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Protein

Cytochrome b

Gene

COB

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.2 Publications

Miscellaneous

Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.By similarity

Caution

The protein contains only eight transmembrane helices, not nine as predicted by bioinformatics tools.2 Publications

Cofactori

heme2 PublicationsNote: Binds 2 heme groups non-covalently.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi82Iron 1 (heme b562 axial ligand)Combined sources2 Publications1
Metal bindingi96Iron 2 (heme b566 axial ligand)Combined sources2 Publications1
Metal bindingi183Iron 1 (heme b562 axial ligand)Combined sources2 Publications1
Metal bindingi197Iron 2 (heme b566 axial ligand)Combined sources2 Publications1
Binding sitei202UbiquinoneBy similarity1

GO - Molecular functioni

GO - Biological processi

  • aerobic respiration Source: SGD
  • mitochondrial electron transport, ubiquinol to cytochrome c Source: UniProtKB

Keywordsi

Biological processElectron transport, Respiratory chain, Transport
LigandHeme, Iron, Metal-binding, Ubiquinone

Enzyme and pathway databases

BioCyciYEAST:Q0105-MONOMER

Protein family/group databases

TCDBi3.D.3.3.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b
Alternative name(s):
Complex III subunit 3
Complex III subunit CYTB
Complex III subunit III
Cytochrome b-c1 complex subunit 3
Cytochrome b-c1 complex subunit CYTB
Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
Gene namesi
Name:COB
Synonyms:CYTB
Ordered Locus Names:Q0105
Encoded oniMitochondrion
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Mitochondrion

Organism-specific databases

EuPathDBiFungiDB:Q0105
SGDiS000007270 COB

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei32 – 52HelicalCombined sources2 PublicationsAdd BLAST21
Transmembranei76 – 98HelicalCombined sources2 PublicationsAdd BLAST23
Transmembranei113 – 133HelicalCombined sources2 PublicationsAdd BLAST21
Transmembranei179 – 199HelicalCombined sources2 PublicationsAdd BLAST21
Transmembranei225 – 245HelicalCombined sources2 PublicationsAdd BLAST21
Transmembranei289 – 309HelicalCombined sources2 PublicationsAdd BLAST21
Transmembranei321 – 341HelicalCombined sources2 PublicationsAdd BLAST21
Transmembranei348 – 368HelicalCombined sources2 PublicationsAdd BLAST21

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000617691 – 385Cytochrome bAdd BLAST385

Proteomic databases

PaxDbiP00163
PRIDEiP00163
TopDownProteomicsiP00163

Interactioni

Subunit structurei

Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.3 Publications

Protein-protein interaction databases

BioGridi34779, 33 interactors
ComplexPortaliCPX-567 Mitochondrial electron transport complex III
IntActiP00163, 3 interactors
STRINGi4932.Q0105

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00163
SMRiP00163
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00163

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00390000017948
HOGENOMiHOG000255206
InParanoidiP00163
KOiK00412
OMAiHETGSNN
OrthoDBiEOG092C2QFL

Family and domain databases

CDDicd00290 cytochrome_b_C, 1 hit
cd00284 Cytochrome_b_N, 1 hit
Gene3Di1.20.810.10, 1 hit
InterProiView protein in InterPro
IPR005798 Cyt_b/b6_C
IPR036150 Cyt_b/b6_C_sf
IPR005797 Cyt_b/b6_N
IPR027387 Cytb/b6-like_sf
IPR030689 Cytochrome_b
IPR016174 Di-haem_cyt_TM
PfamiView protein in Pfam
PF00032 Cytochrom_B_C, 1 hit
PF00033 Cytochrome_B, 1 hit
PIRSFiPIRSF038885 COB, 1 hit
SUPFAMiSSF81342 SSF81342, 1 hit
SSF81648 SSF81648, 1 hit
PROSITEiView protein in PROSITE
PS51003 CYTB_CTER, 1 hit
PS51002 CYTB_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

