UniProtKB - P00163 (CYB_YEAST)

BLAST

>sp|P00163|CYB_YEAST Cytochrome b OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=COB PE=1 SV=3
MAFRKSNVYLSLVNSYIIDSPQPSSINYWWNMGSLLGLCLVIQIVTGIFMAMHYSSNIEL
AFSSVEHIMRDVHNGYILRYLHANGASFFFMVMFMHMAKGLYYGSYRSPRVTLWNVGVII
FILTIATAFLGYCCVYGQMSHWGATVITNLFSAIPFVGNDIVSWLWGGFSVSNPTIQRFF
ALHYLVPFIIAAMVIMHLMALHIHGSSNPLGITGNLDRIPMHSYFIFKDLVTVFLFMLIL
ALFVFYSPNTLGHPDNYIPGNPLVTPASIVPEWYLLPFYAILRSIPDKLLGVITMFAAIL
VLLVLPFTDRSVVRGNTFKVLSKFFFFIFVFNFVLLGQIGACHVEVPYVLMGQIATFIYF
AYFLIIVPVISTIENVLFYIGRVNK

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History
Entry version 166 (18 Jul 2018)
Sequence version 3 (23 Apr 2003)
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Protein

Cytochrome b

Gene

COB

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.2 Publications

Miscellaneous

Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.By similarity

Caution

The protein contains only eight transmembrane helices, not nine as predicted by bioinformatics tools.2 Publications

Cofactorⁱ

heme2 PublicationsNote: Binds 2 heme groups non-covalently.2 Publications

Sites

Feature key	Position(s)	DescriptionActions	Length
Metal bindingⁱ	82	Iron 1 (heme b562 axial ligand)Combined sources2 Publications	1
Metal bindingⁱ	96	Iron 2 (heme b566 axial ligand)Combined sources2 Publications	1
Metal bindingⁱ	183	Iron 1 (heme b562 axial ligand)Combined sources2 Publications	1
Metal bindingⁱ	197	Iron 2 (heme b566 axial ligand)Combined sources2 Publications	1
Binding siteⁱ	202	UbiquinoneBy similarity	1

GO - Molecular functionⁱ

electron transporter, transferring electrons within CoQH2-cytochrome c reductase complex activity
Source: SGD
Inferred from direct assayⁱ
- 391561
metal ion binding Source: UniProtKB-KW
ubiquinol-cytochrome-c reductase activity
Source: UniProtKB
Inferred from direct assayⁱ
- 8031140

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

aerobic respiration
Source: SGD
Inferred from mutant phenotypeⁱ
- 391561
mitochondrial electron transport, ubiquinol to cytochrome c
Source: UniProtKB
Inferred from direct assayⁱ
- 8031140

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Electron transport, Respiratory chain, Transport
Ligand	Heme, Iron, Metal-binding, Ubiquinone

Enzyme and pathway databases

BioCycⁱ	YEAST:Q0105-MONOMER

BioCycⁱ

YEAST:Q0105-MONOMER

Protein family/group databases

TCDBⁱ	3.D.3.3.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

TCDBⁱ

3.D.3.3.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Cytochrome b Alternative name(s): Complex III subunit 3 Complex III subunit CYTB Complex III subunit III Cytochrome b-c1 complex subunit 3 Cytochrome b-c1 complex subunit CYTB Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
Gene namesⁱ	Name:COB Synonyms:CYTB Ordered Locus Names:Q0105
Encoded onⁱ	Mitochondrion
Organismⁱ	Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifierⁱ	559292 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae
Proteomesⁱ	UP000002311 Componentⁱ: Mitochondrion

