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UniProtKB - O96013 (PAK4_HUMAN)

Entry version 220 (23 Feb 2022)
Sequence version 1 (01 May 1999)
Previous versions | rss
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Protein

Serine/threonine-protein kinase PAK 4

Gene

PAK4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, growth, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates and inactivates the protein phosphatase SSH1, leading to increased inhibitory phosphorylation of the actin binding/depolymerizing factor cofilin. Decreased cofilin activity may lead to stabilization of actin filaments. Phosphorylates LIMK1, a kinase that also inhibits the activity of cofilin. Phosphorylates integrin beta5/ITGB5 and thus regulates cell motility. Phosphorylates ARHGEF2 and activates the downstream target RHOA that plays a role in the regulation of assembly of focal adhesions and actin stress fibers. Stimulates cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Alternatively, inhibits apoptosis by preventing caspase-8 binding to death domain receptors in a kinase independent manner. Plays a role in cell-cycle progression by controlling levels of the cell-cycle regulatory protein CDKN1A and by phosphorylating RAN.

8 Publications

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the catalytic activity of an enzyme, i.e. a chemical reaction that the enzyme catalyzes.<p><a href='/help/catalytic_activity' target='_top'>More...</a></p>Catalytic activityi

<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes regulatory mechanisms for enzymes, transporters or microbial transcription factors, and reports the components which regulate (by activation or inhibition) the reaction.<p><a href='/help/activity_regulation' target='_top'>More...</a></p>Activity regulationi

Inhibited by INKA1; which inhibits the serine/threonine-protein kinase activity by binding PAK4 in a substrate-like manner (PubMed:26607847).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei350ATPPROSITE-ProRule annotationCombined sources1 Publication1
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section is used for enzymes and indicates the residues directly involved in catalysis.<p><a href='/help/act_site' target='_top'>More...</a></p>Active sitei440Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi327 – 335ATPPROSITE-ProRule annotationCombined sources1 Publication9
Nucleotide bindingi396 – 398ATPCombined sources1 Publication3
Nucleotide bindingi458 – 460ATPCombined sources1 Publication3

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processApoptosis, Cell cycle
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDA Comprehensive Enzyme Information System

More...BRENDAi		2.7.11.1, 2681

Pathway Commons web resource for biological pathway data

More...PathwayCommonsi		O96013

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-HSA-428540, Activation of RAC1
R-HSA-9013148, CDC42 GTPase cycle
R-HSA-9013149, RAC1 GTPase cycle
R-HSA-9013404, RAC2 GTPase cycle
R-HSA-9013406, RHOQ GTPase cycle
R-HSA-9013407, RHOH GTPase cycle
R-HSA-9013408, RHOG GTPase cycle
R-HSA-9013409, RHOJ GTPase cycle
R-HSA-9013420, RHOU GTPase cycle
R-HSA-9013423, RAC3 GTPase cycle
R-HSA-9013424, RHOV GTPase cycle

SignaLink: a signaling pathway resource with multi-layered regulatory networks

More...SignaLinki		O96013

SIGNOR Signaling Network Open Resource

More...SIGNORi		O96013

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Serine/threonine-protein kinase PAK 4 (EC:2.7.11.1)
Alternative name(s):
p21-activated kinase 4
Short name:
PAK-4
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:PAK4
Synonyms:KIAA1142
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiHomo sapiens (Human)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri9606 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000005640 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 19

Organism-specific databases

Human Gene Nomenclature Database

More...HGNCi		HGNC:16059, PAK4

Online Mendelian Inheritance in Man (OMIM)

More...MIMi		605451, gene

neXtProt; the human protein knowledge platform

More...neXtProti		NX_O96013

Eukaryotic Pathogen, Vector and Host Database Resources

More...VEuPathDBi		HostDB:ENSG00000130669

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/manual/pathology%5Fand%5Fbiotech%5Fsection">'Pathology and Biotech'</a> section describes the effect of the experimental mutation of one or more amino acid(s) on the biological properties of the protein.<p><a href='/help/mutagen' target='_top'>More...</a></p>Mutagenesisi445S → N: Approximately 30-fold increased autophosphorylation (constitutively active mutant). 1 Publication1
Mutagenesisi474S → E: Approximately 3-fold increased autophosphorylation. 1 Publication1

