UniProtKB - P00044 (CYC1_YEAST)
Protein
Cytochrome c isoform 1
Gene
CYC1
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Functioni
Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of ubiquinol-cytochrome c oxidoreductase. Cytochrome c then transfers this electron to the dinuclear copper A center (CU(A)) of the COX2 subunit of cytochrome oxidase, the final protein carrier in the mitochondrial electron-transport chain. Isoform 1 (CYC1) is the predominant cytochrome c during aerobic/normoxic growth.3 Publications
Miscellaneous
Present with 7330 molecules/cell in log phase SD medium.1 Publication
Cofactori
heme1 PublicationNote: Binds 1 heme group covalently per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 20 | Heme (covalent)Combined sources2 Publications | 1 | |
Binding sitei | 23 | Heme (covalent)Combined sources2 Publications | 1 | |
Metal bindingi | 24 | Iron (heme axial ligand); via tele nitrogenCombined sources2 Publications | 1 | |
Metal bindingi | 86 | Iron (heme axial ligand)Combined sources2 Publications | 1 |
GO - Molecular functioni
- electron transfer activity Source: SGD
- heme binding Source: InterPro
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- mitochondrial electron transport, cytochrome c to oxygen Source: SGD
- mitochondrial electron transport, ubiquinol to cytochrome c Source: SGD
Keywordsi
Biological process | Electron transport, Respiratory chain, Transport |
Ligand | Heme, Iron, Metal-binding |
Enzyme and pathway databases
Reactomei | R-SCE-111457, Release of apoptotic factors from the mitochondria R-SCE-3299685, Detoxification of Reactive Oxygen Species R-SCE-611105, Respiratory electron transport |
Protein family/group databases
MoonProti | P00044 |
Names & Taxonomyi
Protein namesi | Recommended name: Cytochrome c isoform 1Short name: Iso-1-cytochrome c Alternative name(s): Cytochrome c aerobic isoform |
Gene namesi | Name:CYC1 Ordered Locus Names:YJR048W ORF Names:J1653 |
Organismi | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) |
Taxonomic identifieri | 559292 [NCBI] |
Taxonomic lineagei | Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › |
Proteomesi |
|
Organism-specific databases
SGDi | S000003809, CYC1 |
VEuPathDBi | FungiDB:YJR048W |
Subcellular locationi
Mitochondrion
- Mitochondrion intermembrane space 2 Publications
Mitochondrion
- mitochondrial intermembrane space Source: SGD
- mitochondrion Source: SGD
Other locations
- respirasome Source: UniProtKB-KW
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 78 | K → R: Loss of methylation by CTM1. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed2 Publications | |||
ChainiPRO_0000108337 | 2 – 109 | Cytochrome c isoform 1Add BLAST | 108 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 78 | N6,N6,N6-trimethyllysine; by CTM1Combined sources2 Publications | 1 | |
Modified residuei | 79 | N6,N6,N6-trimethyllysine2 Publications | 1 |
