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Protein

Cytochrome b

Gene

Mt-Cyb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.By similarity

Miscellaneous

Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.By similarity

Caution

The full-length protein contains only eight transmembrane helices, not nine as predicted by bioinformatics tools.By similarity

Cofactori

hemeBy similarityNote: Binds 2 heme groups non-covalently.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83Iron 1 (heme b562 axial ligand)PROSITE-ProRule annotation1
Metal bindingi97Iron 2 (heme b566 axial ligand)By similarity1
Metal bindingi182Iron 1 (heme b562 axial ligand)By similarity1
Metal bindingi196Iron 2 (heme b566 axial ligand)By similarity1
Binding sitei201UbiquinoneBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Respiratory chain, Transport
LigandHeme, Iron, Metal-binding, Ubiquinone

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b
Alternative name(s):
Complex III subunit 3
Complex III subunit III
Cytochrome b-c1 complex subunit 3
Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
Gene namesi
Name:Mt-Cyb
Synonyms:Cob, Cytb, mt-Cytb, Mtcyb
Encoded oniMitochondrion
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Mitochondrion

Organism-specific databases

MGIiMGI:102501 mt-Cytb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei33 – 53HelicalBy similarityAdd BLAST21
Transmembranei77 – 98HelicalBy similarityAdd BLAST22
Transmembranei113 – 133HelicalBy similarityAdd BLAST21
Transmembranei178 – 198HelicalBy similarityAdd BLAST21
Transmembranei226 – 246HelicalBy similarityAdd BLAST21
Transmembranei288 – 308HelicalBy similarityAdd BLAST21
Transmembranei320 – 340HelicalBy similarityAdd BLAST21
Transmembranei347 – 367HelicalBy similarityAdd BLAST21

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000612111 – 381Cytochrome bAdd BLAST381

Proteomic databases

PaxDbiP00158
PRIDEiP00158

PTM databases

PhosphoSitePlusiP00158

Expressioni

Gene expression databases

BgeeiENSMUSG00000064370 Expressed in 20 organ(s), highest expression level in heart
ExpressionAtlasiP00158 baseline and differential

Interactioni

Subunit structurei

The cytochrome bc1 complex contains 11 subunits: 3 respiratory subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of UQCRFS1). This cytochrome bc1 complex then forms a dimer.By similarity

Protein-protein interaction databases

BioGridi201544, 1 interactor
ComplexPortaliCPX-563 Mitochondrial respiratory chain complex III
CORUMiP00158
IntActiP00158, 1 interactor
STRINGi10090.ENSMUSP00000081003

Structurei

3D structure databases

ProteinModelPortaliP00158
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4663 Eukaryota
COG1290 LUCA
GeneTreeiENSGT00390000017948
HOGENOMiHOG000255206
HOVERGENiHBG017694
InParanoidiP00158
KOiK00412
OMAiHETGSNN
OrthoDBiEOG091G0DW4
PhylomeDBiP00158
TreeFamiTF353088

Family and domain databases

CDDicd00290 cytochrome_b_C, 1 hit
cd00284 Cytochrome_b_N, 1 hit
Gene3Di1.20.810.10, 1 hit
InterProiView protein in InterPro
IPR005798 Cyt_b/b6_C
IPR036150 Cyt_b/b6_C_sf
IPR005797 Cyt_b/b6_N
IPR027387 Cytb/b6-like_sf
IPR030689 Cytochrome_b
IPR016174 Di-haem_cyt_TM
PfamiView protein in Pfam
PF00032 Cytochrom_B_C, 1 hit
PF00033 Cytochrome_B, 1 hit
PIRSFiPIRSF038885 COB, 1 hit
SUPFAMiSSF81342 SSF81342, 1 hit
SSF81648 SSF81648, 1 hit
PROSITEiView protein in PROSITE
PS51003 CYTB_CTER, 1 hit
PS51002 CYTB_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

