UniProtKB - P00157 (CYB_BOVIN)
Cytochrome b
MT-CYB
Functioni
Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.
1 Publication3 PublicationsMiscellaneous
Caution
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 83 | Iron 1 (heme b562 axial ligand)PROSITE-ProRule annotationCombined sources6 Publications | 1 | |
Metal bindingi | 97 | Iron 2 (heme b566 axial ligand)Combined sources6 Publications | 1 | |
Metal bindingi | 182 | Iron 1 (heme b562 axial ligand)Combined sources6 Publications | 1 | |
Metal bindingi | 196 | Iron 2 (heme b566 axial ligand)Combined sources6 Publications | 1 | |
Binding sitei | 201 | UbiquinoneCombined sources2 Publications | 1 |
GO - Molecular functioni
- heme binding Source: UniProtKB
- metal ion binding Source: UniProtKB-KW
- ubiquinol-cytochrome-c reductase activity Source: UniProtKB
- ubiquinone binding Source: UniProtKB
GO - Biological processi
- mitochondrial electron transport, ubiquinol to cytochrome c Source: UniProtKB
- respiratory electron transport chain Source: UniProtKB
Keywordsi
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | Heme, Iron, Metal-binding, Ubiquinone |
Protein family/group databases
TCDBi | 3.D.3.2.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome bAlternative name(s): Complex III subunit 3 Complex III subunit III Cytochrome b-c1 complex subunit 3 Ubiquinol-cytochrome-c reductase complex cytochrome b subunit |
Gene namesi | Name:MT-CYB Synonyms:COB, CYTB, MTCYB |
Encoded oni | Mitochondrion |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000043550 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 2 Publications1 Publication; Multi-pass membrane protein 6 Publications
Mitochondrion
- integral component of mitochondrial inner membrane Source: Ensembl
- integral component of mitochondrial membrane Source: UniProtKB
- intrinsic component of mitochondrial membrane Source: UniProtKB
- mitochondrial respiratory chain complex III Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 33 – 53 | HelicalCombined sourcesAdd BLAST | 21 | |
Transmembranei | 77 – 98 | HelicalCombined sourcesAdd BLAST | 22 | |
Transmembranei | 113 – 133 | HelicalCombined sourcesAdd BLAST | 21 | |
Transmembranei | 178 – 198 | HelicalCombined sourcesAdd BLAST | 21 | |
Transmembranei | 226 – 246 | HelicalCombined sourcesAdd BLAST | 21 | |
Transmembranei | 288 – 308 | HelicalCombined sourcesAdd BLAST | 21 | |
Transmembranei | 320 – 340 | HelicalCombined sourcesAdd BLAST | 21 | |
Transmembranei | 347 – 367 | HelicalCombined sourcesAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000060686 | 1 – 379 | Cytochrome bAdd BLAST | 379 |
Proteomic databases
PaxDbi | P00157 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSBTAG00000043550, Expressed in pons and 96 other tissues |
Interactioni
Subunit structurei
The cytochrome bc1 complex contains 11 subunits: 3 respiratory subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of UQCRFS1). This cytochrome bc1 complex then forms a dimer.
