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Protein

Cytochrome c

Gene

CYCS

Organism
Equus caballus (Horse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain.
Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases (By similarity).By similarity

Miscellaneous

Mules and hinnies are heterozygous, having equal amount of horse and donkey cytochromes c.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei15Heme (covalent)1
Binding sitei18Heme (covalent)1
Metal bindingi19Iron (heme axial ligand)PROSITE-ProRule annotation1 Publication1
Metal bindingi81Iron (heme axial ligand)1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processApoptosis, Electron transport, Respiratory chain, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

SABIO-RKiP00004

Protein family/group databases

MoonProtiP00004

Names & Taxonomyi

Protein namesi
Recommended name:
Cytochrome c
Gene namesi
Name:CYCS
Synonyms:CYC
OrganismiEquus caballus (Horse)
Taxonomic identifieri9796 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaPerissodactylaEquidaeEquus
Proteomesi
  • UP000002281 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001082172 – 105Cytochrome cAdd BLAST104

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycine1 Publication1
Modified residuei49PhosphotyrosineBy similarity1
Modified residuei56N6-succinyllysineBy similarity1
Modified residuei73N6-acetyllysine; alternateBy similarity1
Modified residuei73N6-succinyllysine; alternateBy similarity1
Modified residuei98PhosphotyrosineBy similarity1
Modified residuei100N6-acetyllysineBy similarity1

Post-translational modificationi

Binds 1 heme group per subunit.
Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP00004
PeptideAtlasiP00004
PRIDEiP00004

PTM databases

CarbonylDBiP00004
iPTMnetiP00004

Miscellaneous databases

PMAP-CutDBiP00004

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-865260,EBI-865260

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-36774N
IntActiP00004, 2 interactors
STRINGi9796.ENSECAP00000012031

Structurei

Secondary structure

1105
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

DisProtiDP00006
ProteinModelPortaliP00004
SMRiP00004
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP00004

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome c family.Curated

Phylogenomic databases

eggNOGiKOG3453 Eukaryota
COG3474 LUCA
HOGENOMiHOG000009762
HOVERGENiHBG003023
InParanoidiP00004

Family and domain databases

Gene3Di1.10.760.10, 1 hit
InterProiView protein in InterPro
IPR009056 Cyt_c-like_dom
IPR036909 Cyt_c-like_dom_sf
IPR002327 Cyt_c_1A/1B
PANTHERiPTHR11961 PTHR11961, 1 hit
PfamiView protein in Pfam
PF00034 Cytochrom_C, 1 hit
PRINTSiPR00604 CYTCHRMECIAB
SUPFAMiSSF46626 SSF46626, 1 hit
PROSITEiView protein in PROSITE
PS51007 CYTC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

P00004-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGDVEKGKKI FVQKCAQCHT VEKGGKHKTG PNLHGLFGRK TGQAPGFTYT
60 70 80 90 100
DANKNKGITW KEETLMEYLE NPKKYIPGTK MIFAGIKKKT EREDLIAYLK

KATNE
Length:105
Mass (Da):11,833
Last modified:January 23, 2007 - v2
Checksum:i659BA128E53C3868
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F7D4V9F7D4V9_HORSE
Cytochrome c
CYCS
105Annotation score:

Sequence databases

PIRiA00005 CCHO
UniGeneiEca.1571

Similar proteinsi

Cross-referencesi

Web resourcesi

Protein Spotlight

Life shuttle - Issue 76 of November 2006

Sequence databases

PIRiA00005 CCHO
UniGeneiEca.1571

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AKKNMR-A2-105[»]
1CRCX-ray2.08A/B2-105[»]
1FI7NMR-A2-105[»]
1FI9NMR-A2-105[»]
1GIWNMR-A2-105[»]
1HRCX-ray1.90A2-105[»]
1I5TNMR-A2-105[»]
1LC1NMR-A2-105[»]
1LC2NMR-A2-105[»]
1M60NMR-A2-105[»]
1OCDNMR-A2-105[»]
1U75X-ray2.55B2-105[»]
1WEJX-ray1.80F2-105[»]
2FRCNMR-A2-105[»]
2GIWNMR-A2-105[»]
2N3BNMR-A2-105[»]
2PCBX-ray2.80B2-105[»]
3JBTelectron microscopy3.80B/D/F/H/J/L/N1-105[»]
3NBSX-ray2.20A/B/C/D2-105[»]
3NBTX-ray2.10A/B/C/D/E/F2-105[»]
3O1YX-ray1.75A/B/C2-105[»]
3O20X-ray1.90A/B/C2-105[»]
3WC8X-ray1.80A2-105[»]
3WUIX-ray1.80A2-105[»]
4NFGX-ray2.11B2-105[»]
4RSZX-ray2.19A/B/C/D/E/F2-105[»]
5IY5X-ray2.001/22-105[»]
5WVEelectron microscopy4.40B/D/F/H/J/L/N1-105[»]
DisProtiDP00006
ProteinModelPortaliP00004
SMRiP00004
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-36774N
IntActiP00004, 2 interactors
STRINGi9796.ENSECAP00000012031

Protein family/group databases

MoonProtiP00004

PTM databases

CarbonylDBiP00004
iPTMnetiP00004

Proteomic databases

PaxDbiP00004
PeptideAtlasiP00004
PRIDEiP00004

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiKOG3453 Eukaryota
COG3474 LUCA
HOGENOMiHOG000009762
HOVERGENiHBG003023
InParanoidiP00004

Enzyme and pathway databases

SABIO-RKiP00004

Miscellaneous databases

EvolutionaryTraceiP00004
PMAP-CutDBiP00004

Family and domain databases

Gene3Di1.10.760.10, 1 hit
InterProiView protein in InterPro
IPR009056 Cyt_c-like_dom
IPR036909 Cyt_c-like_dom_sf
IPR002327 Cyt_c_1A/1B
PANTHERiPTHR11961 PTHR11961, 1 hit
PfamiView protein in Pfam
PF00034 Cytochrom_C, 1 hit
PRINTSiPR00604 CYTCHRMECIAB
SUPFAMiSSF46626 SSF46626, 1 hit
PROSITEiView protein in PROSITE
PS51007 CYTC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCYC_HORSE
AccessioniPrimary (citable) accession number: P00004
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 21, 1986
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 143 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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