481, N-Benzylformamide DB04105, N-Heptylformamide DB00157, NADH DB03461, Nicotinamide adenine dinucleotide phosphate |
DrugCentrali | P00325 |
Genetic variation databases
BioMutai | ADH1B |
DMDMi | 113394 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000160661 | 2 – 375 | All-trans-retinol dehydrogenase [NAD(+)] ADH1BAdd BLAST | 374 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserine1 Publication | 1 | |
Modified residuei | 23 | PhosphoserineCombined sources | 1 | |
Modified residuei | 35 | PhosphotyrosineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | P00325 |
jPOSTi | P00325 |
MassIVEi | P00325 |
PaxDbi | P00325 |
PeptideAtlasi | P00325 |
PRIDEi | P00325 |
ProteomicsDBi | 2413 51228 [P00325-1] |
PTM databases
iPTMneti | P00325 |
PhosphoSitePlusi | P00325 |
Expressioni
Gene expression databases
Bgeei | ENSG00000196616, Expressed in adipose tissue and 203 other tissues |
ExpressionAtlasi | P00325, baseline and differential |
Genevisiblei | P00325, HS |
Organism-specific databases
HPAi | ENSG00000196616, Group enriched (adipose tissue, liver) |
Interactioni
Subunit structurei
Dimer of identical or non-identical chains of three types; alpha, beta and gamma.
Protein-protein interaction databases
BioGRIDi | 106637, 7 interactors |
IntActi | P00325, 11 interactors |
MINTi | P00325 |
STRINGi | 9606.ENSP00000306606 |
Chemistry databases
BindingDBi | P00325 |
Miscellaneous databases
RNActi | P00325, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | P00325 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00325 |
Family & Domainsi
Sequence similaritiesi
Belongs to the zinc-containing alcohol dehydrogenase family.Curated
Phylogenomic databases
eggNOGi | KOG0022, Eukaryota |
HOGENOMi | CLU_026673_14_0_1 |
InParanoidi | P00325 |
OrthoDBi | 664798at2759 |
PhylomeDBi | P00325 |
TreeFami | TF300429 |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: P00325-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSTAGKVIKC KAAVLWEVKK PFSIEDVEVA PPKAYEVRIK MVAVGICRTD
60 70 80 90 100
DHVVSGNLVT PLPVILGHEA AGIVESVGEG VTTVKPGDKV IPLFTPQCGK
110 120 130 140 150
CRVCKNPESN YCLKNDLGNP RGTLQDGTRR FTCRGKPIHH FLGTSTFSQY
160 170 180 190 200
TVVDENAVAK IDAASPLEKV CLIGCGFSTG YGSAVNVAKV TPGSTCAVFG
210 220 230 240 250
LGGVGLSAVM GCKAAGAARI IAVDINKDKF AKAKELGATE CINPQDYKKP
260 270 280 290 300
IQEVLKEMTD GGVDFSFEVI GRLDTMMASL LCCHEACGTS VIVGVPPASQ
310 320 330 340 350
NLSINPMLLL TGRTWKGAVY GGFKSKEGIP KLVADFMAKK FSLDALITHV
360 370
LPFEKINEGF DLLHSGKSIR TVLTF
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketV9HW50 | V9HW50_HUMAN | All-trans-retinol dehydrogenase [NA... | ADH1B HEL-S-117 | 375 | Annotation score: | ||
D6RHZ6 | D6RHZ6_HUMAN | All-trans-retinol dehydrogenase [NA... | ADH1B | 335 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 8 | I → M in AAA51592 (PubMed:3397059).Curated | 1 | |
Sequence conflicti | 130 | Missing AA sequence (PubMed:6391920).Curated | 1 | |
Sequence conflicti | 166 | P → K in AAA51592 (PubMed:3397059).Curated | 1 | |
Sequence conflicti | 190 | V → VV in CAA33487 (PubMed:2547609).Curated | 1 | |
Sequence conflicti | 220 | I → V in BAG62635 (PubMed:14702039).Curated | 1 | |
Sequence conflicti | 230 | F → K in AAA51884 (PubMed:2986130).Curated | 1 | |
Sequence conflicti | 235 | E → V in AAA51592 (PubMed:3397059).Curated | 1 | |
Sequence conflicti | 338 – 344 | Missing in BAG62635 (PubMed:14702039).Curated | 7 |