Post-translational modificationi
Methylation may enhance its transport into mitochondria. Methylation occurs in fungi, plants, and some protozoa, but not in animals. The precise role of methylation at Lys-79 is unknown, but it seems to preclude pro-apoptotic activity, possibly by lowering affinity for APAF1.4 Publications
Keywords - PTMi
MethylationProteomic databases
MaxQBi | P00044 |
PaxDbi | P00044 |
PRIDEi | P00044 |
Expressioni
Inductioni
By oxygen at the level of transcription. Expression drops rapidly when the oxygen concentration falls below 0.5 µM O2.1 Publication
Interactioni
Binary interactionsi
P00044
With | #Exp. | IntAct |
---|---|---|
CCP1 [P00431] | 4 | EBI-5393,EBI-4389 |
Protein-protein interaction databases
BioGRIDi | 33801, 130 interactors |
DIPi | DIP-4265N |
IntActi | P00044, 4 interactors |
MINTi | P00044 |
STRINGi | 4932.YJR048W |
Miscellaneous databases
RNActi | P00044, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | P00044 |
SMRi | P00044 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | P00044 |
Family & Domainsi
Sequence similaritiesi
Belongs to the cytochrome c family.Curated
Phylogenomic databases
eggNOGi | KOG3453, Eukaryota |
GeneTreei | ENSGT00940000168884 |
HOGENOMi | CLU_060944_3_0_1 |
InParanoidi | P00044 |
OMAi | MPAPYEK |
Family and domain databases
Gene3Di | 1.10.760.10, 1 hit |
InterProi | View protein in InterPro IPR009056, Cyt_c-like_dom IPR036909, Cyt_c-like_dom_sf IPR002327, Cyt_c_1A/1B |
PANTHERi | PTHR11961, PTHR11961, 1 hit |
Pfami | View protein in Pfam PF00034, Cytochrom_C, 1 hit |
PRINTSi | PR00604, CYTCHRMECIAB |
SUPFAMi | SSF46626, SSF46626, 1 hit |
PROSITEi | View protein in PROSITE PS51007, CYTC, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
P00044-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTEFKAGSAK KGATLFKTRC LQCHTVEKGG PHKVGPNLHG IFGRHSGQAE
60 70 80 90 100
GYSYTDANIK KNVLWDENNM SEYLTNPKKY IPGTKMAFGG LKKEKDRNDL
ITYLKKACE
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37696 Genomic DNA Translation: AAB59344.1 V01298 Genomic DNA Translation: CAA24605.1 L26347 Genomic DNA Translation: AAA62856.1 L36344 Genomic DNA Translation: AAA88751.1 Z49548 Genomic DNA Translation: CAA89576.1 X03472 Genomic DNA Translation: CAA27189.1 BK006943 Genomic DNA Translation: DAA08835.1 |
PIRi | A00037, CCBY |
RefSeqi | NP_012582.1, NM_001181706.1 |
Genome annotation databases
EnsemblFungii | YJR048W_mRNA; YJR048W; YJR048W |
GeneIDi | 853507 |
KEGGi | sce:YJR048W |
Similar proteinsi
Cross-referencesi
Web resourcesi
Protein Spotlight Life shuttle - Issue 76 of November 2006 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | M37696 Genomic DNA Translation: AAB59344.1 V01298 Genomic DNA Translation: CAA24605.1 L26347 Genomic DNA Translation: AAA62856.1 L36344 Genomic DNA Translation: AAA88751.1 Z49548 Genomic DNA Translation: CAA89576.1 X03472 Genomic DNA Translation: CAA27189.1 BK006943 Genomic DNA Translation: DAA08835.1 |