1 Publication6 PublicationsProtein-protein interaction databases
CORUMi | P00157 |
DIPi | DIP-350N |
IntActi | P00157, 2 interactors |
STRINGi | 9913.ENSBTAP00000053148 |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | P00157 |
SMRi | P00157 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00157 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4663, Eukaryota |
GeneTreei | ENSGT00390000017948 |
HOGENOMi | CLU_031114_3_0_1 |
InParanoidi | P00157 |
OMAi | RFFAFHF |
OrthoDBi | 1125966at2759 |
TreeFami | TF353088 |
Family and domain databases
CDDi | cd00290, cytochrome_b_C, 1 hit cd00284, Cytochrome_b_N, 1 hit |
Gene3Di | 1.20.810.10, 1 hit |
InterProi | View protein in InterPro IPR005798, Cyt_b/b6_C IPR036150, Cyt_b/b6_C_sf IPR005797, Cyt_b/b6_N IPR027387, Cytb/b6-like_sf IPR030689, Cytochrome_b IPR016174, Di-haem_cyt_TM |
Pfami | View protein in Pfam PF00032, Cytochrom_B_C, 1 hit PF00033, Cytochrome_B, 1 hit |
PIRSFi | PIRSF038885, COB, 1 hit |
SUPFAMi | SSF81342, SSF81342, 1 hit SSF81648, SSF81648, 1 hit |
PROSITEi | View protein in PROSITE PS51003, CYTB_CTER, 1 hit PS51002, CYTB_NTER, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MTNIRKSHPL MKIVNNAFID LPAPSNISSW WNFGSLLGIC LILQILTGLF
60 70 80 90 100
LAMHYTSDTT TAFSSVTHIC RDVNYGWIIR YMHANGASMF FICLYMHVGR
110 120 130 140 150
GLYYGSYTFL ETWNIGVILL LTVMATAFMG YVLPWGQMSF WGATVITNLL
160 170 180 190 200
SAIPYIGTNL VEWIWGGFSV DKATLTRFFA FHFILPFIIM AIAMVHLLFL
210 220 230 240 250
HETGSNNPTG ISSDVDKIPF HPYYTIKDIL GALLLILALM LLVLFAPDLL
260 270 280 290 300
GDPDNYTPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALAFSILI
310 320 330 340 350
LALIPLLHTS KQRSMMFRPL SQCLFWALVA DLLTLTWIGG QPVEHPYITI
360 370
GQLASVLYFL LILVLMPTAG TIENKLLKW
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural varianti | 90 | F → V in strain: 65, 66 and D. | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00654 Genomic DNA Translation: CAA24007.1 AF490528 Genomic DNA Translation: AAM08329.1 AF490529 Genomic DNA Translation: AAM08342.1 AF493541 Genomic DNA Translation: AAM12801.1 AF493542 Genomic DNA Translation: AAM12814.1 |
PIRi | A00152, CBBO |
RefSeqi | YP_209217.1, NC_006853.1 |
Genome annotation databases
Ensembli | ENSBTAT00000060567; ENSBTAP00000053148; ENSBTAG00000043550 |
GeneIDi | 3283889 |
KEGGi | bta:3283889 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | V00654 Genomic DNA Translation: CAA24007.1 AF490528 Genomic DNA Translation: AAM08329.1 AF490529 Genomic DNA Translation: AAM08342.1 AF493541 Genomic DNA Translation: AAM12801.1 AF493542 Genomic DNA Translation: AAM12814.1 |
PIRi | A00152, CBBO |
RefSeqi | YP_209217.1, NC_006853.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BE3 | X-ray | 3.00 | C | 1-379 | [»] | |
1BGY | X-ray | 3.00 | C/O | 1-379 | [»] | |
1L0L | X-ray | 2.35 | C | 1-379 | [»] | |
1L0N | X-ray | 2.60 | C | 1-379 | [»] | |
1NTK | X-ray | 2.60 | C | 1-379 | [»] | |
1NTM | X-ray | 2.40 | C | 1-379 | [»] | |
1NTZ | X-ray | 2.60 | C | 1-379 | [»] | |
1NU1 | X-ray | 3.20 | C | 1-379 | [»] | |
1PP9 | X-ray | 2.10 | C/P | 1-379 | [»] | |