Organism-specific databases

DisGeNET

More...DisGeNETi		10298

Open Targets

More...OpenTargetsi		ENSG00000130669

The Pharmacogenetics and Pharmacogenomics Knowledge Base

More...PharmGKBi		PA32920

Miscellaneous databases

Pharos NIH Druggable Genome Knowledgebase

More...Pharosi		O96013, Tchem

Chemistry databases

ChEMBL database of bioactive drug-like small molecules

More...ChEMBLi		CHEMBL4482

Drug and drug target database

More...DrugBanki		DB12010, Fostamatinib

DrugCentral

More...DrugCentrali		O96013

IUPHAR/BPS Guide to PHARMACOLOGY

More...GuidetoPHARMACOLOGYi		2136

Genetic variation databases

BioMuta curated single-nucleotide variation and disease association database

More...BioMutai		PAK4

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000864741 – 591Serine/threonine-protein kinase PAK 4Add BLAST591

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei41PhosphoserineCombined sources1
Modified residuei78N6-methyllysineCombined sources1
Modified residuei104PhosphoserineCombined sources1
Modified residuei148PhosphoserineCombined sources1
Modified residuei167PhosphoserineCombined sources1
Modified residuei181PhosphoserineCombined sources1
Modified residuei187PhosphothreonineCombined sources1
Modified residuei195PhosphoserineCombined sources1
Modified residuei207PhosphothreonineCombined sources1
Modified residuei258PhosphoserineCombined sources1
Modified residuei267PhosphoserineCombined sources1
Modified residuei291PhosphoserineCombined sources1
Modified residuei474Phosphoserine; by autocatalysisCombined sources1 Publication1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Autophosphorylated on serine residues when activated by CDC42/p21 (Ref. 32). Phosphorylated on tyrosine residues upon stimulation of FGFR2 (By similarity). Methylated by SETD6.By similarity2 Publications
Polyubiquitinated, leading to its proteasomal degradation.1 Publication

Keywords - PTMi

Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		O96013

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		O96013

MassIVE - Mass Spectrometry Interactive Virtual Environment

More...MassIVEi		O96013

MaxQB - The MaxQuant DataBase

More...MaxQBi		O96013

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		O96013

PeptideAtlas

More...PeptideAtlasi		O96013

PRoteomics IDEntifications database

More...PRIDEi		O96013

ProteomicsDB: a multi-organism proteome resource

More...ProteomicsDBi		51192 [O96013-1]
51193 [O96013-2]
51194 [O96013-3]
51195 [O96013-4]

PTM databases

GlyGen: Computational and Informatics Resources for Glycoscience

More...GlyGeni		O96013, 1 site, 1 O-linked glycan (1 site)

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		O96013

MetOSite database of methionine sulfoxide sites

More...MetOSitei		O96013

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		O96013

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Highest expression in prostate, testis and colon.

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSG00000130669, Expressed in type B pancreatic cell and 245 other tissues

ExpressionAtlas, Differential and Baseline Expression

More...ExpressionAtlasi		O96013, baseline and differential

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		O96013, HS

Organism-specific databases

Human Protein Atlas

More...HPAi		ENSG00000130669, Low tissue specificity

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Interacts with FGFR2 and GRB2 (By similarity).

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and weakly with RAC1 (PubMed:15827085).

Interacts with INKA1 (PubMed:26607847).

Interacts with SH3RF2 (PubMed:24130170).

By similarity3 Publications

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Hide details

GO - Molecular functioni

Complete GO annotation on QuickGO ...

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		115586, 118 interactors

Database of interacting proteins

More...DIPi		DIP-39742N

Protein interaction database and analysis system

More...IntActi		O96013, 323 interactors

Molecular INTeraction database

More...MINTi		O96013

STRING: functional protein association networks

More...STRINGi		9606.ENSP00000469413

Chemistry databases

BindingDB database of measured binding affinities

More...BindingDBi		O96013

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		O96013, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

Secondary structure

1591
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		O96013

Database of comparative protein structure models

More...ModBasei		Search...

Protein Data Bank in Europe - Knowledge Base

More...PDBe-KBi		Search...

