UniProtKB - O95832 (CLD1_HUMAN)
Protein
Claudin-1
Gene
CLDN1
Organism
Homo sapiens (Human)
Status
Functioni
Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions (PubMed:23407391).1 Publication
(Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes (PubMed:17325668, PubMed:20375010, PubMed:24038151). Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell (PubMed:20375010). Acts as a receptor for dengue virus (PubMed:24074594).4 Publications
GO - Molecular functioni
- identical protein binding Source: UniProtKB
- structural molecule activity Source: InterPro
- virus receptor activity Source: UniProtKB
GO - Biological processi
- aging Source: Ensembl
- bicellular tight junction assembly Source: UniProtKB
- calcium-independent cell-cell adhesion via plasma membrane cell-adhesion molecules Source: UniProtKB
- cell adhesion Source: GO_Central
- cell-cell junction organization Source: MGI
- cell junction maintenance Source: ARUK-UCL
- cellular response to butyrate Source: Ensembl
- cellular response to interferon-gamma Source: Ensembl
- cellular response to lead ion Source: Ensembl
- cellular response to transforming growth factor beta stimulus Source: Ensembl
- cellular response to tumor necrosis factor Source: Ensembl
- establishment of blood-nerve barrier Source: Ensembl
- establishment of endothelial intestinal barrier Source: Ensembl
- establishment of skin barrier Source: UniProtKB
- hyperosmotic salinity response Source: Ensembl
- liver regeneration Source: Ensembl
- maintenance of blood-brain barrier Source: ARUK-UCL
- positive regulation of bicellular tight junction assembly Source: Ensembl
- positive regulation of cell migration Source: ARUK-UCL
- positive regulation of epithelial cell proliferation involved in wound healing Source: ARUK-UCL
- positive regulation of wound healing Source: ARUK-UCL
- protein complex oligomerization Source: ARUK-UCL
- response to dexamethasone Source: Ensembl
- response to ethanol Source: Ensembl
- response to interleukin-18 Source: Ensembl
- response to lipopolysaccharide Source: Ensembl
- response to toxic substance Source: Ensembl
- xenobiotic transport across blood-nerve barrier Source: Ensembl
Keywordsi
Molecular function | Host cell receptor for virus entry, Receptor |
Biological process | Host-virus interaction |
Enzyme and pathway databases
PathwayCommonsi | O95832 |
Reactomei | R-HSA-420029, Tight junction interactions |
Protein family/group databases
TCDBi | 1.H.1.1.14, the claudin tight junction (claudin1) family |
Names & Taxonomyi
Protein namesi | Recommended name: Claudin-1Alternative name(s): Senescence-associated epithelial membrane protein |
Gene namesi | Name:CLDN1 Synonyms:CLD1, SEMP1 ORF Names:UNQ481/PRO944 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:2032, CLDN1 |
MIMi | 603718, gene |
neXtProti | NX_O95832 |
VEuPathDBi | HostDB:ENSG00000163347.5 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication; Multi-pass membrane protein Sequence analysis
- Basolateral cell membrane 1 Publication
Other locations
- tight junction 2 Publications
Note: Associates with CD81 and the CLDN1-CD81 complex localizes to the basolateral cell membrane.1 Publication
Plasma Membrane
- apical plasma membrane Source: Ensembl
- basolateral plasma membrane Source: UniProtKB
- integral component of plasma membrane Source: UniProtKB
- lateral plasma membrane Source: Ensembl
- plasma membrane Source: ARUK-UCL
Other locations
- bicellular tight junction Source: UniProtKB
- cytoplasm Source: Ensembl
- integral component of membrane Source: ProtInc
- protein-containing complex Source: UniProtKB
- tight junction Source: ARUK-UCL
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 7 | CytoplasmicBy similarity | 7 | |
Transmembranei | 8 – 28 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 29 – 81 | ExtracellularSequence analysisAdd BLAST | 53 | |
Transmembranei | 82 – 102 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 103 – 115 | CytoplasmicSequence analysisAdd BLAST | 13 | |
Transmembranei | 116 – 136 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 137 – 163 | ExtracellularSequence analysisAdd BLAST | 27 | |
Transmembranei | 164 – 184 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 185 – 211 | CytoplasmicBy similarityAdd BLAST | 27 |
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Tight junctionPathology & Biotechi
Involvement in diseasei
Ichthyosis-sclerosing cholangitis neonatal syndrome (NISCH)2 Publications
The disease is caused by variants affecting the gene represented in this entry.
Disease descriptionA rare autosomal recessive complex ichthyosis syndrome characterized by scalp hypotrichosis, scarring alopecia, mild diffuse ichthyosis, sclerosing cholangitis and leukocyte vacuolization.
