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Protein

Probable ATP-dependent RNA helicase DDX58

Gene

DDX58

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Innate immune receptor which acts as a cytoplasmic sensor of viral nucleic acids and plays a major role in sensing viral infection and in the activation of a cascade of antiviral responses including the induction of type I interferons and proinflammatory cytokines. Its ligands include: 5'-triphosphorylated ssRNA and dsRNA and short dsRNA (<1 kb in length). In addition to the 5'-triphosphate moiety, blunt-end base pairing at the 5'-end of the RNA is very essential. Overhangs at the non-triphosphorylated end of the dsRNA RNA have no major impact on its activity. A 3'overhang at the 5'triphosphate end decreases and any 5'overhang at the 5' triphosphate end abolishes its activity. Upon ligand binding it associates with mitochondria antiviral signaling protein (MAVS/IPS1) which activates the IKK-related kinases: TBK1 and IKBKE which phosphorylate interferon regulatory factors: IRF3 and IRF7 which in turn activate transcription of antiviral immunological genes, including interferons (IFNs); IFN-alpha and IFN-beta. Detects both positive and negative strand RNA viruses including members of the families Paramyxoviridae: Human respiratory syncytial virus and measles virus (MeV), Rhabdoviridae: vesicular stomatitis virus (VSV), Orthomyxoviridae: influenza A and B virus, Flaviviridae: Japanese encephalitis virus (JEV), hepatitis C virus (HCV), dengue virus (DENV) and west Nile virus (WNV). It also detects rotavirus and reovirus. Also involved in antiviral signaling in response to viruses containing a dsDNA genome such as Epstein-Barr virus (EBV). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). May play important roles in granulocyte production and differentiation, bacterial phagocytosis and in the regulation of cell migration.13 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi810ZincPROSITE-ProRule annotation1
Metal bindingi813ZincPROSITE-ProRule annotation1
Metal bindingi864ZincPROSITE-ProRule annotation1
Metal bindingi869ZincPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi264 – 271ATPCurated8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • double-stranded DNA binding Source: Ensembl
  • double-stranded RNA binding Source: UniProtKB
  • helicase activity Source: UniProtKB-KW
  • identical protein binding Source: IntAct
  • single-stranded RNA binding Source: UniProtKB
  • ubiquitin protein ligase binding Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processAntiviral defense, Host-virus interaction, Immunity, Innate immunity
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-5689880 Ub-specific processing proteases
R-HSA-5689896 Ovarian tumor domain proteases
R-HSA-918233 TRAF3-dependent IRF activation pathway
R-HSA-933541 TRAF6 mediated IRF7 activation
R-HSA-933542 TRAF6 mediated NF-kB activation
R-HSA-933543 NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling
SIGNORiO95786

Names & Taxonomyi

Protein namesi
Recommended name:
Probable ATP-dependent RNA helicase DDX58 (EC:3.6.4.13)
Alternative name(s):
DEAD box protein 58
RIG-I-like receptor 1
Short name:
RLR-1
Retinoic acid-inducible gene 1 protein
Short name:
RIG-1
Retinoic acid-inducible gene I protein
Short name:
RIG-I
Gene namesi
Name:DDX58
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiHostDB:ENSG00000107201.9
HGNCiHGNC:19102 DDX58
MIMi609631 gene
neXtProtiNX_O95786

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Tight junction

Pathology & Biotechi

Involvement in diseasei

Singleton-Merten syndrome 2 (SGMRT2)1 Publication
The disease is caused by mutations affecting the gene represented in this entry.
Disease descriptionA form of Singleton-Merten syndrome, an autosomal dominant disorder characterized by marked aortic calcification, dental anomalies, osteopenia, acro-osteolysis, and to a lesser extent glaucoma, psoriasis, muscle weakness, and joint laxity. Additional clinical manifestations include particular facial characteristics and abnormal joint and muscle ligaments. SGMRT2 is an atypical form characterized by variable expression of glaucoma, aortic calcification, and skeletal abnormalities, without dental anomalies.
See also OMIM:616298
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_073667268C → F in SGMRT2; results in constitutive activation and enhanced interferon-mediated signaling. 1 PublicationCorresponds to variant dbSNP:rs786204848EnsemblClinVar.1
Natural variantiVAR_073668373E → A in SGMRT2; results in constitutive activation and enhanced interferon-mediated signaling. 1 PublicationCorresponds to variant dbSNP:rs786204847EnsemblClinVar.1

