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Protein

Amiloride-sensitive sodium channel subunit beta

Gene

SCNN1B

Organism
Oryctolagus cuniculus (Rabbit)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Plays an essential role in electrolyte and blood pressure homeostasis, but also in airway surface liquid homeostasis, which is important for proper clearance of mucus. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.By similarity

Activity regulationi

Activated by WNK1, WNK2, WNK3 and WNK4.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Sodium channel
Biological processIon transport, Sensory transduction, Sodium transport, Taste, Transport
LigandSodium

Names & Taxonomyi

Protein namesi
Recommended name:
Amiloride-sensitive sodium channel subunit beta
Alternative name(s):
Beta-NaCH
Epithelial Na(+) channel subunit beta
Short name:
Beta-ENaC
Nonvoltage-gated sodium channel 1 subunit beta
SCNEB
Gene namesi
Name:SCNN1B
OrganismiOryctolagus cuniculus (Rabbit)
Taxonomic identifieri9986 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresLagomorphaLeporidaeOryctolagus
Proteomesi
  • UP000001811 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 50CytoplasmicBy similarityAdd BLAST50
Transmembranei51 – 71Helical; Name=1Sequence analysisAdd BLAST21
Topological domaini72 – 533ExtracellularBy similarityAdd BLAST462
Transmembranei534 – 554Helical; Name=2Sequence analysisAdd BLAST21
Topological domaini555 – 641CytoplasmicBy similarityAdd BLAST87

Keywords - Cellular componenti

Cell membrane, Cytoplasmic vesicle, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001812701 – 641Amiloride-sensitive sodium channel subunit betaAdd BLAST641

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi141N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi261N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi379N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei634PhosphoserineBy similarity1
Modified residuei636PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated on serine and threonine residues. Aldosterone and insulin increase the basal level of phosphorylation.By similarity
N-glycosylated. N-glycosylation is required for interaction with BPIFA1.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiO97742

Interactioni

Subunit structurei

Heterotrimer containing an alpha/SCNN1A, a beta/SCNN1B and a gamma/SCNN1G subunit. An additional delta/SCNN1D subunit exists only in some organisms and can replace the alpha/SCNN1A subunit to form an alternative channel with specific properties. Interacts with NEDD4 (via WW domains). Interacts with NEDD4L (via WW domains). Interacts with WWP1 (via WW domains). Interacts with WWP2 (via WW domains). Interacts with the full-length immature form of PCSK9 (pro-PCSK9). Interacts (N-glycosylated) with BPIFA1; the interaction is direct and inhibits the proteolytic processing of SCNN1A and SCNN1G and the activation of ENaC.By similarity

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000236286
HOVERGENiHBG058435
InParanoidiO97742
KOiK04825

Family and domain databases

InterProiView protein in InterPro
IPR001873 ENaC
IPR004724 ENaC_chordates
IPR020903 ENaC_CS
PANTHERiPTHR11690 PTHR11690, 1 hit
PfamiView protein in Pfam
PF00858 ASC, 1 hit
PRINTSiPR01078 AMINACHANNEL
TIGRFAMsiTIGR00859 ENaC, 1 hit
PROSITEiView protein in PROSITE
PS01206 ASC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.iShow all

O97742-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MHVKKYLLKC LHRLQKGPGY TYKELLVWYC DNTNTHGPKR IIREGPKKKA
60 70 80 90 100
MWFLITLLFA SLVCWQWGVF IKTYLSWEVS VSLSMGFKAM DFPAVTICNA
110 120 130 140 150
SPFRYSKVRH LLKDLDELME AVLARILAPE SSQANATAAM NLSMWNYTPL
160 170 180 190 200
VLIDERDPHH PVVLDLFAND PTGSASSSPG PRGACNAQGC KVAMRLCSLN
210 220 230 240 250
GTSCTFRNFS SATQAVTEWY ALQATNIFSQ VPQRELVELG YSAERLILAC
260 270 280 290 300
LFGAEPCSYR NFTSIFYPDY GNCYVFNWGM TEKALPSANP GTEFGLKLIL
310 320 330 340 350
DIGQEDYVPF LASTAGVRLM LHEQRSYPFI REEGVDAMSG TETSIGVLVD
360 370 380 390 400
KLQRKGEPYS PCTVNGSDVP VQNLYGSYNT TYSIQACLHS CFQTQMIASC
410 420 430 440 450
QCGHYLYPLP RGQEYCNSRD FPDWAPCYLA LRMSEAERET CIRMCKESCN
460 470 480 490 500
DTQYKMTISM ADWPSEASED WIFHVLSQER DQSTNVTLSR KGVVKLNIYF
510 520 530 540 550
QEFNYRTIEE SAANNIVWLL SNLGGQFGFW MGGSVLCLIE FAEIIIDFVW
560 570 580 590 600
ITIIKLVALA KGLRQRQAQA RYAGPPPTVA ELVEAHTNFG FQPDVGHRSP
610 620 630 640
DAEAYPDEQA LPIPGTPPPN YDSLRLQPLD VVESDSEGDA V
Length:641
Mass (Da):71,973
Last modified:May 1, 1999 - v1
Checksum:i1FBE6181B232AD06
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G1SV30G1SV30_RABIT
Amiloride-sensitive sodium channel ...
SCNN1B
773Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti275V → I in AAF43681 (Ref. 2) Curated1
Sequence conflicti389H → R in AAF43681 (Ref. 2) Curated1
Sequence conflicti415Y → H in AAF43681 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ132109 mRNA Translation: CAA10572.1
AF229026 mRNA Translation: AAF43681.1
RefSeqiNP_001076244.1, NM_001082775.1
UniGeneiOcu.2597

Genome annotation databases

GeneIDi100009564
KEGGiocu:100009564

Similar proteinsi

Entry informationi

Entry nameiSCNNB_RABIT
AccessioniPrimary (citable) accession number: O97742
Secondary accession number(s): Q9N132
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: September 12, 2018
This is version 100 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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