Miscellaneous databases

Relative evolutionary importance of amino acids within a protein sequence

More...EvolutionaryTracei		O96013

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/family%5Fand%5Fdomains%5Fsection">Family and Domains</a> section describes the position and type of a domain, which is defined as a specific combination of secondary structures organized into a characteristic three-dimensional structure or fold.<p><a href='/help/domain' target='_top'>More...</a></p>Domaini11 – 24CRIBPROSITE-ProRule annotationAdd BLAST14
Domaini321 – 572Protein kinasePROSITE-ProRule annotationAdd BLAST252

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni25 – 320LinkerAdd BLAST296
Regioni95 – 301DisorderedSequence analysisAdd BLAST207
Regioni298 – 323GEF-interaction domain (GID)Add BLAST26

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular type of amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi146 – 165Basic and acidic residuesSequence analysisAdd BLAST20
Compositional biasi236 – 252Polar residuesSequence analysisAdd BLAST17
Compositional biasi274 – 293Pro residuesSequence analysisAdd BLAST20

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.Curated

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG0578, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT01040000240717

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_000288_26_6_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		O96013

Identification of Orthologs from Complete Genome Data

More...OMAi		VCIATMK

Database for complete collections of gene phylogenies

More...PhylomeDBi		O96013

TreeFam database of animal gene trees

More...TreeFami		TF105352

Family and domain databases

Conserved Domains Database

More...CDDi		cd01093, CRIB_PAK_like, 1 hit

Database of protein disorder

More...DisProti		DP01184

Gene3D Structural and Functional Annotation of Protein Families

More...Gene3Di		3.90.810.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR000095, CRIB_dom
IPR036936, CRIB_dom_sf
IPR011009, Kinase-like_dom_sf
IPR033923, PAK_BD
IPR000719, Prot_kinase_dom
IPR017441, Protein_kinase_ATP_BS

Pfam protein domain database

More...Pfami		View protein in Pfam
PF00786, PBD, 1 hit
PF00069, Pkinase, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...SMARTi		View protein in SMART
SM00285, PBD, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF56112, SSF56112, 1 hit

PROSITE; a protein domain and family database

More...PROSITEi		View protein in PROSITE
PS50108, CRIB, 1 hit
PS00107, PROTEIN_KINASE_ATP, 1 hit
PS50011, PROTEIN_KINASE_DOM, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (4+)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 4 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 4 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: O96013-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <p><strong>What is the canonical sequence?</strong><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MFGKRKKRVE ISAPSNFEHR VHTGFDQHEQ KFTGLPRQWQ SLIEESARRP 
        60         70         80         90        100
KPLVDPACIT SIQPGAPKTI VRGSKGAKDG ALTLLLDEFE NMSVTRSNSL 
       110        120        130        140        150
RRDSPPPPAR ARQENGMPEE PATTARGGPG KAGSRGRFAG HSEAGGGSGD 
       160        170        180        190        200
RRRAGPEKRP KSSREGSGGP QESSRDKRPL SGPDVGTPQP AGLASGAKLA 
       210        220        230        240        250
AGRPFNTYPR ADTDHPSRGA QGEPHDVAPN GPSAGGLAIP QSSSSSSRPP 
       260        270        280        290        300
TRARGAPSPG VLGPHASEPQ LAPPACTPAA PAVPGPPGPR SPQREPQRVS 
       310        320        330        340        350
HEQFRAALQL VVDPGDPRSY LDNFIKIGEG STGIVCIATV RSSGKLVAVK 
       360        370        380        390        400
KMDLRKQQRR ELLFNEVVIM RDYQHENVVE MYNSYLVGDE LWVVMEFLEG 
       410        420        430        440        450
GALTDIVTHT RMNEEQIAAV CLAVLQALSV LHAQGVIHRD IKSDSILLTH 
       460        470        480        490        500
DGRVKLSDFG FCAQVSKEVP RRKSLVGTPY WMAPELISRL PYGPEVDIWS 
       510        520        530        540        550
LGIMVIEMVD GEPPYFNEPP LKAMKMIRDN LPPRLKNLHK VSPSLKGFLD 
       560        570        580        590 
RLLVRDPAQR ATAAELLKHP FLAKAGPPAS IVPLMRQNRT R
Length:591
Mass (Da):64,072
Last modified:May 1, 1999 - v1
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i04C2A5C0B06427D5
GO
Isoform 2 (identifier: O96013-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     120-120: E → K
     121-285: Missing.

Show »
Length:426
Mass (Da):47,924
Checksum:i505879690593E93A
GO
Isoform 3 (identifier: O96013-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     69-221: Missing.