Related information in OMIMMutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 32 | I → M: Loss of HCV receptor activity. Significant loss of interaction with CD81. Reduced interaction with OCLN. 2 Publications | 1 | |
Mutagenesisi | 48 | E → K: Loss of HCV receptor activity. Significant loss of interaction with CD81. Reduced interaction with OCLN. According to PubMed:17325668 no effect observed on HCV infection susceptibility in cell culture. 2 Publications | 1 |
Keywords - Diseasei
IchthyosisOrganism-specific databases
DisGeNETi | 9076 |
MalaCardsi | CLDN1 |
MIMi | 607626, phenotype |
OpenTargetsi | ENSG00000163347 |
Orphaneti | 59303, Neonatal ichthyosis-sclerosing cholangitis syndrome |
PharmGKBi | PA26557 |
Miscellaneous databases
Pharosi | O95832, Tbio |
Genetic variation databases
BioMutai | CLDN1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000144729 | 1 – 211 | Claudin-1Add BLAST | 211 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 54 ↔ 64 | By similarity |
Keywords - PTMi
Disulfide bondProteomic databases
EPDi | O95832 |
jPOSTi | O95832 |
MassIVEi | O95832 |
MaxQBi | O95832 |
PaxDbi | O95832 |
PeptideAtlasi | O95832 |
PRIDEi | O95832 |
ProteomicsDBi | 51077 |
PTM databases
iPTMneti | O95832 |
PhosphoSitePlusi | O95832 |
SwissPalmi | O95832 |
Expressioni
Tissue specificityi
Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.1 Publication
Gene expression databases
Bgeei | ENSG00000163347, Expressed in skin of abdomen and 173 other tissues |
ExpressionAtlasi | O95832, baseline and differential |
Genevisiblei | O95832, HS |
Organism-specific databases
HPAi | ENSG00000163347, Tissue enhanced (liver, skin) |
Interactioni
Subunit structurei
Homopolymers interact with CLDN3, but not CLDN2, homopolymers. Can form homo- and heteropolymers with other claudin family members (PubMed:23704991). Directly interacts with TJP1/ZO-1, TJP2/ZO-2 and TJP3/ZO-3.
Interacts with MPDZ and PATJ (By similarity).
Interacts with OCLN, CLDN4, CLDN6 and CLDN9 (PubMed:20375010).
Interacts with CD81 (PubMed:20375010, PubMed:21516087).
By similarity3 Publications(Microbial infection) Interacts with hepatitis c virus heterodimer E1/E2.
1 Publication(Microbial infection) Interacts with dengue virus small envelope protein M.
1 PublicationBinary interactionsi
Hide detailsO95832
GO - Molecular functioni
- identical protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 114533, 18 interactors |
CORUMi | O95832 |
IntActi | O95832, 11 interactors |
MINTi | O95832 |
STRINGi | 9606.ENSP00000295522 |
Miscellaneous databases
RNActi | O95832, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 210 – 211 | Interactions with TJP1, TJP2, TJP3 and PATJBy similarity | 2 |
Sequence similaritiesi
Belongs to the claudin family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502R7HF, Eukaryota |
GeneTreei | ENSGT00940000155387 |
HOGENOMi | CLU_076370_2_3_1 |
InParanoidi | O95832 |
OMAi | IGMKCMK |
OrthoDBi | 1244077at2759 |
PhylomeDBi | O95832 |
TreeFami | TF331936 |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR003548, Claudin1 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit PTHR12002:SF92, PTHR12002:SF92, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01377, CLAUDIN1 |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
i Sequence
Sequence statusi: Complete.
