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Protein

Reversion-inducing cysteine-rich protein with Kazal motifs

Gene

RECK

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28289266, PubMed:30026314). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (By similarity). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (PubMed:30026314). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (PubMed:30026314). Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed:9789069, PubMed:18194466). Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed:9789069).By similarity4 Publications

GO - Molecular functioni

  • endopeptidase inhibitor activity Source: MGI
  • metalloendopeptidase inhibitor activity Source: ProtInc
  • serine-type endopeptidase inhibitor activity Source: UniProtKB-KW

GO - Biological processi

Keywordsi

Molecular functionProtease inhibitor, Serine protease inhibitor
Biological processWnt signaling pathway

Enzyme and pathway databases

ReactomeiR-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins

Protein family/group databases

MEROPSiI01.037

Names & Taxonomyi

Protein namesi
Recommended name:
Reversion-inducing cysteine-rich protein with Kazal motifs1 Publication
Short name:
hRECK1 Publication
Alternative name(s):
Suppressor of tumorigenicity 15 proteinImported
Gene namesi
Name:RECK1 PublicationImported
Synonyms:ST15Imported
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000122707.11
HGNCiHGNC:11345 RECK
MIMi605227 gene
neXtProtiNX_O95980

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi225 – 272Missing : Abolished interaction with WNT7A. 1 PublicationAdd BLAST48

Keywords - Diseasei

Tumor suppressor

Organism-specific databases

DisGeNETi8434
OpenTargetsiENSG00000122707
PharmGKBiPA34314

Polymorphism and mutation databases

BioMutaiRECK

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Sequence analysisAdd BLAST22
ChainiPRO_000001658323 – 942Reversion-inducing cysteine-rich protein with Kazal motifsAdd BLAST920
PropeptideiPRO_0000016584943 – 971Removed in mature formSequence analysisAdd BLAST29

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi39N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi86N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi200N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi297N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi352N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi633 ↔ 658PROSITE-ProRule annotation
Disulfide bondi635 ↔ 654PROSITE-ProRule annotation
Disulfide bondi643 ↔ 671PROSITE-ProRule annotation
Disulfide bondi716 ↔ 735PROSITE-ProRule annotation
Disulfide bondi724 ↔ 750PROSITE-ProRule annotation
Disulfide bondi761 ↔ 787PROSITE-ProRule annotation
Lipidationi942GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

MaxQBiO95980
PaxDbiO95980
PeptideAtlasiO95980
PRIDEiO95980
ProteomicsDBi51158

PTM databases

iPTMnetiO95980
PhosphoSitePlusiO95980

Expressioni

Tissue specificityi

Expressed in various tissues and untransformed cells (PubMed:9789069). It is undetectable in tumor-derived cell lines and oncogenically transformed cells (PubMed:9789069).1 Publication

Gene expression databases

BgeeiENSG00000122707 Expressed in 223 organ(s), highest expression level in parietal pleura
CleanExiHS_RECK
GenevisibleiO95980 HS

Organism-specific databases

HPAiCAB025109
HPA071729

Interactioni

Subunit structurei

Interacts (via knot repeats) with WNT7A (via disordered linker region); the interaction is direct (PubMed:30026314). Interacts (via knot repeats) with WNT7B (via disordered linker region); the interaction is direct (PubMed:30026314). Interacts with ADGRA2; the interaction is direct (PubMed:30026314). Interacts with MMP9 (PubMed:9789069).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ERBB2P046264EBI-2823742,EBI-641062

Protein-protein interaction databases

BioGridi114014, 17 interactors
IntActiO95980, 4 interactors
MINTiO95980
STRINGi9606.ENSP00000367202

