UniProtKB - P00125 (CY1_BOVIN)
Cytochrome c1, heme protein, mitochondrial
CYC1
Functioni
Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c. Cytochrome c1 is a catalytic core subunit containing a c-type heme. It transfers electrons from the [2Fe-2S] iron-sulfur cluster of the Rieske protein to cytochrome c.
By similarityCatalytic activityi
- a quinol + 2 Fe(III)-[cytochrome c](out) = a quinone + 2 Fe(II)-[cytochrome c](out) + 2 H+(out)By similarityEC:7.1.1.8By similarity
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 121 | Heme; covalentCombined sources1 Publication | 1 | |
Binding sitei | 124 | Heme; covalentCombined sources1 Publication | 1 | |
Metal bindingi | 125 | Iron (heme axial ligand); via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 244 | Iron (heme axial ligand)Combined sources1 Publication | 1 |
GO - Molecular functioni
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
- ubiquinol-cytochrome-c reductase activity Source: UniProtKB-EC
GO - Biological processi
- mitochondrial electron transport, ubiquinol to cytochrome c Source: GO_Central
Keywordsi
Molecular function | Translocase |
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | Heme, Iron, Metal-binding |
Protein family/group databases
TCDBi | 3.D.3.2.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome c1, heme protein, mitochondrial (EC:7.1.1.8)Alternative name(s): Complex III subunit 4 Complex III subunit IV Cytochrome b-c1 complex subunit 4 Ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit Short name: Cytochrome c-1 |
Gene namesi | Name:CYC1 |
Organismi | Bos taurus (Bovine) |
Taxonomic identifieri | 9913 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Artiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos |
Proteomesi |
|
Organism-specific databases
VEuPathDBi | HostDB:ENSBTAG00000012232 |
VGNCi | VGNC:50266, CYC1 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Single-pass membrane protein By similarity
Mitochondrion
- mitochondrial inner membrane Source: AgBase
- mitochondrial respiratory chain complex III Source: GO_Central
- mitochondrion Source: AgBase
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 85 – 281 | Mitochondrial intermembrane1 PublicationAdd BLAST | 197 | |
Transmembranei | 282 – 315 | Helical1 PublicationAdd BLAST | 34 | |
Topological domaini | 316 – 325 | Mitochondrial matrix1 Publication | 10 |
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 84 | Mitochondrion1 PublicationAdd BLAST | 84 | |
ChainiPRO_0000108416 | 85 – 325 | Cytochrome c1, heme protein, mitochondrialAdd BLAST | 241 |
Proteomic databases
PaxDbi | P00125 |
PeptideAtlasi | P00125 |
PRIDEi | P00125 |
Expressioni
Gene expression databases
Bgeei | ENSBTAG00000012232, Expressed in heart and 97 other tissues |
Interactioni
Subunit structurei
Component of the ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII), a multisubunit enzyme composed of 11 subunits. The complex is composed of 3 respiratory subunits cytochrome b, cytochrome c1 and Rieske protein UQCRFS1, 2 core protein subunits UQCRC1/QCR1 and UQCRC2/QCR2, and 6 low-molecular weight protein subunits UQCRH/QCR6, UQCRB/QCR7, UQCRQ/QCR8, UQCR10/QCR9, UQCR11/QCR10 and subunit 9, the cleavage product of Rieske protein UQCRFS1 (PubMed:9651245). The complex exists as an obligatory dimer and forms supercomplexes (SCs) in the inner mitochondrial membrane with NADH-ubiquinone oxidoreductase (complex I, CI) and cytochrome c oxidase (complex IV, CIV), resulting in different assemblies (supercomplex SCI1III2IV1 and megacomplex MCI2III2IV2) (PubMed:27830641).