Organism-specific databases

EuPathDBⁱ	FungiDB:Q0105
Saccharomyces Genome Database More...SGDⁱ	S000007270 COB

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	32 – 52	HelicalCombined sources2 PublicationsAdd BLAST	21
Transmembraneⁱ	76 – 98	HelicalCombined sources2 PublicationsAdd BLAST	23
Transmembraneⁱ	113 – 133	HelicalCombined sources2 PublicationsAdd BLAST	21
Transmembraneⁱ	179 – 199	HelicalCombined sources2 PublicationsAdd BLAST	21
Transmembraneⁱ	225 – 245	HelicalCombined sources2 PublicationsAdd BLAST	21
Transmembraneⁱ	289 – 309	HelicalCombined sources2 PublicationsAdd BLAST	21
Transmembraneⁱ	321 – 341	HelicalCombined sources2 PublicationsAdd BLAST	21
Transmembraneⁱ	348 – 368	HelicalCombined sources2 PublicationsAdd BLAST	21

Keywords - Cellular componentⁱ

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000061769	1 – 385	Cytochrome bAdd BLAST		385

Proteomic databases

PaxDbⁱ	P00163
PRIDEⁱ	P00163
TopDownProteomicsⁱ	P00163

Interactionⁱ

Subunit structureⁱ

Fungal cytochrome b-c1 complex contains 10 subunits; 3 respiratory subunits, 2 core proteins and 5 low-molecular weight proteins. Cytochrome b-c1 complex is a homodimer.3 Publications

Protein-protein interaction databases

BioGridⁱ	34779, 33 interactors
ComplexPortalⁱ	CPX-567 Mitochondrial electron transport complex III
IntActⁱ	P00163, 3 interactors
STRINGⁱ	4932.Q0105

Structureⁱ

Secondary structure

Legend: HelixTurnBeta strandPDB Structure known for this area

Feature key	Position(s)	DescriptionActions	Length
Helixⁱ	3 – 6	Combined sources	4
Helixⁱ	10 – 17	Combined sources	8
Beta strandⁱ	21 – 23	Combined sources	3
Helixⁱ	28 – 31	Combined sources	4
Helixⁱ	32 – 51	Combined sources	20
Turnⁱ	58 – 60	Combined sources	3
Helixⁱ	61 – 70	Combined sources	10
Helixⁱ	75 – 102	Combined sources	28
Turnⁱ	103 – 106	Combined sources	4
Turnⁱ	108 – 110	Combined sources	3
Helixⁱ	111 – 135	Combined sources	25
Helixⁱ	138 – 149	Combined sources	12
Helixⁱ	150 – 153	Combined sources	4
Turnⁱ	155 – 157	Combined sources	3
Helixⁱ	158 – 166	Combined sources	9
Beta strandⁱ	168 – 171	Combined sources	4
Helixⁱ	173 – 204	Combined sources	32
Beta strandⁱ	217 – 220	Combined sources	4
Turnⁱ	221 – 223	Combined sources	3
Helixⁱ	224 – 246	Combined sources	23
Turnⁱ	248 – 251	Combined sources	4
Helixⁱ	254 – 257	Combined sources	4
Helixⁱ	273 – 275	Combined sources	3
Helixⁱ	276 – 283	Combined sources	8
Beta strandⁱ	285 – 287	Combined sources	3
Helixⁱ	288 – 300	Combined sources	13
Helixⁱ	301 – 304	Combined sources	4
Helixⁱ	305 – 308	Combined sources	4
Beta strandⁱ	312 – 316	Combined sources	5
Helixⁱ	320 – 340	Combined sources	21
Helixⁱ	348 – 364	Combined sources	17
Helixⁱ	366 – 380	Combined sources	15

Show more details

3D structure databases

ProteinModelPortalⁱ	P00163
SMRⁱ	P00163
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Miscellaneous databases

EvolutionaryTraceⁱ

P00163

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the cytochrome b family.PROSITE-ProRule annotation

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000017948
HOGENOMⁱ	HOG000255206
InParanoidⁱ	P00163
KEGG Orthology (KO) More...KOⁱ	K00412
OMAⁱ	HETGSNN
Database of Orthologous Groups More...OrthoDBⁱ	EOG092C2QFL