Polymorphismi
Three alleles are known: ADH1B*1 (ADH2*1) corresponding to variant beta-1, ADH1B*2 (ADH2*2) corresponding to variant beta-2, ADH1B*3 (ADH2*3) corresponding to variant beta-3. The sequence shown is that of allele ADH1B*1. The ADH1B*2 allele frequency in orientals is approximately 75%, whereas it is less than 5% in most Caucasian populations. ADH1B variations have been associated with protection against alcohol dependence and alcohol-related aerodigestive tract cancer [MIMi:103720].5 Publications
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_000426 | 48 | R → H in beta-2; allele ADH1B*2; common in Asian populations; associated with a lower risk of alcoholism. Combined sources7 PublicationsCorresponds to variant dbSNP:rs1229984Ensembl. | 1 | |
Natural variantiVAR_019322 | 57 | N → K2 PublicationsCorresponds to variant dbSNP:rs1041969Ensembl. | 1 | |
Natural variantiVAR_019323 | 60 | T → S1 PublicationCorresponds to variant dbSNP:rs6413413Ensembl. | 1 | |
Natural variantiVAR_000427 | 370 | R → C in beta-3/Indianapolis; allele ADH1B*3; decreased NAD(H) binding. 3 PublicationsCorresponds to variant dbSNP:rs2066702EnsemblClinVar. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_054847 | 1 – 40 | Missing in isoform 2. 1 PublicationAdd BLAST | 40 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24317 mRNA Translation: AAA51884.1 X03350 mRNA Translation: CAA27056.1 M24316 , M24308, M24309, M24310, M24311, M24312, M24313, M24314 Genomic DNA Translation: AAB59496.1 D00137 mRNA Translation: BAA00084.1 L38290 , L38283, L38284, L38285, L38286, L38287, L38288, L38289 Genomic DNA Translation: AAB48003.1 X15447 , X15448, X15449, X15450, X15451, X15452, X15453, X15454, X15455 Genomic DNA Translation: CAA33487.1 AF153821 mRNA Translation: AAD37446.1 DQ017646 Genomic DNA Translation: AAY22180.1 AK301018 mRNA Translation: BAG62635.1 AC097530 Genomic DNA No translation available. BC033009 mRNA Translation: AAH33009.1 M21692 mRNA Translation: AAA51592.1 AF040967 Genomic DNA Translation: AAB96912.1 |
CCDSi | CCDS34033.1 [P00325-1] CCDS68761.1 [P00325-2] |
PIRi | A23607, DEHUAB |
RefSeqi | NP_000659.2, NM_000668.5 NP_001273579.1, NM_001286650.1 |
Genome annotation databases
Ensembli | ENST00000305046; ENSP00000306606; ENSG00000196616 |
GeneIDi | 125 |
KEGGi | hsa:125 |
UCSCi | uc011cei.3, human [P00325-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Web resourcesi
NIEHS-SNPs |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M24317 mRNA Translation: AAA51884.1 X03350 mRNA Translation: CAA27056.1 M24316 , M24308, M24309, M24310, M24311, M24312, M24313, M24314 Genomic DNA Translation: AAB59496.1 D00137 mRNA Translation: BAA00084.1 L38290 , L38283, L38284, L38285, L38286, L38287, L38288, L38289 Genomic DNA Translation: AAB48003.1 X15447 , X15448, X15449, X15450, X15451, X15452, X15453, X15454, X15455 Genomic DNA Translation: CAA33487.1 AF153821 mRNA Translation: AAD37446.1 DQ017646 Genomic DNA Translation: AAY22180.1 AK301018 mRNA Translation: BAG62635.1 AC097530 Genomic DNA No translation available. BC033009 mRNA Translation: AAH33009.1 M21692 mRNA Translation: AAA51592.1 AF040967 Genomic DNA Translation: AAB96912.1 |
CCDSi | CCDS34033.1 [P00325-1] CCDS68761.1 [P00325-2] |
PIRi | A23607, DEHUAB |
RefSeqi | NP_000659.2, NM_000668.5 NP_001273579.1, NM_001286650.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1DEH | X-ray | 2.20 | A/B | 2-375 | [»] | |