P00163-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAFRKSNVYL SLVNSYIIDS PQPSSINYWW NMGSLLGLCL VIQIVTGIFM
60 70 80 90 100
AMHYSSNIEL AFSSVEHIMR DVHNGYILRY LHANGASFFF MVMFMHMAKG
110 120 130 140 150
LYYGSYRSPR VTLWNVGVII FILTIATAFL GYCCVYGQMS HWGATVITNL
160 170 180 190 200
FSAIPFVGND IVSWLWGGFS VSNPTIQRFF ALHYLVPFII AAMVIMHLMA
210 220 230 240 250
LHIHGSSNPL GITGNLDRIP MHSYFIFKDL VTVFLFMLIL ALFVFYSPNT
260 270 280 290 300
LGHPDNYIPG NPLVTPASIV PEWYLLPFYA ILRSIPDKLL GVITMFAAIL
310 320 330 340 350
VLLVLPFTDR SVVRGNTFKV LSKFFFFIFV FNFVLLGQIG ACHVEVPYVL
360 370 380
MGQIATFIYF AYFLIIVPVI STIENVLFYI GRVNK
Length:385
Mass (Da):43,656
Last modified:April 23, 2003 - v3
Checksum:i1B1B1616BBC2A798
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti122I → T in CAA58861 (PubMed:7737175).Curated1
Sequence conflicti122I → T in AAA32151 (PubMed:7004642).Curated1
Sequence conflicti269I → ID in CAA24073 (PubMed:6253454).Curated1
Sequence conflicti269I → ID no nucleotide entry (PubMed:6383504).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti131G → S in mutant W7 which is respiratory deficient. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00696 Genomic DNA Translation: CAA24073.2 Sequence problems.
X84042 mRNA Translation: CAA58861.1
KP263414 Genomic DNA Translation: AIZ98890.1
J01473, J01472 Genomic DNA Translation: AAA32151.2
J01475, J01474 Genomic DNA Translation: AAA32152.2
PIRiA00159 CBBY
S78660
RefSeqiNP_009315.1, NC_001224.1

Genome annotation databases

EnsemblFungiiQ0105; Q0105; Q0105
GeneIDi854583
KEGGisce:Q0105

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00696 Genomic DNA Translation: CAA24073.2 Sequence problems.
X84042 mRNA Translation: CAA58861.1
KP263414 Genomic DNA Translation: AIZ98890.1
J01473, J01472 Genomic DNA Translation: AAA32151.2
J01475, J01474 Genomic DNA Translation: AAA32152.2
PIRiA00159 CBBY
S78660
RefSeqiNP_009315.1, NC_001224.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1EZVX-ray2.30C1-385[»]
1KB9X-ray2.30C1-385[»]
1KYOX-ray2.97C/N1-385[»]
1P84X-ray2.50C1-385[»]
2IBZX-ray2.30C1-385[»]
3CX5X-ray1.90C/N1-385[»]
3CXHX-ray2.50C/N1-385[»]
4PD4X-ray3.04C1-385[»]
ProteinModelPortaliP00163
SMRiP00163
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34779, 33 interactors
ComplexPortaliCPX-567 Mitochondrial electron transport complex III
IntActiP00163, 3 interactors
STRINGi4932.Q0105

Protein family/group databases

TCDBi3.D.3.3.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Proteomic databases

PaxDbiP00163
PRIDEiP00163
TopDownProteomicsiP00163

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiQ0105; Q0105; Q0105
GeneIDi854583
KEGGisce:Q0105

Organism-specific databases

EuPathDBiFungiDB:Q0105
SGDiS000007270 COB

Phylogenomic databases

GeneTreeiENSGT00390000017948
HOGENOMiHOG000255206
InParanoidiP00163
KOiK00412
OMAiHETGSNN
OrthoDBiEOG092C2QFL

Enzyme and pathway databases

BioCyciYEAST:Q0105-MONOMER

Miscellaneous databases

EvolutionaryTraceiP00163
PROiPR:P00163

Family and domain databases

CDDicd00290 cytochrome_b_C, 1 hit
cd00284 Cytochrome_b_N, 1 hit
Gene3Di1.20.810.10, 1 hit
InterProiView protein in InterPro
IPR005798 Cyt_b/b6_C
IPR036150 Cyt_b/b6_C_sf
IPR005797 Cyt_b/b6_N
IPR027387 Cytb/b6-like_sf
IPR030689 Cytochrome_b
IPR016174 Di-haem_cyt_TM
PfamiView protein in Pfam
PF00032 Cytochrom_B_C, 1 hit
PF00033 Cytochrome_B, 1 hit
PIRSFiPIRSF038885 COB, 1 hit
SUPFAMiSSF81342 SSF81342, 1 hit
SSF81648 SSF81648, 1 hit
PROSITEiView protein in PROSITE
PS51003 CYTB_CTER, 1 hit
PS51002 CYTB_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYB_YEAST
AccessioniPrimary (citable) accession number: P00163
Secondary accession number(s): A0A0A7NYK0
, Q35802, Q35807, Q36301, Q9ZZW9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: April 23, 2003
Last modified: July 18, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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