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi55T → I: No IRF3 signaling activity; no effect on dsRNA binding. 1 Publication1
Mutagenesisi99K → R: Little or no effect on ubiquitination of the 2 CARD domain. Abolishes ubiquitination by RNF125. 2 Publications1
Mutagenesisi154K → R: Reduction of ubiquitination. Reduction of INFB induction. 1 Publication1
Mutagenesisi164K → R: Reduction of ubiquitination. Reduction of INFB induction. 1 Publication1
Mutagenesisi169K → R: Little or no effect on ubiquitination of the 2 CARD domains. 1 Publication1
Mutagenesisi172K → R: Complete loss of ubiquitination; No interaction with MAVS/IPS1; No induction of IFN-beta. 2 Publications1
Mutagenesisi181K → R: Little or no effect on ubiquitination of the 2 CARD domains. 1 Publication1
Mutagenesisi190K → R: Little or no effect on ubiquitination of the 2 CARD domains. 1
Mutagenesisi193K → R: Little or no effect on ubiquitination of the 2 CARD domains. 1 Publication1
Mutagenesisi270K → A: No IRF3 signaling activity. 2 Publications1
Mutagenesisi495N → Q: Complete loss of herpes simplex virus 1 UL37-mediated deamidation; when associated with Q-549. 1 Publication1
Mutagenesisi549N → Q: Complete loss of herpes simplex virus 1 UL37-mediated deamidation; when associated with Q-495. 1 Publication1

Keywords - Diseasei

Disease mutation

Organism-specific databases

DisGeNETi23586
MalaCardsiDDX58
MIMi616298 phenotype
OpenTargetsiENSG00000107201
Orphaneti85191 Singleton-Merten dysplasia
PharmGKBiPA134994272

Polymorphism and mutation databases

BioMutaiDDX58

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001440931 – 925Probable ATP-dependent RNA helicase DDX58Add BLAST925

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki154Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki164Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki172Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Cross-linki181Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)1 Publication
Modified residuei495(Microbial infection) Deamidated asparagine; by herpes simplex virus 1/HHV-1 UL371 Publication1
Modified residuei549(Microbial infection) Deamidated asparagine; by herpes simplex virus 1/HHV-1 UL371 Publication1
Modified residuei770Phosphothreonine; by CK21 Publication1
Cross-linki812Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei854Phosphoserine; by CK21 Publication1
Modified residuei855Phosphoserine; by CK21 Publication1
Modified residuei858N6-acetyllysineCombined sources1

Post-translational modificationi

(Microbial infection) Deamidated on 'Asn-495' and 'Asn-549' by herpes simplex virus 1 protein UL37. These modifications eliminate DDX58 detection of viral RNA and restriction of viral replication.1 Publication
Phosphorylated in resting cells and dephosphorylated in RNA virus-infected cells. Phosphorylation at Thr-770, Ser-854 and Ser-855 results in inhibition of its activity while dephosphorylation at these sites results in its activation.1 Publication
ISGylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation. ISGylation negatively regulates its function in antiviral signaling response.3 Publications
Sumoylated, probably by MUL1; inhibiting its polyubiquitination.2 Publications
Ubiquitinated. Undergoes 'Lys-48'- and 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS/IPS1 binding and to induce anti-viral signal transduction (PubMed:17392790). Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated and TRIM4-mediated ubiquitination (PubMed:19017631, PubMed:19484123, PubMed:24755855). Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains (PubMed:18636086). Also probably deubiquitinated by USP17L2/USP17 that cleaves 'Lys-48'-and 'Lys-63'-linked ubiquitin chains and positively regulates the receptor (PubMed:20368735). Ubiquitinated at Lys-181 by RNF125, leading to its degradation: ubiquitination takes place upon viral infection and is enhanced 'Lys-63'-linked ubiquitination of the CARD domains, which promote interaction with VCP/p97 and subsequent recruitment of RNF125 (PubMed:17460044, PubMed:26471729). Ubiquitinated at Lys-812 by CBL, leading to its degradation: ubiquitination takes place upon viral infection and involves 'Lys-48'-linked ubiquitination (By similarity).By similarity8 Publications