PIRi | A00037, CCBY |
RefSeqi | NP_012582.1, NM_001181706.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1CHH | X-ray | 1.97 | A | 2-107 | [»] | |
1CHI | X-ray | 2.00 | A | 2-107 | [»] | |
1CHJ | X-ray | 1.90 | A | 2-107 | [»] | |
1CIE | X-ray | 1.80 | A | 2-107 | [»] | |
1CIF | X-ray | 1.90 | A | 2-107 | [»] | |
1CIG | X-ray | 1.80 | A | 2-107 | [»] | |
1CIH | X-ray | 1.80 | A | 2-107 | [»] | |
1CRG | X-ray | 2.00 | A | 2-107 | [»] | |
1CRH | X-ray | 1.90 | A | 2-109 | [»] | |
1CRI | X-ray | 2.00 | A | 2-107 | [»] | |
1CRJ | X-ray | 2.05 | A | 2-107 | [»] | |
1CSU | X-ray | 1.81 | A | 2-107 | [»] | |
1CSV | X-ray | 1.90 | A | 2-107 | [»] | |
1CSW | X-ray | 1.90 | A | 2-107 | [»] | |
1CSX | X-ray | 1.90 | A | 2-107 | [»] | |
1CTY | X-ray | 2.20 | A | 2-107 | [»] | |
1CTZ | X-ray | 1.90 | A | 2-107 | [»] | |
1FHB | NMR | - | A | 2-107 | [»] | |
1IRV | X-ray | 1.90 | A | 2-107 | [»] | |
1IRW | X-ray | 2.00 | A | 2-107 | [»] | |
1KYO | X-ray | 2.97 | W | 2-109 | [»] | |
1LMS | NMR | - | A | 2-107 | [»] | |
1NMI | NMR | - | A | 2-107 | [»] | |
1RAP | X-ray | 2.25 | A | 2-109 | [»] | |
1RAQ | X-ray | 1.90 | A | 2-109 | [»] | |
1S6V | X-ray | 1.88 | B/D | 2-107 | [»] | |
1U74 | X-ray | 2.40 | B/D | 2-107 | [»] | |
1YCC | X-ray | 1.23 | A | 2-109 | [»] | |
1YFC | NMR | - | A | 2-107 | [»] | |
1YIC | NMR | - | A | 2-107 | [»] | |
2B0Z | X-ray | 2.70 | B | 2-109 | [»] | |
2B10 | X-ray | 2.80 | B/D | 2-109 | [»] | |
2B11 | X-ray | 2.30 | B/D | 2-109 | [»] | |
2B12 | X-ray | 3.02 | B | 2-109 | [»] | |
2BCN | X-ray | 1.70 | B | 2-107 | [»] | |
2GB8 | NMR | - | B | 2-109 | [»] | |
2HV4 | NMR | - | A | 2-107 | [»] | |
2JQR | NMR | - | A | 2-107 | [»] | |
2JTI | NMR | - | B | 2-109 | [»] | |
2LIR | NMR | - | A | 2-107 | [»] | |
2LIT | NMR | - | A | 2-107 | [»] | |
2MHM | NMR | - | A | 2-107 | [»] | |
2N18 | NMR | - | B | 3-107 | [»] | |
C | 2-109 | [»] | ||||
2ORL | NMR | - | A | 2-107 | [»] | |
2PCC | X-ray | 2.30 | B/D | 2-109 | [»] | |
2YCC | X-ray | 1.90 | A | 2-107 | [»] | |
3CX5 | X-ray | 1.90 | W | 2-109 | [»] | |
3TYI | X-ray | 1.40 | A/B | 2-107 | [»] | |
4MU8 | X-ray | 1.45 | A/B | 2-107 | [»] | |
4N0K | X-ray | 1.05 | A/B | 2-107 | [»] | |
4P4Q | X-ray | 2.01 | B/D | 7-109 | [»] | |
4Q5P | X-ray | 1.87 | A/B/C | 4-107 | [»] | |
4QAO | X-ray | 2.10 | A/B/C | 4-107 | [»] | |
4YE1 | X-ray | 1.39 | A/B | 2-109 | [»] | |
5CIB | X-ray | 3.01 | B/D | 2-109 | [»] | |
5CIC | X-ray | 2.10 | B/D | 2-109 | [»] | |
5CID | X-ray | 2.76 | B/D | 2-109 | [»] | |
5CIE | X-ray | 2.60 | B/D | 2-109 | [»] | |
5CIF | X-ray | 2.01 | B/D | 7-109 | [»] | |
5CIG | X-ray | 2.06 | B/D | 2-109 | [»] | |
5CIH | X-ray | 2.60 | B/D | 7-109 | [»] | |
5KKE | X-ray | 1.70 | A | 2-107 | [»] | |
5KLU | X-ray | 1.99 | A/B | 4-107 | [»] | |
5KPF | X-ray | 1.70 | A/B | 3-109 | [»] | |
5LFT | X-ray | 1.25 | A | 3-109 | [»] | |
5LYC | X-ray | 1.80 | A/B | 3-109 | [»] | |