Show »
Length:438
Mass (Da):48,268
Checksum:i4A596EBBCECE9883
GO
Isoform 4 (identifier: O96013-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     132-221: Missing.

Show »
Length:501
Mass (Da):54,940
Checksum:i6EE6240ECE65E79D
GO

<p>In eukaryotic reference proteomes, unreviewed entries that are likely to belong to the same gene are computationally mapped, based on gene identifiers from Ensembl, EnsemblGenomes and model organism databases.<p><a href='/help/gene_centric_isoform_mapping' target='_top'>More...</a></p>Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
M0R0L9M0R0L9_HUMAN
Non-specific serine/threonine prote...
PAK4
182Annotation score:

Annotation score:2 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R2X4M0R2X4_HUMAN
Non-specific serine/threonine prote...
PAK4
131Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R3G6M0R3G6_HUMAN
Non-specific serine/threonine prote...
PAK4
93Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
M0R1R1M0R1R1_HUMAN
Serine/threonine-protein kinase PAK...
PAK4
149Annotation score:

Annotation score:1 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>

<p>This subsection of the 'Sequence' section reports difference(s) between the protein sequence shown in the UniProtKB entry and other available protein sequences derived from the same gene.<p><a href='/help/sequence_caution' target='_top'>More...</a></p>Sequence cautioni

The sequence BAA86456 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAC11166 differs from that shown. Reason: Erroneous initiation.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes natural variant(s) of the protein sequence.<p><a href='/help/variant' target='_top'>More...</a></p>Natural variantiVAR_040970135R → Q1 PublicationCorresponds to variant dbSNP:rs56099436Ensembl.1
Natural variantiVAR_040971139A → T1 PublicationCorresponds to variant dbSNP:rs35655056Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_01757269 – 221Missing in isoform 3. 3 PublicationsAdd BLAST153
Alternative sequenceiVSP_004892120E → K in isoform 2. 1 Publication1
Alternative sequenceiVSP_004893121 – 285Missing in isoform 2. 1 PublicationAdd BLAST165
Alternative sequenceiVSP_017573132 – 221Missing in isoform 4. 1 PublicationAdd BLAST90

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AJ011855 mRNA Translation: CAA09820.1
AF005046 mRNA Translation: AAD01210.1
AB032968 mRNA Translation: BAA86456.1 Different initiation.
AK074728 mRNA Translation: BAC11166.1 Different initiation.
AK294586 mRNA Translation: BAG57777.1
AL834236 mRNA Translation: CAD38914.2
CH471126 Genomic DNA Translation: EAW56861.1
BC002921 mRNA Translation: AAH02921.1
BC011368 mRNA Translation: AAH11368.1
BC025282 mRNA Translation: AAH25282.1
BC034511 mRNA Translation: AAH34511.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS12528.1 [O96013-1]
CCDS33019.1 [O96013-3]

NCBI Reference Sequences

More...RefSeqi		NP_001014831.1, NM_001014831.2 [O96013-1]
NP_001014832.1, NM_001014832.1 [O96013-1]
NP_001014834.1, NM_001014834.2 [O96013-3]
NP_001014835.1, NM_001014835.1 [O96013-3]
NP_005875.1, NM_005884.3 [O96013-1]
XP_011524618.1, XM_011526316.1 [O96013-1]
XP_011524619.1, XM_011526317.2 [O96013-1]
XP_011524620.1, XM_011526318.2 [O96013-1]
XP_011524621.1, XM_011526319.2 [O96013-1]
XP_011524622.1, XM_011526320.1 [O96013-3]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENST00000321944; ENSP00000326864; ENSG00000130669 [O96013-4]
ENST00000358301; ENSP00000351049; ENSG00000130669
ENST00000360442; ENSP00000353625; ENSG00000130669
ENST00000593690; ENSP00000469413; ENSG00000130669
ENST00000599386; ENSP00000471157; ENSG00000130669 [O96013-3]
ENST00000599470; ENSP00000470284; ENSG00000130669 [O96013-3]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		10298

