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Protein

Cholesterol 25-hydroxylase

Gene

CH25H

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.By similarity1 Publication

Catalytic activityi

Cholesterol + AH2 + O2 = 25-hydroxycholesterol + A + H2O.By similarity

Cofactori

Fe cationBy similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciMetaCyc:HS06462-MONOMER
BRENDAi1.14.99.38 2681
ReactomeiR-HSA-192105 Synthesis of bile acids and bile salts

Chemistry databases

SwissLipidsiSLP:000001483

Names & Taxonomyi

Protein namesi
Recommended name:
Cholesterol 25-hydroxylase (EC:1.14.99.38)
Alternative name(s):
Cholesterol 25-monooxygenase
Short name:
h25OH
Gene namesi
Name:CH25H
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 10

Organism-specific databases

EuPathDBiHostDB:ENSG00000138135.6
HGNCiHGNC:1907 CH25H
MIMi604551 gene
neXtProtiNX_O95992

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei38 – 58HelicalSequence analysisAdd BLAST21
Transmembranei84 – 104HelicalSequence analysisAdd BLAST21
Transmembranei121 – 141HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi9023
OpenTargetsiENSG00000138135
PharmGKBiPA26443

Polymorphism and mutation databases

BioMutaiCH25H

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002268011 – 272Cholesterol 25-hydroxylaseAdd BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi5N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi163N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO95992
PRIDEiO95992
ProteomicsDBi51168

Expressioni

Gene expression databases

BgeeiENSG00000138135 Expressed in 176 organ(s), highest expression level in substantia nigra
CleanExiHS_CH25H
GenevisibleiO95992 HS

Interactioni

Protein-protein interaction databases

IntActiO95992, 2 interactors
STRINGi9606.ENSP00000360918

Structurei

3D structure databases

ProteinModelPortaliO95992
SMRiO95992
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi142 – 146Histidine box-15
Motifi157 – 161Histidine box-25
Motifi238 – 244Histidine box-37

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0873 Eukaryota
COG3000 LUCA
GeneTreeiENSGT00530000063017
HOGENOMiHOG000015767
HOVERGENiHBG080944
InParanoidiO95992
KOiK10223
OMAiQFNCNFA
OrthoDBiEOG091G0CY4
PhylomeDBiO95992
TreeFamiTF353265

Family and domain databases

InterProiView protein in InterPro
IPR006694 Fatty_acid_hydroxylase
PfamiView protein in Pfam
PF04116 FA_hydroxylase, 1 hit

Sequencei

Sequence statusi: Complete.

O95992-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSCHNCSDPQ VLCSSGQLFL QPLWDHLRSW EALLQSPFFP VIFSITTYVG
60 70 80 90 100
FCLPFVVLDI LCSWVPALRR YKIHPDFSPS AQQLLPCLGQ TLYQHVMFVF
110 120 130 140 150
PVTLLHWARS PALLPHEAPE LLLLLHHILF CLLLFDMEFF VWHLLHHKVP
160 170 180 190 200
WLYRTFHKVH HQNSSSFALA TQYMSVWELF SLGFFDMMNV TLLGCHPLTT
210 220 230 240 250
LTFHVVNIWL SVEDHSGYNF PWSTHRLVPF GWYGGVVHHD LHHSHFNCNF
260 270
APYFTHWDKI LGTLRTASVP AR
Length:272
Mass (Da):31,745
Last modified:May 1, 1999 - v1
Checksum:i0DF46BC21802008B
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_048899133L → P. Corresponds to variant dbSNP:rs17117295Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059212 Genomic DNA Translation: AAC97481.1
AF059214 mRNA Translation: AAC97483.1
AK314865 mRNA Translation: BAG37380.1
AL513533 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW50146.1
BC017843 mRNA Translation: AAH17843.1
BC072430 mRNA Translation: AAH72430.1
CCDSiCCDS7400.1
RefSeqiNP_003947.1, NM_003956.3
UniGeneiHs.47357

Genome annotation databases

EnsembliENST00000371852; ENSP00000360918; ENSG00000138135
GeneIDi9023
KEGGihsa:9023
UCSCiuc001kfz.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059212 Genomic DNA Translation: AAC97481.1
AF059214 mRNA Translation: AAC97483.1
AK314865 mRNA Translation: BAG37380.1
AL513533 Genomic DNA No translation available.
CH471066 Genomic DNA Translation: EAW50146.1
BC017843 mRNA Translation: AAH17843.1
BC072430 mRNA Translation: AAH72430.1
CCDSiCCDS7400.1
RefSeqiNP_003947.1, NM_003956.3
UniGeneiHs.47357

3D structure databases

ProteinModelPortaliO95992
SMRiO95992
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO95992, 2 interactors
STRINGi9606.ENSP00000360918

Chemistry databases

SwissLipidsiSLP:000001483

Polymorphism and mutation databases

BioMutaiCH25H

Proteomic databases

PaxDbiO95992
PRIDEiO95992
ProteomicsDBi51168

Protocols and materials databases

DNASUi9023
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000371852; ENSP00000360918; ENSG00000138135
GeneIDi9023
KEGGihsa:9023
UCSCiuc001kfz.4 human

Organism-specific databases

CTDi9023
DisGeNETi9023
EuPathDBiHostDB:ENSG00000138135.6
GeneCardsiCH25H
HGNCiHGNC:1907 CH25H
MIMi604551 gene
neXtProtiNX_O95992
OpenTargetsiENSG00000138135
PharmGKBiPA26443
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0873 Eukaryota
COG3000 LUCA
GeneTreeiENSGT00530000063017
HOGENOMiHOG000015767
HOVERGENiHBG080944
InParanoidiO95992
KOiK10223
OMAiQFNCNFA
OrthoDBiEOG091G0CY4
PhylomeDBiO95992
TreeFamiTF353265

Enzyme and pathway databases

BioCyciMetaCyc:HS06462-MONOMER
BRENDAi1.14.99.38 2681
ReactomeiR-HSA-192105 Synthesis of bile acids and bile salts

Miscellaneous databases

GenomeRNAii9023
PROiPR:O95992
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000138135 Expressed in 176 organ(s), highest expression level in substantia nigra
CleanExiHS_CH25H
GenevisibleiO95992 HS

Family and domain databases

InterProiView protein in InterPro
IPR006694 Fatty_acid_hydroxylase
PfamiView protein in Pfam
PF04116 FA_hydroxylase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiCH25H_HUMAN
AccessioniPrimary (citable) accession number: O95992
Secondary accession number(s): B2RBY3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: May 1, 1999
Last modified: October 10, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
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Main funding by: National Institutes of Health

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