2 PublicationsProtein-protein interaction databases
CORUMi | P00125 |
DIPi | DIP-38976N |
IntActi | P00125, 10 interactors |
STRINGi | 9913.ENSBTAP00000016224 |
Structurei
Secondary structure
3D structure databases
SMRi | P00125 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00125 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 108 – 209 | Cytochrome cPROSITE-ProRule annotationAdd BLAST | 102 |
Sequence similaritiesi
Keywords - Domaini
Transit peptide, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG3052, Eukaryota |
GeneTreei | ENSGT00390000012445 |
HOGENOMi | CLU_040334_1_0_1 |
InParanoidi | P00125 |
OMAi | VWVKKFK |
OrthoDBi | 923710at2759 |
TreeFami | TF314799 |
Family and domain databases
Gene3Di | 1.10.760.10, 1 hit |
InterProi | View protein in InterPro IPR009056, Cyt_c-like_dom IPR036909, Cyt_c-like_dom_sf IPR002326, Cyt_c1 IPR021157, Cyt_c1_TM_anchor_C |
PANTHERi | PTHR10266, PTHR10266, 1 hit |
Pfami | View protein in Pfam PF02167, Cytochrom_C1, 1 hit |
PRINTSi | PR00603, CYTOCHROMEC1 |
SUPFAMi | SSF46626, SSF46626, 1 hit SSF81496, SSF81496, 1 hit |
PROSITEi | View protein in PROSITE PS51007, CYTC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MAAAAATLRG AMVGPRGAGL PGARARGLLC GARPGQLPLR TPQAVSLSSK
60 70 80 90 100
SGLSRGRKVI LSALGMLAAG GAGLAVALHS AVSASDLELH PPSYPWSHRG
110 120 130 140 150
LLSSLDHTSI RRGFQVYKQV CSSCHSMDYV AYRHLVGVCY TEDEAKALAE
160 170 180 190 200
EVEVQDGPNE DGEMFMRPGK LSDYFPKPYP NPEAARAANN GALPPDLSYI
210 220 230 240 250
VRARHGGEDY VFSLLTGYCE PPTGVSLREG LYFNPYFPGQ AIGMAPPIYN
260 270 280 290 300
EVLEFDDGTP ATMSQVAKDV CTFLRWAAEP EHDHRKRMGL KMLLMMGLLL
310 320
PLVYAMKRHK WSVLKSRKLA YRPPK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC109917 mRNA Translation: AAI09918.1 U97172 Genomic DNA Translation: AAB57834.1 |
PIRi | A00118, CCBO1 |
RefSeqi | NP_001033179.1, NM_001038090.2 |
Genome annotation databases
Ensembli | ENSBTAT00000016224; ENSBTAP00000016224; ENSBTAG00000012232 |
GeneIDi | 512500 |
KEGGi | bta:512500 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BC109917 mRNA Translation: AAI09918.1 U97172 Genomic DNA Translation: AAB57834.1 |
PIRi | A00118, CCBO1 |
RefSeqi | NP_001033179.1, NM_001038090.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1BCC | X-ray | 3.16 | D | 85-325 | [»] | |
1BE3 | X-ray | 3.00 | D | 85-325 | [»] | |
1BGY | X-ray | 3.00 | D/P | 85-325 | [»] | |
1L0L | X-ray | 2.35 | D | 85-325 | [»] | |
1L0N | X-ray | 2.60 | D | 85-325 | [»] | |
1NTK | X-ray | 2.60 | D | 85-325 | [»] | |
1NTM | X-ray | 2.40 | D | 85-325 | [»] | |
1NTZ | X-ray | 2.60 | D | 85-325 | [»] | |
1NU1 | X-ray | 3.20 | D | 85-325 | [»] | |
1PP9 | X-ray | 2.10 | D/Q | 85-325 | [»] | |
1PPJ | X-ray | 2.10 | D/Q | 85-325 | [»] | |
1QCR | X-ray | 2.70 | D | 251-325 | [»] | |
1SQB | X-ray | 2.69 | D | 85-325 | [»] | |
1SQP | X-ray | 2.70 | D | 85-325 | [»] | |
1SQQ | X-ray | 3.00 | D | 85-325 | [»] | |