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		hsa:10298

UCSC genome browser

More...UCSCi		uc002okj.2, human [O96013-1]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ011855 mRNA Translation: CAA09820.1
AF005046 mRNA Translation: AAD01210.1
AB032968 mRNA Translation: BAA86456.1 Different initiation.
AK074728 mRNA Translation: BAC11166.1 Different initiation.
AK294586 mRNA Translation: BAG57777.1
AL834236 mRNA Translation: CAD38914.2
CH471126 Genomic DNA Translation: EAW56861.1
BC002921 mRNA Translation: AAH02921.1
BC011368 mRNA Translation: AAH11368.1
BC025282 mRNA Translation: AAH25282.1
BC034511 mRNA Translation: AAH34511.1
CCDSiCCDS12528.1 [O96013-1]
CCDS33019.1 [O96013-3]
RefSeqiNP_001014831.1, NM_001014831.2 [O96013-1]
NP_001014832.1, NM_001014832.1 [O96013-1]
NP_001014834.1, NM_001014834.2 [O96013-3]
NP_001014835.1, NM_001014835.1 [O96013-3]
NP_005875.1, NM_005884.3 [O96013-1]
XP_011524618.1, XM_011526316.1 [O96013-1]
XP_011524619.1, XM_011526317.2 [O96013-1]
XP_011524620.1, XM_011526318.2 [O96013-1]
XP_011524621.1, XM_011526319.2 [O96013-1]
XP_011524622.1, XM_011526320.1 [O96013-3]

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...PDBei

Protein Data Bank RCSB

More...RCSB PDBi

Protein Data Bank Japan

More...PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BVAX-ray2.30A/B300-591[»]
2CDZX-ray2.30A291-591[»]
2J0IX-ray1.60A291-591[»]
2OV2X-ray2.10I/J/K/L/M/N/O/P10-44[»]
2Q0NX-ray1.75A291-591[»]
2X4ZX-ray2.10A297-591[»]
4APPX-ray2.20A300-591[»]
4FIEX-ray3.11A/B5-591[»]
4FIFX-ray2.60A/B286-591[»]
C/D49-56[»]
4FIGX-ray3.01A/B286-591[»]
4FIHX-ray1.97A286-591[»]
4FIIX-ray2.00A286-591[»]
B49-56[»]
4FIJX-ray2.30A286-591[»]
4JDHX-ray2.00A286-591[»]
4JDIX-ray1.85A286-591[»]
4JDJX-ray2.30A286-591[»]
4JDKX-ray2.40A286-591[»]
4L67X-ray2.80A300-591[»]
B36-60[»]
4NJDX-ray2.50A300-591[»]
4O0VX-ray2.80A300-591[»]
4O0XX-ray2.48A300-591[»]
4O0YX-ray2.20A300-591[»]
4XBRX-ray2.94A278-591[»]
4XBUX-ray2.06A286-591[»]
5BMSX-ray2.90A300-591[»]
5I0BX-ray3.09A300-591[»]
5UPKX-ray2.40A1-45[»]
B286-591[»]
5UPLX-ray3.00A2-591[»]
5VEDX-ray2.30A286-591[»]
5VEEX-ray2.50A286-591[»]
5VEFX-ray1.75A286-591[»]
5XVAX-ray1.85A300-591[»]
5XVFX-ray2.65A300-588[»]
5XVGX-ray2.10A300-591[»]
5ZJWX-ray1.80A300-591[»]
6WLXX-ray2.20A286-591[»]
6WLYX-ray1.90A286-591[»]
7CMBX-ray2.59A300-591[»]
7CP3X-ray2.90A300-591[»]
7CP4X-ray2.50A300-591[»]
SMRiO96013
ModBaseiSearch...
PDBe-KBiSearch...

Protein-protein interaction databases

BioGRIDi115586, 118 interactors
DIPiDIP-39742N
IntActiO96013, 323 interactors
MINTiO96013
STRINGi9606.ENSP00000469413

Chemistry databases

BindingDBiO96013
ChEMBLiCHEMBL4482
DrugBankiDB12010, Fostamatinib
DrugCentraliO96013
GuidetoPHARMACOLOGYi2136

PTM databases

GlyGeniO96013, 1 site, 1 O-linked glycan (1 site)
iPTMnetiO96013
MetOSiteiO96013
PhosphoSitePlusiO96013

Genetic variation databases

BioMutaiPAK4

Proteomic databases

EPDiO96013
jPOSTiO96013
MassIVEiO96013
MaxQBiO96013
PaxDbiO96013
PeptideAtlasiO96013
PRIDEiO96013
ProteomicsDBi51192 [O96013-1]
51193 [O96013-2]
51194 [O96013-3]
51195 [O96013-4]