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO95786
MaxQBiO95786
PaxDbiO95786
PeptideAtlasiO95786
PRIDEiO95786
ProteomicsDBi51047
51048 [O95786-2]

PTM databases

iPTMnetiO95786
PhosphoSitePlusiO95786

Expressioni

Tissue specificityi

Present in vascular smooth cells (at protein level).1 Publication

Inductioni

By bacterial lipopolysaccharides (LPS) in endothelial cells. By interferon (IFN).5 Publications

Gene expression databases

BgeeiENSG00000107201 Expressed in 216 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_DDX58
ExpressionAtlasiO95786 baseline and differential
GenevisibleiO95786 HS

Organism-specific databases

HPAiCAB012643
HPA047193

Interactioni

Subunit structurei

Monomer; maintained as a monomer in an autoinhibited state. Upon viral dsRNA binding and conformation shift, homomultimerizes and interacts (via tandem CARD domain) with MAVS/IPS1 promoting its filamentation. Interacts with DHX58/LGP2, IKBKE, TBK1 and TMEM173/STING. Interacts (via CARD domain) with TRIM25 (via SPRY domain). Interacts with RNF135. Interacts with CYLD. Interacts with NLRC5; blocks the interaction of MAVS/IPS1 to DDX58. Interacts with SRC. Interacts with DDX60. Interacts with isoform 2 of ZC3HAV1 (via zinc-fingers) in an RNA-dependent manner. Interacts (via tandem CARD domain) with SEC14L1; the interaction is direct and impairs the interaction of DDX58 with MAVS/IPS1. Interacts with VCP/p97; interaction is direct and leads to recruit RNF125 and subsequent ubiquitination and degradation (PubMed:26471729). Interacts with NOP53; may regulate DDX58 through USP15-mediated 'Lys-63'-linked deubiquitination (PubMed:27824081). Interacts with LRRC25 (PubMed:29288164).21 Publications
(Microbial infection) Interacts with protein Z of Guanarito virus, Machupo virus, Junin arenavirus and Sabia virus. This interaction disrupts its interaction with MAVS/IPS1, impeding downstream IRF3 and NF-kappa-B activation and resulting in decreased IFN-beta induction (PubMed:20007272).1 Publication
(Microbial infection) Interacts (via CARD domain) with Human respiratory syncytial virus A non-structural protein 2 (NS2) and this interaction disrupts its interaction with MAVS/IPS1, impeding downstream IRF3 activation (PubMed:19193793).1 Publication
(Microbial infection) Interacts with Rotavirus A non-structural protein 1 (NSP1) and this interaction induces down-regulation of DDX58/RIG-I (PubMed:22152002).1 Publication
(Microbial infection) Interacts with herpes simplex virus 1 protein US11; this interaction prevents the interaction of MAVS/IPS1 to DDX58 (PubMed:22301138).1 Publication
(Microbial infection) Interacts with herpes simplex virus 1 protein UL37; this interaction daeminates DDX58 and inhibits its activation.1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi117121, 43 interactors
CORUMiO95786
DIPiDIP-35444N
IntActiO95786, 32 interactors
MINTiO95786
STRINGi9606.ENSP00000369213

Structurei

Secondary structure

1925
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliO95786
SMRiO95786
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO95786

