Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Cytochrome b

Gene

MT-CYB

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex) that is part of the mitochondrial respiratory chain. The b-c1 complex mediates electron transfer from ubiquinol to cytochrome c. Contributes to the generation of a proton gradient across the mitochondrial membrane that is then used for ATP synthesis.1 Publication3 Publications

Miscellaneous

Heme 1 (or BL or b562) is low-potential and absorbs at about 562 nm, and heme 2 (or BH or b566) is high-potential and absorbs at about 566 nm.

Caution

The full-length protein contains only eight transmembrane helices, not nine as predicted by bioinformatics tools.6 Publications

Cofactori

heme6 PublicationsNote: Binds 2 heme groups non-covalently.6 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi83Iron 1 (heme b562 axial ligand)PROSITE-ProRule annotationCombined sources6 Publications1
Metal bindingi97Iron 2 (heme b566 axial ligand)Combined sources6 Publications1
Metal bindingi182Iron 1 (heme b562 axial ligand)Combined sources6 Publications1
Metal bindingi196Iron 2 (heme b566 axial ligand)Combined sources6 Publications1
Binding sitei201UbiquinoneCombined sources2 Publications1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processElectron transport, Respiratory chain, Transport
LigandHeme, Iron, Metal-binding, Ubiquinone

Enzyme and pathway databases

ReactomeiR-BTA-611105 Respiratory electron transport

Protein family/group databases

TCDBi3.D.3.2.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome b
Alternative name(s):
Complex III subunit 3
Complex III subunit III
Cytochrome b-c1 complex subunit 3
Ubiquinol-cytochrome-c reductase complex cytochrome b subunit
Gene namesi
Name:MT-CYB
Synonyms:COB, CYTB, MTCYB
Encoded oniMitochondrion
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Mitochondrion

Organism-specific databases

VGNCiVGNC:55738 MT-CYB

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei33 – 53HelicalCombined sourcesAdd BLAST21
Transmembranei77 – 98HelicalCombined sourcesAdd BLAST22
Transmembranei113 – 133HelicalCombined sourcesAdd BLAST21
Transmembranei178 – 198HelicalCombined sourcesAdd BLAST21
Transmembranei226 – 246HelicalCombined sourcesAdd BLAST21
Transmembranei288 – 308HelicalCombined sourcesAdd BLAST21
Transmembranei320 – 340HelicalCombined sourcesAdd BLAST21
Transmembranei347 – 367HelicalCombined sourcesAdd BLAST21

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000606861 – 379Cytochrome bAdd BLAST379

Proteomic databases

PaxDbiP00157
PRIDEiP00157

Expressioni

Tissue specificityi

Detected in heart (at protein level).3 Publications

Gene expression databases

BgeeiENSBTAG00000043550 Expressed in 9 organ(s), highest expression level in prefrontal cortex
ExpressionAtlasiP00157 baseline

Interactioni

Subunit structurei

The cytochrome bc1 complex contains 11 subunits: 3 respiratory subunits (MT-CYB, CYC1 and UQCRFS1), 2 core proteins (UQCRC1 and UQCRC2) and 6 low-molecular weight proteins (UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and a cleavage product of UQCRFS1). This cytochrome bc1 complex then forms a dimer.1 Publication6 Publications

Protein-protein interaction databases

CORUMiP00157
DIPiDIP-350N
IntActiP00157, 2 interactors
STRINGi9913.ENSBTAP00000053148

Structurei

Secondary structure

1379
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliP00157
SMRiP00157
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00157

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome b family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG4663 Eukaryota
COG1290 LUCA
GeneTreeiENSGT00390000017948
HOGENOMiHOG000255206
HOVERGENiHBG017694
InParanoidiP00157
KOiK00412
OMAiHETGSNN
OrthoDBiEOG091G0DW4
TreeFamiTF353088

Family and domain databases

CDDicd00290 cytochrome_b_C, 1 hit
cd00284 Cytochrome_b_N, 1 hit
Gene3Di1.20.810.10, 1 hit
InterProiView protein in InterPro
IPR005798 Cyt_b/b6_C
IPR036150 Cyt_b/b6_C_sf
IPR005797 Cyt_b/b6_N
IPR027387 Cytb/b6-like_sf
IPR030689 Cytochrome_b
IPR016174 Di-haem_cyt_TM
PfamiView protein in Pfam
PF00032 Cytochrom_B_C, 1 hit
PF00033 Cytochrome_B, 1 hit
PIRSFiPIRSF038885 COB, 1 hit
SUPFAMiSSF81342 SSF81342, 1 hit
SSF81648 SSF81648, 1 hit
PROSITEiView protein in PROSITE
PS51003 CYTB_CTER, 1 hit
PS51002 CYTB_NTER, 1 hit

Sequencei

Sequence statusi: Complete.

