UniProtKB - P00127 (QCR6_YEAST)
Cytochrome b-c1 complex subunit 6, mitochondrial
QCR6
Functioni
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c.
1 PublicationMiscellaneous
GO - Biological processi
- aerobic respiration Source: SGD
- cellular respiration Source: ComplexPortal
- mitochondrial electron transport, ubiquinol to cytochrome c Source: ComplexPortal
- proton transmembrane transport Source: GOC
Keywordsi
Biological process | Electron transport, Respiratory chain, Transport |
Enzyme and pathway databases
Reactomei | R-SCE-611105, Respiratory electron transport |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome b-c1 complex subunit 6, mitochondrialAlternative name(s): Complex III subunit 6 Complex III subunit VI Cytochrome c1 non-heme 17 kDa protein Mitochondrial hinge protein Ubiquinol-cytochrome c oxidoreductase subunit 6 Ubiquinol-cytochrome c reductase 17 kDa protein |
Gene namesi | Name:QCR6 Synonyms:UCR6 Ordered Locus Names:YFR033C |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000001929, QCR6 |
VEuPathDBi | FungiDB:YFR033C |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane 3 Publications; Peripheral membrane protein 2 Publications; Intermembrane side 2 Publications
Mitochondrion
- mitochondrial inner membrane Source: ComplexPortal
- mitochondrial intermembrane space Source: SGD
- mitochondrial respiratory chain complex III Source: SGD
- mitochondrion Source: SGD
Nucleus
- nuclear periphery Source: SGD
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193542 | 1 – 147 | Cytochrome b-c1 complex subunit 6, mitochondrialAdd BLAST | 147 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 101 ↔ 123 | 6 Publications |
Keywords - PTMi
Disulfide bondProteomic databases
MaxQBi | P00127 |
PaxDbi | P00127 |
PRIDEi | P00127 |
PTM databases
CarbonylDBi | P00127 |
Interactioni
Subunit structurei
Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 10 subunits. The complex is composed of 3 respiratory subunits cytochrome b (COB), cytochrome c1 (CYT1) and Rieske protein (RIP1), 2 core protein subunits COR1 and QCR2, and 5 low-molecular weight protein subunits QCR6, QCR7, QCR8, QCR9 and QCR10 (PubMed:10873857, PubMed:11880631, PubMed:18390544, PubMed:30598554). The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with a monomer or a dimer of cytochrome c oxidase (complex IV, CIV), resulting in 2 different assemblies (supercomplexes III2IV and III2IV2) (PubMed:10775262, PubMed:10764779, PubMed:30598556, PubMed:30598554). QCR6 interacts with COX5A at the CIII-CIV interface (PubMed:30598554).
7 PublicationsProtein-protein interaction databases
BioGRIDi | 31190, 112 interactors |
ComplexPortali | CPX-567, Mitochondrial respiratory chain complex III |
DIPi | DIP-1621N |
IntActi | P00127, 5 interactors |
MINTi | P00127 |
STRINGi | 4932.YFR033C |
Miscellaneous databases
RNActi | P00127, protein |
Structurei
Secondary structure
3D structure databases
SMRi | P00127 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00127 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 25 – 89 | DisorderedSequence analysisAdd BLAST | 65 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 26 – 40 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 41 – 77 | Acidic residuesSequence analysisAdd BLAST | 37 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4763, Eukaryota |
GeneTreei | ENSGT00390000003860 |
HOGENOMi | CLU_115913_0_1_1 |
InParanoidi | P00127 |
OMAi | CKNSPQC |
Family and domain databases