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 87CARD 1Add BLAST87
Domaini92 – 172CARD 2Add BLAST81
Domaini251 – 430Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST180
Domaini610 – 776Helicase C-terminalPROSITE-ProRule annotationAdd BLAST167
Domaini794 – 925RLR CTRPROSITE-ProRule annotationAdd BLAST132

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni218 – 925Interaction with ZC3HAV11 PublicationAdd BLAST708

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi372 – 375DECH box4

Domaini

The RLR CTR domain controls homomultimerization and interaction with MAVS/IPS1. In the absence of viral infection, the protein is maintained as a monomer in an autoinhibited state with the CARD domains masked through intramolecular interactions mediated by the RLR CTR domain. Upon binding to viral RNA in the presence of ATP, the RLR CTR domain induces a conformational change exposing the CARD domain and promotes dimerization and CARD interactions with the adapter protein MAVS/IPS1 leading to the induction of downstream signaling.
The helicase domain is responsible for dsRNA recognition.
The 2 CARD domains are responsible for interaction with and signaling through MAVS/IPS1 and for association with the actin cytoskeleton.
The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.1 Publication

Sequence similaritiesi

Belongs to the helicase family. RLR subfamily.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0354 Eukaryota
COG1111 LUCA
GeneTreeiENSGT00510000046789
HOGENOMiHOG000230911
HOVERGENiHBG052325
InParanoidiO95786
KOiK12646
OMAiLISQECE
OrthoDBiEOG091G01PQ
PhylomeDBiO95786
TreeFamiTF330258

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di2.170.150.30, 1 hit
InterProiView protein in InterPro
IPR031964 CARD_dom
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038557 RLR_C_sf
IPR021673 RLR_CTR
PfamiView protein in Pfam
PF16739 CARD_2, 2 hits
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF11648 RIG-I_C-RD, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51789 RLR_CTR, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: O95786-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MTTEQRRSLQ AFQDYIRKTL DPTYILSYMA PWFREEEVQY IQAEKNNKGP
60 70 80 90 100
MEAATLFLKF LLELQEEGWF RGFLDALDHA GYSGLYEAIE SWDFKKIEKL
110 120 130 140 150
EEYRLLLKRL QPEFKTRIIP TDIISDLSEC LINQECEEIL QICSTKGMMA
160 170 180 190 200
GAEKLVECLL RSDKENWPKT LKLALEKERN KFSELWIVEK GIKDVETEDL
210 220 230 240 250
EDKMETSDIQ IFYQEDPECQ NLSENSCPPS EVSDTNLYSP FKPRNYQLEL
260 270 280 290 300
ALPAMKGKNT IICAPTGCGK TFVSLLICEH HLKKFPQGQK GKVVFFANQI
310 320 330 340 350
PVYEQQKSVF SKYFERHGYR VTGISGATAE NVPVEQIVEN NDIIILTPQI
360 370 380 390 400
LVNNLKKGTI PSLSIFTLMI FDECHNTSKQ HPYNMIMFNY LDQKLGGSSG
410 420 430 440 450
PLPQVIGLTA SVGVGDAKNT DEALDYICKL CASLDASVIA TVKHNLEELE
460 470 480 490 500
QVVYKPQKFF RKVESRISDK FKYIIAQLMR DTESLAKRIC KDLENLSQIQ
510 520 530 540 550
NREFGTQKYE QWIVTVQKAC MVFQMPDKDE ESRICKALFL YTSHLRKYND
560 570 580 590 600
ALIISEHARM KDALDYLKDF FSNVRAAGFD EIEQDLTQRF EEKLQELESV
610 620 630 640 650
SRDPSNENPK LEDLCFILQE EYHLNPETIT ILFVKTRALV DALKNWIEGN
660 670 680 690 700
PKLSFLKPGI LTGRGKTNQN TGMTLPAQKC ILDAFKASGD HNILIATSVA
710 720 730 740 750
DEGIDIAQCN LVILYEYVGN VIKMIQTRGR GRARGSKCFL LTSNAGVIEK
760 770 780 790 800
EQINMYKEKM MNDSILRLQT WDEAVFREKI LHIQTHEKFI RDSQEKPKPV
810 820 830 840 850
PDKENKKLLC RKCKALACYT ADVRVIEECH YTVLGDAFKE CFVSRPHPKP
860 870 880 890 900
KQFSSFEKRA KIFCARQNCS HDWGIHVKYK TFEIPVIKIE SFVVEDIATG
910 920
VQTLYSKWKD FHFEKIPFDP AEMSK
Length:925
Mass (Da):106,600
Last modified:November 8, 2005 - v2
Checksum:iBF0D501C395BAE25
GO
Isoform 2 (identifier: O95786-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     36-80: Missing.

