UniProtKB - O95782 (AP2A1_HUMAN)
AP-2 complex subunit alpha-1
AP2A1
Functioni
GO - Molecular functioni
- cargo adaptor activity Source: GO_Central
- clathrin adaptor activity Source: GO_Central
- low-density lipoprotein particle receptor binding Source: ARUK-UCL
- protein-containing complex binding Source: Ensembl
- protein C-terminus binding Source: Ensembl
- protein kinase binding Source: ParkinsonsUK-UCL
GO - Biological processi
- antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
- clathrin-dependent endocytosis Source: UniProtKB
- endocytosis Source: UniProtKB
- ephrin receptor signaling pathway Source: Reactome
- Golgi to endosome transport Source: UniProtKB
- intracellular protein transport Source: UniProtKB
- low-density lipoprotein particle clearance Source: Reactome
- low-density lipoprotein particle receptor catabolic process Source: Reactome
- membrane organization Source: Reactome
- negative regulation of hyaluronan biosynthetic process Source: UniProtKB
- positive regulation of neuron projection development Source: Ensembl
- positive regulation of receptor-mediated endocytosis Source: Ensembl
- receptor-mediated endocytosis Source: GO_Central
- regulation of defense response to virus by virus Source: Reactome
- Wnt signaling pathway, planar cell polarity pathway Source: Reactome
Keywordsi
Biological process | Endocytosis, Protein transport, Transport |
Enzyme and pathway databases
PathwayCommonsi | O95782 |
Reactomei | R-HSA-167590, Nef Mediated CD4 Down-regulation R-HSA-177504, Retrograde neurotrophin signalling R-HSA-182218, Nef Mediated CD8 Down-regulation R-HSA-2132295, MHC class II antigen presentation R-HSA-3928665, EPH-ephrin mediated repulsion of cells R-HSA-416993, Trafficking of GluR2-containing AMPA receptors R-HSA-437239, Recycling pathway of L1 R-HSA-5099900, WNT5A-dependent internalization of FZD4 R-HSA-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2 R-HSA-8856825, Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828, Clathrin-mediated endocytosis R-HSA-8866427, VLDLR internalisation and degradation R-HSA-8964038, LDL clearance R-HSA-9679191, Potential therapeutics for SARS |
SignaLinki | O95782 |
Protein family/group databases
TCDBi | 9.B.278.1.4, the organellar-targeting adaptor protein complex (o-apc) family |
Names & Taxonomyi
Protein namesi | Recommended name: AP-2 complex subunit alpha-1Alternative name(s): 100 kDa coated vesicle protein A Adaptor protein complex AP-2 subunit alpha-1 Adaptor-related protein complex 2 subunit alpha-1 Alpha-adaptin A Alpha1-adaptin Clathrin assembly protein complex 2 alpha-A large chain Plasma membrane adaptor HA2/AP2 adaptin alpha A subunit |
Gene namesi | Name:AP2A1 Synonyms:ADTAA, CLAPA1 |
Organismi | Homo sapiens (Human) |
Taxonomic identifieri | 9606 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Proteomesi |
|
Organism-specific databases
HGNCi | HGNC:561, AP2A1 |
MIMi | 601026, gene |
neXtProti | NX_O95782 |
VEuPathDBi | HostDB:ENSG00000196961.12 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication
Other locations
- coated pit By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: AP-2 appears to be excluded from internalizing CCVs and to disengage from sites of endocytosis seconds before internalization of the nascent CCV.By similarity
Cytosol
- cytosol Source: Reactome
Endosome
- endolysosome membrane Source: Reactome
Golgi apparatus
- clathrin coat of trans-Golgi network vesicle Source: UniProtKB
Lysosome
- endolysosome membrane Source: Reactome
Plasma Membrane
- AP-2 adaptor complex Source: UniProtKB
- apical plasma membrane Source: Ensembl
- basolateral plasma membrane Source: Ensembl
- plasma membrane Source: Reactome
Other locations
- clathrin-coated endocytic vesicle Source: ARUK-UCL
- clathrin-coated endocytic vesicle membrane Source: Reactome
- endocytic vesicle membrane Source: Reactome
- filopodium tip Source: Ensembl
- membrane Source: UniProtKB
Keywords - Cellular componenti
Cell membrane, Coated pit, MembranePathology & Biotechi
Organism-specific databases
DisGeNETi | 160 |
OpenTargetsi | ENSG00000196961 |
PharmGKBi | PA24852 |
Miscellaneous databases
Pharosi | O95782, Tbio |
Chemistry databases
ChEMBLi | CHEMBL4295687 |
Genetic variation databases
BioMutai | AP2A1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193730 | 1 – 977 | AP-2 complex subunit alpha-1Add BLAST | 977 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 626 | PhosphoserineCombined sources | 1 | |
Modified residuei | 652 | PhosphoserineCombined sources | 1 | |
Modified residuei | 653 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 655 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | O95782 |
jPOSTi | O95782 |
MassIVEi | O95782 |
MaxQBi | O95782 |
PaxDbi | O95782 |
PeptideAtlasi | O95782 |
PRIDEi | O95782 |
ProteomicsDBi | 51043 [O95782-1] 51044 [O95782-2] |
PTM databases
iPTMneti | O95782 |
MetOSitei | O95782 |
PhosphoSitePlusi | O95782 |
SwissPalmi | O95782 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSG00000196961, Expressed in right adrenal gland and 203 other tissues |
ExpressionAtlasi | O95782, baseline and differential |
Genevisiblei | O95782, HS |
Organism-specific databases
HPAi | ENSG00000196961, Low tissue specificity |
Interactioni
Subunit structurei
Adaptor protein complex 2 (AP-2) is a heterotetramer composed of two large adaptins (alpha-type subunit AP2A1 or AP2A2 and beta-type subunit AP2B1), a medium adaptin (mu-type subunit AP2M1) and a small adaptin (sigma-type subunit AP2S1).