DisProti | DP00853 |
Gene3Di | 1.10.287.20, 1 hit |
InterProi | View protein in InterPro IPR023184, Ubol_cytC_Rdtase_hinge_dom IPR036811, Ubol_cytC_Rdtase_hinge_dom_sf |
Pfami | View protein in Pfam PF02320, UCR_hinge, 1 hit |
SUPFAMi | SSF81531, SSF81531, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MGMLELVGEY WEQLKITVVP VVAAAEDDDN EQHEEKAAEG EEKEEENGDE
60 70 80 90 100
DEDEDEDEDD DDDDDEDEEE EEEVTDQLED LREHFKNTEE GKALVHHYEE
110 120 130 140
CAERVKIQQQ QPGYADLEHK EDCVEEFFHL QHYLDTATAP RLFDKLK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 | G → D in CAA25220 (PubMed:6329732).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00551 Genomic DNA Translation: CAA25220.1 D50617 Genomic DNA Translation: BAA09272.1 BK006940 Genomic DNA Translation: DAA12476.1 |
PIRi | S56288, RDBYUC |
RefSeqi | NP_116691.3, NM_001179998.3 |
Genome annotation databases
EnsemblFungii | YFR033C_mRNA; YFR033C; YFR033C |
GeneIDi | 850593 |
KEGGi | sce:YFR033C |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | X00551 Genomic DNA Translation: CAA25220.1 D50617 Genomic DNA Translation: BAA09272.1 BK006940 Genomic DNA Translation: DAA12476.1 |
PIRi | S56288, RDBYUC |
RefSeqi | NP_116691.3, NM_001179998.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1EZV | X-ray | 2.30 | H | 74-147 | [»] | |
1KB9 | X-ray | 2.30 | F | 74-147 | [»] | |
1KYO | X-ray | 2.97 | F/Q | 75-147 | [»] | |
1P84 | X-ray | 2.50 | F | 74-147 | [»] | |
2IBZ | X-ray | 2.30 | H | 74-147 | [»] | |
3CX5 | X-ray | 1.90 | F/Q | 2-147 | [»] | |
3CXH | X-ray | 2.50 | F/Q | 2-147 | [»] | |
4PD4 | X-ray | 3.04 | F | 74-147 | [»] | |
6GIQ | electron microscopy | 3.23 | F/Q | 1-147 | [»] | |
6HU9 | electron microscopy | 3.35 | F/Q | 1-147 | [»] | |
6T0B | electron microscopy | 2.80 | F/Q | 1-147 | [»] | |
6T15 | electron microscopy | 3.29 | F/Q | 1-147 | [»] | |
SMRi | P00127 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 31190, 112 interactors |
ComplexPortali | CPX-567, Mitochondrial respiratory chain complex III |
DIPi | DIP-1621N |
IntActi | P00127, 5 interactors |
MINTi | P00127 |
STRINGi | 4932.YFR033C |
PTM databases
CarbonylDBi | P00127 |
Proteomic databases
MaxQBi | P00127 |
PaxDbi | P00127 |
PRIDEi | P00127 |
Genome annotation databases
EnsemblFungii | YFR033C_mRNA; YFR033C; YFR033C |
GeneIDi | 850593 |
KEGGi | sce:YFR033C |
Organism-specific databases
SGDi | S000001929, QCR6 |
VEuPathDBi | FungiDB:YFR033C |
Phylogenomic databases
eggNOGi | KOG4763, Eukaryota |
GeneTreei | ENSGT00390000003860 |
HOGENOMi | CLU_115913_0_1_1 |
InParanoidi | P00127 |
OMAi | CKNSPQC |
Enzyme and pathway databases
Reactomei | R-SCE-611105, Respiratory electron transport |
Miscellaneous databases
EvolutionaryTracei | P00127 |
PROi | PR:P00127 |
RNActi | P00127, protein |
Family and domain databases
DisProti | DP00853 |
Gene3Di | 1.10.287.20, 1 hit |
InterProi | View protein in InterPro IPR023184, Ubol_cytC_Rdtase_hinge_dom IPR036811, Ubol_cytC_Rdtase_hinge_dom_sf |
Pfami | View protein in Pfam PF02320, UCR_hinge, 1 hit |
SUPFAMi | SSF81531, SSF81531, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | QCR6_YEAST | |
Accessioni | P00127Primary (citable) accession number: P00127 Secondary accession number(s): D6VTR6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | November 1, 1995 | |
Last modified: | February 23, 2022 | |
This is version 193 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome VI
Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families