1PPJ | X-ray | 2.10 | C/P | 1-379 | [»] | |
1QCR | X-ray | 2.70 | C | 2-379 | [»] | |
1SQB | X-ray | 2.69 | C | 1-379 | [»] | |
1SQP | X-ray | 2.70 | C | 1-379 | [»] | |
1SQQ | X-ray | 3.00 | C | 1-379 | [»] | |
1SQV | X-ray | 2.85 | C | 1-379 | [»] | |
1SQX | X-ray | 2.60 | C | 1-379 | [»] | |
2A06 | X-ray | 2.10 | C/P | 1-379 | [»] | |
2FYU | X-ray | 2.26 | C | 1-379 | [»] | |
2YBB | electron microscopy | 19.00 | C/c | 1-379 | [»] | |
4D6T | X-ray | 3.57 | C/P | 1-379 | [»] | |
4D6U | X-ray | 4.09 | C/P | 1-379 | [»] | |
5GPN | electron microscopy | 5.40 | C/O | 1-379 | [»] | |
5KLV | X-ray | 2.65 | C | 1-379 | [»] | |
5LUF | electron microscopy | 9.10 | b/o | 1-379 | [»] | |
5NMI | X-ray | 3.50 | C/P | 8-379 | [»] | |
5OKD | X-ray | 3.10 | C | 1-379 | [»] | |
6FO0 | electron microscopy | 4.10 | C/P | 1-379 | [»] | |
6FO2 | electron microscopy | 4.40 | C/P | 1-379 | [»] | |
6FO6 | electron microscopy | 4.10 | C/P | 1-379 | [»] | |
6HAW | X-ray | 3.45 | C | 2-379 | [»] | |
6NHG | X-ray | 2.80 | C | 1-379 | [»] | |
6XVF | X-ray | 3.50 | C | 2-379 | [»] | |
6ZFS | X-ray | 3.50 | C | 2-379 | [»] | |
6ZFT | X-ray | 3.30 | C | 2-379 | [»] | |
6ZFU | X-ray | 3.50 | C | 2-379 | [»] | |
AlphaFoldDBi | P00157 | |||||
SMRi | P00157 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
CORUMi | P00157 |
DIPi | DIP-350N |
IntActi | P00157, 2 interactors |
STRINGi | 9913.ENSBTAP00000053148 |
Protein family/group databases
TCDBi | 3.D.3.2.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Proteomic databases
PaxDbi | P00157 |
Genome annotation databases
Ensembli | ENSBTAT00000060567; ENSBTAP00000053148; ENSBTAG00000043550 |
GeneIDi | 3283889 |
KEGGi | bta:3283889 |
Organism-specific databases
CTDi | 4519 |
VEuPathDBi | HostDB:ENSBTAG00000043550 |
Phylogenomic databases
eggNOGi | KOG4663, Eukaryota |
GeneTreei | ENSGT00390000017948 |
HOGENOMi | CLU_031114_3_0_1 |
InParanoidi | P00157 |
OMAi | RFFAFHF |
OrthoDBi | 1125966at2759 |
TreeFami | TF353088 |
Miscellaneous databases
EvolutionaryTracei | P00157 |
PROi | PR:P00157 |
Gene expression databases
Bgeei | ENSBTAG00000043550, Expressed in pons and 96 other tissues |
Family and domain databases
CDDi | cd00290, cytochrome_b_C, 1 hit cd00284, Cytochrome_b_N, 1 hit |
Gene3Di | 1.20.810.10, 1 hit |
InterProi | View protein in InterPro IPR005798, Cyt_b/b6_C IPR036150, Cyt_b/b6_C_sf IPR005797, Cyt_b/b6_N IPR027387, Cytb/b6-like_sf IPR030689, Cytochrome_b IPR016174, Di-haem_cyt_TM |
Pfami | View protein in Pfam PF00032, Cytochrom_B_C, 1 hit PF00033, Cytochrome_B, 1 hit |
PIRSFi | PIRSF038885, COB, 1 hit |
SUPFAMi | SSF81342, SSF81342, 1 hit SSF81648, SSF81648, 1 hit |
PROSITEi | View protein in PROSITE PS51003, CYTB_CTER, 1 hit PS51002, CYTB_NTER, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CYB_BOVIN | |
Accessioni | P00157Primary (citable) accession number: P00157 Secondary accession number(s): Q8SE06 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | July 21, 1986 | |
Last modified: | May 25, 2022 | |
This is version 168 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families