Interacts with SGIP1 (By similarity).
Interacts with HIP1 and RAB11FIP2 (PubMed:11532990, PubMed:12364336).
Interacts with SLC12A5 (By similarity).
Interacts with clathrin (By similarity).
Interacts with RFTN1 (PubMed:27022195).
Interacts with KIAA1107 (PubMed:29262337).
Interacts with PICALM (PubMed:16262731). Together with AP2B1 and AP2M1, it interacts with ADAM10; this interaction facilitates ADAM10 endocytosis from the plasma membrane during long-term potentiation in hippocampal neurons (PubMed:23676497).
Interacts with ABCB11; this interaction regulates cell membrane expresion of ABCB11 through its internalization in a clathrin-dependent manner and its subsequent degradation (PubMed:22262466).
By similarity7 PublicationsGO - Molecular functioni
- clathrin adaptor activity Source: GO_Central
- low-density lipoprotein particle receptor binding Source: ARUK-UCL
- protein C-terminus binding Source: Ensembl
- protein kinase binding Source: ParkinsonsUK-UCL
Protein-protein interaction databases
BioGRIDi | 106669, 182 interactors |
ComplexPortali | CPX-5149, AP-2 Adaptor complex, alpha1 variant |
CORUMi | O95782 |
DIPi | DIP-33164N |
ELMi | O95782 |
IntActi | O95782, 107 interactors |
MINTi | O95782 |
STRINGi | 9606.ENSP00000351926 |
Miscellaneous databases
RNActi | O95782, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1077, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_1_0_1 |
InParanoidi | O95782 |
OMAi | HNELFNC |
OrthoDBi | 751651at2759 |
PhylomeDBi | O95782 |
TreeFami | TF300308 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 3.30.310.10, 1 hit |
InterProi | View protein in InterPro IPR017104, AP2_complex_asu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR003164, Clathrin_a-adaptin_app_sub_C IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR009028, Coatomer/calthrin_app_sub_C IPR012295, TBP_dom_sf |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02296, Alpha_adaptin_C, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037091, AP2_complex_alpha, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit SSF55711, SSF55711, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MPAVSKGDGM RGLAVFISDI RNCKSKEAEI KRINKELANI RSKFKGDKAL
60 70 80 90 100
DGYSKKKYVC KLLFIFLLGH DIDFGHMEAV NLLSSNKYTE KQIGYLFISV
110 120 130 140 150
LVNSNSELIR LINNAIKNDL ASRNPTFMCL ALHCIANVGS REMGEAFAAD
160 170 180 190 200
IPRILVAGDS MDSVKQSAAL CLLRLYKASP DLVPMGEWTA RVVHLLNDQH
210 220 230 240 250
MGVVTAAVSL ITCLCKKNPD DFKTCVSLAV SRLSRIVSSA STDLQDYTYY
260 270 280 290 300
FVPAPWLSVK LLRLLQCYPP PEDAAVKGRL VECLETVLNK AQEPPKSKKV
310 320 330 340 350
QHSNAKNAIL FETISLIIHY DSEPNLLVRA CNQLGQFLQH RETNLRYLAL
360 370 380 390 400
ESMCTLASSE FSHEAVKTHI DTVINALKTE RDVSVRQRAA DLLYAMCDRS
410 420 430 440 450
NAKQIVSEML RYLETADYAI REEIVLKVAI LAEKYAVDYS WYVDTILNLI
460 470 480 490 500
RIAGDYVSEE VWYRVLQIVT NRDDVQGYAA KTVFEALQAP ACHENMVKVG
510 520 530 540 550
GYILGEFGNL IAGDPRSSPP VQFSLLHSKF HLCSVATRAL LLSTYIKFIN
560 570 580 590 600
LFPETKATIQ GVLRAGSQLR NADVELQQRA VEYLTLSSVA STDVLATVLE
610 620 630 640 650
EMPPFPERES SILAKLKRKK GPGAGSALDD GRRDPSSNDI NGGMEPTPST
660 670 680 690 700
VSTPSPSADL LGLRAAPPPA APPASAGAGN LLVDVFDGPA AQPSLGPTPE
710 720 730 740 750
EAFLSELEPP APESPMALLA DPAPAADPGP EDIGPPIPEA DELLNKFVCK
760 770 780 790 800
NNGVLFENQL LQIGVKSEFR QNLGRMYLFY GNKTSVQFQN FSPTVVHPGD
810 820 830 840 850
LQTQLAVQTK RVAAQVDGGA QVQQVLNIEC LRDFLTPPLL SVRFRYGGAP
860 870 880 890 900
