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Protein

Flavodoxin 2

Gene

nifF

Organism
Azotobacter vinelandii
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Flavodoxins are low-potential electron donors to a number of redox enzymes. NifF is the electron donor to nitrogenase, and is thus implicated in nitrogen fixation. Does not function as an electron donor to nitrite reductase.1 Publication

Cofactori

FMN2 Publications

Redox potential

E0 is -493 mV. It is the mid-point potential of the semiquinone/hydroquinone redox couple (SQ/HQ) of AvFld 2.1 Publication

Manual assertion based on experiment ini

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57FMN; via carbonyl oxygenCombined sources1 Publication1
Binding sitei61FMN; via amide nitrogenCombined sources1 Publication1
Binding sitei99FMN; via amide nitrogenCombined sources1 Publication1
Binding sitei155FMNCombined sources1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi10 – 15FMNCombined sources1 Publication6
Nucleotide bindingi106 – 108FMNCombined sources1 Publication3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Nitrogen fixation, Transport
LigandFlavoprotein, FMN

Names & Taxonomyi

Protein namesi
Recommended name:
Flavodoxin 21 Publication
Alternative name(s):
AvFld 21 Publication
Flavodoxin II1 Publication
Gene namesi
Name:nifF1 Publication
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved2 Publications
ChainiPRO_00001716052 – 180Flavodoxin 2Add BLAST179

Expressioni

Inductioni

Is the predominant flavodoxin expressed when A.vinelandii is grown on N2 as nitrogen source. Is also synthesized during growth on nitrate, but in less abundance than AvFld 1.1 Publication

Structurei

Secondary structure

1180
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 8Combined sources5
Beta strandi11 – 13Combined sources3
Helixi14 – 23Combined sources10
Turni28 – 30Combined sources3
Helixi37 – 39Combined sources3
Helixi42 – 46Combined sources5
Beta strandi49 – 56Combined sources8
Turni59 – 61Combined sources3
Helixi66 – 68Combined sources3
Helixi75 – 82Combined sources8
Beta strandi91 – 97Combined sources7
Turni100 – 102Combined sources3
Turni104 – 108Combined sources5
Helixi109 – 120Combined sources12
Beta strandi124 – 126Combined sources3
Beta strandi146 – 152Combined sources7
Turni154 – 156Combined sources3
Helixi158 – 160Combined sources3
Helixi161 – 176Combined sources16

3D structure databases

ProteinModelPortaliP00324
SMRiP00324
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00324

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 173Flavodoxin-likePROSITE-ProRule annotationAdd BLAST170

Sequence similaritiesi

Belongs to the flavodoxin family.Curated

Phylogenomic databases

eggNOGiENOG4108TGK Bacteria
COG0716 LUCA

Family and domain databases

Gene3Di3.40.50.360, 1 hit
InterProiView protein in InterPro
IPR001094 Flavdoxin-like
IPR008254 Flavodoxin/NO_synth
IPR001226 Flavodoxin_CS
IPR010086 Flavodoxin_lc
IPR029039 Flavoprotein-like_sf
PfamiView protein in Pfam
PF00258 Flavodoxin_1, 1 hit
PIRSFiPIRSF038996 FldA, 1 hit
PRINTSiPR00369 FLAVODOXIN
SUPFAMiSSF52218 SSF52218, 1 hit
TIGRFAMsiTIGR01752 flav_long, 1 hit
PROSITEiView protein in PROSITE
PS00201 FLAVODOXIN, 1 hit
PS50902 FLAVODOXIN_LIKE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P00324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKIGLFFGS NTGKTRKVAK SIKKRFDDET MSDALNVNRV SAEDFAQYQF
60 70 80 90 100
LILGTPTLGE GELPGLSSDC ENESWEEFLP KIEGLDFSGK TVALFGLGDQ
110 120 130 140 150
VGYPENYLDA LGELYSFFKD RGAKIVGSWS TDGYEFESSE AVVDGKFVGL
160 170 180
ALDLDNQSGK TDERVAAWLA QIAPEFGLSL
Length:180
Mass (Da):19,663
Last modified:January 23, 2007 - v2
Checksum:i8B1B43F23AB5E8B4
GO

Mass spectrometryi

Molecular mass is 19533±5 Da from positions 2 - 180. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20568 Genomic DNA Translation: AAA64735.1
J03519 Genomic DNA Translation: AAA22154.1
PIRiA29935 FXAVEP
RefSeqiWP_012698862.1, NZ_FPKM01000020.1

Similar proteinsi

Entry informationi

Entry nameiFLAV_AZOVI
AccessioniPrimary (citable) accession number: P00324
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: December 20, 2017
This is version 104 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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