Structurei

3D structure databases

ProteinModelPortaliO95980
SMRiO95980
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati37 – 84Knot 1Add BLAST48
Repeati104 – 141Knot 2Add BLAST38
Repeati151 – 197Knot 3Add BLAST47
Repeati216 – 263Knot 4Add BLAST48
Repeati292 – 338Knot 5Add BLAST47
Domaini627 – 673Kazal-like 1PROSITE-ProRule annotationAdd BLAST47
Domaini698 – 752Kazal-like 2PROSITE-ProRule annotationAdd BLAST55
Domaini753 – 789Kazal-like 3PROSITE-ProRule annotationAdd BLAST37

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni37 – 3385 X Knot repeatsAdd BLAST302

Domaini

The Kazal-like domains mediate the serine protease inhibitor activity.1 Publication

Sequence similaritiesi

Belongs to the RECK family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiKOG3649 Eukaryota
ENOG410YC3T LUCA
GeneTreeiENSGT00390000018540
HOGENOMiHOG000007167
HOVERGENiHBG036100
InParanoidiO95980
KOiK17461
OMAiISKDPCN
OrthoDBiEOG091G017H
PhylomeDBiO95980
TreeFamiTF324424

Family and domain databases

InterProiView protein in InterPro
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR039016 RECK
PANTHERiPTHR13487 PTHR13487, 1 hit
PfamiView protein in Pfam
PF07648 Kazal_2, 3 hits
SMARTiView protein in SMART
SM00280 KAZAL, 3 hits
SUPFAMiSSF100895 SSF100895, 3 hits
PROSITEiView protein in PROSITE
PS00282 KAZAL_1, 1 hit
PS51465 KAZAL_2, 3 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O95980-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATVRASLRG ALLLLLAVAG VAEVAGGLAP GSAGALCCNH SKDNQMCRDV
60 70 80 90 100
CEQIFSSKSE SRLKHLLQRA PDYCPETMVE IWNCMNSSLP GVFKKSDGWV
110 120 130 140 150
GLGCCELAIA LECRQACKQA SSKNDISKVC RKEYENALFS CISRNEMGSV
160 170 180 190 200
CCSYAGHHTN CREYCQAIFR TDSSPGPSQI KAVENYCASI SPQLIHCVNN
210 220 230 240 250
YTQSYPMRNP TDSLYCCDRA EDHACQNACK RILMSKKTEM EIVDGLIEGC
260 270 280 290 300
KTQPLPQDPL WQCFLESSQS VHPGVTVHPP PSTGLDGAKL HCCSKANTST
310 320 330 340 350
CRELCTKLYS MSWGNTQSWQ EFDRFCEYNP VEVSMLTCLA DVREPCQLGC
360 370 380 390 400
RNLTYCTNFN NRPTELFRSC NAQSDQGAMN DMKLWEKGSI KMPFINIPVL
410 420 430 440 450
DIKKCQPEMW KAIACSLQIK PCHSKSRGSI ICKSDCVEIL KKCGDQNKFP
460 470 480 490 500
EDHTAESICE LLSPTDDLKN CIPLDTYLRP STLGNIVEEV THPCNPNPCP
510 520 530 540 550
ANELCEVNRK GCPSGDPCLP YFCVQGCKLG EASDFIVRQG TLIQVPSSAG
560 570 580 590 600
EVGCYKICSC GQSGLLENCM EMHCIDLQKS CIVGGKRKSH GTSFSIDCNV
610 620 630 640 650
CSCFAGNLVC STRLCLSEHS SEDDRRTFTG LPCNCADQFV PVCGQNGRTY
660 670 680 690 700
PSACIARCVG LQDHQFEFGS CMSKDPCNPN PCQKNQRCIP KPQVCLTTFD
710 720 730 740 750
KFGCSQYECV PRQLACDQVQ DPVCDTDHME HNNLCTLYQR GKSLSYKGPC
760 770 780 790 800
QPFCRATEPV CGHNGETYSS VCAAYSDRVA VDYYGDCQAV GVLSEHSSVA
810 820 830 840 850
ECASVKCPSL LAAGCKPIIP PGACCPLCAG MLRVLFDKEK LDTIAKVTNK
860 870 880 890 900
KPITVLEILQ KIRMHVSVPQ CDVFGYFSIE SEIVILIIPV DHYPKALQIE
910 920 930 940 950
ACNKEAEKIE SLINSDSPTL ASHVPLSALI ISQVQVSSSV PSAGVRARPS
960 970
CHSLLLPLSL GLALHLLWTY N
Length:971
Mass (Da):106,457
Last modified:May 1, 1999 - v1
Checksum:i173D47D6AEE6F834
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_034021275V → I. Corresponds to variant dbSNP:rs16932912Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50406 mRNA Translation: BAA34060.1
AL158830 Genomic DNA No translation available.
AL138834 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58318.1
BC137093 mRNA Translation: AAI37094.1
CCDSiCCDS6597.1
RefSeqiNP_066934.1, NM_021111.2
UniGeneiHs.388918