QALTLKLPVT INKFFQPTEM AAQDFFQRWK QLSLPQQEAQ KIFKANHPMD
910 920 930 940 950
AEVTKAKLLG FGSALLDNVD PNPENFVGAG IIQTKALQVG CLLRLEPNAQ
960 970
AQMYRLTLRT SKEPVSRHLC ELLAQQF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketM0R2D9 | M0R2D9_HUMAN | AP-2 complex subunit alpha-1 | AP2A1 | 57 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 804 | Q → H in AAL11039 (PubMed:12036598).Curated | 1 | |
Sequence conflicti | 804 | Q → H in AAL11040 (PubMed:12036598).Curated | 1 | |
Sequence conflicti | 924 – 977 | ENFVG…LAQQF → GDREDTRVWGMPGTFLRPFV FLFLFICCCLHSGGLGGVPL PPFPPQAQRGEGPGKWMSPP LPPHPVVAPPTPSPSRGCVL L in AAH14214 (PubMed:15489334).CuratedAdd BLAST | 54 |
Natural variant
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Natural variantiVAR_060544 | 270 | P → L1 PublicationCorresponds to variant dbSNP:rs17851121Ensembl. | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_000161 | 706 – 727 | Missing in isoform B. 2 PublicationsAdd BLAST | 22 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF289221 Genomic DNA Translation: AAL11039.1 AF289221 Genomic DNA Translation: AAL11040.1 AL136925 mRNA Translation: CAB66859.1 AC006942 Genomic DNA Translation: AAD15564.1 Sequence problems. AC011495 Genomic DNA No translation available. AC098783 Genomic DNA No translation available. BC014214 mRNA Translation: AAH14214.1 |
CCDSi | CCDS46148.1 [O95782-1] CCDS46149.1 [O95782-2] |
RefSeqi | NP_055018.2, NM_014203.2 [O95782-1] NP_570603.2, NM_130787.2 [O95782-2] |
Genome annotation databases
Ensembli | ENST00000354293; ENSP00000346246; ENSG00000196961 [O95782-2] ENST00000359032; ENSP00000351926; ENSG00000196961 [O95782-1] |
GeneIDi | 160 |
KEGGi | hsa:160 |
UCSCi | uc002ppn.4, human [O95782-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF289221 Genomic DNA Translation: AAL11039.1 AF289221 Genomic DNA Translation: AAL11040.1 AL136925 mRNA Translation: CAB66859.1 AC006942 Genomic DNA Translation: AAD15564.1 Sequence problems. AC011495 Genomic DNA No translation available. AC098783 Genomic DNA No translation available. BC014214 mRNA Translation: AAH14214.1 |
CCDSi | CCDS46148.1 [O95782-1] CCDS46149.1 [O95782-2] |
RefSeqi | NP_055018.2, NM_014203.2 [O95782-1] NP_570603.2, NM_130787.2 [O95782-2] |
3D structure databases
SMRi | O95782 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 106669, 182 interactors |
ComplexPortali | CPX-5149, AP-2 Adaptor complex, alpha1 variant |
CORUMi | O95782 |
DIPi | DIP-33164N |
ELMi | O95782 |
IntActi | O95782, 107 interactors |
MINTi | O95782 |
STRINGi | 9606.ENSP00000351926 |
Chemistry databases
ChEMBLi | CHEMBL4295687 |
Protein family/group databases
TCDBi | 9.B.278.1.4, the organellar-targeting adaptor protein complex (o-apc) family |
PTM databases
iPTMneti | O95782 |
MetOSitei | O95782 |
PhosphoSitePlusi | O95782 |
SwissPalmi | O95782 |
Genetic variation databases
BioMutai | AP2A1 |
Proteomic databases
EPDi | O95782 |
jPOSTi | O95782 |
MassIVEi | O95782 |
MaxQBi | O95782 |
PaxDbi | O95782 |
PeptideAtlasi | O95782 |
PRIDEi | O95782 |
ProteomicsDBi | 51043 [O95782-1] 51044 [O95782-2] |
Protocols and materials databases
Antibodypediai | 3805, 284 antibodies |
DNASUi | 160 |
Genome annotation databases
Ensembli | ENST00000354293; ENSP00000346246; ENSG00000196961 [O95782-2] ENST00000359032; ENSP00000351926; ENSG00000196961 [O95782-1] |
GeneIDi | 160 |
KEGGi | hsa:160 |
UCSCi | uc002ppn.4, human [O95782-1] |