Note: No experimental confirmation available.
Show »
Length:880
Mass (Da):101,377
Checksum:i4B1603B6F2F37A66
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A2A376A2A376_HUMAN
Probable ATP-dependent RNA helicase...
DDX58
722Annotation score:

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_0237477R → C1 PublicationCorresponds to variant dbSNP:rs10813831Ensembl.1
Natural variantiVAR_073667268C → F in SGMRT2; results in constitutive activation and enhanced interferon-mediated signaling. 1 PublicationCorresponds to variant dbSNP:rs786204848EnsemblClinVar.1
Natural variantiVAR_073668373E → A in SGMRT2; results in constitutive activation and enhanced interferon-mediated signaling. 1 PublicationCorresponds to variant dbSNP:rs786204847EnsemblClinVar.1
Natural variantiVAR_023748580D → E2 PublicationsCorresponds to variant dbSNP:rs17217280Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01605436 – 80Missing in isoform 2. 1 PublicationAdd BLAST45

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038963 mRNA Translation: AAD19826.1
AL161783 Genomic DNA No translation available.
AL353671 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58548.1
BC132786 mRNA Translation: AAI32787.1
BC136610 mRNA Translation: AAI36611.1
BX647917 mRNA Translation: CAI46068.1
AL137608 mRNA Translation: CAB70840.1
CCDSiCCDS6526.1 [O95786-1]
PIRiT46312
RefSeqiNP_055129.2, NM_014314.3 [O95786-1]
UniGeneiHs.190622

Genome annotation databases

EnsembliENST00000379883; ENSP00000369213; ENSG00000107201 [O95786-1]
GeneIDi23586
KEGGihsa:23586
UCSCiuc003zra.4 human [O95786-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038963 mRNA Translation: AAD19826.1
AL161783 Genomic DNA No translation available.
AL353671 Genomic DNA No translation available.
CH471071 Genomic DNA Translation: EAW58548.1
BC132786 mRNA Translation: AAI32787.1
BC136610 mRNA Translation: AAI36611.1
BX647917 mRNA Translation: CAI46068.1
AL137608 mRNA Translation: CAB70840.1
CCDSiCCDS6526.1 [O95786-1]
PIRiT46312
RefSeqiNP_055129.2, NM_014314.3 [O95786-1]
UniGeneiHs.190622

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2LWDNMR-A95-190[»]
2LWENMR-A95-190[»]
2QFBX-ray3.00A/B/C/D/E/F/G/H/I/J802-925[»]
2QFDX-ray2.70A/B/C/D/E/F/G/H/I/J802-925[»]
2RMJNMR-A792-925[»]
2YKGX-ray2.50A230-925[»]
3LRNX-ray2.60A/B803-923[»]
3LRRX-ray2.15A/B803-923[»]
3NCUX-ray2.55A/B792-925[»]
3OG8X-ray2.40A/B802-925[»]
3ZD6X-ray2.80A230-925[»]
3ZD7X-ray2.50A230-925[»]
4AY2X-ray2.80A239-925[»]
4BPBX-ray2.58A230-925[»]
4NQKX-ray3.70A/B/C/D1-200[»]
4ON9X-ray2.71A/B230-793[»]
4P4HX-ray3.40A/B/C/D/E/F/G/H1-201[»]
5E3HX-ray2.70A232-925[»]
5F98X-ray3.28A/C/E/G/I/K232-925[»]
5F9FX-ray2.60A/C/E/G/I/K232-925[»]
5F9HX-ray3.10A/C/E/G/I/K232-925[»]
6GPGX-ray2.89A232-925[»]
ProteinModelPortaliO95786
SMRiO95786
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi117121, 43 interactors
CORUMiO95786
DIPiDIP-35444N
IntActiO95786, 32 interactors
MINTiO95786
STRINGi9606.ENSP00000369213