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00290 cytochrome_b_C, 1 hit cd00284 Cytochrome_b_N, 1 hit
Gene3Dⁱ	1.20.810.10, 1 hit
InterProⁱ	View protein in InterPro IPR005798 Cyt_b/b6_C IPR036150 Cyt_b/b6_C_sf IPR005797 Cyt_b/b6_N IPR027387 Cytb/b6-like_sf IPR030689 Cytochrome_b IPR016174 Di-haem_cyt_TM
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00032 Cytochrom_B_C, 1 hit PF00033 Cytochrome_B, 1 hit
PIRSFⁱ	PIRSF038885 COB, 1 hit
SUPFAMⁱ	SSF81342 SSF81342, 1 hit SSF81648 SSF81648, 1 hit
PROSITEⁱ	View protein in PROSITE PS51003 CYTB_CTER, 1 hit PS51002 CYTB_NTER, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

P00163-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAFRKSNVYL SLVNSYIIDS PQPSSINYWW NMGSLLGLCL VIQIVTGIFM 
        60         70         80         90        100
AMHYSSNIEL AFSSVEHIMR DVHNGYILRY LHANGASFFF MVMFMHMAKG 
       110        120        130        140        150
LYYGSYRSPR VTLWNVGVII FILTIATAFL GYCCVYGQMS HWGATVITNL 
       160        170        180        190        200
FSAIPFVGND IVSWLWGGFS VSNPTIQRFF ALHYLVPFII AAMVIMHLMA 
       210        220        230        240        250
LHIHGSSNPL GITGNLDRIP MHSYFIFKDL VTVFLFMLIL ALFVFYSPNT 
       260        270        280        290        300
LGHPDNYIPG NPLVTPASIV PEWYLLPFYA ILRSIPDKLL GVITMFAAIL 
       310        320        330        340        350
VLLVLPFTDR SVVRGNTFKV LSKFFFFIFV FNFVLLGQIG ACHVEVPYVL 
       360        370        380 
MGQIATFIYF AYFLIIVPVI STIENVLFYI GRVNK

Length:385

Mass (Da):43,656

Last modified:April 23, 2003 - v3

Checksum:ⁱ1B1B1616BBC2A798

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	122	I → T in CAA58861 (PubMed:7737175).Curated	1
Sequence conflictⁱ	122	I → T in AAA32151 (PubMed:7004642).Curated	1
Sequence conflictⁱ	269	I → ID in CAA24073 (PubMed:6253454).Curated	1
Sequence conflictⁱ	269	I → ID no nucleotide entry (PubMed:6383504).Curated	1

Natural variant

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Natural variantⁱ	131	G → S in mutant W7 which is respiratory deficient.		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V00696 Genomic DNA Translation: CAA24073.2 Sequence problems. X84042 mRNA Translation: CAA58861.1 KP263414 Genomic DNA Translation: AIZ98890.1 J01473, J01472 Genomic DNA Translation: AAA32151.2 J01475, J01474 Genomic DNA Translation: AAA32152.2
PIRⁱ	A00159 CBBY S78660
NCBI Reference Sequences More...RefSeqⁱ	NP_009315.1, NC_001224.1

Genome annotation databases

EnsemblFungiⁱ	Q0105; Q0105; Q0105
GeneIDⁱ	854583
KEGGⁱ	sce:Q0105

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
P00163	Cytochrome b		YEASX		385	UniRef100_P00163

Protein	Similar proteins		Species	Score	Length	Source
P00163	Cytochrome b		SACPA		385	UniRef90_P00163
Cytochrome b		YEASX		385
Cytochrome b		YEASK		385
Cytochrome b		YEAS7		385
Cytochrome b		SACMI		385
+15

Protein	Similar proteins		Species	Score	Length	Source
P00163	Cytochrome b	)	HUMAN		380	UniRef50_P00156
Cytochrome b		GORGO		380
Cytochrome b		PANPA		380
Cytochrome b		PANTR		380
Cytochrome b		LIPVE		379
+103492

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	V00696 Genomic DNA Translation: CAA24073.2 Sequence problems. X84042 mRNA Translation: CAA58861.1 KP263414 Genomic DNA Translation: AIZ98890.1 J01473, J01472 Genomic DNA Translation: AAA32151.2 J01475, J01474 Genomic DNA Translation: AAA32152.2
PIRⁱ	A00159 CBBY S78660
RefSeqⁱ	NP_009315.1, NC_001224.1