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		16732, 628 antibodies from 43 providers

The CPTC Antibody Portal

More...CPTCi		O96013, 1 antibody

The DNASU plasmid repository

More...DNASUi		10298

Genome annotation databases

EnsembliENST00000321944; ENSP00000326864; ENSG00000130669 [O96013-4]
ENST00000358301; ENSP00000351049; ENSG00000130669
ENST00000360442; ENSP00000353625; ENSG00000130669
ENST00000593690; ENSP00000469413; ENSG00000130669
ENST00000599386; ENSP00000471157; ENSG00000130669 [O96013-3]
ENST00000599470; ENSP00000470284; ENSG00000130669 [O96013-3]
GeneIDi10298
KEGGihsa:10298
UCSCiuc002okj.2, human [O96013-1]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		10298
DisGeNETi10298

GeneCards: human genes, protein and diseases

More...GeneCardsi		PAK4
HGNCiHGNC:16059, PAK4
HPAiENSG00000130669, Low tissue specificity
MIMi605451, gene
neXtProtiNX_O96013
OpenTargetsiENSG00000130669
PharmGKBiPA32920
VEuPathDBiHostDB:ENSG00000130669

Human Unidentified Gene-Encoded large proteins database

More...HUGEi		Search...

GenAtlas: human gene database

More...GenAtlasi		Search...

Phylogenomic databases

eggNOGiKOG0578, Eukaryota
GeneTreeiENSGT01040000240717
HOGENOMiCLU_000288_26_6_1
InParanoidiO96013
OMAiVCIATMK
PhylomeDBiO96013
TreeFamiTF105352

Enzyme and pathway databases

BRENDAi2.7.11.1, 2681
PathwayCommonsiO96013
ReactomeiR-HSA-428540, Activation of RAC1
R-HSA-9013148, CDC42 GTPase cycle
R-HSA-9013149, RAC1 GTPase cycle
R-HSA-9013404, RAC2 GTPase cycle
R-HSA-9013406, RHOQ GTPase cycle
R-HSA-9013407, RHOH GTPase cycle
R-HSA-9013408, RHOG GTPase cycle
R-HSA-9013409, RHOJ GTPase cycle
R-HSA-9013420, RHOU GTPase cycle
R-HSA-9013423, RAC3 GTPase cycle
R-HSA-9013424, RHOV GTPase cycle
SignaLinkiO96013
SIGNORiO96013

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		10298, 15 hits in 1082 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		PAK4, human
EvolutionaryTraceiO96013

The Gene Wiki collection of pages on human genes and proteins

More...GeneWikii		PAK4

Database of phenotypes from RNA interference screens in Drosophila and Homo sapiens

More...GenomeRNAii		10298
PharosiO96013, Tchem

Protein Ontology

More...PROi		PR:O96013
RNActiO96013, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSG00000130669, Expressed in type B pancreatic cell and 245 other tissues
ExpressionAtlasiO96013, baseline and differential
GenevisibleiO96013, HS

Family and domain databases

CDDicd01093, CRIB_PAK_like, 1 hit
DisProtiDP01184
Gene3Di3.90.810.10, 1 hit
InterProiView protein in InterPro
IPR000095, CRIB_dom
IPR036936, CRIB_dom_sf
IPR011009, Kinase-like_dom_sf
IPR033923, PAK_BD
IPR000719, Prot_kinase_dom
IPR017441, Protein_kinase_ATP_BS
PfamiView protein in Pfam
PF00786, PBD, 1 hit
PF00069, Pkinase, 1 hit
SMARTiView protein in SMART
SM00285, PBD, 1 hit
SUPFAMiSSF56112, SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50108, CRIB, 1 hit
PS00107, PROTEIN_KINASE_ATP, 1 hit
PS50011, PROTEIN_KINASE_DOM, 1 hit

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiPAK4_HUMAN
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: O96013
Secondary accession number(s): B4DGG6
, Q8N4E1, Q8NCH5, Q8NDE3, Q9BU33, Q9ULS8
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: May 1, 1999
Last modified: February 23, 2022
This is version 220 of the entry and version 1 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Reference proteome

Documents

  1. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  2. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  3. Human entries with genetic variants
    List of human entries with genetic variants
  4. Human variants curated from literature reports
    Index of human variants curated from literature reports
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  7. SIMILARITY comments
    Index of protein domains and families
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