PTM databases

iPTMnetiO95786
PhosphoSitePlusiO95786

Polymorphism and mutation databases

BioMutaiDDX58

Proteomic databases

EPDiO95786
MaxQBiO95786
PaxDbiO95786
PeptideAtlasiO95786
PRIDEiO95786
ProteomicsDBi51047
51048 [O95786-2]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000379883; ENSP00000369213; ENSG00000107201 [O95786-1]
GeneIDi23586
KEGGihsa:23586
UCSCiuc003zra.4 human [O95786-1]

Organism-specific databases

CTDi23586
DisGeNETi23586
EuPathDBiHostDB:ENSG00000107201.9
GeneCardsiDDX58
HGNCiHGNC:19102 DDX58
HPAiCAB012643
HPA047193
MalaCardsiDDX58
MIMi609631 gene
616298 phenotype
neXtProtiNX_O95786
OpenTargetsiENSG00000107201
Orphaneti85191 Singleton-Merten dysplasia
PharmGKBiPA134994272
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0354 Eukaryota
COG1111 LUCA
GeneTreeiENSGT00510000046789
HOGENOMiHOG000230911
HOVERGENiHBG052325
InParanoidiO95786
KOiK12646
OMAiLISQECE
OrthoDBiEOG091G01PQ
PhylomeDBiO95786
TreeFamiTF330258

Enzyme and pathway databases

ReactomeiR-HSA-1169408 ISG15 antiviral mechanism
R-HSA-168928 DDX58/IFIH1-mediated induction of interferon-alpha/beta
R-HSA-5689880 Ub-specific processing proteases
R-HSA-5689896 Ovarian tumor domain proteases
R-HSA-918233 TRAF3-dependent IRF activation pathway
R-HSA-933541 TRAF6 mediated IRF7 activation
R-HSA-933542 TRAF6 mediated NF-kB activation
R-HSA-933543 NF-kB activation through FADD/RIP-1 pathway mediated by caspase-8 and -10
R-HSA-936440 Negative regulators of DDX58/IFIH1 signaling
SIGNORiO95786

Miscellaneous databases

ChiTaRSiDDX58 human
EvolutionaryTraceiO95786
GeneWikiiRIG-I
GenomeRNAii23586
PROiPR:O95786
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000107201 Expressed in 216 organ(s), highest expression level in buccal mucosa cell
CleanExiHS_DDX58
ExpressionAtlasiO95786 baseline and differential
GenevisibleiO95786 HS

Family and domain databases

CDDicd00079 HELICc, 1 hit
Gene3Di2.170.150.30, 1 hit
InterProiView protein in InterPro
IPR031964 CARD_dom
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR038557 RLR_C_sf
IPR021673 RLR_CTR
PfamiView protein in Pfam
PF16739 CARD_2, 2 hits
PF00270 DEAD, 1 hit
PF00271 Helicase_C, 1 hit
PF11648 RIG-I_C-RD, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
PS51789 RLR_CTR, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDDX58_HUMAN
AccessioniPrimary (citable) accession number: O95786
Secondary accession number(s): A2RU81
, Q5HYE1, Q5VYT1, Q9NT04
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 8, 2005
Last sequence update: November 8, 2005
Last modified: November 7, 2018
This is version 165 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human chromosome 9
    Human chromosome 9: entries, gene names and cross-references to MIM
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