1SQV | X-ray | 2.85 | D | 85-325 | [»] | |
1SQX | X-ray | 2.60 | D | 85-325 | [»] | |
2A06 | X-ray | 2.10 | D/Q | 85-325 | [»] | |
2BCC | X-ray | 3.50 | D | 85-325 | [»] | |
2FYU | X-ray | 2.26 | D | 85-325 | [»] | |
2YBB | electron microscopy | 19.00 | D/d | 85-325 | [»] | |
3BCC | X-ray | 3.70 | D | 85-325 | [»] | |
4D6T | X-ray | 3.57 | D/Q | 61-325 | [»] | |
4D6U | X-ray | 4.09 | D/Q | 1-325 | [»] | |
5GPN | electron microscopy | 5.40 | D/P | 85-325 | [»] | |
5KLV | X-ray | 2.65 | D | 85-325 | [»] | |
5LUF | electron microscopy | 9.10 | d/p | 85-325 | [»] | |
5NMI | X-ray | 3.50 | D/Q | 86-325 | [»] | |
5OKD | X-ray | 3.10 | D | 1-325 | [»] | |
6FO0 | electron microscopy | 4.10 | D/Q | 1-325 | [»] | |
6FO2 | electron microscopy | 4.40 | D/Q | 1-325 | [»] | |
6FO6 | electron microscopy | 4.10 | D/Q | 1-325 | [»] | |
6HAW | X-ray | 3.45 | D | 86-324 | [»] | |
6NHG | X-ray | 2.80 | D | 85-325 | [»] | |
6XVF | X-ray | 3.50 | D | 86-324 | [»] | |
6ZFS | X-ray | 3.50 | D | 86-324 | [»] | |
6ZFT | X-ray | 3.30 | D | 86-324 | [»] | |
6ZFU | X-ray | 3.50 | D | 86-324 | [»] | |
SMRi | P00125 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
CORUMi | P00125 |
DIPi | DIP-38976N |
IntActi | P00125, 10 interactors |
STRINGi | 9913.ENSBTAP00000016224 |
Protein family/group databases
TCDBi | 3.D.3.2.1, the proton-translocating quinol:cytochrome c reductase (qcr) superfamily |
Proteomic databases
PaxDbi | P00125 |
PeptideAtlasi | P00125 |
PRIDEi | P00125 |
Genome annotation databases
Ensembli | ENSBTAT00000016224; ENSBTAP00000016224; ENSBTAG00000012232 |
GeneIDi | 512500 |
KEGGi | bta:512500 |
Organism-specific databases
CTDi | 1537 |
VEuPathDBi | HostDB:ENSBTAG00000012232 |
VGNCi | VGNC:50266, CYC1 |
Phylogenomic databases
eggNOGi | KOG3052, Eukaryota |
GeneTreei | ENSGT00390000012445 |
HOGENOMi | CLU_040334_1_0_1 |
InParanoidi | P00125 |
OMAi | VWVKKFK |
OrthoDBi | 923710at2759 |
TreeFami | TF314799 |
Miscellaneous databases
EvolutionaryTracei | P00125 |
Gene expression databases
Bgeei | ENSBTAG00000012232, Expressed in heart and 97 other tissues |
Family and domain databases
Gene3Di | 1.10.760.10, 1 hit |
InterProi | View protein in InterPro IPR009056, Cyt_c-like_dom IPR036909, Cyt_c-like_dom_sf IPR002326, Cyt_c1 IPR021157, Cyt_c1_TM_anchor_C |
PANTHERi | PTHR10266, PTHR10266, 1 hit |
Pfami | View protein in Pfam PF02167, Cytochrom_C1, 1 hit |
PRINTSi | PR00603, CYTOCHROMEC1 |
SUPFAMi | SSF46626, SSF46626, 1 hit SSF81496, SSF81496, 1 hit |
PROSITEi | View protein in PROSITE PS51007, CYTC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CY1_BOVIN | |
Accessioni | P00125Primary (citable) accession number: P00125 Secondary accession number(s): Q2TBM2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | June 27, 2006 | |
Last modified: | February 23, 2022 | |
This is version 166 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families