5NCV | X-ray | 1.50 | A/B | 3-109 | [»] | |
5T7H | X-ray | 2.00 | A/B/C/D | 1-107 | [»] | |
5T8W | X-ray | 1.60 | A/B | 2-109 | [»] | |
6EGY | X-ray | 2.70 | A/B | 2-109 | [»] | |
6EGZ | X-ray | 2.17 | A/B | 2-109 | [»] | |
6GD6 | X-ray | 1.20 | A | 3-109 | [»] | |
6GD7 | X-ray | 1.55 | A | 3-109 | [»] | |
6GD8 | X-ray | 2.50 | A/B/C/D | 3-109 | [»] | |
6GD9 | X-ray | 2.65 | A | 3-109 | [»] | |
6GDA | X-ray | 2.80 | A | 3-109 | [»] | |
6P41 | X-ray | 2.90 | B/D | 7-109 | [»] | |
6P42 | X-ray | 2.90 | B/D | 7-109 | [»] | |
6P43 | X-ray | 1.91 | B | 2-109 | [»] | |
6RGI | X-ray | 2.64 | A | 3-109 | [»] | |
6RSI | X-ray | 2.48 | A | 3-109 | [»] | |
6RSJ | X-ray | 2.27 | A | 3-109 | [»] | |
6RSK | X-ray | 2.31 | A/B | 3-109 | [»] | |
6RSL | X-ray | 1.99 | A/B | 3-109 | [»] | |
6S8Y | X-ray | 2.09 | A | 3-109 | [»] | |
6SUY | X-ray | 1.75 | A/B | 2-109 | [»] | |
BMRBi | P00044 | |||||
SMRi | P00044 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 33801, 130 interactors |
DIPi | DIP-4265N |
IntActi | P00044, 4 interactors |
MINTi | P00044 |
STRINGi | 4932.YJR048W |
Protein family/group databases
MoonProti | P00044 |
Proteomic databases
MaxQBi | P00044 |
PaxDbi | P00044 |
PRIDEi | P00044 |
Genome annotation databases
EnsemblFungii | YJR048W_mRNA; YJR048W; YJR048W |
GeneIDi | 853507 |
KEGGi | sce:YJR048W |
Organism-specific databases
SGDi | S000003809, CYC1 |
VEuPathDBi | FungiDB:YJR048W |
Phylogenomic databases
eggNOGi | KOG3453, Eukaryota |
GeneTreei | ENSGT00940000168884 |
HOGENOMi | CLU_060944_3_0_1 |
InParanoidi | P00044 |
OMAi | MPAPYEK |
Enzyme and pathway databases
Reactomei | R-SCE-111457, Release of apoptotic factors from the mitochondria R-SCE-3299685, Detoxification of Reactive Oxygen Species R-SCE-611105, Respiratory electron transport |
Miscellaneous databases
EvolutionaryTracei | P00044 |
PROi | PR:P00044 |
RNActi | P00044, protein |
Family and domain databases
Gene3Di | 1.10.760.10, 1 hit |
InterProi | View protein in InterPro IPR009056, Cyt_c-like_dom IPR036909, Cyt_c-like_dom_sf IPR002327, Cyt_c_1A/1B |
PANTHERi | PTHR11961, PTHR11961, 1 hit |
Pfami | View protein in Pfam PF00034, Cytochrom_C, 1 hit |
PRINTSi | PR00604, CYTCHRMECIAB |
SUPFAMi | SSF46626, SSF46626, 1 hit |
PROSITEi | View protein in PROSITE PS51007, CYTC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CYC1_YEAST | |
Accessioni | P00044Primary (citable) accession number: P00044 Secondary accession number(s): D6VWL9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 21, 1986 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 221 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Fungal Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- Yeast
Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD - Yeast chromosome X
Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families - Protein Spotlight
Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries