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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 2

Gene

DDAH2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei171Proton donorBy similarity1
Active sitei276NucleophileSequence analysis1

GO - Molecular functioni

  • amino acid binding Source: GO_Central
  • catalytic activity Source: ProtInc
  • dimethylargininase activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase

Enzyme and pathway databases

BRENDAi3.5.3.18 2681
ReactomeiR-HSA-203615 eNOS activation

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 (EC:3.5.3.18)
Short name:
DDAH-2
Short name:
Dimethylarginine dimethylaminohydrolase 2
Alternative name(s):
DDAHII
Dimethylargininase-2
Protein G6a
S-phase protein
Gene namesi
Name:DDAH2
Synonyms:DDAH, G6A, NG30
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

EuPathDBiHostDB:ENSG00000213722.8
HGNCiHGNC:2716 DDAH2
MIMi604744 gene
neXtProtiNX_O95865

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Organism-specific databases

DisGeNETi23564
OpenTargetsiENSG00000213722
PharmGKBiPA27186

Chemistry databases

DrugBankiDB00155 L-Citrulline

Polymorphism and mutation databases

BioMutaiDDAH2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001711211 – 285N(G),N(G)-dimethylarginine dimethylaminohydrolase 2Add BLAST285

Proteomic databases

EPDiO95865
PaxDbiO95865
PeptideAtlasiO95865
PRIDEiO95865
ProteomicsDBi51100

2D gel databases

REPRODUCTION-2DPAGEiIPI00000760
O95865

PTM databases

iPTMnetiO95865
PhosphoSitePlusiO95865

Expressioni

Tissue specificityi

Detected in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas, and at very low levels in brain.1 Publication

Gene expression databases

BgeeiENSG00000213722 Expressed in 217 organ(s), highest expression level in endocervix
CleanExiHS_DDAH2
ExpressionAtlasiO95865 baseline and differential
GenevisibleiO95865 HS

Organism-specific databases

HPAiHPA012509

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TRIP13Q156455EBI-749139,EBI-358993

Protein-protein interaction databases

BioGridi117107, 37 interactors
IntActiO95865, 26 interactors
MINTiO95865
STRINGi9606.ENSP00000364943

Structurei

3D structure databases

ProteinModelPortaliO95865
SMRiO95865
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiENOG410ISNS Eukaryota
ENOG4111YMV LUCA
GeneTreeiENSGT00390000009331
HOGENOMiHOG000161035
HOVERGENiHBG055937
InParanoidiO95865
KOiK01482
OMAiAQRQFGC
OrthoDBiEOG0937146R
PhylomeDBiO95865
TreeFamiTF314737

Family and domain databases

InterProiView protein in InterPro
IPR033199 DDAH/AD
IPR033202 DDAH2
PANTHERiPTHR12737 PTHR12737, 1 hit
PTHR12737:SF16 PTHR12737:SF16, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

O95865-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGTPGEGLGR CSHALIRGVP ESLASGEGAG AGLPALDLAK AQREHGVLGG
60 70 80 90 100
KLRQRLGLQL LELPPEESLP LGPLLGDTAV IQGDTALITR PWSPARRPEV
110 120 130 140 150
DGVRKALQDL GLRIVEIGDE NATLDGTDVL FTGREFFVGL SKWTNHRGAE
160 170 180 190 200
IVADTFRDFA VSTVPVSGPS HLRGLCGMGG PRTVVAGSSD AAQKAVRAMA
210 220 230 240 250
VLTDHPYASL TLPDDAAADC LFLRPGLPGV PPFLLHRGGG DLPNSQEALQ
260 270 280
KLSDVTLVPV SCSELEKAGA GLSSLCLVLS TRPHS
Length:285
Mass (Da):29,644
Last modified:May 1, 1999 - v1
Checksum:iB3D9B00F29492548
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q5SSV3Q5SSV3_HUMAN
N(G),N(G)-dimethylarginine dimethyl...
DDAH2
226Annotation score:
Q5SRR8Q5SRR8_HUMAN
N(G),N(G)-dimethylarginine dimethyl...
DDAH2
236Annotation score:
H0Y7N1H0Y7N1_HUMAN
N(G),N(G)-dimethylarginine dimethyl...
DDAH2
188Annotation score:
A0A140T971A0A140T971_HUMAN
N(G),N(G)-dimethylarginine dimethyl...
DDAH2
225Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070667 mRNA Translation: AAD20973.1
AJ012008 Genomic DNA Translation: CAB46079.1
AF087894 mRNA Translation: AAP97193.1
AF129756 Genomic DNA Translation: AAD18074.1
BA000025 Genomic DNA Translation: BAB63377.1
AL662899 Genomic DNA No translation available.
AL670886 Genomic DNA No translation available.
AL844216 Genomic DNA No translation available.
BX248244 Genomic DNA No translation available.
CR354443 Genomic DNA No translation available.
CR759787 Genomic DNA No translation available.
CR936239 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03500.1
BC001435 mRNA Translation: AAH01435.1
CCDSiCCDS4718.1
RefSeqiNP_001289936.1, NM_001303007.1
NP_001289937.1, NM_001303008.1
NP_039268.1, NM_013974.2
XP_011512750.1, XM_011514448.2
UniGeneiHs.247362

Genome annotation databases

EnsembliENST00000375787; ENSP00000364943; ENSG00000213722
ENST00000375789; ENSP00000364945; ENSG00000213722
ENST00000375792; ENSP00000364949; ENSG00000213722
ENST00000383409; ENSP00000372901; ENSG00000206395
ENST00000400062; ENSP00000382935; ENSG00000206395
ENST00000400063; ENSP00000382936; ENSG00000206395
ENST00000411456; ENSP00000409396; ENSG00000226634
ENST00000413532; ENSP00000402594; ENSG00000226634
ENST00000413655; ENSP00000412800; ENSG00000233076
ENST00000414455; ENSP00000404342; ENSG00000225635
ENST00000424790; ENSP00000391632; ENSG00000233076
ENST00000426149; ENSP00000412327; ENSG00000228128
ENST00000427126; ENSP00000395372; ENSG00000228128
ENST00000430482; ENSP00000408148; ENSG00000227317
ENST00000434464; ENSP00000391833; ENSG00000228128
ENST00000437889; ENSP00000399023; ENSG00000227317
ENST00000443533; ENSP00000389552; ENSG00000226634
ENST00000444699; ENSP00000404851; ENSG00000225635
ENST00000447101; ENSP00000405515; ENSG00000233076
ENST00000451411; ENSP00000403154; ENSG00000227317
ENST00000454138; ENSP00000399357; ENSG00000225635
GeneIDi23564
KEGGihsa:23564

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF070667 mRNA Translation: AAD20973.1
AJ012008 Genomic DNA Translation: CAB46079.1
AF087894 mRNA Translation: AAP97193.1
AF129756 Genomic DNA Translation: AAD18074.1
BA000025 Genomic DNA Translation: BAB63377.1
AL662899 Genomic DNA No translation available.
AL670886 Genomic DNA No translation available.
AL844216 Genomic DNA No translation available.
BX248244 Genomic DNA No translation available.
CR354443 Genomic DNA No translation available.
CR759787 Genomic DNA No translation available.
CR936239 Genomic DNA No translation available.
CH471081 Genomic DNA Translation: EAX03500.1
BC001435 mRNA Translation: AAH01435.1
CCDSiCCDS4718.1
RefSeqiNP_001289936.1, NM_001303007.1
NP_001289937.1, NM_001303008.1
NP_039268.1, NM_013974.2
XP_011512750.1, XM_011514448.2
UniGeneiHs.247362

3D structure databases

ProteinModelPortaliO95865
SMRiO95865
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117107, 37 interactors
IntActiO95865, 26 interactors
MINTiO95865
STRINGi9606.ENSP00000364943

Chemistry databases

DrugBankiDB00155 L-Citrulline

PTM databases

iPTMnetiO95865
PhosphoSitePlusiO95865

Polymorphism and mutation databases

BioMutaiDDAH2

2D gel databases

REPRODUCTION-2DPAGEiIPI00000760
O95865

Proteomic databases

EPDiO95865
PaxDbiO95865
PeptideAtlasiO95865
PRIDEiO95865
ProteomicsDBi51100

Protocols and materials databases

DNASUi23564
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000375787; ENSP00000364943; ENSG00000213722
ENST00000375789; ENSP00000364945; ENSG00000213722
ENST00000375792; ENSP00000364949; ENSG00000213722
ENST00000383409; ENSP00000372901; ENSG00000206395
ENST00000400062; ENSP00000382935; ENSG00000206395
ENST00000400063; ENSP00000382936; ENSG00000206395
ENST00000411456; ENSP00000409396; ENSG00000226634
ENST00000413532; ENSP00000402594; ENSG00000226634
ENST00000413655; ENSP00000412800; ENSG00000233076
ENST00000414455; ENSP00000404342; ENSG00000225635
ENST00000424790; ENSP00000391632; ENSG00000233076
ENST00000426149; ENSP00000412327; ENSG00000228128
ENST00000427126; ENSP00000395372; ENSG00000228128
ENST00000430482; ENSP00000408148; ENSG00000227317
ENST00000434464; ENSP00000391833; ENSG00000228128
ENST00000437889; ENSP00000399023; ENSG00000227317
ENST00000443533; ENSP00000389552; ENSG00000226634
ENST00000444699; ENSP00000404851; ENSG00000225635
ENST00000447101; ENSP00000405515; ENSG00000233076
ENST00000451411; ENSP00000403154; ENSG00000227317
ENST00000454138; ENSP00000399357; ENSG00000225635
GeneIDi23564
KEGGihsa:23564

Organism-specific databases

CTDi23564
DisGeNETi23564
EuPathDBiHostDB:ENSG00000213722.8
GeneCardsiDDAH2
HGNCiHGNC:2716 DDAH2
HPAiHPA012509
MIMi604744 gene
neXtProtiNX_O95865
OpenTargetsiENSG00000213722
PharmGKBiPA27186
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410ISNS Eukaryota
ENOG4111YMV LUCA
GeneTreeiENSGT00390000009331
HOGENOMiHOG000161035
HOVERGENiHBG055937
InParanoidiO95865
KOiK01482
OMAiAQRQFGC
OrthoDBiEOG0937146R
PhylomeDBiO95865
TreeFamiTF314737

Enzyme and pathway databases

BRENDAi3.5.3.18 2681
ReactomeiR-HSA-203615 eNOS activation

Miscellaneous databases

ChiTaRSiDDAH2 human
GenomeRNAii23564
PROiPR:O95865
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000213722 Expressed in 217 organ(s), highest expression level in endocervix
CleanExiHS_DDAH2
ExpressionAtlasiO95865 baseline and differential
GenevisibleiO95865 HS

Family and domain databases

InterProiView protein in InterPro
IPR033199 DDAH/AD
IPR033202 DDAH2
PANTHERiPTHR12737 PTHR12737, 1 hit
PTHR12737:SF16 PTHR12737:SF16, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDDAH2_HUMAN
AccessioniPrimary (citable) accession number: O95865
Secondary accession number(s): A2BEZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 7, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
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Main funding by: National Institutes of Health

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