O95832-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MANAGLQLLG FILAFLGWIG AIVSTALPQW RIYSYAGDNI VTAQAMYEGL
60 70 80 90 100
WMSCVSQSTG QIQCKVFDSL LNLSSTLQAT RALMVVGILL GVIAIFVATV
110 120 130 140 150
GMKCMKCLED DEVQKMRMAV IGGAIFLLAG LAILVATAWY GNRIVQEFYD
160 170 180 190 200
PMTPVNARYE FGQALFTGWA AASLCLLGGA LLCCSCPRKT TSYPTPRPYP
210
KPAPSSGKDY V
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 62 | I → V in AAD22962 (Ref. 2) Curated | 1 | |
Sequence conflicti | 135 | V → A in AAD22962 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF101051 mRNA Translation: AAD16433.1 AF115546 mRNA Translation: AAD22962.1 AF134160 mRNA Translation: AAF61393.1 AF260406 , AF260403, AF260404, AF260405 Genomic DNA Translation: AAK20945.1 AY358652 mRNA Translation: AAQ89015.1 CR457138 mRNA Translation: CAG33419.1 BC012471 mRNA Translation: AAH12471.1 |
CCDSi | CCDS3295.1 |
RefSeqi | NP_066924.1, NM_021101.4 |
Genome annotation databases
Ensembli | ENST00000295522; ENSP00000295522; ENSG00000163347 |
GeneIDi | 9076 |
KEGGi | hsa:9076 |
UCSCi | uc003fsh.4, human |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF101051 mRNA Translation: AAD16433.1 AF115546 mRNA Translation: AAD22962.1 AF134160 mRNA Translation: AAF61393.1 AF260406 , AF260403, AF260404, AF260405 Genomic DNA Translation: AAK20945.1 AY358652 mRNA Translation: AAQ89015.1 CR457138 mRNA Translation: CAG33419.1 BC012471 mRNA Translation: AAH12471.1 |
CCDSi | CCDS3295.1 |
RefSeqi | NP_066924.1, NM_021101.4 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
BioGRIDi | 114533, 18 interactors |
CORUMi | O95832 |
IntActi | O95832, 11 interactors |
MINTi | O95832 |
STRINGi | 9606.ENSP00000295522 |
Protein family/group databases
TCDBi | 1.H.1.1.14, the claudin tight junction (claudin1) family |
PTM databases
iPTMneti | O95832 |
PhosphoSitePlusi | O95832 |
SwissPalmi | O95832 |
Genetic variation databases
BioMutai | CLDN1 |
Proteomic databases
EPDi | O95832 |
jPOSTi | O95832 |
MassIVEi | O95832 |
MaxQBi | O95832 |
PaxDbi | O95832 |
PeptideAtlasi | O95832 |
PRIDEi | O95832 |
ProteomicsDBi | 51077 |
Protocols and materials databases
Antibodypediai | 3613, 761 antibodies |
DNASUi | 9076 |
Genome annotation databases
Ensembli | ENST00000295522; ENSP00000295522; ENSG00000163347 |
GeneIDi | 9076 |
KEGGi | hsa:9076 |
UCSCi | uc003fsh.4, human |
Organism-specific databases
CTDi | 9076 |
DisGeNETi | 9076 |
GeneCardsi | CLDN1 |
HGNCi | HGNC:2032, CLDN1 |
HPAi | ENSG00000163347, Tissue enhanced (liver, skin) |
MalaCardsi | CLDN1 |
MIMi | 603718, gene 607626, phenotype |
neXtProti | NX_O95832 |
OpenTargetsi | ENSG00000163347 |
Orphaneti | 59303, Neonatal ichthyosis-sclerosing cholangitis syndrome |
PharmGKBi | PA26557 |
VEuPathDBi | HostDB:ENSG00000163347.5 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | ENOG502R7HF, Eukaryota |
GeneTreei | ENSGT00940000155387 |
HOGENOMi | CLU_076370_2_3_1 |
InParanoidi | O95832 |
OMAi | IGMKCMK |
OrthoDBi | 1244077at2759 |
PhylomeDBi | O95832 |
TreeFami | TF331936 |
Enzyme and pathway databases
PathwayCommonsi | O95832 |
Reactomei | R-HSA-420029, Tight junction interactions |
Miscellaneous databases
BioGRID-ORCSi | 9076, 7 hits in 868 CRISPR screens |
ChiTaRSi | CLDN1, human |
GeneWikii | CLDN1 |
GenomeRNAii | 9076 |
Pharosi | O95832, Tbio |
PROi | PR:O95832 |
RNActi | O95832, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000163347, Expressed in skin of abdomen and 173 other tissues |
ExpressionAtlasi | O95832, baseline and differential |
Genevisiblei | O95832, HS |
Family and domain databases
InterProi | View protein in InterPro IPR006187, Claudin IPR003548, Claudin1 IPR017974, Claudin_CS IPR004031, PMP22/EMP/MP20/Claudin |
PANTHERi | PTHR12002, PTHR12002, 1 hit PTHR12002:SF92, PTHR12002:SF92, 1 hit |
Pfami | View protein in Pfam PF00822, PMP22_Claudin, 1 hit |
PRINTSi | PR01377, CLAUDIN1 |
PROSITEi | View protein in PROSITE PS01346, CLAUDIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | CLD1_HUMAN | |
Accessioni | O95832Primary (citable) accession number: O95832 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2000 |
Last sequence update: | May 1, 1999 | |
Last modified: | February 10, 2021 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 3
Human chromosome 3: entries, gene names and cross-references to MIM - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families