Organism-specific databases
CTDi | 160 |
DisGeNETi | 160 |
GeneCardsi | AP2A1 |
HGNCi | HGNC:561, AP2A1 |
HPAi | ENSG00000196961, Low tissue specificity |
MIMi | 601026, gene |
neXtProti | NX_O95782 |
OpenTargetsi | ENSG00000196961 |
PharmGKBi | PA24852 |
VEuPathDBi | HostDB:ENSG00000196961.12 |
GenAtlasi | Search... |
Phylogenomic databases
eggNOGi | KOG1077, Eukaryota |
GeneTreei | ENSGT00950000182838 |
HOGENOMi | CLU_003824_1_0_1 |
InParanoidi | O95782 |
OMAi | HNELFNC |
OrthoDBi | 751651at2759 |
PhylomeDBi | O95782 |
TreeFami | TF300308 |
Enzyme and pathway databases
PathwayCommonsi | O95782 |
Reactomei | R-HSA-167590, Nef Mediated CD4 Down-regulation R-HSA-177504, Retrograde neurotrophin signalling R-HSA-182218, Nef Mediated CD8 Down-regulation R-HSA-2132295, MHC class II antigen presentation R-HSA-3928665, EPH-ephrin mediated repulsion of cells R-HSA-416993, Trafficking of GluR2-containing AMPA receptors R-HSA-437239, Recycling pathway of L1 R-HSA-5099900, WNT5A-dependent internalization of FZD4 R-HSA-5140745, WNT5A-dependent internalization of FZD2, FZD5 and ROR2 R-HSA-8856825, Cargo recognition for clathrin-mediated endocytosis R-HSA-8856828, Clathrin-mediated endocytosis R-HSA-8866427, VLDLR internalisation and degradation R-HSA-8964038, LDL clearance R-HSA-9679191, Potential therapeutics for SARS |
SignaLinki | O95782 |
Miscellaneous databases
BioGRID-ORCSi | 160, 37 hits in 874 CRISPR screens |
ChiTaRSi | AP2A1, human |
GeneWikii | Adaptor-related_protein_complex_2,_alpha_1 |
GenomeRNAii | 160 |
Pharosi | O95782, Tbio |
PROi | PR:O95782 |
RNActi | O95782, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSG00000196961, Expressed in right adrenal gland and 203 other tissues |
ExpressionAtlasi | O95782, baseline and differential |
Genevisiblei | O95782, HS |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit 3.30.310.10, 1 hit |
InterProi | View protein in InterPro IPR017104, AP2_complex_asu IPR011989, ARM-like IPR016024, ARM-type_fold IPR002553, Clathrin/coatomer_adapt-like_N IPR003164, Clathrin_a-adaptin_app_sub_C IPR008152, Clathrin_a/b/g-adaptin_app_Ig IPR013041, Clathrin_app_Ig-like_sf IPR009028, Coatomer/calthrin_app_sub_C IPR012295, TBP_dom_sf |
Pfami | View protein in Pfam PF01602, Adaptin_N, 1 hit PF02296, Alpha_adaptin_C, 1 hit PF02883, Alpha_adaptinC2, 1 hit |
PIRSFi | PIRSF037091, AP2_complex_alpha, 1 hit |
SMARTi | View protein in SMART SM00809, Alpha_adaptinC2, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit SSF49348, SSF49348, 1 hit SSF55711, SSF55711, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AP2A1_HUMAN | |
Accessioni | O95782Primary (citable) accession number: O95782 Secondary accession number(s): Q96CI7 Q9H070 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 24, 2001 |
Last sequence update: | November 3, 2009 | |
Last modified: | February 10, 2021 | |
This is version 193 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program | |
Disclaimer | Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care. |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Human chromosome 19
Human chromosome 19: entries, gene names and cross-references to MIM - Human entries with genetic variants
List of human entries with genetic variants - Human variants curated from literature reports
Index of human variants curated from literature reports - MIM cross-references
Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families