1HDX | X-ray | 2.50 | A/B | 2-375 | [»] | |
1HDY | X-ray | 2.50 | A/B | 2-375 | [»] | |
1HDZ | X-ray | 2.50 | A/B | 2-375 | [»] | |
1HSZ | X-ray | 2.20 | A/B | 2-375 | [»] | |
1HTB | X-ray | 2.40 | A/B | 2-375 | [»] | |
1U3U | X-ray | 1.60 | A/B | 2-375 | [»] | |
1U3V | X-ray | 1.65 | A/B | 2-375 | [»] | |
3HUD | X-ray | 3.20 | A/B | 2-375 | [»] | |
SMRi | P00325 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 106637, 7 interactors |
IntActi | P00325, 11 interactors |
MINTi | P00325 |
STRINGi | 9606.ENSP00000306606 |
Chemistry databases
BindingDBi | P00325 |
ChEMBLi | CHEMBL3284 |
DrugBanki | DB02721, 4-Iodopyrazole DB03703, Cyclohexanol DB00898, Ethanol DB01213, Fomepizole DB09462, Glycerin DB02481, N-Benzylformamide DB04105, N-Heptylformamide DB00157, NADH DB03461, Nicotinamide adenine dinucleotide phosphate |
DrugCentrali | P00325 |
SwissLipidsi | SLP:000001880 |
PTM databases
iPTMneti | P00325 |
PhosphoSitePlusi | P00325 |
Genetic variation databases
BioMutai | ADH1B |
DMDMi | 113394 |
Proteomic databases
EPDi | P00325 |
jPOSTi | P00325 |
MassIVEi | P00325 |
PaxDbi | P00325 |
PeptideAtlasi | P00325 |
PRIDEi | P00325 |
ProteomicsDBi | 2413 51228 [P00325-1] |
Protocols and materials databases
Antibodypediai | 14861, 273 antibodies |
Genome annotation databases
Ensembli | ENST00000305046; ENSP00000306606; ENSG00000196616 |
GeneIDi | 125 |
KEGGi | hsa:125 |
UCSCi | uc011cei.3, human [P00325-1] |
Organism-specific databases
CTDi | 125 |
DisGeNETi | 125 |
GeneCardsi | ADH1B |
HGNCi | HGNC:250, ADH1B |
HPAi | ENSG00000196616, Group enriched (adipose tissue, liver) |
MalaCardsi | ADH1B |
MIMi | 103720, gene+phenotype |
neXtProti | NX_P00325 |
PharmGKBi | PA24571 |
VEuPathDBi | HostDB:ENSG00000196616.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG0022, Eukaryota |
HOGENOMi | CLU_026673_14_0_1 |
InParanoidi | P00325 |
OrthoDBi | 664798at2759 |
PhylomeDBi | P00325 |
TreeFami | TF300429 |
Enzyme and pathway databases
BioCyci | MetaCyc:MONOMER66-321 |
PathwayCommonsi | P00325 |
Reactomei | R-HSA-71384, Ethanol oxidation |
SABIO-RKi | P00325 |
Miscellaneous databases
BioGRID-ORCSi | 125, 1 hit in 849 CRISPR screens |
ChiTaRSi | ADH1B, human |
EvolutionaryTracei | P00325 |
GeneWikii | ADH1B |
GenomeRNAii | 125 |
Pharosi | P00325, Tclin |
PROi | PR:P00325 |
RNActi | P00325, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196616, Expressed in adipose tissue and 203 other tissues |
ExpressionAtlasi | P00325, baseline and differential |
Genevisiblei | P00325, HS |
Family and domain databases
InterProi | View protein in InterPro IPR013149, ADH_C IPR013154, ADH_N IPR002328, ADH_Zn_CS IPR011032, GroES-like_sf IPR036291, NAD(P)-bd_dom_sf IPR020843, PKS_ER |
Pfami | View protein in Pfam PF08240, ADH_N, 1 hit PF00107, ADH_zinc_N, 1 hit |
SMARTi | View protein in SMART SM00829, PKS_ER, 1 hit |
SUPFAMi | SSF50129, SSF50129, 2 hits SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00059, ADH_ZINC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ADH1B_HUMAN | |
Accessioni | P00325Primary (citable) accession number: P00325 Secondary accession number(s): A8MYN5 Q96KI7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 213 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Human chromosome 4
Human chromosome 4: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families