P00158-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTNMRKTHPL FKIINHSFID LPAPSNISSW WNFGSLLGVC LMVQIITGLF
60 70 80 90 100
LAMHYTSDTM TAFSSVTHIC RDVNYGWLIR YMHANGASMF FICLFLHVGR
110 120 130 140 150
GLYYGSYTFM ETWNIGVLLL FAVMATAFMG YVLPWGQMSF WGATVITNLL
160 170 180 190 200
SAIPYIGTTL VEWIWGGFSV DKATLTRFFA FHFILPFIIA ALAIVHLLFL
210 220 230 240 250
HETGSNNPTG LNSDADKIPF HPYYTIKDIL GILIMFLILM TLVLFFPDML
260 270 280 290 300
GDPDNYMPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALILSILI
310 320 330 340 350
LALMPFLHTS KQRSLMFRPI TQILYWILVA NLLILTWIGG QPVEHPFIII
360 370 380
GQLASISYFS IILILMPISG IIEDKMLKLY P
Length:381
Mass (Da):43,210
Last modified:July 21, 1986 - v1
Checksum:i6B46D5B1DD8E001A
GO

Sequence cautioni

The sequence CAA24092 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01420 Genomic DNA Translation: AAB48656.1
V00711 Genomic DNA Translation: CAA24092.1 Different initiation.
AY172335 Genomic DNA Translation: AAN85134.1
X57780 mRNA Translation: CAA40926.1
PIRiA00153 CBMS
RefSeqiNP_904340.1, NC_005089.1

Genome annotation databases

EnsembliENSMUST00000082421; ENSMUSP00000081003; ENSMUSG00000064370
GeneIDi17711
KEGGimmu:17711

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J01420 Genomic DNA Translation: AAB48656.1
V00711 Genomic DNA Translation: CAA24092.1 Different initiation.
AY172335 Genomic DNA Translation: AAN85134.1
X57780 mRNA Translation: CAA40926.1
PIRiA00153 CBMS
RefSeqiNP_904340.1, NC_005089.1

3D structure databases

ProteinModelPortaliP00158
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201544, 1 interactor
ComplexPortaliCPX-563 Mitochondrial respiratory chain complex III
CORUMiP00158
IntActiP00158, 1 interactor
STRINGi10090.ENSMUSP00000081003

PTM databases

PhosphoSitePlusiP00158

Proteomic databases

PaxDbiP00158
PRIDEiP00158

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000082421; ENSMUSP00000081003; ENSMUSG00000064370
GeneIDi17711
KEGGimmu:17711

Organism-specific databases

CTDi4519
MGIiMGI:102501 mt-Cytb

Phylogenomic databases

eggNOGiKOG4663 Eukaryota
COG1290 LUCA
GeneTreeiENSGT00390000017948
HOGENOMiHOG000255206
HOVERGENiHBG017694
InParanoidiP00158
KOiK00412
OMAiHETGSNN
OrthoDBiEOG091G0DW4
PhylomeDBiP00158
TreeFamiTF353088

Miscellaneous databases

PROiPR:P00158
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000064370 Expressed in 20 organ(s), highest expression level in heart
ExpressionAtlasiP00158 baseline and differential

Family and domain databases

CDDicd00290 cytochrome_b_C, 1 hit
cd00284 Cytochrome_b_N, 1 hit
Gene3Di1.20.810.10, 1 hit
InterProiView protein in InterPro
IPR005798 Cyt_b/b6_C
IPR036150 Cyt_b/b6_C_sf
IPR005797 Cyt_b/b6_N
IPR027387 Cytb/b6-like_sf
IPR030689 Cytochrome_b
IPR016174 Di-haem_cyt_TM
PfamiView protein in Pfam
PF00032 Cytochrom_B_C, 1 hit
PF00033 Cytochrome_B, 1 hit
PIRSFiPIRSF038885 COB, 1 hit
SUPFAMiSSF81342 SSF81342, 1 hit
SSF81648 SSF81648, 1 hit
PROSITEiView protein in PROSITE
PS51003 CYTB_CTER, 1 hit
PS51002 CYTB_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYB_MOUSE
AccessioniPrimary (citable) accession number: P00158
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 12, 2018
This is version 147 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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