UniProtKB - P00173 (CYB5_RAT)
Protein
Cytochrome b5
Gene
Cyb5a
Organism
Rattus norvegicus (Rat)
Status
Functioni
Cytochrome b5 is a membrane-bound hemoprotein functioning as an electron carrier for several membrane-bound oxygenases. It is also involved in several steps of the sterol biosynthesis pathway, particularly in the C-6 double bond introduction during the C-6 desaturation.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 44 | Iron (heme axial ligand) | 1 | |
Metal bindingi | 68 | Iron (heme axial ligand) | 1 |
GO - Molecular functioni
- electron transfer activity Source: GO_Central
- enzyme binding Source: RGD
- heme binding Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- response to cadmium ion Source: RGD
Keywordsi
Biological process | Electron transport, Transport |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-RNO-196836, Vitamin C (ascorbate) metabolism R-RNO-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome b5 |
Gene namesi | Name:Cyb5a Synonyms:Cyb5 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620558, Cyb5a |
Subcellular locationi
Endoplasmic reticulum
Cytosol
- cytosol Source: Ensembl
Endoplasmic reticulum
- endoplasmic reticulum Source: HGNC-UCL
- endoplasmic reticulum membrane Source: RGD
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: RGD
- membrane Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 109 – 131 | HelicalSequence analysisAdd BLAST | 23 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000166014 | 2 – 134 | Cytochrome b5Add BLAST | 133 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 7 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 10 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 19 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | P00173 |
PaxDbi | P00173 |
PRIDEi | P00173 |
PTM databases
iPTMneti | P00173 |
PhosphoSitePlusi | P00173 |
Expressioni
Gene expression databases
Bgeei | ENSRNOG00000015205, Expressed in liver and 21 other tissues |
Genevisiblei | P00173, RN |
Interactioni
GO - Molecular functioni
- enzyme binding Source: RGD
Protein-protein interaction databases
IntActi | P00173, 1 interactor |
STRINGi | 10116.ENSRNOP00000020446 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P00173 |
SMRi | P00173 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00173 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 85 | Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST | 77 |
Sequence similaritiesi
Belongs to the cytochrome b5 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0537, Eukaryota |
GeneTreei | ENSGT00940000156770 |
HOGENOMi | CLU_102602_3_3_1 |
InParanoidi | P00173 |
OMAi | WTNFLIP |
OrthoDBi | 1566561at2759 |
PhylomeDBi | P00173 |
TreeFami | TF314537 |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit |
PRINTSi | PR00363, CYTOCHROMEB5 |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform Long (identifier: P00173-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAEQSDKDVK YYTLEEIQKH KDSKSTWVIL HHKVYDLTKF LEEHPGGEEV
60 70 80 90 100
LREQAGGDAT ENFEDVGHST DARELSKTYI IGELHPDDRS KIAKPSETLI
110 120 130
TTVESNSSWW TNWVIPAISA LVVALMYRLY MAED
Isoform Short (identifier: P00173-2) [UniParc]FASTAAdd to basket
The sequence of this isoform differs from the canonical sequence as follows:
97-100: ETLI → HSAL
101-134: Missing.
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 18 | Q → E AA sequence (PubMed:6840088).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_001246 | 97 – 100 | ETLI → HSAL in isoform Short. Curated | 4 | |
Alternative sequenceiVSP_001247 | 101 – 134 | Missing in isoform Short. CuratedAdd BLAST | 34 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13205 mRNA Translation: BAA02492.1 AF007107 mRNA Translation: AAB67609.1 AF007108 mRNA Translation: AAB67610.1 BC086945 mRNA Translation: AAH86945.1 |
PIRi | JC5596 S28404, CBRT5 |
RefSeqi | NP_071581.1, NM_022245.1 [P00173-1] |
Genome annotation databases
Ensembli | ENSRNOT00000020446; ENSRNOP00000020446; ENSRNOG00000015205 [P00173-1] |
GeneIDi | 64001 |
KEGGi | rno:64001 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | D13205 mRNA Translation: BAA02492.1 AF007107 mRNA Translation: AAB67609.1 AF007108 mRNA Translation: AAB67610.1 BC086945 mRNA Translation: AAH86945.1 |
PIRi | JC5596 S28404, CBRT5 |
RefSeqi | NP_071581.1, NM_022245.1 [P00173-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1AQA | NMR | - | A | 6-99 | [»] | |
1AW3 | NMR | - | A | 6-99 | [»] | |
1AXX | NMR | - | A | 6-99 | [»] | |
1B5A | NMR | - | A | 6-99 | [»] | |
1B5B | NMR | - | A | 6-99 | [»] | |
1BFX | NMR | - | A | 1-99 | [»] | |
1BLV | NMR | - | A | 6-99 | [»] | |
1I87 | NMR | - | A | 2-99 | [»] | |
1I8C | NMR | - | A | 2-99 | [»] | |
1IB7 | NMR | - | A | 6-99 | [»] | |
1IET | NMR | - | A | 2-99 | [»] | |
1IEU | NMR | - | A | 2-99 | [»] | |
1JEX | NMR | - | A | 6-99 | [»] | |
1MNY | NMR | - | A | 6-99 | [»] | |
2AXX | NMR | - | A | 6-99 | [»] | |
5XE4 | NMR | - | A | 2-99 | [»] | |
5XEE | NMR | - | A | 2-99 | [»] | |
BMRBi | P00173 | |||||
SMRi | P00173 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
IntActi | P00173, 1 interactor |
STRINGi | 10116.ENSRNOP00000020446 |
PTM databases
iPTMneti | P00173 |
PhosphoSitePlusi | P00173 |
Proteomic databases
jPOSTi | P00173 |
PaxDbi | P00173 |
PRIDEi | P00173 |
Genome annotation databases
Ensembli | ENSRNOT00000020446; ENSRNOP00000020446; ENSRNOG00000015205 [P00173-1] |
GeneIDi | 64001 |
KEGGi | rno:64001 |
Organism-specific databases
CTDi | 1528 |
RGDi | 620558, Cyb5a |
Phylogenomic databases
eggNOGi | KOG0537, Eukaryota |
GeneTreei | ENSGT00940000156770 |
HOGENOMi | CLU_102602_3_3_1 |
InParanoidi | P00173 |
OMAi | WTNFLIP |
OrthoDBi | 1566561at2759 |
PhylomeDBi | P00173 |
TreeFami | TF314537 |
Enzyme and pathway databases
Reactomei | R-RNO-196836, Vitamin C (ascorbate) metabolism R-RNO-9609523, Insertion of tail-anchored proteins into the endoplasmic reticulum membrane |
Miscellaneous databases
EvolutionaryTracei | P00173 |
PROi | PR:P00173 |
Gene expression databases
Bgeei | ENSRNOG00000015205, Expressed in liver and 21 other tissues |
Genevisiblei | P00173, RN |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR018506, Cyt_B5_heme-BS |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit |
PRINTSi | PR00363, CYTOCHROMEB5 |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS00191, CYTOCHROME_B5_1, 1 hit PS50255, CYTOCHROME_B5_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYB5_RAT | |
Accessioni | P00173Primary (citable) accession number: P00173 Secondary accession number(s): O35768 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 180 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families