Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitochondrial mRNA pseudouridine synthase TRUB2

Gene

TRUB2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Minor enzyme contributing to the isomerization of uridine to pseudouridine (pseudouridylation) of specific mitochondrial mRNAs (mt-mRNAs) such as COXI and COXIII mt-mRNAs (PubMed:27974379). As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and is required for intra-mitochondrial translation (PubMed:27667664).2 Publications

Catalytic activityi

mRNA uridine = mRNA pseudouridine.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei98NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • mRNA processing Source: UniProtKB-KW
  • positive regulation of mitochondrial translation Source: UniProtKB
  • pseudouridine synthesis Source: InterPro

Keywordsi

Molecular functionIsomerase
Biological processmRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial mRNA pseudouridine synthase TRUB2 (EC:5.4.99.-1 Publication)
Gene namesi
Name:TRUB2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000167112.9
HGNCiHGNC:17170 TRUB2
MIMi610727 gene
neXtProtiNX_O95900

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000167112
PharmGKBiPA38209

Polymorphism and mutation databases

BioMutaiTRUB2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 10MitochondrionSequence analysis10
ChainiPRO_000025209011 – 331Mitochondrial mRNA pseudouridine synthase TRUB2Add BLAST321

Proteomic databases

EPDiO95900
MaxQBiO95900
PaxDbiO95900
PeptideAtlasiO95900
PRIDEiO95900
ProteomicsDBi51120

PTM databases

iPTMnetiO95900
PhosphoSitePlusiO95900

Expressioni

Gene expression databases

BgeeiENSG00000167112
CleanExiHS_TRUB2
ExpressionAtlasiO95900 baseline and differential
GenevisibleiO95900 HS

Organism-specific databases

HPAiHPA021211
HPA052190

Interactioni

Subunit structurei

Forms a regulatory protein-RNA complex, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mt-rRNA.1 Publication

Protein-protein interaction databases

BioGridi117942, 56 interactors
IntActiO95900, 9 interactors
MINTiO95900
STRINGi9606.ENSP00000361982

Structurei

3D structure databases

ProteinModelPortaliO95900
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2559 Eukaryota
ENOG4110V1W LUCA
GeneTreeiENSGT00530000063611
HOGENOMiHOG000231821
HOVERGENiHBG053337
InParanoidiO95900
OMAiLIRPMNK
OrthoDBiEOG091G0FSQ
PhylomeDBiO95900
TreeFamiTF320759

Family and domain databases

InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR002501 PsdUridine_synth_N
IPR039048 Trub2
PANTHERiPTHR13195 PTHR13195, 1 hit
PfamiView protein in Pfam
PF01509 TruB_N, 1 hit
SUPFAMiSSF55120 SSF55120, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O95900-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSAGLSRLH GLFAVYKPPG LKWKHLRDTV ELQLLKGLNA RKPPAPKQRV
60 70 80 90 100
RFLLGPMEGS EEKELTLTAT SVPSFINHPL VCGPAFAHLK VGVGHRLDAQ
110 120 130 140 150
ASGVLVLGVG HGCRLLTDMY NAHLTKDYTV RGLLGKATDD FREDGRLVEK
160 170 180 190 200
TTYDHVTREK LDRILAVIQG SHQKALVMYS NLDLKTQEAY EMAVRGLIRP
210 220 230 240 250
MNKSPMLITG IRCLYFAPPE FLLEVQCMHE TQKELRKLVH EIGLELKTTA
260 270 280 290 300
VCTQVRRTRD GFFTLDSALL RTQWDLTNIQ DAIRAATPQV AAELEKSLSP
310 320 330
GLDTKQLPSP GWSWDSQGPS STLGLERGAG Q
Length:331
Mass (Da):36,694
Last modified:May 1, 1999 - v1
Checksum:i60DD5F9FEA998684
GO
Isoform 2 (identifier: O95900-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-56: Missing.

Note: No experimental confirmation available.
Show »
Length:275
Mass (Da):30,454
Checksum:iF13BE676EC85FACB
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05160879P → L. Corresponds to variant dbSNP:rs2231630Ensembl.1
Natural variantiVAR_02774993V → L. Corresponds to variant dbSNP:rs2072394Ensembl.1
Natural variantiVAR_051609209T → S. Corresponds to variant dbSNP:rs2231637Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0560941 – 56Missing in isoform 2. 1 PublicationAdd BLAST56

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF131848 mRNA Translation: AAD20059.1
AK001956 mRNA Translation: BAA92001.1
AK301986 mRNA Translation: BAH13601.1
AL359091 Genomic DNA No translation available.
BC001457 mRNA Translation: AAH01457.1
CCDSiCCDS6897.1 [O95900-1]
RefSeqiNP_001316790.1, NM_001329861.1
NP_001316791.1, NM_001329862.1 [O95900-2]
NP_001316792.1, NM_001329863.1
NP_056494.1, NM_015679.2 [O95900-1]
UniGeneiHs.632685

Genome annotation databases

EnsembliENST00000372890; ENSP00000361982; ENSG00000167112 [O95900-1]
GeneIDi26995
KEGGihsa:26995
UCSCiuc004buq.2 human [O95900-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Entry informationi

Entry nameiTRUB2_HUMAN
AccessioniPrimary (citable) accession number: O95900
Secondary accession number(s): B7Z7G5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 3, 2006
Last sequence update: May 1, 1999
Last modified: July 18, 2018
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health