3D structure databases

Select the link destinations: Protein Data Bank Europe More...PDBeⁱ Protein Data Bank RCSB More...RCSB PDBⁱ Protein Data Bank Japan More...PDBjⁱ Links Updated	PDB entry	Method	Resolution (Å)	Chain	Positions	PDBsum
	1EZV	X-ray	2.30	C	1-385	[»]
	1KB9	X-ray	2.30	C	1-385	[»]
	1KYO	X-ray	2.97	C/N	1-385	[»]
	1P84	X-ray	2.50	C	1-385	[»]
	2IBZ	X-ray	2.30	C	1-385	[»]
	3CX5	X-ray	1.90	C/N	1-385	[»]
	3CXH	X-ray	2.50	C/N	1-385	[»]
	4PD4	X-ray	3.04	C	1-385	[»]
ProteinModelPortalⁱ	P00163
SMRⁱ	P00163
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	34779, 33 interactors
ComplexPortalⁱ	CPX-567 Mitochondrial electron transport complex III
IntActⁱ	P00163, 3 interactors
STRINGⁱ	4932.Q0105

Protein family/group databases

TCDBⁱ	3.D.3.3.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

TCDBⁱ

3.D.3.3.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Proteomic databases

PaxDbⁱ	P00163
PRIDEⁱ	P00163
TopDownProteomicsⁱ	P00163

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblFungiⁱ	Q0105; Q0105; Q0105
GeneIDⁱ	854583
KEGGⁱ	sce:Q0105

Organism-specific databases

EuPathDBⁱ	FungiDB:Q0105
SGDⁱ	S000007270 COB

Phylogenomic databases

GeneTreeⁱ	ENSGT00390000017948
HOGENOMⁱ	HOG000255206
InParanoidⁱ	P00163
KOⁱ	K00412
OMAⁱ	HETGSNN
OrthoDBⁱ	EOG092C2QFL

Enzyme and pathway databases

BioCycⁱ	YEAST:Q0105-MONOMER

BioCycⁱ

YEAST:Q0105-MONOMER

Miscellaneous databases

EvolutionaryTraceⁱ	P00163
Protein Ontology More...PROⁱ	PR:P00163

Family and domain databases

CDDⁱ	cd00290 cytochrome_b_C, 1 hit cd00284 Cytochrome_b_N, 1 hit
Gene3Dⁱ	1.20.810.10, 1 hit
InterProⁱ	View protein in InterPro IPR005798 Cyt_b/b6_C IPR036150 Cyt_b/b6_C_sf IPR005797 Cyt_b/b6_N IPR027387 Cytb/b6-like_sf IPR030689 Cytochrome_b IPR016174 Di-haem_cyt_TM
Pfamⁱ	View protein in Pfam PF00032 Cytochrom_B_C, 1 hit PF00033 Cytochrome_B, 1 hit
PIRSFⁱ	PIRSF038885 COB, 1 hit
SUPFAMⁱ	SSF81342 SSF81342, 1 hit SSF81648 SSF81648, 1 hit
PROSITEⁱ	View protein in PROSITE PS51003 CYTB_CTER, 1 hit PS51002 CYTB_NTER, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	CYB_YEAST
Accessionⁱ	P00163Primary (citable) accession number: P00163 Secondary accession number(s): A0A0A7NYK0 , Q35802, Q35807, Q36301, Q9ZZW9
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	July 21, 1986
	Last sequence update:	April 23, 2003
	Last modified:	July 18, 2018
	This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Fungal Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

PDB cross-references
Index of Protein Data Bank (PDB) cross-references
SIMILARITY comments
Index of protein domains and families
Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

UniProtKB

UniParc

Help

UniRef

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Supporting data

UniProtKB - P00163 (CYB_YEAST)

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Cytochrome b

COB

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

Caution

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Mitochondrion

Mitochondrion

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

3D structure databases

Miscellaneous databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Natural variant

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