Genome annotation databases

EnsembliENST00000377966; ENSP00000367202; ENSG00000122707
GeneIDi8434
KEGGihsa:8434
UCSCiuc003zyv.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50406 mRNA Translation: BAA34060.1
AL158830 Genomic DNA No translation available.
AL138834 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58318.1
BC137093 mRNA Translation: AAI37094.1
CCDSiCCDS6597.1
RefSeqiNP_066934.1, NM_021111.2
UniGeneiHs.388918

3D structure databases

ProteinModelPortaliO95980
SMRiO95980
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114014, 17 interactors
IntActiO95980, 4 interactors
MINTiO95980
STRINGi9606.ENSP00000367202

Protein family/group databases

MEROPSiI01.037

PTM databases

iPTMnetiO95980
PhosphoSitePlusiO95980

Polymorphism and mutation databases

BioMutaiRECK

Proteomic databases

MaxQBiO95980
PaxDbiO95980
PeptideAtlasiO95980
PRIDEiO95980
ProteomicsDBi51158

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000377966; ENSP00000367202; ENSG00000122707
GeneIDi8434
KEGGihsa:8434
UCSCiuc003zyv.4 human

Organism-specific databases

CTDi8434
DisGeNETi8434
EuPathDBiHostDB:ENSG00000122707.11
GeneCardsiRECK
HGNCiHGNC:11345 RECK
HPAiCAB025109
HPA071729
MIMi605227 gene
neXtProtiNX_O95980
OpenTargetsiENSG00000122707
PharmGKBiPA34314
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3649 Eukaryota
ENOG410YC3T LUCA
GeneTreeiENSGT00390000018540
HOGENOMiHOG000007167
HOVERGENiHBG036100
InParanoidiO95980
KOiK17461
OMAiISKDPCN
OrthoDBiEOG091G017H
PhylomeDBiO95980
TreeFamiTF324424

Enzyme and pathway databases

ReactomeiR-HSA-163125 Post-translational modification: synthesis of GPI-anchored proteins

Miscellaneous databases

ChiTaRSiRECK human
GeneWikiiRECK
GenomeRNAii8434
PROiPR:O95980
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000122707 Expressed in 223 organ(s), highest expression level in parietal pleura
CleanExiHS_RECK
GenevisibleiO95980 HS

Family and domain databases

InterProiView protein in InterPro
IPR002350 Kazal_dom
IPR036058 Kazal_dom_sf
IPR039016 RECK
PANTHERiPTHR13487 PTHR13487, 1 hit
PfamiView protein in Pfam
PF07648 Kazal_2, 3 hits
SMARTiView protein in SMART
SM00280 KAZAL, 3 hits
SUPFAMiSSF100895 SSF100895, 3 hits
PROSITEiView protein in PROSITE
PS00282 KAZAL_1, 1 hit
PS51465 KAZAL_2, 3 hits
ProtoNetiSearch...

Entry informationi

Entry nameiRECK_HUMAN
AccessioniPrimary (citable) accession number: O95980
Secondary accession number(s): B2RNS1, Q5W0K6, Q8WX37
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: May 1, 1999
Last modified: November 7, 2018
This is version 148 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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