P00157-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTNIRKSHPL MKIVNNAFID LPAPSNISSW WNFGSLLGIC LILQILTGLF
60 70 80 90 100
LAMHYTSDTT TAFSSVTHIC RDVNYGWIIR YMHANGASMF FICLYMHVGR
110 120 130 140 150
GLYYGSYTFL ETWNIGVILL LTVMATAFMG YVLPWGQMSF WGATVITNLL
160 170 180 190 200
SAIPYIGTNL VEWIWGGFSV DKATLTRFFA FHFILPFIIM AIAMVHLLFL
210 220 230 240 250
HETGSNNPTG ISSDVDKIPF HPYYTIKDIL GALLLILALM LLVLFAPDLL
260 270 280 290 300
GDPDNYTPAN PLNTPPHIKP EWYFLFAYAI LRSIPNKLGG VLALAFSILI
310 320 330 340 350
LALIPLLHTS KQRSMMFRPL SQCLFWALVA DLLTLTWIGG QPVEHPYITI
360 370
GQLASVLYFL LILVLMPTAG TIENKLLKW
Length:379
Mass (Da):42,591
Last modified:July 21, 1986 - v1
Checksum:i90160B99E031E016
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti90F → V in strain: 65, 66 and D. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA Translation: CAA24007.1
AF490528 Genomic DNA Translation: AAM08329.1
AF490529 Genomic DNA Translation: AAM08342.1
AF493541 Genomic DNA Translation: AAM12801.1
AF493542 Genomic DNA Translation: AAM12814.1
PIRiA00152 CBBO
RefSeqiYP_209217.1, NC_006853.1

Genome annotation databases

EnsembliENSBTAT00000060567; ENSBTAP00000053148; ENSBTAG00000043550
GeneIDi3283889
KEGGibta:3283889

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
V00654 Genomic DNA Translation: CAA24007.1
AF490528 Genomic DNA Translation: AAM08329.1
AF490529 Genomic DNA Translation: AAM08342.1
AF493541 Genomic DNA Translation: AAM12801.1
AF493542 Genomic DNA Translation: AAM12814.1
PIRiA00152 CBBO
RefSeqiYP_209217.1, NC_006853.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1BE3X-ray3.00C1-379[»]
1BGYX-ray3.00C/O1-379[»]
1L0LX-ray2.35C1-379[»]
1L0NX-ray2.60C1-379[»]
1NTKX-ray2.60C1-379[»]
1NTMX-ray2.40C1-379[»]
1NTZX-ray2.60C1-379[»]
1NU1X-ray3.20C1-379[»]
1PP9X-ray2.10C/P1-379[»]
1PPJX-ray2.10C/P1-379[»]
1QCRX-ray2.70C2-379[»]
1SQBX-ray2.69C1-379[»]
1SQPX-ray2.70C1-379[»]
1SQQX-ray3.00C1-379[»]
1SQVX-ray2.85C1-379[»]
1SQXX-ray2.60C1-379[»]
2A06X-ray2.10C/P1-379[»]
2FYUX-ray2.26C1-379[»]
2YBBelectron microscopy19.00C/c1-379[»]
4D6TX-ray3.57C/P1-379[»]
4D6UX-ray4.09C/P1-379[»]
5GPNelectron microscopy5.40C/O1-379[»]
5KLVX-ray2.65C1-379[»]
5LUFelectron microscopy9.10b/o1-379[»]
5NMIX-ray3.50C/P8-379[»]
5OKDX-ray3.10C1-379[»]
6FO0electron microscopy4.10C/P1-379[»]
6FO2electron microscopy4.40C/P1-379[»]
6FO6electron microscopy4.10C/P1-379[»]
ProteinModelPortaliP00157
SMRiP00157
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

CORUMiP00157
DIPiDIP-350N
IntActiP00157, 2 interactors
STRINGi9913.ENSBTAP00000053148

Protein family/group databases

TCDBi3.D.3.2.1 the proton-translocating quinol:cytochrome c reductase (qcr) superfamily

Proteomic databases

PaxDbiP00157
PRIDEiP00157

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000060567; ENSBTAP00000053148; ENSBTAG00000043550
GeneIDi3283889
KEGGibta:3283889

Organism-specific databases

CTDi4519
VGNCiVGNC:55738 MT-CYB

Phylogenomic databases

eggNOGiKOG4663 Eukaryota
COG1290 LUCA
GeneTreeiENSGT00390000017948
HOGENOMiHOG000255206
HOVERGENiHBG017694
InParanoidiP00157
KOiK00412
OMAiHETGSNN
OrthoDBiEOG091G0DW4
TreeFamiTF353088

Enzyme and pathway databases

ReactomeiR-BTA-611105 Respiratory electron transport

Miscellaneous databases

EvolutionaryTraceiP00157
PROiPR:P00157

Gene expression databases

BgeeiENSBTAG00000043550 Expressed in 9 organ(s), highest expression level in prefrontal cortex
ExpressionAtlasiP00157 baseline

Family and domain databases

CDDicd00290 cytochrome_b_C, 1 hit
cd00284 Cytochrome_b_N, 1 hit
Gene3Di1.20.810.10, 1 hit
InterProiView protein in InterPro
IPR005798 Cyt_b/b6_C
IPR036150 Cyt_b/b6_C_sf
IPR005797 Cyt_b/b6_N
IPR027387 Cytb/b6-like_sf
IPR030689 Cytochrome_b
IPR016174 Di-haem_cyt_TM
PfamiView protein in Pfam
PF00032 Cytochrom_B_C, 1 hit
PF00033 Cytochrome_B, 1 hit
PIRSFiPIRSF038885 COB, 1 hit
SUPFAMiSSF81342 SSF81342, 1 hit
SSF81648 SSF81648, 1 hit
PROSITEiView protein in PROSITE
PS51003 CYTB_CTER, 1 hit
PS51002 CYTB_NTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYB_BOVIN
AccessioniPrimary (citable) accession number: P00157
Secondary accession number(s): Q8SE06
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: July 21, 1986
Last modified: September 12, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again